Restriction map for
pF1KE0877
(Circular) (Six-base) MAPSORT of: pF1KE0877.seq Check: 1150 from: 1 to: 4070
pF1KE0877 4070 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 278 278
Size: 4070
AccI GT'mk_AC
Cuts at: 981 1108 981
Size: 127 3943
Acc65I G'GTAC_C
Cuts at: 1086 1086
Size: 4070
AclI AA'CG_TT
Cuts at: 3910 3910
Size: 4070
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1895 2327 3074 3342 907
Size: 988 432 747 268 1635
Fragments arranged by size:
1635 988 747 432 268
AflIII A'CryG_T
Cuts at: 1422 2526 3503 1422
Size: 1104 977 1989
Fragments arranged by size:
1989 1104 977
AgeI A'CCGG_T
Cuts at: 470 2458 470
Size: 1988 2082
AhdI GACnn_n'nnGTC
Cuts at: 563 563
Size: 4070
AleI CACnn'nnGTG
Cuts at: 406 406
Size: 4070
AlwNI CAG_nnn'CTG
Cuts at: 1375 2942 1375
Size: 1567 2503
ApaLI G'TGCA_C
Cuts at: 828 2840 828
Size: 2012 2058
ApoI r'AATT_y
Cuts at: 647 922 1074 3423 3679 647
Size: 275 152 2349 256 1038
Fragments arranged by size:
2349 1038 275 256 152
AseI AT'TA_AT
Cuts at: 20 20
Size: 4070
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
AvaI C'yCGr_G
Cuts at: 1090 1090
Size: 4070
BamHI G'GATC_C
Cuts at: 1095 1095
Size: 4070
BanI G'GyrC_C
Cuts at: 1086 1500 1748 1783 1086
Size: 414 248 35 3373
Fragments arranged by size:
3373 414 248 35
BanII G_rGCy'C
Cuts at: 433 1084 2114 433
Size: 651 1030 2389
Fragments arranged by size:
2389 1030 651
BbeI G_GCGC'C
Cuts at: 1504 1752 1504
Size: 248 3822
BbvCI CC'TCA_GC
Cuts at: 295 295
Size: 4070
BciVI GTATCCnnnnn_n'
Cuts at: 790 1968 2729 4070 790
Size: 1178 761 1341 790
Fragments arranged by size:
1341 1178 790 761
BclI T'GATC_A
Cuts at: 215 710 1590 215
Size: 495 880 2695
Fragments arranged by size:
2695 880 495
BfrBI ATG'CAT
Cuts at: 763 763
Size: 4070
BglII A'GATC_T
Cuts at: 1585 3622 1585
Size: 2037 2033
BlpI GC'TnA_GC
Cuts at: 1182 1182
Size: 4070
Bme1580I G_kGCm'C
Cuts at: 832 1695 1788 2844 832
Size: 863 93 1056 2058
Fragments arranged by size:
2058 1056 863 93
BmrI ACTGGGnnnn_n'
Cuts at: 1282 1363 1698 3842 1282
Size: 81 335 2144 1510
Fragments arranged by size:
2144 1510 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4070
Bpu10I CC'TnA_GC
Cuts at: 295 295
Size: 4070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 2125 2617 2915 1246
Size: 879 492 298 2401
Fragments arranged by size:
2401 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3774 3774
Size: 4070
BsaAI yAC'GTr
Cuts at: 1423 1423
Size: 4070
BsaBI GATnn'nnATC
Cuts at: 6 247 1584 3621 6
Size: 241 1337 2037 455
Fragments arranged by size:
2037 1337 455 241
BsaHI Gr'CG_yC
Cuts at: 1501 1749 2451 3947 1501
Size: 248 702 1496 1624
Fragments arranged by size:
1624 1496 702 248
BsaWI w'CCGG_w
Cuts at: 470 1259 1780 2458 2732 2879 3290 470
Size: 789 521 678 274 147 411 1250
Fragments arranged by size:
1250 789 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 513 543 710 740 513
Size: 30 167 30 3843
Fragments arranged by size:
3843 167 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 318 318
Size: 4070
BseYI C'CCAG_C
Cuts at: 225 1495 2253 2830 3326 225
Size: 1270 758 577 496 969
Fragments arranged by size:
1270 969 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 553 712 256
Size: 297 159 3614
Fragments arranged by size:
3614 297 159
BsiEI CG_ry'CG
Cuts at: 104 1612 1658 2866 104
Size: 1508 46 1208 1308
Fragments arranged by size:
1508 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 449 832 1084 1862 2052 2844 449
Size: 383 252 778 190 792 1675
Fragments arranged by size:
1675 792 778 383 252 190
Bsp1286I G_dGCh'C
Cuts at: 433 449 832 1084 1695 1788 1862 2052 2114
Size: 16 383 252 611 93 74 190 62
Cuts at: 2114 2844 433
Size: 730 1659
Fragments arranged by size:
1659 730 611 383 252 190 93 74
62 16
BspEI T'CCGG_A
Cuts at: 3290 3290
Size: 4070
BspHI T'CATG_A
Cuts at: 512 3246 512
Size: 2734 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 278 1120 1334 1636 2017 278
Size: 842 214 302 381 2331
Fragments arranged by size:
2331 842 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2362 2416 3900 4065 50
Size: 2312 54 1484 165 55
Fragments arranged by size:
2312 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1982 1982
Size: 4070
BsrFI r'CCGG_y
Cuts at: 470 2068 2249 2458 470
Size: 1598 181 209 2082
Fragments arranged by size:
2082 1598 209 181
BssSI C'ACGA_G
Cuts at: 2341 2699 2341
Size: 358 3712
BstAPI GCAn_nnn'nTGC
Cuts at: 1447 1447
Size: 4070
BstBI TT'CG_AA
Cuts at: 2431 2431
Size: 4070
BstYI r'GATC_y
Cuts at: 1 1095 1562 1585 1920 2166 3178 3264 3276
Size: 1094 467 23 335 246 1012 86 12
Cuts at: 3276 3622 1
Size: 346 449
Fragments arranged by size:
1094 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 425 575 425
Size: 150 3920
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2020 2201 2364 2020
Size: 181 163 3726
Fragments arranged by size:
3726 181 163
BtsI GCAGTG_nn'
Cuts at: 569 1313 569
Size: 744 3326
DraI TTT'AAA
Cuts at: 1069 1069
Size: 4070
DraIII CAC_nnn'GTG
Cuts at: 440 440
Size: 4070
DrdI GACnn_nn'nnGTC
Cuts at: 1776 2634 1776
Size: 858 3212
EaeI y'GGCC_r
Cuts at: 479 653 1655 1829 2220 2247 3592 479
Size: 174 1002 174 391 27 1345 957
Fragments arranged by size:
1345 1002 957 391 174 174 27
EagI C'GGCC_G
Cuts at: 1655 1655
Size: 4070
EarI CTCTTCn'nnn_
Cuts at: 295 336 2093 2303 295
Size: 41 1757 210 2062
Fragments arranged by size:
2062 1757 210 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2278 2588 2734 3880 2278
Size: 310 146 1146 2468
Fragments arranged by size:
2468 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1082 1082
Size: 4070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 1895 2327 3074 3342 907
Size: 988 432 747 268 1635
Fragments arranged by size:
1635 988 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1209 1209
Size: 4070
EcoRI G'AATT_C
Cuts at: 1074 1074
Size: 4070
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 4070
FspI TGC'GCA
Cuts at: 1851 1851
Size: 4070
HaeII r_GCGC'y
Cuts at: 1504 1752 2774 3753 1504
Size: 248 1022 979 1821
Fragments arranged by size:
1821 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 549 581 1014 1046 1912 1942 1944
Size: 32 26 32 433 32 866 30 2
Cuts at: 1944 1974 491
Size: 30 2587
Fragments arranged by size:
2587 866 433 32 32 32 30 30
26 2
HincII GTy'rAC
Cuts at: 1109 1109
Size: 4070
KasI G'GCGC_C
Cuts at: 1500 1748 1500
Size: 248 3822
KpnI G_GTAC'C
Cuts at: 1090 1090
Size: 4070
MluI A'CGCG_T
Cuts at: 3503 3503
Size: 4070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2741 2925 2741
Size: 184 3886
MscI TGG'CCA
Cuts at: 481 655 1831 481
Size: 174 1176 2720
Fragments arranged by size:
2720 1176 174
MslI CAynn'nnrTG
Cuts at: 280 406 574 706 736 2059 2186 280
Size: 126 168 132 30 1323 127 2164
Fragments arranged by size:
2164 1323 168 132 127 126 30
MspA1I CmG'CkG
Cuts at: 544 898 1181 1495 1855 2868 3113 544
Size: 354 283 314 360 1013 245 1501
Fragments arranged by size:
1501 1013 360 354 314 283 245
NaeI GCC'GGC
Cuts at: 2251 2251
Size: 4070
NarI GG'CG_CC
Cuts at: 1501 1749 1501
Size: 248 3822
NcoI C'CATG_G
Cuts at: 425 575 425
Size: 150 3920
NdeI CA'TA_TG
Cuts at: 465 465
Size: 4070
NgoMIV G'CCGG_C
Cuts at: 2249 2249
Size: 4070
NsiI A_TGCA'T
Cuts at: 765 765
Size: 4070
NspI r_CATG'y
Cuts at: 1123 1623 2530 1123
Size: 500 907 2663
Fragments arranged by size:
2663 907 500
PciI A'CATG_T
Cuts at: 2526 2526
Size: 4070
PflMI CCAn_nnn'nTGG
Cuts at: 843 843
Size: 4070
PfoI T'CCnGG_A
Cuts at: 550 550
Size: 4070
PmeI GTTT'AAAC
Cuts at: 1069 1069
Size: 4070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 3209
Size: 32 4038
PstI C_TGCA'G
Cuts at: 1117 1802 1117
Size: 685 3385
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4070
PvuII CAG'CTG
Cuts at: 544 1495 1855 544
Size: 951 360 2759
Fragments arranged by size:
2759 951 360
RsrII CG'GwC_CG
Cuts at: 2265 2265
Size: 4070
SacI G_AGCT'C
Cuts at: 1084 1084
Size: 4070
SalI G'TCGA_C
Cuts at: 1107 1107
Size: 4070
SapI GCTCTTCn'nnn_
Cuts at: 2093 2303 2093
Size: 210 3860
SbfI CC_TGCA'GG
Cuts at: 1117 1117
Size: 4070
ScaI AGT'ACT
Cuts at: 3587 3587
Size: 4070
SfcI C'TryA_G
Cuts at: 33 899 975 1113 1798 2791 2982 33
Size: 866 76 138 685 993 191 1121
Fragments arranged by size:
1121 993 866 685 191 138 76
SfoI GGC'GCC
Cuts at: 1502 1750 1502
Size: 248 3822
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
SmaI CCC'GGG
Cuts at: 1092 1092
Size: 4070
SmlI C'TyrA_G
Cuts at: 1225 2140 2632 2894 1225
Size: 915 492 262 2401
Fragments arranged by size:
2401 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4070
SphI G_CATG'C
Cuts at: 1123 1623 1123
Size: 500 3570
StyI C'CwwG_G
Cuts at: 425 575 1204 1558 425
Size: 150 629 354 2937
Fragments arranged by size:
2937 629 354 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1653 1786 2245 2454 1653
Size: 133 459 209 3269
Fragments arranged by size:
3269 459 209 133
TatI w'GTAC_w
Cuts at: 717 3585 717
Size: 2868 1202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 513 545 3587 3619 513
Size: 32 3042 32 964
Fragments arranged by size:
3042 964 32 32
Tth111I GACn'n_nGTC
Cuts at: 1867 1867
Size: 4070
XbaI T'CTAG_A
Cuts at: 258 1101 258
Size: 843 3227
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 663 1565 663
Size: 902 3168
XmaI C'CCGG_G
Cuts at: 1090 1090
Size: 4070
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BaeI BbsI BcgI BglI BmgBI BmtI BpmI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI