Restriction map for
pF1KE0862
(Circular) (Six-base) MAPSORT of: pF1KE0862.seq Check: 5214 from: 1 to: 4052
pF1KE0862 4052 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1090 1090
Size: 4052
Acc65I G'GTAC_C
Cuts at: 1068 1068
Size: 4052
AclI AA'CG_TT
Cuts at: 3892 3892
Size: 4052
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 672 1877 2309 3056 3324 672
Size: 1205 432 747 268 1400
Fragments arranged by size:
1400 1205 747 432 268
AflII C'TTAA_G
Cuts at: 885 885
Size: 4052
AflIII A'CryG_T
Cuts at: 1404 2508 3485 1404
Size: 1104 977 1971
Fragments arranged by size:
1971 1104 977
AgeI A'CCGG_T
Cuts at: 2440 2440
Size: 4052
AhdI GACnn_n'nnGTC
Cuts at: 363 363
Size: 4052
AleI CACnn'nnGTG
Cuts at: 253 253
Size: 4052
AlwNI CAG_nnn'CTG
Cuts at: 183 1357 2924 183
Size: 1174 1567 1311
Fragments arranged by size:
1567 1311 1174
ApaLI G'TGCA_C
Cuts at: 2822 2822
Size: 4052
ApoI r'AATT_y
Cuts at: 994 1056 3405 3661 994
Size: 62 2349 256 1385
Fragments arranged by size:
2349 1385 256 62
AseI AT'TA_AT
Cuts at: 20 20
Size: 4052
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
AvaI C'yCGr_G
Cuts at: 1072 1072
Size: 4052
BamHI G'GATC_C
Cuts at: 1077 1077
Size: 4052
BanI G'GyrC_C
Cuts at: 226 1068 1482 1730 1765 226
Size: 842 414 248 35 2513
Fragments arranged by size:
2513 842 414 248 35
BanII G_rGCy'C
Cuts at: 270 1066 2096 270
Size: 796 1030 2226
Fragments arranged by size:
2226 1030 796
BbeI G_GCGC'C
Cuts at: 1486 1734 1486
Size: 248 3804
BbsI GAAGACnn'nnnn_
Cuts at: 382 382
Size: 4052
BciVI GTATCCnnnnn_n'
Cuts at: 1950 2711 4052 1950
Size: 761 1341 1950
Fragments arranged by size:
1950 1341 761
BclI T'GATC_A
Cuts at: 1572 1572
Size: 4052
BglII A'GATC_T
Cuts at: 1567 3604 1567
Size: 2037 2015
BlpI GC'TnA_GC
Cuts at: 1164 1164
Size: 4052
Bme1580I G_kGCm'C
Cuts at: 229 1677 1770 2826 229
Size: 1448 93 1056 1455
Fragments arranged by size:
1455 1448 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1264 1345 1680 3824 1264
Size: 81 335 2144 1492
Fragments arranged by size:
2144 1492 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2419 2419
Size: 4052
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1228 2107 2599 2897 1228
Size: 879 492 298 2383
Fragments arranged by size:
2383 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3756 3756
Size: 4052
BsaAI yAC'GTr
Cuts at: 1405 1405
Size: 4052
BsaBI GATnn'nnATC
Cuts at: 6 1566 3603 6
Size: 1560 2037 455
Fragments arranged by size:
2037 1560 455
BsaHI Gr'CG_yC
Cuts at: 1483 1731 2433 3929 1483
Size: 248 702 1496 1606
Fragments arranged by size:
1606 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1241 1762 2440 2714 2861 3272 1241
Size: 521 678 274 147 411 2021
Fragments arranged by size:
2021 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 196 423 606 196
Size: 227 183 3642
Fragments arranged by size:
3642 227 183
BseYI C'CCAG_C
Cuts at: 1477 2235 2812 3308 1477
Size: 758 577 496 2221
Fragments arranged by size:
2221 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1594 1640 2848 104
Size: 1490 46 1208 1308
Fragments arranged by size:
1490 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 512 1066 1844 2034 2826 512
Size: 554 778 190 792 1738
Fragments arranged by size:
1738 792 778 554 190
Bsp1286I G_dGCh'C
Cuts at: 229 270 512 1066 1677 1770 1844 2034 2096
Size: 41 242 554 611 93 74 190 62
Cuts at: 2096 2826 229
Size: 730 1455
Fragments arranged by size:
1455 730 611 554 242 190 93 74
62 41
BspEI T'CCGG_A
Cuts at: 3272 3272
Size: 4052
BspHI T'CATG_A
Cuts at: 3228 3228
Size: 4052
BspMI ACCTGCnnnn'nnnn_
Cuts at: 368 1102 1316 1618 1999 368
Size: 734 214 302 381 2421
Fragments arranged by size:
2421 734 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2344 2398 3882 4047 50
Size: 2294 54 1484 165 55
Fragments arranged by size:
2294 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1964 1964
Size: 4052
BsrFI r'CCGG_y
Cuts at: 2050 2231 2440 2050
Size: 181 209 3662
Fragments arranged by size:
3662 209 181
BssSI C'ACGA_G
Cuts at: 2323 2681 2323
Size: 358 3694
BstAPI GCAn_nnn'nTGC
Cuts at: 183 1429 183
Size: 1246 2806
BstBI TT'CG_AA
Cuts at: 2413 2413
Size: 4052
BstEII G'GTnAC_C
Cuts at: 579 579
Size: 4052
BstXI CCAn_nnnn'nTGG
Cuts at: 258 351 258
Size: 93 3959
BstYI r'GATC_y
Cuts at: 1 1077 1544 1567 1902 2148 3160 3246 3258
Size: 1076 467 23 335 246 1012 86 12
Cuts at: 3258 3604 1
Size: 346 449
Fragments arranged by size:
1076 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 458 107
Size: 351 3701
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2002 2183 2346 2002
Size: 181 163 3708
Fragments arranged by size:
3708 181 163
BtsI GCAGTG_nn'
Cuts at: 493 1295 493
Size: 802 3250
DraI TTT'AAA
Cuts at: 1051 1051
Size: 4052
DrdI GACnn_nn'nnGTC
Cuts at: 1758 2616 1758
Size: 858 3194
EaeI y'GGCC_r
Cuts at: 167 479 1023 1637 1811 2202 2229 3574 167
Size: 312 544 614 174 391 27 1345 645
Fragments arranged by size:
1345 645 614 544 391 312 174 27
EagI C'GGCC_G
Cuts at: 1637 1637
Size: 4052
EarI CTCTTCn'nnn_
Cuts at: 427 2075 2285 427
Size: 1648 210 2194
Fragments arranged by size:
2194 1648 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2260 2570 2716 3862 2260
Size: 310 146 1146 2450
Fragments arranged by size:
2450 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1064 1064
Size: 4052
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 672 1877 2309 3056 3324 672
Size: 1205 432 747 268 1400
Fragments arranged by size:
1400 1205 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1191 1191
Size: 4052
EcoRI G'AATT_C
Cuts at: 994 1056 994
Size: 62 3990
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2071 2071
Size: 4052
FspI TGC'GCA
Cuts at: 1833 1833
Size: 4052
HaeII r_GCGC'y
Cuts at: 1486 1734 2756 3735 1486
Size: 248 1022 979 1803
Fragments arranged by size:
1803 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 128 160 349 381 963 995 1894 1924 1926
Size: 32 189 32 582 32 899 30 2
Cuts at: 1926 1956 128
Size: 30 2224
Fragments arranged by size:
2224 899 582 189 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 357 1091 357
Size: 734 3318
KasI G'GCGC_C
Cuts at: 1482 1730 1482
Size: 248 3804
KpnI G_GTAC'C
Cuts at: 1072 1072
Size: 4052
MluI A'CGCG_T
Cuts at: 3485 3485
Size: 4052
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 644 783 2723 2907 644
Size: 139 1940 184 1789
Fragments arranged by size:
1940 1789 184 139
MscI TGG'CCA
Cuts at: 169 481 1813 169
Size: 312 1332 2408
Fragments arranged by size:
2408 1332 312
MslI CAynn'nnrTG
Cuts at: 253 406 706 2041 2168 253
Size: 153 300 1335 127 2137
Fragments arranged by size:
2137 1335 300 153 127
MspA1I CmG'CkG
Cuts at: 183 696 1163 1477 1837 2850 3095 183
Size: 513 467 314 360 1013 245 1140
Fragments arranged by size:
1140 1013 513 467 360 314 245
NaeI GCC'GGC
Cuts at: 2233 2233
Size: 4052
NarI GG'CG_CC
Cuts at: 1483 1731 1483
Size: 248 3804
NcoI C'CATG_G
Cuts at: 107 458 107
Size: 351 3701
NgoMIV G'CCGG_C
Cuts at: 2231 2231
Size: 4052
NspI r_CATG'y
Cuts at: 1105 1605 2512 1105
Size: 500 907 2645
Fragments arranged by size:
2645 907 500
PciI A'CATG_T
Cuts at: 2508 2508
Size: 4052
PmeI GTTT'AAAC
Cuts at: 1051 1051
Size: 4052
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 4020
PstI C_TGCA'G
Cuts at: 179 1099 1784 179
Size: 920 685 2447
Fragments arranged by size:
2447 920 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4052
PvuII CAG'CTG
Cuts at: 183 696 1477 1837 183
Size: 513 781 360 2398
Fragments arranged by size:
2398 781 513 360
RsrII CG'GwC_CG
Cuts at: 2247 2247
Size: 4052
SacI G_AGCT'C
Cuts at: 1066 1066
Size: 4052
SalI G'TCGA_C
Cuts at: 1089 1089
Size: 4052
SapI GCTCTTCn'nnn_
Cuts at: 2075 2285 2075
Size: 210 3842
SbfI CC_TGCA'GG
Cuts at: 1099 1099
Size: 4052
ScaI AGT'ACT
Cuts at: 3569 3569
Size: 4052
SfcI C'TryA_G
Cuts at: 33 175 861 1095 1780 2773 2964 33
Size: 142 686 234 685 993 191 1121
Fragments arranged by size:
1121 993 686 685 234 191 142
SfoI GGC'GCC
Cuts at: 1484 1732 1484
Size: 248 3804
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4052
SmaI CCC'GGG
Cuts at: 1074 1074
Size: 4052
SmlI C'TyrA_G
Cuts at: 885 1207 2122 2614 2876 885
Size: 322 915 492 262 2061
Fragments arranged by size:
2061 915 492 322 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4052
SphI G_CATG'C
Cuts at: 1105 1605 1105
Size: 500 3552
StyI C'CwwG_G
Cuts at: 107 458 725 1186 1540 107
Size: 351 267 461 354 2619
Fragments arranged by size:
2619 461 354 351 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1635 1768 2227 2436 1635
Size: 133 459 209 3251
Fragments arranged by size:
3251 459 209 133
TatI w'GTAC_w
Cuts at: 284 3567 284
Size: 3283 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 509 541 3569 3601 509
Size: 32 3028 32 960
Fragments arranged by size:
3028 960 32 32
Tth111I GACn'n_nGTC
Cuts at: 1849 1849
Size: 4052
XbaI T'CTAG_A
Cuts at: 1083 1083
Size: 4052
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 255 387 1547 255
Size: 132 1160 2760
Fragments arranged by size:
2760 1160 132
XmaI C'CCGG_G
Cuts at: 1072 1072
Size: 4052
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI BpmI
Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI