Restriction map for
pF1KE0881
(Circular) (Six-base) MAPSORT of: pF1KE0881.seq Check: 1501 from: 1 to: 4040
pF1KE0881 4040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AatII G_ACGT'C
Cuts at: 683 683
Size: 4040
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 999 1865 2297 3044 3312 682
Size: 317 866 432 747 268 1410
Fragments arranged by size:
1410 866 747 432 317 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 1044 3393 3649 1044
Size: 2349 256 1435
Fragments arranged by size:
2349 1435 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
AvrII C'CTAG_G
Cuts at: 708 708
Size: 4040
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 1056 1470 1718 1753 1056
Size: 414 248 35 3343
Fragments arranged by size:
3343 414 248 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbsI GAAGACnn'nnnn_
Cuts at: 177 771 177
Size: 594 3446
BciVI GTATCCnnnnn_n'
Cuts at: 541 1938 2699 4040 541
Size: 1397 761 1341 541
Fragments arranged by size:
1397 1341 761 541
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BfrBI ATG'CAT
Cuts at: 743 743
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 1665 1758 2814 1665
Size: 93 1056 2891
Fragments arranged by size:
2891 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 680 1471 1719 2421 3917 680
Size: 791 248 702 1496 803
Fragments arranged by size:
1496 803 791 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1020 1020
Size: 4040
BseYI C'CCAG_C
Cuts at: 544 948 1465 2223 2800 3296 544
Size: 404 517 758 577 496 1288
Fragments arranged by size:
1288 758 577 517 496 404
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 804 804
Size: 4040
BsiEI CG_ry'CG
Cuts at: 104 471 1582 1628 2836 104
Size: 367 1111 46 1208 1308
Fragments arranged by size:
1308 1208 1111 367 46
BsiHKAI G_wGCw'C
Cuts at: 576 1054 1832 2022 2814 576
Size: 478 778 190 792 1802
Fragments arranged by size:
1802 792 778 478 190
Bsp1286I G_dGCh'C
Cuts at: 576 1054 1665 1758 1832 2022 2084 2814 576
Size: 478 611 93 74 190 62 730 1802
Fragments arranged by size:
1802 730 611 478 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 416 3216 416
Size: 2800 1240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1090 1304 1606 1987 1090
Size: 214 302 381 3143
Fragments arranged by size:
3143 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 464 1952 464
Size: 1488 2552
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 1065 1532 1555 1890 2136 3148 3234 3246
Size: 1064 467 23 335 246 1012 86 12
Cuts at: 3246 3592 1
Size: 346 449
Fragments arranged by size:
1064 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 344 344
Size: 4040
BtgI C'CryG_G
Cuts at: 520 520
Size: 4040
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 923 947 1283 923
Size: 24 336 3680
Fragments arranged by size:
3680 336 24
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 248 353 428 479 1625 1799 2190 2217 3562
Size: 105 75 51 1146 174 391 27 1345
Cuts at: 3562 248
Size: 726
Fragments arranged by size:
1345 1146 726 391 174 105 75 51
27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 1018 2063 2273 1018
Size: 1045 210 2785
Fragments arranged by size:
2785 1045 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 874 2248 2558 2704 3850 874
Size: 1374 310 146 1146 1064
Fragments arranged by size:
1374 1146 1064 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 999 1865 2297 3044 3312 682
Size: 317 866 432 747 268 1410
Fragments arranged by size:
1410 866 747 432 317 268
EcoO109I rG'GnC_Cy
Cuts at: 1179 1179
Size: 4040
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 216 248 1882 1912 1914 1944 216
Size: 32 1634 30 2 30 2312
Fragments arranged by size:
2312 1634 32 30 30 2
HincII GTy'rAC
Cuts at: 315 1079 315
Size: 764 3276
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 250 355 430 481 1801 250
Size: 105 75 51 1320 2489
Fragments arranged by size:
2489 1320 105 75 51
MslI CAynn'nnrTG
Cuts at: 689 738 2029 2156 689
Size: 49 1291 127 2573
Fragments arranged by size:
2573 1291 127 49
MspA1I CmG'CkG
Cuts at: 488 1151 1465 1825 2838 3083 488
Size: 663 314 360 1013 245 1445
Fragments arranged by size:
1445 1013 663 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NsiI A_TGCA'T
Cuts at: 745 745
Size: 4040
NspI r_CATG'y
Cuts at: 879 1093 1593 2500 879
Size: 214 500 907 2419
Fragments arranged by size:
2419 907 500 214
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 366 366
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PstI C_TGCA'G
Cuts at: 1087 1772 1087
Size: 685 3355
PvuI CG_AT'CG
Cuts at: 104 471 104
Size: 367 3673
PvuII CAG'CTG
Cuts at: 488 1465 1825 488
Size: 977 360 2703
Fragments arranged by size:
2703 977 360
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 969 1003 1083 1768 2761 2952 33
Size: 936 34 80 685 993 191 1121
Fragments arranged by size:
1121 993 936 685 191 80 34
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 1195 2110 2602 2864 1195
Size: 915 492 262 2371
Fragments arranged by size:
2371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 160 67
Size: 93 3947
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 308 425 431 449 708 1174 1528 308
Size: 117 6 18 259 466 354 2820
Fragments arranged by size:
2820 466 354 259 117 18 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 543 1623 1756 2215 2424 543
Size: 1080 133 459 209 2159
Fragments arranged by size:
2159 1080 459 209 133
TatI w'GTAC_w
Cuts at: 3555 3555
Size: 4040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 363 1535 306
Size: 57 1172 2811
Fragments arranged by size:
2811 1172 57
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
XmnI GAAnn'nnTTC
Cuts at: 1012 1012
Size: 4040
ZraI GAC'GTC
Cuts at: 681 681
Size: 4040
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI