Restriction map for
pF1KE0864
(Circular) (Six-base) MAPSORT of: pF1KE0864.seq Check: 9934 from: 1 to: 4070
pF1KE0864 4070 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 850 850
Size: 4070
AccI GT'mk_AC
Cuts at: 953 1108 953
Size: 155 3915
Acc65I G'GTAC_C
Cuts at: 1086 1086
Size: 4070
AclI AA'CG_TT
Cuts at: 1064 3910 1064
Size: 2846 1224
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1895 2327 3074 3342 1895
Size: 432 747 268 2623
Fragments arranged by size:
2623 747 432 268
AflIII A'CryG_T
Cuts at: 776 1422 2526 3503 776
Size: 646 1104 977 1343
Fragments arranged by size:
1343 1104 977 646
AgeI A'CCGG_T
Cuts at: 2458 2458
Size: 4070
AleI CACnn'nnGTG
Cuts at: 343 343
Size: 4070
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 565 754 565
Size: 189 3881
AlwNI CAG_nnn'CTG
Cuts at: 181 552 1054 1375 2942 181
Size: 371 502 321 1567 1309
Fragments arranged by size:
1567 1309 502 371 321
ApaI G_GGCC'C
Cuts at: 384 417 564 384
Size: 33 147 3890
Fragments arranged by size:
3890 147 33
ApaLI G'TGCA_C
Cuts at: 267 540 703 2840 267
Size: 273 163 2137 1497
Fragments arranged by size:
2137 1497 273 163
ApoI r'AATT_y
Cuts at: 184 1074 3423 3679 184
Size: 890 2349 256 575
Fragments arranged by size:
2349 890 575 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4070
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
AvaI C'yCGr_G
Cuts at: 1090 1090
Size: 4070
BamHI G'GATC_C
Cuts at: 1095 1095
Size: 4070
BanI G'GyrC_C
Cuts at: 1086 1500 1748 1783 1086
Size: 414 248 35 3373
Fragments arranged by size:
3373 414 248 35
BanII G_rGCy'C
Cuts at: 384 417 564 1084 2114 384
Size: 33 147 520 1030 2340
Fragments arranged by size:
2340 1030 520 147 33
BbeI G_GCGC'C
Cuts at: 1504 1752 1504
Size: 248 3822
BbsI GAAGACnn'nnnn_
Cuts at: 120 120
Size: 4070
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4036
BciVI GTATCCnnnnn_n'
Cuts at: 1968 2729 4070 1968
Size: 761 1341 1968
Fragments arranged by size:
1968 1341 761
BclI T'GATC_A
Cuts at: 1590 1590
Size: 4070
BglII A'GATC_T
Cuts at: 1585 3622 1585
Size: 2037 2033
BlpI GC'TnA_GC
Cuts at: 1182 1182
Size: 4070
Bme1580I G_kGCm'C
Cuts at: 271 384 417 544 564 707 1695 1788 2844
Size: 113 33 127 20 143 988 93 1056
Cuts at: 2844 271
Size: 1497
Fragments arranged by size:
1497 1056 988 143 127 113 93 33
20
BmrI ACTGGGnnnn_n'
Cuts at: 1282 1363 1698 3842 1282
Size: 81 335 2144 1510
Fragments arranged by size:
2144 1510 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4070
Bpu10I CC'TnA_GC
Cuts at: 799 799
Size: 4070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1246 2125 2617 2915 1246
Size: 879 492 298 2401
Fragments arranged by size:
2401 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3774 3774
Size: 4070
BsaAI yAC'GTr
Cuts at: 777 1423 777
Size: 646 3424
BsaBI GATnn'nnATC
Cuts at: 6 1584 3621 6
Size: 1578 2037 455
Fragments arranged by size:
2037 1578 455
BsaHI Gr'CG_yC
Cuts at: 1058 1501 1749 2451 3947 1058
Size: 443 248 702 1496 1181
Fragments arranged by size:
1496 1181 702 443 248
BsaWI w'CCGG_w
Cuts at: 1259 1780 2458 2732 2879 3290 1259
Size: 521 678 274 147 411 2039
Fragments arranged by size:
2039 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 208 1041 205
Size: 3 833 3234
Fragments arranged by size:
3234 833 3
BseYI C'CCAG_C
Cuts at: 552 1495 2253 2830 3326 552
Size: 943 758 577 496 1296
Fragments arranged by size:
1296 943 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 493
Size: 4070
BsiEI CG_ry'CG
Cuts at: 104 1612 1658 2866 104
Size: 1508 46 1208 1308
Fragments arranged by size:
1508 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 271 544 707 1023 1084 1862 2052 2844 271
Size: 273 163 316 61 778 190 792 1497
Fragments arranged by size:
1497 792 778 316 273 190 163 61
BsmBI CGTCTCn'nnnn_
Cuts at: 774 774
Size: 4070
Bsp1286I G_dGCh'C
Cuts at: 271 384 417 544 564 707 1023 1084 1695
Size: 113 33 127 20 143 316 61 611
Cuts at: 1695 1788 1862 2052 2114 2844 271
Size: 93 74 190 62 730 1497
Fragments arranged by size:
1497 730 611 316 190 143 127 113
93 74 62 61 33 20
BspEI T'CCGG_A
Cuts at: 3290 3290
Size: 4070
BspHI T'CATG_A
Cuts at: 524 3246 524
Size: 2722 1348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 850 908 1120 1334 1636 2017 850
Size: 58 212 214 302 381 2903
Fragments arranged by size:
2903 381 302 214 212 58
BsrBI CCG'CTC
Cuts at: 50 2362 2416 3900 4065 50
Size: 2312 54 1484 165 55
Fragments arranged by size:
2312 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1982 1982
Size: 4070
BsrFI r'CCGG_y
Cuts at: 2068 2249 2458 2068
Size: 181 209 3680
Fragments arranged by size:
3680 209 181
BssSI C'ACGA_G
Cuts at: 736 892 2341 2699 736
Size: 156 1449 358 2107
Fragments arranged by size:
2107 1449 358 156
BstAPI GCAn_nnn'nTGC
Cuts at: 1447 1447
Size: 4070
BstBI TT'CG_AA
Cuts at: 2431 2431
Size: 4070
BstXI CCAn_nnnn'nTGG
Cuts at: 114 375 399 114
Size: 261 24 3785
Fragments arranged by size:
3785 261 24
BstYI r'GATC_y
Cuts at: 1 1095 1562 1585 1920 2166 3178 3264 3276
Size: 1094 467 23 335 246 1012 86 12
Cuts at: 3276 3622 1
Size: 346 449
Fragments arranged by size:
1094 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 997 997
Size: 4070
BtgI C'CryG_G
Cuts at: 344 860 344
Size: 516 3554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 674 2020 2201 2364 674
Size: 1346 181 163 2380
Fragments arranged by size:
2380 1346 181 163
BtsI GCAGTG_nn'
Cuts at: 1313 1313
Size: 4070
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 135 170 135
Size: 35 4035
DraI TTT'AAA
Cuts at: 1069 1069
Size: 4070
DrdI GACnn_nn'nnGTC
Cuts at: 1776 2634 1776
Size: 858 3212
EaeI y'GGCC_r
Cuts at: 627 1655 1829 2220 2247 3592 627
Size: 1028 174 391 27 1345 1105
Fragments arranged by size:
1345 1105 1028 391 174 27
EagI C'GGCC_G
Cuts at: 1655 1655
Size: 4070
EarI CTCTTCn'nnn_
Cuts at: 205 214 583 2093 2303 205
Size: 9 369 1510 210 1972
Fragments arranged by size:
1972 1510 369 210 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2278 2588 2734 3880 2278
Size: 310 146 1146 2468
Fragments arranged by size:
2468 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1082 1082
Size: 4070
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1895 2327 3074 3342 1895
Size: 432 747 268 2623
Fragments arranged by size:
2623 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 380 413 560 561 1209 380
Size: 33 147 1 648 3241
Fragments arranged by size:
3241 648 147 33 1
EcoRI G'AATT_C
Cuts at: 184 1074 184
Size: 890 3180
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2089 2089
Size: 4070
FspI TGC'GCA
Cuts at: 1851 1851
Size: 4070
HaeII r_GCGC'y
Cuts at: 1504 1752 2774 3753 1504
Size: 248 1022 979 1821
Fragments arranged by size:
1821 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1912 1942 1944 1974 1912
Size: 30 2 30 4008
Fragments arranged by size:
4008 30 30 2
HincII GTy'rAC
Cuts at: 1109 1109
Size: 4070
KasI G'GCGC_C
Cuts at: 1500 1748 1500
Size: 248 3822
KpnI G_GTAC'C
Cuts at: 1090 1090
Size: 4070
MluI A'CGCG_T
Cuts at: 3503 3503
Size: 4070
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 335 392 2741 2925 335
Size: 57 2349 184 1480
Fragments arranged by size:
2349 1480 184 57
MscI TGG'CCA
Cuts at: 1831 1831
Size: 4070
MslI CAynn'nnrTG
Cuts at: 112 259 292 343 547 700 736 2059 2186
Size: 147 33 51 204 153 36 1323 127
Cuts at: 2186 112
Size: 1996
Fragments arranged by size:
1996 1323 204 153 147 127 51 36
33
MspA1I CmG'CkG
Cuts at: 507 1181 1495 1855 2868 3113 507
Size: 674 314 360 1013 245 1464
Fragments arranged by size:
1464 1013 674 360 314 245
NaeI GCC'GGC
Cuts at: 2251 2251
Size: 4070
NarI GG'CG_CC
Cuts at: 1501 1749 1501
Size: 248 3822
NgoMIV G'CCGG_C
Cuts at: 2249 2249
Size: 4070
NspI r_CATG'y
Cuts at: 1123 1623 2530 1123
Size: 500 907 2663
Fragments arranged by size:
2663 907 500
PasI CC'CwG_GG
Cuts at: 564 564
Size: 4070
PciI A'CATG_T
Cuts at: 2526 2526
Size: 4070
PflMI CCAn_nnn'nTGG
Cuts at: 257 257
Size: 4070
PfoI T'CCnGG_A
Cuts at: 323 323
Size: 4070
PmeI GTTT'AAAC
Cuts at: 1069 1069
Size: 4070
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 142 174 792 824 3209 3241 142
Size: 32 618 32 2385 32 971
Fragments arranged by size:
2385 971 618 32 32 32
PspOMI G'GGCC_C
Cuts at: 380 413 560 380
Size: 33 147 3890
Fragments arranged by size:
3890 147 33
PstI C_TGCA'G
Cuts at: 593 875 889 1117 1802 593
Size: 282 14 228 685 2861
Fragments arranged by size:
2861 685 282 228 14
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4070
PvuII CAG'CTG
Cuts at: 1495 1855 1495
Size: 360 3710
RsrII CG'GwC_CG
Cuts at: 2265 2265
Size: 4070
SacI G_AGCT'C
Cuts at: 1084 1084
Size: 4070
SalI G'TCGA_C
Cuts at: 1107 1107
Size: 4070
SapI GCTCTTCn'nnn_
Cuts at: 2093 2303 2093
Size: 210 3860
SbfI CC_TGCA'GG
Cuts at: 1117 1117
Size: 4070
ScaI AGT'ACT
Cuts at: 3587 3587
Size: 4070
SfcI C'TryA_G
Cuts at: 33 589 871 885 1113 1798 2791 2982 33
Size: 556 282 14 228 685 993 191 1121
Fragments arranged by size:
1121 993 685 556 282 228 191 14
SfoI GGC'GCC
Cuts at: 1502 1750 1502
Size: 248 3822
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4070
SmaI CCC'GGG
Cuts at: 1092 1092
Size: 4070
SmlI C'TyrA_G
Cuts at: 1225 2140 2632 2894 1225
Size: 915 492 262 2401
Fragments arranged by size:
2401 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4070
SphI G_CATG'C
Cuts at: 1123 1623 1123
Size: 500 3570
StyI C'CwwG_G
Cuts at: 1204 1558 1204
Size: 354 3716
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 249 544 899 1653 1786 2245 2454 249
Size: 295 355 754 133 459 209 1865
Fragments arranged by size:
1865 754 459 355 295 209 133
TatI w'GTAC_w
Cuts at: 3585 3585
Size: 4070
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4038
Tth111I GACn'n_nGTC
Cuts at: 1867 1867
Size: 4070
XbaI T'CTAG_A
Cuts at: 1101 1101
Size: 4070
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 258 1565 258
Size: 1307 2763
XmaI C'CCGG_G
Cuts at: 1090 1090
Size: 4070
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI BmtI BpmI BsaXI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I ClaI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI