Restriction map for
pF1KE0871
(Circular) (Six-base) MAPSORT of: pF1KE0871.seq Check: 9731 from: 1 to: 4085
pF1KE0871 4085 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 125 1123 125
Size: 998 3087
Acc65I G'GTAC_C
Cuts at: 1101 1101
Size: 4085
AclI AA'CG_TT
Cuts at: 3925 3925
Size: 4085
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1910 2342 3089 3357 1910
Size: 432 747 268 2638
Fragments arranged by size:
2638 747 432 268
AflII C'TTAA_G
Cuts at: 129 129
Size: 4085
AflIII A'CryG_T
Cuts at: 1437 2541 3518 1437
Size: 1104 977 2004
Fragments arranged by size:
2004 1104 977
AgeI A'CCGG_T
Cuts at: 2473 2473
Size: 4085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 303 551 303
Size: 248 3837
AlwNI CAG_nnn'CTG
Cuts at: 1390 2957 1390
Size: 1567 2518
ApaLI G'TGCA_C
Cuts at: 2855 2855
Size: 4085
ApoI r'AATT_y
Cuts at: 330 1064 1074 1089 3438 3694 330
Size: 734 10 15 2349 256 721
Fragments arranged by size:
2349 734 721 256 15 10
AseI AT'TA_AT
Cuts at: 20 20
Size: 4085
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
AvaI C'yCGr_G
Cuts at: 1105 1105
Size: 4085
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 514 547 514
Size: 33 4052
BamHI G'GATC_C
Cuts at: 932 1110 932
Size: 178 3907
BanI G'GyrC_C
Cuts at: 1101 1515 1763 1798 1101
Size: 414 248 35 3388
Fragments arranged by size:
3388 414 248 35
BanII G_rGCy'C
Cuts at: 748 1099 2129 748
Size: 351 1030 2704
Fragments arranged by size:
2704 1030 351
BbeI G_GCGC'C
Cuts at: 1519 1767 1519
Size: 248 3837
BciVI GTATCCnnnnn_n'
Cuts at: 595 1983 2744 4085 595
Size: 1388 761 1341 595
Fragments arranged by size:
1388 1341 761 595
BclI T'GATC_A
Cuts at: 660 1605 660
Size: 945 3140
BglII A'GATC_T
Cuts at: 347 1600 3637 347
Size: 1253 2037 795
Fragments arranged by size:
2037 1253 795
BlpI GC'TnA_GC
Cuts at: 1197 1197
Size: 4085
Bme1580I G_kGCm'C
Cuts at: 708 1710 1803 2859 708
Size: 1002 93 1056 1934
Fragments arranged by size:
1934 1056 1002 93
BmrI ACTGGGnnnn_n'
Cuts at: 466 1297 1378 1713 3857 466
Size: 831 81 335 2144 694
Fragments arranged by size:
2144 831 694 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 4085
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 1261 2140 2632 2930 364
Size: 897 879 492 298 1519
Fragments arranged by size:
1519 897 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3789 3789
Size: 4085
BsaAI yAC'GTr
Cuts at: 1438 1438
Size: 4085
BsaBI GATnn'nnATC
Cuts at: 6 1599 3636 6
Size: 1593 2037 455
Fragments arranged by size:
2037 1593 455
BsaHI Gr'CG_yC
Cuts at: 1516 1764 2466 3962 1516
Size: 248 702 1496 1639
Fragments arranged by size:
1639 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1274 1795 2473 2747 2894 3305 1274
Size: 521 678 274 147 411 2054
Fragments arranged by size:
2054 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 379 409 379
Size: 30 4055
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 974 974
Size: 4085
BseYI C'CCAG_C
Cuts at: 255 324 708 1510 2268 2845 3341 255
Size: 69 384 802 758 577 496 999
Fragments arranged by size:
999 802 758 577 496 384 69
BsiEI CG_ry'CG
Cuts at: 104 1627 1673 2881 104
Size: 1523 46 1208 1308
Fragments arranged by size:
1523 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 748 1099 1877 2067 2859 748
Size: 351 778 190 792 1974
Fragments arranged by size:
1974 792 778 351 190
Bsp1286I G_dGCh'C
Cuts at: 708 748 1099 1710 1803 1877 2067 2129 2859
Size: 40 351 611 93 74 190 62 730
Cuts at: 2859 708
Size: 1934
Fragments arranged by size:
1934 730 611 351 190 93 74 62
40
BspEI T'CCGG_A
Cuts at: 3305 3305
Size: 4085
BspHI T'CATG_A
Cuts at: 542 3261 542
Size: 2719 1366
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1135 1349 1651 2032 1135
Size: 214 302 381 3188
Fragments arranged by size:
3188 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2377 2431 3915 4080 50
Size: 2327 54 1484 165 55
Fragments arranged by size:
2327 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1997 1997
Size: 4085
BsrFI r'CCGG_y
Cuts at: 2083 2264 2473 2083
Size: 181 209 3695
Fragments arranged by size:
3695 209 181
BsrGI T'GTAC_A
Cuts at: 773 773
Size: 4085
BssSI C'ACGA_G
Cuts at: 2356 2714 2356
Size: 358 3727
BstAPI GCAn_nnn'nTGC
Cuts at: 1462 1462
Size: 4085
BstBI TT'CG_AA
Cuts at: 2446 2446
Size: 4085
BstYI r'GATC_y
Cuts at: 1 347 932 953 1110 1577 1600 1935 2181
Size: 346 585 21 157 467 23 335 246
Cuts at: 2181 3193 3279 3291 3637 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 585 467 449 346 346 335 246
157 86 23 21 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2035 2216 2379 2035
Size: 181 163 3741
Fragments arranged by size:
3741 181 163
BtsI GCAGTG_nn'
Cuts at: 287 523 1328 287
Size: 236 805 3044
Fragments arranged by size:
3044 805 236
ClaI AT'CG_AT
Cuts at: 501 501
Size: 4085
DraI TTT'AAA
Cuts at: 1084 1084
Size: 4085
DraIII CAC_nnn'GTG
Cuts at: 162 162
Size: 4085
DrdI GACnn_nn'nnGTC
Cuts at: 1791 2649 1791
Size: 858 3227
EaeI y'GGCC_r
Cuts at: 509 1670 1844 2235 2262 3607 509
Size: 1161 174 391 27 1345 987
Fragments arranged by size:
1345 1161 987 391 174 27
EagI C'GGCC_G
Cuts at: 1670 1670
Size: 4085
EarI CTCTTCn'nnn_
Cuts at: 223 1016 2108 2318 223
Size: 793 1092 210 1990
Fragments arranged by size:
1990 1092 793 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2293 2603 2749 3895 2293
Size: 310 146 1146 2483
Fragments arranged by size:
2483 1146 310 146
EcoICRI GAG'CTC
Cuts at: 746 1097 746
Size: 351 3734
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 418 1910 2342 3089 3357 418
Size: 1492 432 747 268 1146
Fragments arranged by size:
1492 1146 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 819 1224 819
Size: 405 3680
EcoRI G'AATT_C
Cuts at: 1064 1089 1064
Size: 25 4060
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2104 2104
Size: 4085
FspI TGC'GCA
Cuts at: 1866 1866
Size: 4085
HaeII r_GCGC'y
Cuts at: 1519 1767 2789 3768 1519
Size: 248 1022 979 1836
Fragments arranged by size:
1836 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 257 289 776 808 993 1025 1053 1085 1927
Size: 32 487 32 185 32 28 32 842
Cuts at: 1927 1957 1959 1989 257
Size: 30 2 30 2353
Fragments arranged by size:
2353 842 487 185 32 32 32 32
30 30 28 2
HincII GTy'rAC
Cuts at: 387 1124 387
Size: 737 3348
KasI G'GCGC_C
Cuts at: 1515 1763 1515
Size: 248 3837
KpnI G_GTAC'C
Cuts at: 1105 1105
Size: 4085
MluI A'CGCG_T
Cuts at: 3518 3518
Size: 4085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 817 2756 2940 817
Size: 1939 184 1962
Fragments arranged by size:
1962 1939 184
MscI TGG'CCA
Cuts at: 511 1846 511
Size: 1335 2750
MslI CAynn'nnrTG
Cuts at: 310 769 907 2074 2201 310
Size: 459 138 1167 127 2194
Fragments arranged by size:
2194 1167 459 138 127
MspA1I CmG'CkG
Cuts at: 808 832 1196 1510 1870 2883 3128 808
Size: 24 364 314 360 1013 245 1765
Fragments arranged by size:
1765 1013 364 360 314 245 24
NaeI GCC'GGC
Cuts at: 2266 2266
Size: 4085
NarI GG'CG_CC
Cuts at: 1516 1764 1516
Size: 248 3837
NdeI CA'TA_TG
Cuts at: 927 927
Size: 4085
NgoMIV G'CCGG_C
Cuts at: 2264 2264
Size: 4085
NspI r_CATG'y
Cuts at: 381 1138 1638 2545 381
Size: 757 500 907 1921
Fragments arranged by size:
1921 907 757 500
PciI A'CATG_T
Cuts at: 2541 2541
Size: 4085
PmeI GTTT'AAAC
Cuts at: 1084 1084
Size: 4085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3224 3256 3224
Size: 32 4053
PpuMI rG'GwC_Cy
Cuts at: 819 819
Size: 4085
PstI C_TGCA'G
Cuts at: 530 831 1132 1817 530
Size: 301 301 685 2798
Fragments arranged by size:
2798 685 301 301
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4085
PvuII CAG'CTG
Cuts at: 808 832 1510 1870 808
Size: 24 678 360 3023
Fragments arranged by size:
3023 678 360 24
RsrII CG'GwC_CG
Cuts at: 2280 2280
Size: 4085
SacI G_AGCT'C
Cuts at: 748 1099 748
Size: 351 3734
SalI G'TCGA_C
Cuts at: 1122 1122
Size: 4085
SapI GCTCTTCn'nnn_
Cuts at: 2108 2318 2108
Size: 210 3875
SbfI CC_TGCA'GG
Cuts at: 1132 1132
Size: 4085
ScaI AGT'ACT
Cuts at: 3602 3602
Size: 4085
SfcI C'TryA_G
Cuts at: 33 526 827 1128 1813 2806 2997 33
Size: 493 301 301 685 993 191 1121
Fragments arranged by size:
1121 993 685 493 301 301 191
SfoI GGC'GCC
Cuts at: 1517 1765 1517
Size: 248 3837
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4085
SmaI CCC'GGG
Cuts at: 1107 1107
Size: 4085
SmlI C'TyrA_G
Cuts at: 129 343 1240 2155 2647 2909 129
Size: 214 897 915 492 262 1305
Fragments arranged by size:
1305 915 897 492 262 214
SpeI A'CTAG_T
Cuts at: 67 766 67
Size: 699 3386
SphI G_CATG'C
Cuts at: 1138 1638 1138
Size: 500 3585
StyI C'CwwG_G
Cuts at: 404 597 1219 1573 404
Size: 193 622 354 2916
Fragments arranged by size:
2916 622 354 193
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 413 536 1668 1801 2260 2469 413
Size: 123 1132 133 459 209 2029
Fragments arranged by size:
2029 1132 459 209 133 123
TatI w'GTAC_w
Cuts at: 314 773 3600 314
Size: 459 2827 799
Fragments arranged by size:
2827 799 459
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3602 3634 3602
Size: 32 4053
Tth111I GACn'n_nGTC
Cuts at: 1882 1882
Size: 4085
XbaI T'CTAG_A
Cuts at: 1116 1116
Size: 4085
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1580 1580
Size: 4085
XmaI C'CCGG_G
Cuts at: 1105 1105
Size: 4085
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NspI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI