Restriction map for
pF1KE0868
(Circular) (Six-base) MAPSORT of: pF1KE0868.seq Check: 1404 from: 1 to: 4028
pF1KE0868 4028 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 808 808
Size: 4028
AccI GT'mk_AC
Cuts at: 1066 1066
Size: 4028
Acc65I G'GTAC_C
Cuts at: 1044 1044
Size: 4028
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1853 2285 3032 3300 1853
Size: 432 747 268 2581
Fragments arranged by size:
2581 747 432 268
AflIII A'CryG_T
Cuts at: 1380 2484 3461 1380
Size: 1104 977 1947
Fragments arranged by size:
1947 1104 977
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AleI CACnn'nnGTG
Cuts at: 304 820 304
Size: 516 3512
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 103 135 994 1026 103
Size: 32 859 32 3105
Fragments arranged by size:
3105 859 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1004 1333 2900 1004
Size: 329 1567 2132
Fragments arranged by size:
2132 1567 329
ApaLI G'TGCA_C
Cuts at: 2798 2798
Size: 4028
ApoI r'AATT_y
Cuts at: 112 1032 3381 3637 112
Size: 920 2349 256 503
Fragments arranged by size:
2349 920 503 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 1048 1048
Size: 4028
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 312 1044 1458 1706 1741 312
Size: 732 414 248 35 2599
Fragments arranged by size:
2599 732 414 248 35
BanII G_rGCy'C
Cuts at: 1042 2072 1042
Size: 1030 2998
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BbsI GAAGACnn'nnnn_
Cuts at: 355 355
Size: 4028
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 191 1548 191
Size: 1357 2671
BfrBI ATG'CAT
Cuts at: 636 636
Size: 4028
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 1140 1140
Size: 4028
Bme1580I G_kGCm'C
Cuts at: 317 865 1653 1746 2802 317
Size: 548 788 93 1056 1543
Fragments arranged by size:
1543 1056 788 548 93
BmrI ACTGGGnnnn_n'
Cuts at: 209 409 1240 1321 1656 3800 209
Size: 200 831 81 335 2144 437
Fragments arranged by size:
2144 831 437 335 200 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 855 855
Size: 4028
Bpu10I CC'TnA_GC
Cuts at: 593 593
Size: 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 742 1204 2083 2575 2873 742
Size: 462 879 492 298 1897
Fragments arranged by size:
1897 879 492 462 298
BsaI GGTCTCn'nnnn_
Cuts at: 3732 3732
Size: 4028
BsaAI yAC'GTr
Cuts at: 1381 1381
Size: 4028
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 1459 1707 2409 3905 1459
Size: 248 702 1496 1582
Fragments arranged by size:
1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1217 1738 2416 2690 2837 3248 1217
Size: 521 678 274 147 411 1997
Fragments arranged by size:
1997 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 472 472
Size: 4028
BseYI C'CCAG_C
Cuts at: 865 1453 2211 2788 3284 865
Size: 588 758 577 496 1609
Fragments arranged by size:
1609 758 588 577 496
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2824 104
Size: 1466 46 1208 1308
Fragments arranged by size:
1466 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 608 651 1042 1820 2010 2802 608
Size: 43 391 778 190 792 1834
Fragments arranged by size:
1834 792 778 391 190 43
Bsp1286I G_dGCh'C
Cuts at: 317 608 651 865 1042 1653 1746 1820 2010
Size: 291 43 214 177 611 93 74 190
Cuts at: 2010 2072 2802 317
Size: 62 730 1543
Fragments arranged by size:
1543 730 611 291 214 190 177 93
74 62 43
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 485 663 3204 485
Size: 178 2541 1309
Fragments arranged by size:
2541 1309 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 808 1078 1292 1594 1975 808
Size: 270 214 302 381 2861
Fragments arranged by size:
2861 381 302 270 214
BsrBI CCG'CTC
Cuts at: 50 2320 2374 3858 4023 50
Size: 2270 54 1484 165 55
Fragments arranged by size:
2270 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 392 698 1940 392
Size: 306 1242 2480
Fragments arranged by size:
2480 1242 306
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BsrGI T'GTAC_A
Cuts at: 911 911
Size: 4028
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 675 1405 675
Size: 730 3298
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstXI CCAn_nnnn'nTGG
Cuts at: 381 381
Size: 4028
BstYI r'GATC_y
Cuts at: 1 761 1053 1520 1543 1878 2124 3136 3222
Size: 760 292 467 23 335 246 1012 86
Cuts at: 3222 3234 3580 1
Size: 12 346 449
Fragments arranged by size:
1012 760 467 449 346 335 292 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 955 955
Size: 4028
BtgI C'CryG_G
Cuts at: 107 431 107
Size: 324 3704
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 299 311 411 928 1271 299
Size: 12 100 517 343 3056
Fragments arranged by size:
3056 517 343 100 12
DraI TTT'AAA
Cuts at: 1027 1027
Size: 4028
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 326 452 777 1613 1787 2178 2205 3550 326
Size: 126 325 836 174 391 27 1345 804
Fragments arranged by size:
1345 836 804 391 325 174 126 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 810 2051 2261 810
Size: 1241 210 2577
Fragments arranged by size:
2577 1241 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2546 2692 3838 2236
Size: 310 146 1146 2426
Fragments arranged by size:
2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 855 1853 2285 3032 3300 855
Size: 998 432 747 268 1583
Fragments arranged by size:
1583 998 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1167 1167
Size: 4028
EcoRI G'AATT_C
Cuts at: 1032 1032
Size: 4028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 4028
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 1462 1710 2732 3711 1462
Size: 248 1022 979 1779
Fragments arranged by size:
1779 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 342 374 480 512 1870 1900 1902 1932 342
Size: 32 106 32 1358 30 2 30 2438
Fragments arranged by size:
2438 1358 106 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 1048 1048
Size: 4028
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2699 2883 2699
Size: 184 3844
MscI TGG'CCA
Cuts at: 328 454 1789 328
Size: 126 1335 2567
Fragments arranged by size:
2567 1335 126
MslI CAynn'nnrTG
Cuts at: 217 253 304 379 662 703 820 2017 2144
Size: 36 51 75 283 41 117 1197 127
Cuts at: 2144 217
Size: 2101
Fragments arranged by size:
2101 1197 283 127 117 75 51 41
36
MspA1I CmG'CkG
Cuts at: 1139 1453 1813 2826 3071 1139
Size: 314 360 1013 245 2096
Fragments arranged by size:
2096 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 107 431 107
Size: 324 3704
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NsiI A_TGCA'T
Cuts at: 638 638
Size: 4028
NspI r_CATG'y
Cuts at: 640 1081 1581 2488 640
Size: 441 500 907 2180
Fragments arranged by size:
2180 907 500 441
PciI A'CATG_T
Cuts at: 2484 2484
Size: 4028
PflMI CCAn_nnn'nTGG
Cuts at: 324 324
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 974 1006 974
Size: 32 3996
PstI C_TGCA'G
Cuts at: 306 833 1075 1760 306
Size: 527 242 685 2574
Fragments arranged by size:
2574 685 527 242
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 1453 1813 1453
Size: 360 3668
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 810 2051 2261 810
Size: 1241 210 2577
Fragments arranged by size:
2577 1241 210
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 302 337 829 1071 1756 2749 2940 33
Size: 269 35 492 242 685 993 191 1121
Fragments arranged by size:
1121 993 685 492 269 242 191 35
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 1050 1050
Size: 4028
SmlI C'TyrA_G
Cuts at: 757 1183 2098 2590 2852 757
Size: 426 915 492 262 1933
Fragments arranged by size:
1933 915 492 426 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 1081 1581 1081
Size: 500 3528
StyI C'CwwG_G
Cuts at: 107 165 431 1162 1516 107
Size: 58 266 731 354 2619
Fragments arranged by size:
2619 731 354 266 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 391 1611 1744 2203 2412 391
Size: 1220 133 459 209 2007
Fragments arranged by size:
2007 1220 459 209 133
TatI w'GTAC_w
Cuts at: 257 911 3543 257
Size: 654 2632 742
Fragments arranged by size:
2632 742 654
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 3545
Size: 32 3996
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 360 772 1523 360
Size: 412 751 2865
Fragments arranged by size:
2865 751 412
XmaI C'CCGG_G
Cuts at: 1048 1048
Size: 4028
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PflMI PmeI
PpiI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI