Restriction map for
pF1KE0863
(Circular) (Six-base) MAPSORT of: pF1KE0863.seq Check: 9925 from: 1 to: 4052
pF1KE0863 4052 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 838 838
Size: 4052
AccI GT'mk_AC
Cuts at: 209 1090 209
Size: 881 3171
Acc65I G'GTAC_C
Cuts at: 547 1068 547
Size: 521 3531
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 1357 2924 1357
Size: 1567 2485
ApaI G_GGCC'C
Cuts at: 184 1011 184
Size: 827 3225
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 139 1056 3405 3661 139
Size: 917 2349 256 530
Fragments arranged by size:
2349 917 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 547 778 1068 1482 1730 1765 547
Size: 231 290 414 248 35 2834
Fragments arranged by size:
2834 414 290 248 231 35
BanII G_rGCy'C
Cuts at: 184 320 367 523 1011 1066 2096 184
Size: 136 47 156 488 55 1030 2140
Fragments arranged by size:
2140 1030 488 156 136 55 47
BbeI G_GCGC'C
Cuts at: 1486 1734 1486
Size: 248 3804
BbsI GAAGACnn'nnnn_
Cuts at: 411 717 813 411
Size: 306 96 3650
Fragments arranged by size:
3650 306 96
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 239 1572 239
Size: 1333 2719
BglI GCCn_nnn'nGGC
Cuts at: 535 535
Size: 4052
BglII A'GATC_T
Cuts at: 698 1567 3604 698
Size: 869 2037 1146
Fragments arranged by size:
2037 1146 869
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 184 344 1011 1677 1770 2826 184
Size: 160 667 666 93 1056 1410
Fragments arranged by size:
1410 1056 667 666 160 93
BmgBI CAC'GTC
Cuts at: 999 999
Size: 4052
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 537 1228 2107 2599 2897 151
Size: 386 691 879 492 298 1306
Fragments arranged by size:
1306 879 691 492 386 298
BsaI GGTCTCn'nnnn_
Cuts at: 858 3756 858
Size: 2898 1154
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 1483 1731 2433 3929 1483
Size: 248 702 1496 1606
Fragments arranged by size:
1606 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 825 897 1017 238
Size: 587 72 120 3273
Fragments arranged by size:
3273 587 120 72
BseYI C'CCAG_C
Cuts at: 112 225 679 1477 2235 2812 3308 112
Size: 113 454 798 758 577 496 856
Fragments arranged by size:
856 798 758 577 496 454 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 422 256
Size: 166 3886
BsiEI CG_ry'CG
Cuts at: 104 895 1594 1640 2848 104
Size: 791 699 46 1208 1308
Fragments arranged by size:
1308 1208 791 699 46
BsiHKAI G_wGCw'C
Cuts at: 320 1066 1844 2034 2826 320
Size: 746 778 190 792 1546
Fragments arranged by size:
1546 792 778 746 190
Bsp1286I G_dGCh'C
Cuts at: 184 320 344 367 523 1011 1066 1677 1770
Size: 136 24 23 156 488 55 611 93
Cuts at: 1770 1844 2034 2096 2826 184
Size: 74 190 62 730 1410
Fragments arranged by size:
1410 730 611 488 190 156 136 93
74 62 55 24 23
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 838 1102 1316 1618 1999 838
Size: 264 214 302 381 2891
Fragments arranged by size:
2891 381 302 264 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 797 1964 797
Size: 1167 2885
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BssSI C'ACGA_G
Cuts at: 847 2323 2681 847
Size: 1476 358 2218
Fragments arranged by size:
2218 1476 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1429 1429
Size: 4052
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstEII G'GTnAC_C
Cuts at: 216 216
Size: 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 351 351
Size: 4052
BstYI r'GATC_y
Cuts at: 1 698 1077 1544 1567 1902 2148 3160 3246
Size: 697 379 467 23 335 246 1012 86
Cuts at: 3246 3258 3604 1
Size: 12 346 449
Fragments arranged by size:
1012 697 467 449 379 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 461 523 539 107
Size: 354 62 16 3620
Fragments arranged by size:
3620 354 62 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 130 1295 130
Size: 1165 2887
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DraIII CAC_nnn'GTG
Cuts at: 1023 1023
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 353 458 536 575 892 1637 1811 2202 2229
Size: 105 78 39 317 745 174 391 27
Cuts at: 2229 3574 353
Size: 1345 831
Fragments arranged by size:
1345 831 745 391 317 174 105 78
39 27
EagI C'GGCC_G
Cuts at: 892 1637 892
Size: 745 3307
EarI CTCTTCn'nnn_
Cuts at: 325 865 943 1021 2075 2285 325
Size: 540 78 78 1054 210 2092
Fragments arranged by size:
2092 1054 540 210 78 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 318 1064 318
Size: 746 3306
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1877 2309 3056 3324 1877
Size: 432 747 268 2605
Fragments arranged by size:
2605 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 253 253
Size: 4052
EcoO109I rG'GnC_Cy
Cuts at: 171 180 181 347 1007 1008 1191 171
Size: 9 1 166 660 1 183 3032
Fragments arranged by size:
3032 660 183 166 9 1 1
EcoRI G'AATT_C
Cuts at: 139 1056 139
Size: 917 3135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 1038 1486 1734 2756 3735 1038
Size: 448 248 1022 979 1355
Fragments arranged by size:
1355 1022 979 448 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1894 1924 1926 1956 1894
Size: 30 2 30 3990
Fragments arranged by size:
3990 30 30 2
HincII GTy'rAC
Cuts at: 1091 1091
Size: 4052
KasI G'GCGC_C
Cuts at: 1482 1730 1482
Size: 248 3804
KpnI G_GTAC'C
Cuts at: 551 1072 551
Size: 521 3531
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2723 2907 2723
Size: 184 3868
MscI TGG'CCA
Cuts at: 355 538 1813 355
Size: 183 1275 2594
Fragments arranged by size:
2594 1275 183
MslI CAynn'nnrTG
Cuts at: 460 538 958 2041 2168 460
Size: 78 420 1083 127 2344
Fragments arranged by size:
2344 1083 420 127 78
MspA1I CmG'CkG
Cuts at: 1163 1477 1837 2850 3095 1163
Size: 314 360 1013 245 2120
Fragments arranged by size:
2120 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 1483 1731 1483
Size: 248 3804
NcoI C'CATG_G
Cuts at: 107 461 539 107
Size: 354 78 3620
Fragments arranged by size:
3620 354 78
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 1105 1605 2512 1105
Size: 500 907 2645
Fragments arranged by size:
2645 907 500
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PpuMI rG'GwC_Cy
Cuts at: 347 347
Size: 4052
PspOMI G'GGCC_C
Cuts at: 180 1007 180
Size: 827 3225
PstI C_TGCA'G
Cuts at: 179 1099 1784 179
Size: 920 685 2447
Fragments arranged by size:
2447 920 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 1477 1837 1477
Size: 360 3692
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 320 1066 320
Size: 746 3306
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 325 2075 2285 325
Size: 1750 210 2092
Fragments arranged by size:
2092 1750 210
SbfI CC_TGCA'GG
Cuts at: 179 1099 179
Size: 920 3132
ScaI AGT'ACT
Cuts at: 662 3569 662
Size: 2907 1145
SexAI A'CCwGG_T
Cuts at: 212 212
Size: 4052
SfcI C'TryA_G
Cuts at: 33 175 1095 1780 2773 2964 33
Size: 142 920 685 993 191 1121
Fragments arranged by size:
1121 993 920 685 191 142
SfiI GGCCn_nnn'nGGCC
Cuts at: 535 535
Size: 4052
SfoI GGC'GCC
Cuts at: 1484 1732 1484
Size: 248 3804
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 166 516 1207 2122 2614 2876 166
Size: 350 691 915 492 262 1342
Fragments arranged by size:
1342 915 691 492 350 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StyI C'CwwG_G
Cuts at: 107 461 539 1186 1540 107
Size: 354 78 647 354 2619
Fragments arranged by size:
2619 647 354 354 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 183 488 598 766 817 972 1635 1768 2227
Size: 305 110 168 51 155 663 133 459
Cuts at: 2227 2436 183
Size: 209 1799
Fragments arranged by size:
1799 663 459 305 209 168 155 133
110 51
TatI w'GTAC_w
Cuts at: 284 660 3567 284
Size: 376 2907 769
Fragments arranged by size:
2907 769 376
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3569 3601 3569
Size: 32 4020
Tth111I GACn'n_nGTC
Cuts at: 925 1849 925
Size: 924 3128
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 789 1547 789
Size: 758 3294
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BpmI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI