Restriction map for
pF1KE0867
(Circular) (Six-base) MAPSORT of: pF1KE0867.seq Check: 4679 from: 1 to: 4040
pF1KE0867 4040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 2297 3044 3312 1865
Size: 432 747 268 2593
Fragments arranged by size:
2593 747 432 268
AflIII A'CryG_T
Cuts at: 687 1392 2496 3473 687
Size: 705 1104 977 1254
Fragments arranged by size:
1254 1104 977 705
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 183 1345 2912 183
Size: 1162 1567 1311
Fragments arranged by size:
1567 1311 1162
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 1030 1044 3393 3649 1030
Size: 14 2349 256 1421
Fragments arranged by size:
2349 1421 256 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
BamHI G'GATC_C
Cuts at: 791 1065 791
Size: 274 3766
BanI G'GyrC_C
Cuts at: 403 809 1056 1470 1718 1753 403
Size: 406 247 414 248 35 2690
Fragments arranged by size:
2690 414 406 248 247 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 813 1474 1722 813
Size: 661 248 3131
Fragments arranged by size:
3131 661 248
BbsI GAAGACnn'nnnn_
Cuts at: 189 417 759 189
Size: 228 342 3470
Fragments arranged by size:
3470 342 228
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 629 1555 3592 629
Size: 926 2037 1077
Fragments arranged by size:
2037 1077 926
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 1665 1758 2814 1665
Size: 93 1056 2891
Fragments arranged by size:
2891 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 890 1252 1333 1668 3812 890
Size: 362 81 335 2144 1118
Fragments arranged by size:
2144 1118 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
Bpu10I CC'TnA_GC
Cuts at: 730 730
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 754 1554 3591 6
Size: 748 800 2037 455
Fragments arranged by size:
2037 800 748 455
BsaHI Gr'CG_yC
Cuts at: 810 1471 1719 2421 3917 810
Size: 661 248 702 1496 933
Fragments arranged by size:
1496 933 702 661 248
BsaWI w'CCGG_w
Cuts at: 794 1229 1750 2428 2702 2849 3260 794
Size: 435 521 678 274 147 411 1574
Fragments arranged by size:
1574 678 521 435 411 274 147
BseYI C'CCAG_C
Cuts at: 561 1465 2223 2800 3296 561
Size: 904 758 577 496 1305
Fragments arranged by size:
1305 904 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 855 698
Size: 157 3883
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1054 1832 2022 2814 1054
Size: 778 190 792 2280
Fragments arranged by size:
2280 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1054 1665 1758 1832 2022 2084 2814 1054
Size: 611 93 74 190 62 730 2280
Fragments arranged by size:
2280 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 515 3216 515
Size: 2701 1339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1090 1304 1606 1987 838
Size: 252 214 302 381 2891
Fragments arranged by size:
2891 381 302 252 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1952 1952
Size: 4040
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 629 791 1065 1532 1555 1890 2136 3148
Size: 628 162 274 467 23 335 246 1012
Cuts at: 3148 3234 3246 3592 1
Size: 86 12 346 449
Fragments arranged by size:
1012 628 467 449 346 335 274 246
162 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 170 1012 170
Size: 842 3198
BtgI C'CryG_G
Cuts at: 107 107
Size: 4040
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 577 1283 577
Size: 706 3334
DraI TTT'AAA
Cuts at: 965 1039 965
Size: 74 3966
DrdI GACnn_nn'nnGTC
Cuts at: 172 1746 2604 172
Size: 1574 858 1608
Fragments arranged by size:
1608 1574 858
EaeI y'GGCC_r
Cuts at: 1625 1799 2190 2217 3562 1625
Size: 174 391 27 1345 2103
Fragments arranged by size:
2103 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 840 2063 2273 840
Size: 1223 210 2607
Fragments arranged by size:
2607 1223 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 2297 3044 3312 1865
Size: 432 747 268 2593
Fragments arranged by size:
2593 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1179 1179
Size: 4040
EcoRI G'AATT_C
Cuts at: 1044 1044
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 813 1474 1722 2744 3723 813
Size: 661 248 1022 979 1130
Fragments arranged by size:
1130 1022 979 661 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 195 227 494 526 1882 1912 1914 1944 195
Size: 32 267 32 1356 30 2 30 2291
Fragments arranged by size:
2291 1356 267 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 809 1470 1718 809
Size: 661 248 3131
Fragments arranged by size:
3131 661 248
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MfeI C'AATT_G
Cuts at: 239 239
Size: 4040
MluI A'CGCG_T
Cuts at: 687 3473 687
Size: 2786 1254
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 280 409 623 847 2029 2156 280
Size: 129 214 224 1182 127 2164
Fragments arranged by size:
2164 1182 224 214 129 127
MspA1I CmG'CkG
Cuts at: 1151 1465 1825 2838 3083 1151
Size: 314 360 1013 245 2108
Fragments arranged by size:
2108 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 810 1471 1719 810
Size: 661 248 3131
Fragments arranged by size:
3131 661 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4040
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 654 1093 1593 2500 654
Size: 439 500 907 2194
Fragments arranged by size:
2194 907 500 439
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PstI C_TGCA'G
Cuts at: 660 863 1087 1772 660
Size: 203 224 685 2928
Fragments arranged by size:
2928 685 224 203
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 1465 1825 1465
Size: 360 3680
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 840 2063 2273 840
Size: 1223 210 2607
Fragments arranged by size:
2607 1223 210
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 656 859 978 1083 1768 2761 2952 33
Size: 623 203 119 105 685 993 191 1121
Fragments arranged by size:
1121 993 685 623 203 191 119 105
SfoI GGC'GCC
Cuts at: 811 1472 1720 811
Size: 661 248 3131
Fragments arranged by size:
3131 661 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 1195 2110 2602 2864 1195
Size: 915 492 262 2371
Fragments arranged by size:
2371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
StyI C'CwwG_G
Cuts at: 107 350 554 1174 1528 107
Size: 243 204 620 354 2619
Fragments arranged by size:
2619 620 354 243 204
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 421 1623 1756 2215 2424 421
Size: 1202 133 459 209 2037
Fragments arranged by size:
2037 1202 459 209 133
TatI w'GTAC_w
Cuts at: 284 3555 284
Size: 3271 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 312 1071 312
Size: 759 3281
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 342 1535 342
Size: 1193 2847
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI