Restriction map for
pF1KE0874
(Circular) (Six-base) MAPSORT of: pF1KE0874.seq Check: 9677 from: 1 to: 4067
pF1KE0874 4067 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1105 1105
Size: 4067
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 1892 2324 3071 3339 169
Size: 1723 432 747 268 897
Fragments arranged by size:
1723 897 747 432 268
AflIII A'CryG_T
Cuts at: 761 1419 2523 3500 761
Size: 658 1104 977 1328
Fragments arranged by size:
1328 1104 977 658
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AleI CACnn'nnGTG
Cuts at: 352 352
Size: 4067
AlwNI CAG_nnn'CTG
Cuts at: 1372 2939 1372
Size: 1567 2500
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 139 647 1071 3420 3676 139
Size: 508 424 2349 256 530
Fragments arranged by size:
2349 530 508 424 256
AseI AT'TA_AT
Cuts at: 20 745 20
Size: 725 3342
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4067
BanI G'GyrC_C
Cuts at: 1083 1497 1745 1780 1083
Size: 414 248 35 3370
Fragments arranged by size:
3370 414 248 35
BanII G_rGCy'C
Cuts at: 1081 2111 1081
Size: 1030 3037
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 1587 1587
Size: 4067
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 1692 1785 2841 1692
Size: 93 1056 2918
Fragments arranged by size:
2918 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1279 1360 1695 3839 1279
Size: 81 335 2144 1507
Fragments arranged by size:
2144 1507 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 1243 2122 2614 2912 196
Size: 1047 879 492 298 1351
Fragments arranged by size:
1351 1047 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3771 3771
Size: 4067
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 1581 3618 6
Size: 1575 2037 455
Fragments arranged by size:
2037 1575 455
BsaHI Gr'CG_yC
Cuts at: 1498 1746 2448 3944 1498
Size: 248 702 1496 1621
Fragments arranged by size:
1621 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2455 2729 2876 3287 1256
Size: 521 678 274 147 411 2036
Fragments arranged by size:
2036 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 544 1492 2250 2827 3323 544
Size: 948 758 577 496 1288
Fragments arranged by size:
1288 948 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 424 419
Size: 5 4062
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 197 1081 1859 2049 2841 197
Size: 884 778 190 792 1423
Fragments arranged by size:
1423 884 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 197 1081 1692 1785 1859 2049 2111 2841 197
Size: 884 611 93 74 190 62 730 1423
Fragments arranged by size:
1423 884 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 512 659 3243 512
Size: 147 2584 1336
Fragments arranged by size:
2584 1336 147
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1117 1331 1633 2014 1117
Size: 214 302 381 3170
Fragments arranged by size:
3170 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1979 1979
Size: 4067
BsrFI r'CCGG_y
Cuts at: 2065 2246 2455 2065
Size: 181 209 3677
Fragments arranged by size:
3677 209 181
BsrGI T'GTAC_A
Cuts at: 881 881
Size: 4067
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstEII G'GTnAC_C
Cuts at: 163 163
Size: 4067
BstXI CCAn_nnnn'nTGG
Cuts at: 135 135
Size: 4067
BstYI r'GATC_y
Cuts at: 1 1092 1559 1582 1917 2163 3175 3261 3273
Size: 1091 467 23 335 246 1012 86 12
Cuts at: 3273 3619 1
Size: 346 449
Fragments arranged by size:
1091 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 425 536 107
Size: 318 111 3638
Fragments arranged by size:
3638 318 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 407 1310 407
Size: 903 3164
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 161 1773 2631 161
Size: 1612 858 1597
Fragments arranged by size:
1612 1597 858
EaeI y'GGCC_r
Cuts at: 422 710 1652 1826 2217 2244 3589 422
Size: 288 942 174 391 27 1345 900
Fragments arranged by size:
1345 942 900 391 288 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 801 880 2090 2300 801
Size: 79 1210 210 2568
Fragments arranged by size:
2568 1210 210 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2585 2731 3877 2275
Size: 310 146 1146 2465
Fragments arranged by size:
2465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 1892 2324 3071 3339 169
Size: 1723 432 747 268 897
Fragments arranged by size:
1723 897 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 548 1206 548
Size: 658 3409
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4067
EcoRV GAT'ATC
Cuts at: 502 502
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 833 925 2086 833
Size: 92 1161 2814
Fragments arranged by size:
2814 1161 92
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 1501 1749 2771 3750 1501
Size: 248 1022 979 1818
Fragments arranged by size:
1818 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 1909 1939 1941 1971 491
Size: 32 1386 30 2 30 2587
Fragments arranged by size:
2587 1386 32 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
HindIII A'AGCT_T
Cuts at: 815 815
Size: 4067
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MfeI C'AATT_G
Cuts at: 601 601
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2738 2922 2738
Size: 184 3883
MscI TGG'CCA
Cuts at: 424 712 1828 424
Size: 288 1116 2663
Fragments arranged by size:
2663 1116 288
MslI CAynn'nnrTG
Cuts at: 133 280 352 511 706 883 2056 2183 133
Size: 147 72 159 195 177 1173 127 2017
Fragments arranged by size:
2017 1173 195 177 159 147 127 72
MspA1I CmG'CkG
Cuts at: 544 1178 1492 1852 2865 3110 544
Size: 634 314 360 1013 245 1501
Fragments arranged by size:
1501 1013 634 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NcoI C'CATG_G
Cuts at: 107 425 536 107
Size: 318 111 3638
Fragments arranged by size:
3638 318 111
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NspI r_CATG'y
Cuts at: 1120 1620 2527 1120
Size: 500 907 2660
Fragments arranged by size:
2660 907 500
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4067
PfoI T'CCnGG_A
Cuts at: 550 550
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4035
PpuMI rG'GwC_Cy
Cuts at: 548 548
Size: 4067
PshAI GACnn'nnGTC
Cuts at: 847 847
Size: 4067
PstI C_TGCA'G
Cuts at: 579 616 1114 1799 579
Size: 37 498 685 2847
Fragments arranged by size:
2847 685 498 37
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 544 1492 1852 544
Size: 948 360 2759
Fragments arranged by size:
2759 948 360
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 801 2090 2300 801
Size: 1289 210 2568
Fragments arranged by size:
2568 1289 210
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 575 612 993 1110 1795 2788 2979 33
Size: 542 37 381 117 685 993 191 1121
Fragments arranged by size:
1121 993 685 542 381 191 117 37
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 175 1222 2137 2629 2891 175
Size: 1047 915 492 262 1351
Fragments arranged by size:
1351 1047 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
SspI AAT'ATT
Cuts at: 857 857
Size: 4067
StyI C'CwwG_G
Cuts at: 107 425 536 1201 1555 107
Size: 318 111 665 354 2619
Fragments arranged by size:
2619 665 354 318 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 182 1650 1783 2242 2451 182
Size: 1468 133 459 209 1798
Fragments arranged by size:
1798 1468 459 209 133
TatI w'GTAC_w
Cuts at: 284 579 881 3582 284
Size: 295 302 2701 769
Fragments arranged by size:
2701 769 302 295
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 324 356 3584 3616 324
Size: 32 3228 32 775
Fragments arranged by size:
3228 775 32 32
Tth111I GACn'n_nGTC
Cuts at: 1864 1864
Size: 4067
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1562 1562
Size: 4067
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
XmnI GAAnn'nnTTC
Cuts at: 143 143
Size: 4067
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI