Restriction map for
pF1KE0880
(Circular) (Six-base) MAPSORT of: pF1KE0880.seq Check: 2286 from: 1 to: 4040
pF1KE0880 4040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1865 2297 3044 3312 682
Size: 1183 432 747 268 1410
Fragments arranged by size:
1410 1183 747 432 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AleI CACnn'nnGTG
Cuts at: 940 940
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 139 957 1044 3393 3649 139
Size: 818 87 2349 256 530
Fragments arranged by size:
2349 818 530 256 87
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
AvrII C'CTAG_G
Cuts at: 708 708
Size: 4040
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 1056 1470 1718 1753 1056
Size: 414 248 35 3343
Fragments arranged by size:
3343 414 248 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbsI GAAGACnn'nnnn_
Cuts at: 759 771 759
Size: 12 4028
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 920 1560 920
Size: 640 3400
BfrBI ATG'CAT
Cuts at: 743 743
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 877 1665 1758 2814 877
Size: 788 93 1056 2103
Fragments arranged by size:
2103 1056 788 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1216 2095 2587 2885 793
Size: 423 879 492 298 1948
Fragments arranged by size:
1948 879 492 423 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 878 1393 878
Size: 515 3525
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1020 1020
Size: 4040
BseYI C'CCAG_C
Cuts at: 544 1465 2223 2800 3296 544
Size: 921 758 577 496 1288
Fragments arranged by size:
1288 921 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 809 1054 1832 2022 2814 809
Size: 245 778 190 792 2035
Fragments arranged by size:
2035 792 778 245 190
Bsp1286I G_dGCh'C
Cuts at: 809 877 1054 1665 1758 1832 2022 2084 2814
Size: 68 177 611 93 74 190 62 730
Cuts at: 2814 809
Size: 2035
Fragments arranged by size:
2035 730 611 190 177 93 74 68
62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 416 3216 416
Size: 2800 1240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 1090 1304 1606 1987 242
Size: 848 214 302 381 2295
Fragments arranged by size:
2295 848 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 464 1952 464
Size: 1488 2552
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BsrGI T'GTAC_A
Cuts at: 932 932
Size: 4040
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstEII G'GTnAC_C
Cuts at: 333 333
Size: 4040
BstYI r'GATC_y
Cuts at: 1 1065 1532 1555 1890 2136 3148 3234 3246
Size: 1064 467 23 335 246 1012 86 12
Cuts at: 3246 3592 1
Size: 346 449
Fragments arranged by size:
1064 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 520 520
Size: 4040
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 923 947 1283 923
Size: 24 336 3680
Fragments arranged by size:
3680 336 24
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 353 479 629 1625 1799 2190 2217 3562 353
Size: 126 150 996 174 391 27 1345 831
Fragments arranged by size:
1345 996 831 391 174 150 126 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 202 1018 2063 2273 202
Size: 816 1045 210 1969
Fragments arranged by size:
1969 1045 816 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 874 2248 2558 2704 3850 874
Size: 1374 310 146 1146 1064
Fragments arranged by size:
1374 1146 1064 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1865 2297 3044 3312 682
Size: 1183 432 747 268 1410
Fragments arranged by size:
1410 1183 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1179 1179
Size: 4040
EcoRI G'AATT_C
Cuts at: 139 1044 139
Size: 905 3135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1882 1912 1914 1944 1882
Size: 30 2 30 3978
Fragments arranged by size:
3978 30 30 2
HincII GTy'rAC
Cuts at: 315 1079 315
Size: 764 3276
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 355 481 631 1801 355
Size: 126 150 1170 2594
Fragments arranged by size:
2594 1170 150 126
MslI CAynn'nnrTG
Cuts at: 689 738 928 940 2029 2156 689
Size: 49 190 12 1089 127 2573
Fragments arranged by size:
2573 1089 190 127 49 12
MspA1I CmG'CkG
Cuts at: 1151 1465 1825 2838 3083 1151
Size: 314 360 1013 245 2108
Fragments arranged by size:
2108 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NdeI CA'TA_TG
Cuts at: 465 465
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NsiI A_TGCA'T
Cuts at: 745 745
Size: 4040
NspI r_CATG'y
Cuts at: 1093 1593 2500 1093
Size: 500 907 2633
Fragments arranged by size:
2633 907 500
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 366 627 366
Size: 261 3779
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PstI C_TGCA'G
Cuts at: 1087 1772 1087
Size: 685 3355
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 1465 1825 1465
Size: 360 3680
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 969 1083 1768 2761 2952 33
Size: 936 114 685 993 191 1121
Fragments arranged by size:
1121 993 936 685 191 114
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 808 1195 2110 2602 2864 808
Size: 387 915 492 262 1984
Fragments arranged by size:
1984 915 492 387 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StuI AGG'CCT
Cuts at: 814 814
Size: 4040
StyI C'CwwG_G
Cuts at: 308 425 708 797 1174 1528 308
Size: 117 283 89 377 354 2820
Fragments arranged by size:
2820 377 354 283 117 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 543 1623 1756 2215 2424 543
Size: 1080 133 459 209 2159
Fragments arranged by size:
2159 1080 459 209 133
TatI w'GTAC_w
Cuts at: 932 3555 932
Size: 2623 1417
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 363 1535 306
Size: 57 1172 2811
Fragments arranged by size:
2811 1172 57
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
XmnI GAAnn'nnTTC
Cuts at: 1012 1012
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NsiI
NspI PciI PflMI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
PacI PasI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI