Restriction map for
pF1KE0878
(Circular) (Six-base) MAPSORT of: pF1KE0878.seq Check: 8521 from: 1 to: 4034
pF1KE0878 4034 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1072 1072
Size: 4034
Acc65I G'GTAC_C
Cuts at: 1050 1050
Size: 4034
AclI AA'CG_TT
Cuts at: 3874 3874
Size: 4034
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1859 2291 3038 3306 1859
Size: 432 747 268 2587
Fragments arranged by size:
2587 747 432 268
AflIII A'CryG_T
Cuts at: 1026 1386 2490 3467 1026
Size: 360 1104 977 1593
Fragments arranged by size:
1593 1104 977 360
AgeI A'CCGG_T
Cuts at: 2422 2422
Size: 4034
AleI CACnn'nnGTG
Cuts at: 838 838
Size: 4034
AlwNI CAG_nnn'CTG
Cuts at: 1339 2906 1339
Size: 1567 2467
ApaLI G'TGCA_C
Cuts at: 571 2804 571
Size: 2233 1801
ApoI r'AATT_y
Cuts at: 1038 3387 3643 1038
Size: 2349 256 1429
Fragments arranged by size:
2349 1429 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4034
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
AvaI C'yCGr_G
Cuts at: 1054 1054
Size: 4034
BamHI G'GATC_C
Cuts at: 1059 1059
Size: 4034
BanI G'GyrC_C
Cuts at: 1050 1464 1712 1747 1050
Size: 414 248 35 3337
Fragments arranged by size:
3337 414 248 35
BanII G_rGCy'C
Cuts at: 1048 2078 1048
Size: 1030 3004
BbeI G_GCGC'C
Cuts at: 1468 1716 1468
Size: 248 3786
BciVI GTATCCnnnnn_n'
Cuts at: 790 1932 2693 4034 790
Size: 1142 761 1341 790
Fragments arranged by size:
1341 1142 790 761
BclI T'GATC_A
Cuts at: 1554 1554
Size: 4034
BglII A'GATC_T
Cuts at: 1549 3586 1549
Size: 2037 1997
BlpI GC'TnA_GC
Cuts at: 532 1146 532
Size: 614 3420
Bme1580I G_kGCm'C
Cuts at: 575 1659 1752 2808 575
Size: 1084 93 1056 1801
Fragments arranged by size:
1801 1084 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1246 1327 1662 3806 1246
Size: 81 335 2144 1474
Fragments arranged by size:
2144 1474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4034
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 151
Size: 4034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1210 2089 2581 2879 1210
Size: 879 492 298 2365
Fragments arranged by size:
2365 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 422 3738 422
Size: 3316 718
BsaAI yAC'GTr
Cuts at: 1387 1387
Size: 4034
BsaBI GATnn'nnATC
Cuts at: 6 1548 3585 6
Size: 1542 2037 455
Fragments arranged by size:
2037 1542 455
BsaHI Gr'CG_yC
Cuts at: 1465 1713 2415 3911 1465
Size: 248 702 1496 1588
Fragments arranged by size:
1588 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1223 1744 2422 2696 2843 3254 1223
Size: 521 678 274 147 411 2003
Fragments arranged by size:
2003 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 342 395 1459 2217 2794 3290 342
Size: 53 1064 758 577 496 1086
Fragments arranged by size:
1086 1064 758 577 496 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 908 300
Size: 608 3426
BsiEI CG_ry'CG
Cuts at: 104 1576 1622 2830 104
Size: 1472 46 1208 1308
Fragments arranged by size:
1472 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 575 1048 1826 2016 2808 575
Size: 473 778 190 792 1801
Fragments arranged by size:
1801 792 778 473 190
Bsp1286I G_dGCh'C
Cuts at: 575 1048 1659 1752 1826 2016 2078 2808 575
Size: 473 611 93 74 190 62 730 1801
Fragments arranged by size:
1801 730 611 473 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3254 3254
Size: 4034
BspHI T'CATG_A
Cuts at: 587 3210 587
Size: 2623 1411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 748 1084 1298 1600 1981 748
Size: 336 214 302 381 2801
Fragments arranged by size:
2801 381 336 302 214
BsrBI CCG'CTC
Cuts at: 50 2326 2380 3864 4029 50
Size: 2276 54 1484 165 55
Fragments arranged by size:
2276 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 236 646 1946 236
Size: 410 1300 2324
Fragments arranged by size:
2324 1300 410
BsrFI r'CCGG_y
Cuts at: 2032 2213 2422 2032
Size: 181 209 3644
Fragments arranged by size:
3644 209 181
BsrGI T'GTAC_A
Cuts at: 965 965
Size: 4034
BssSI C'ACGA_G
Cuts at: 2305 2663 2305
Size: 358 3676
BstAPI GCAn_nnn'nTGC
Cuts at: 1411 1411
Size: 4034
BstBI TT'CG_AA
Cuts at: 2395 2395
Size: 4034
BstEII G'GTnAC_C
Cuts at: 840 840
Size: 4034
BstYI r'GATC_y
Cuts at: 1 1059 1526 1549 1884 2130 3142 3228 3240
Size: 1058 467 23 335 246 1012 86 12
Cuts at: 3240 3586 1
Size: 346 449
Fragments arranged by size:
1058 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 449 449
Size: 4034
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1984 2165 2328 1984
Size: 181 163 3690
Fragments arranged by size:
3690 181 163
BtsI GCAGTG_nn'
Cuts at: 1277 1277
Size: 4034
DraI TTT'AAA
Cuts at: 1033 1033
Size: 4034
DrdI GACnn_nn'nnGTC
Cuts at: 1740 2598 1740
Size: 858 3176
EaeI y'GGCC_r
Cuts at: 470 716 1619 1793 2184 2211 3556 470
Size: 246 903 174 391 27 1345 948
Fragments arranged by size:
1345 948 903 391 246 174 27
EagI C'GGCC_G
Cuts at: 1619 1619
Size: 4034
EarI CTCTTCn'nnn_
Cuts at: 406 491 2057 2267 406
Size: 85 1566 210 2173
Fragments arranged by size:
2173 1566 210 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 868 2242 2552 2698 3844 868
Size: 1374 310 146 1146 1058
Fragments arranged by size:
1374 1146 1058 310 146
EcoICRI GAG'CTC
Cuts at: 1046 1046
Size: 4034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 1859 2291 3038 3306 151
Size: 1708 432 747 268 879
Fragments arranged by size:
1708 879 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1173 1173
Size: 4034
EcoRI G'AATT_C
Cuts at: 1038 1038
Size: 4034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 4034
FspI TGC'GCA
Cuts at: 1815 1815
Size: 4034
HaeII r_GCGC'y
Cuts at: 1468 1716 2738 3717 1468
Size: 248 1022 979 1785
Fragments arranged by size:
1785 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1876 1906 1908 1938 1876
Size: 30 2 30 3972
Fragments arranged by size:
3972 30 30 2
HincII GTy'rAC
Cuts at: 596 1073 596
Size: 477 3557
KasI G'GCGC_C
Cuts at: 1464 1712 1464
Size: 248 3786
KpnI G_GTAC'C
Cuts at: 1054 1054
Size: 4034
MluI A'CGCG_T
Cuts at: 3467 3467
Size: 4034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2705 2889 2705
Size: 184 3850
MscI TGG'CCA
Cuts at: 472 718 1795 472
Size: 246 1077 2711
Fragments arranged by size:
2711 1077 246
MslI CAynn'nnrTG
Cuts at: 518 838 2023 2150 518
Size: 320 1185 127 2402
Fragments arranged by size:
2402 1185 320 127
MspA1I CmG'CkG
Cuts at: 1145 1459 1819 2832 3077 1145
Size: 314 360 1013 245 2102
Fragments arranged by size:
2102 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2215 2215
Size: 4034
NarI GG'CG_CC
Cuts at: 1465 1713 1465
Size: 248 3786
NcoI C'CATG_G
Cuts at: 449 449
Size: 4034
NdeI CA'TA_TG
Cuts at: 378 515 378
Size: 137 3897
NgoMIV G'CCGG_C
Cuts at: 2213 2213
Size: 4034
NspI r_CATG'y
Cuts at: 228 393 821 1087 1587 2494 228
Size: 165 428 266 500 907 1768
Fragments arranged by size:
1768 907 500 428 266 165
PciI A'CATG_T
Cuts at: 2490 2490
Size: 4034
PflMI CCAn_nnn'nTGG
Cuts at: 566 566
Size: 4034
PmeI GTTT'AAAC
Cuts at: 1033 1033
Size: 4034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3173 3205 3173
Size: 32 4002
PstI C_TGCA'G
Cuts at: 1081 1766 1081
Size: 685 3349
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4034
PvuII CAG'CTG
Cuts at: 1459 1819 1459
Size: 360 3674
RsrII CG'GwC_CG
Cuts at: 2229 2229
Size: 4034
SacI G_AGCT'C
Cuts at: 1048 1048
Size: 4034
SalI G'TCGA_C
Cuts at: 1071 1071
Size: 4034
SapI GCTCTTCn'nnn_
Cuts at: 406 2057 2267 406
Size: 1651 210 2173
Fragments arranged by size:
2173 1651 210
SbfI CC_TGCA'GG
Cuts at: 1081 1081
Size: 4034
ScaI AGT'ACT
Cuts at: 498 3551 498
Size: 3053 981
SfcI C'TryA_G
Cuts at: 33 663 1077 1762 2755 2946 33
Size: 630 414 685 993 191 1121
Fragments arranged by size:
1121 993 685 630 414 191
SfoI GGC'GCC
Cuts at: 1466 1714 1466
Size: 248 3786
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4034
SmaI CCC'GGG
Cuts at: 1056 1056
Size: 4034
SmlI C'TyrA_G
Cuts at: 1189 2104 2596 2858 1189
Size: 915 492 262 2365
Fragments arranged by size:
2365 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4034
SphI G_CATG'C
Cuts at: 1087 1587 1087
Size: 500 3534
StuI AGG'CCT
Cuts at: 808 808
Size: 4034
StyI C'CwwG_G
Cuts at: 449 1168 1522 449
Size: 719 354 2961
Fragments arranged by size:
2961 719 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 558 1617 1750 2209 2418 558
Size: 1059 133 459 209 2174
Fragments arranged by size:
2174 1059 459 209 133
TatI w'GTAC_w
Cuts at: 496 965 3549 496
Size: 469 2584 981
Fragments arranged by size:
2584 981 469
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4002
Tth111I GACn'n_nGTC
Cuts at: 1831 1831
Size: 4034
XbaI T'CTAG_A
Cuts at: 1065 1065
Size: 4034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1529 1529
Size: 4034
XmaI C'CCGG_G
Cuts at: 1054 1054
Size: 4034
XmnI GAAnn'nnTTC
Cuts at: 808 808
Size: 4034
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PflMI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI