Restriction map for
pF1KE0870
(Circular) (Six-base) MAPSORT of: pF1KE0870.seq Check: 4055 from: 1 to: 4052
pF1KE0870 4052 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 1056 3405 3661 1056
Size: 2349 256 1447
Fragments arranged by size:
2349 1447 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 687 1072 687
Size: 385 3667
BamHI G'GATC_C
Cuts at: 788 1077 788
Size: 289 3763
BanI G'GyrC_C
Cuts at: 1068 1482 1730 1765 1068
Size: 414 248 35 3355
Fragments arranged by size:
3355 414 248 35
BanII G_rGCy'C
Cuts at: 1066 2096 1066
Size: 1030 3022
BbeI G_GCGC'C
Cuts at: 1486 1734 1486
Size: 248 3804
BbsI GAAGACnn'nnnn_
Cuts at: 138 228 138
Size: 90 3962
BbvCI CC'TCA_GC
Cuts at: 697 800 697
Size: 103 3949
BciVI GTATCCnnnnn_n'
Cuts at: 556 1950 2711 4052 556
Size: 1394 761 1341 556
Fragments arranged by size:
1394 1341 761 556
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglI GCCn_nnn'nGGC
Cuts at: 427 427
Size: 4052
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 661 1164 661
Size: 503 3549
Bme1580I G_kGCm'C
Cuts at: 534 1677 1770 2826 534
Size: 1143 93 1056 1760
Fragments arranged by size:
1760 1143 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 586 1264 1345 1680 3824 586
Size: 678 81 335 2144 814
Fragments arranged by size:
2144 814 678 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 153 144
Size: 9 4043
Bpu10I CC'TnA_GC
Cuts at: 697 800 697
Size: 103 3949
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 931 1228 2107 2599 2897 931
Size: 297 879 492 298 2086
Fragments arranged by size:
2086 879 492 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 310 738 3756 310
Size: 428 3018 606
Fragments arranged by size:
3018 606 428
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 266 706 1566 3603 6
Size: 260 440 860 2037 455
Fragments arranged by size:
2037 860 455 440 260
BsaHI Gr'CG_yC
Cuts at: 1483 1731 2433 3929 1483
Size: 248 702 1496 1606
Fragments arranged by size:
1606 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 112
Size: 30 4022
BseYI C'CCAG_C
Cuts at: 364 533 1035 1477 2235 2812 3308 364
Size: 169 502 442 758 577 496 1108
Fragments arranged by size:
1108 758 577 502 496 442 169
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 256
Size: 4052
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 492 1066 1844 2034 2826 492
Size: 574 778 190 792 1718
Fragments arranged by size:
1718 792 778 574 190
BsmI GAATG_Cn'
Cuts at: 353 1020 353
Size: 667 3385
Bsp1286I G_dGCh'C
Cuts at: 492 534 1066 1677 1770 1844 2034 2096 2826
Size: 42 532 611 93 74 190 62 730
Cuts at: 2826 492
Size: 1718
Fragments arranged by size:
1718 730 611 532 190 93 74 62
42
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 328 1102 1316 1618 1999 328
Size: 774 214 302 381 2381
Fragments arranged by size:
2381 774 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 237 1964 237
Size: 1727 2325
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 258 258
Size: 4052
BstYI r'GATC_y
Cuts at: 1 701 788 1077 1544 1567 1902 2148 3160
Size: 700 87 289 467 23 335 246 1012
Cuts at: 3160 3246 3258 3604 1
Size: 86 12 346 449
Fragments arranged by size:
1012 700 467 449 346 335 289 246
87 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 886 886
Size: 4052
BtgI C'CryG_G
Cuts at: 107 458 107
Size: 351 3701
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 1295 1295
Size: 4052
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 479 1637 1811 2202 2229 3574 479
Size: 1158 174 391 27 1345 957
Fragments arranged by size:
1345 1158 957 391 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 295 2075 2285 295
Size: 1780 210 2062
Fragments arranged by size:
2062 1780 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 153 1877 2309 3056 3324 144
Size: 9 1724 432 747 268 872
Fragments arranged by size:
1724 872 747 432 268 9
EcoNI CCTnn'n_nnAGG
Cuts at: 132 132
Size: 4052
EcoO109I rG'GnC_Cy
Cuts at: 1191 1191
Size: 4052
EcoRI G'AATT_C
Cuts at: 1056 1056
Size: 4052
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 834 2071 834
Size: 1237 2815
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 1486 1734 2756 3735 1486
Size: 248 1022 979 1803
Fragments arranged by size:
1803 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1894 1924 1926 1956 1894
Size: 30 2 30 3990
Fragments arranged by size:
3990 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
KasI G'GCGC_C
Cuts at: 1482 1730 1482
Size: 248 3804
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2723 2907 2723
Size: 184 3868
MscI TGG'CCA
Cuts at: 481 1813 481
Size: 1332 2720
MslI CAynn'nnrTG
Cuts at: 280 2041 2168 280
Size: 1761 127 2164
Fragments arranged by size:
2164 1761 127
MspA1I CmG'CkG
Cuts at: 537 1163 1477 1837 2850 3095 537
Size: 626 314 360 1013 245 1494
Fragments arranged by size:
1494 1013 626 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 1483 1731 1483
Size: 248 3804
NcoI C'CATG_G
Cuts at: 107 458 107
Size: 351 3701
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 1105 1605 2512 1105
Size: 500 907 2645
Fragments arranged by size:
2645 907 500
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PfoI T'CCnGG_A
Cuts at: 130 130
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PstI C_TGCA'G
Cuts at: 616 1099 1784 616
Size: 483 685 2884
Fragments arranged by size:
2884 685 483
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 537 1477 1837 537
Size: 940 360 2752
Fragments arranged by size:
2752 940 360
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 612 944 1095 1780 2773 2964 33
Size: 579 332 151 685 993 191 1121
Fragments arranged by size:
1121 993 685 579 332 191 151
SfoI GGC'GCC
Cuts at: 1484 1732 1484
Size: 248 3804
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 910 1207 2122 2614 2876 910
Size: 297 915 492 262 2086
Fragments arranged by size:
2086 915 492 297 262
SpeI A'CTAG_T
Cuts at: 67 588 67
Size: 521 3531
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StuI AGG'CCT
Cuts at: 817 817
Size: 4052
StyI C'CwwG_G
Cuts at: 107 458 1186 1540 107
Size: 351 728 354 2619
Fragments arranged by size:
2619 728 354 351
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1635 1768 2227 2436 1635
Size: 133 459 209 3251
Fragments arranged by size:
3251 459 209 133
TatI w'GTAC_w
Cuts at: 433 3567 433
Size: 3134 918
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1547 1547
Size: 4052
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
XmnI GAAnn'nnTTC
Cuts at: 817 1018 817
Size: 201 3851
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI