Restriction map for
pF1KE0861
(Circular) (Six-base) MAPSORT of: pF1KE0861.seq Check: 4047 from: 1 to: 3941
pF1KE0861 3941 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 979 979
Size: 3941
Acc65I G'GTAC_C
Cuts at: 957 957
Size: 3941
AclI AA'CG_TT
Cuts at: 409 3781 409
Size: 3372 569
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1766 2198 2945 3213 1766
Size: 432 747 268 2494
Fragments arranged by size:
2494 747 432 268
AflIII A'CryG_T
Cuts at: 1293 2397 3374 1293
Size: 1104 977 1860
Fragments arranged by size:
1860 1104 977
AgeI A'CCGG_T
Cuts at: 2329 2329
Size: 3941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 902 934 902
Size: 32 3909
AlwNI CAG_nnn'CTG
Cuts at: 1246 2813 1246
Size: 1567 2374
ApaLI G'TGCA_C
Cuts at: 2711 2711
Size: 3941
ApoI r'AATT_y
Cuts at: 945 3294 3550 945
Size: 2349 256 1336
Fragments arranged by size:
2349 1336 256
AseI AT'TA_AT
Cuts at: 20 674 20
Size: 654 3287
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
AvaI C'yCGr_G
Cuts at: 961 961
Size: 3941
BamHI G'GATC_C
Cuts at: 966 966
Size: 3941
BanI G'GyrC_C
Cuts at: 957 1371 1619 1654 957
Size: 414 248 35 3244
Fragments arranged by size:
3244 414 248 35
BanII G_rGCy'C
Cuts at: 955 1985 955
Size: 1030 2911
BbeI G_GCGC'C
Cuts at: 1375 1623 1375
Size: 248 3693
BbsI GAAGACnn'nnnn_
Cuts at: 738 817 738
Size: 79 3862
BbvCI CC'TCA_GC
Cuts at: 792 797 792
Size: 5 3936
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3907
BciVI GTATCCnnnnn_n'
Cuts at: 1839 2600 3941 1839
Size: 761 1341 1839
Fragments arranged by size:
1839 1341 761
BclI T'GATC_A
Cuts at: 1461 1461
Size: 3941
BglII A'GATC_T
Cuts at: 1456 3493 1456
Size: 2037 1904
BlpI GC'TnA_GC
Cuts at: 1053 1053
Size: 3941
Bme1580I G_kGCm'C
Cuts at: 1566 1659 2715 1566
Size: 93 1056 2792
Fragments arranged by size:
2792 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1153 1234 1569 3713 1153
Size: 81 335 2144 1381
Fragments arranged by size:
2144 1381 335 81
BmtI G_CTAG'C
Cuts at: 311 311
Size: 3941
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 3941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 339 144
Size: 195 3746
Bpu10I CC'TnA_GC
Cuts at: 295 792 797 295
Size: 497 5 3439
Fragments arranged by size:
3439 497 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 516 766 1117 1996 2488 2786 516
Size: 250 351 879 492 298 1671
Fragments arranged by size:
1671 879 492 351 298 250
BsaI GGTCTCn'nnnn_
Cuts at: 3645 3645
Size: 3941
BsaAI yAC'GTr
Cuts at: 1294 1294
Size: 3941
BsaBI GATnn'nnATC
Cuts at: 6 1455 3492 6
Size: 1449 2037 455
Fragments arranged by size:
2037 1449 455
BsaHI Gr'CG_yC
Cuts at: 1372 1620 2322 3818 1372
Size: 248 702 1496 1495
Fragments arranged by size:
1496 1495 702 248
BsaWI w'CCGG_w
Cuts at: 1130 1651 2329 2603 2750 3161 1130
Size: 521 678 274 147 411 1910
Fragments arranged by size:
1910 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 902 932 902
Size: 30 3911
BseYI C'CCAG_C
Cuts at: 374 1366 2124 2701 3197 374
Size: 992 758 577 496 1118
Fragments arranged by size:
1118 992 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1483 1529 2737 104
Size: 1379 46 1208 1308
Fragments arranged by size:
1379 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 955 1733 1923 2715 955
Size: 778 190 792 2181
Fragments arranged by size:
2181 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 955 1566 1659 1733 1923 1985 2715 955
Size: 611 93 74 190 62 730 2181
Fragments arranged by size:
2181 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3161 3161
Size: 3941
BspHI T'CATG_A
Cuts at: 242 656 3117 242
Size: 414 2461 1066
Fragments arranged by size:
2461 1066 414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 382 991 1205 1507 1888 382
Size: 609 214 302 381 2435
Fragments arranged by size:
2435 609 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2233 2287 3771 3936 50
Size: 2183 54 1484 165 55
Fragments arranged by size:
2183 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 237 872 1853 237
Size: 635 981 2325
Fragments arranged by size:
2325 981 635
BsrFI r'CCGG_y
Cuts at: 1939 2120 2329 1939
Size: 181 209 3551
Fragments arranged by size:
3551 209 181
BsrGI T'GTAC_A
Cuts at: 209 323 209
Size: 114 3827
BssSI C'ACGA_G
Cuts at: 2212 2570 2212
Size: 358 3583
BstAPI GCAn_nnn'nTGC
Cuts at: 1318 1318
Size: 3941
BstBI TT'CG_AA
Cuts at: 2302 2302
Size: 3941
BstXI CCAn_nnnn'nTGG
Cuts at: 741 741
Size: 3941
BstYI r'GATC_y
Cuts at: 1 966 1433 1456 1791 2037 3049 3135 3147
Size: 965 467 23 335 246 1012 86 12
Cuts at: 3147 3493 1
Size: 346 449
Fragments arranged by size:
1012 965 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 569 734 569
Size: 165 3776
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1891 2072 2235 1891
Size: 181 163 3597
Fragments arranged by size:
3597 181 163
BtsI GCAGTG_nn'
Cuts at: 1184 1184
Size: 3941
DraI TTT'AAA
Cuts at: 940 940
Size: 3941
DraIII CAC_nnn'GTG
Cuts at: 621 621
Size: 3941
DrdI GACnn_nn'nnGTC
Cuts at: 1647 2505 1647
Size: 858 3083
EaeI y'GGCC_r
Cuts at: 428 479 566 1526 1700 2091 2118 3463 428
Size: 51 87 960 174 391 27 1345 906
Fragments arranged by size:
1345 960 906 391 174 87 51 27
EagI C'GGCC_G
Cuts at: 1526 1526
Size: 3941
EarI CTCTTCn'nnn_
Cuts at: 1964 2174 1964
Size: 210 3731
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2149 2459 2605 3751 2149
Size: 310 146 1146 2339
Fragments arranged by size:
2339 1146 310 146
EcoICRI GAG'CTC
Cuts at: 953 953
Size: 3941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 339 1766 2198 2945 3213 144
Size: 195 1427 432 747 268 872
Fragments arranged by size:
1427 872 747 432 268 195
EcoNI CCTnn'n_nnAGG
Cuts at: 127 127
Size: 3941
EcoO109I rG'GnC_Cy
Cuts at: 1080 1080
Size: 3941
EcoRI G'AATT_C
Cuts at: 945 945
Size: 3941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 831 937 1960 831
Size: 106 1023 2812
Fragments arranged by size:
2812 1023 106
FspI TGC'GCA
Cuts at: 1722 1722
Size: 3941
HaeII r_GCGC'y
Cuts at: 1375 1623 2645 3624 1375
Size: 248 1022 979 1692
Fragments arranged by size:
1692 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 1783 1813 1815 1845 491
Size: 32 1260 30 2 30 2587
Fragments arranged by size:
2587 1260 32 30 30 2
HincII GTy'rAC
Cuts at: 980 980
Size: 3941
KasI G'GCGC_C
Cuts at: 1371 1619 1371
Size: 248 3693
KpnI G_GTAC'C
Cuts at: 961 961
Size: 3941
MfeI C'AATT_G
Cuts at: 438 438
Size: 3941
MluI A'CGCG_T
Cuts at: 3374 3374
Size: 3941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 784 2612 2796 784
Size: 1828 184 1929
Fragments arranged by size:
1929 1828 184
MscI TGG'CCA
Cuts at: 430 481 568 1702 430
Size: 51 87 1134 2669
Fragments arranged by size:
2669 1134 87 51
MslI CAynn'nnrTG
Cuts at: 241 280 457 1930 2057 241
Size: 39 177 1473 127 2125
Fragments arranged by size:
2125 1473 177 127 39
MspA1I CmG'CkG
Cuts at: 537 577 796 1052 1366 1726 2739 2984 537
Size: 40 219 256 314 360 1013 245 1494
Fragments arranged by size:
1494 1013 360 314 256 245 219 40
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 3941
NarI GG'CG_CC
Cuts at: 1372 1620 1372
Size: 248 3693
NcoI C'CATG_G
Cuts at: 569 734 569
Size: 165 3776
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 3941
NheI G'CTAG_C
Cuts at: 307 307
Size: 3941
NspI r_CATG'y
Cuts at: 303 994 1494 2401 303
Size: 691 500 907 1843
Fragments arranged by size:
1843 907 691 500
PciI A'CATG_T
Cuts at: 2397 2397
Size: 3941
PflMI CCAn_nnn'nTGG
Cuts at: 477 477
Size: 3941
PmeI GTTT'AAAC
Cuts at: 940 940
Size: 3941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 902 934 3080 3112 902
Size: 32 2146 32 1731
Fragments arranged by size:
2146 1731 32 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 316 348 316
Size: 32 3909
PstI C_TGCA'G
Cuts at: 988 1673 988
Size: 685 3256
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3941
PvuII CAG'CTG
Cuts at: 537 577 796 1366 1726 537
Size: 40 219 570 360 2752
Fragments arranged by size:
2752 570 360 219 40
RsrII CG'GwC_CG
Cuts at: 2136 2136
Size: 3941
SacI G_AGCT'C
Cuts at: 955 955
Size: 3941
SalI G'TCGA_C
Cuts at: 978 978
Size: 3941
SapI GCTCTTCn'nnn_
Cuts at: 1964 2174 1964
Size: 210 3731
SbfI CC_TGCA'GG
Cuts at: 988 988
Size: 3941
ScaI AGT'ACT
Cuts at: 3458 3458
Size: 3941
SfcI C'TryA_G
Cuts at: 33 905 984 1669 2662 2853 33
Size: 872 79 685 993 191 1121
Fragments arranged by size:
1121 993 872 685 191 79
SfoI GGC'GCC
Cuts at: 1373 1621 1373
Size: 248 3693
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
SmaI CCC'GGG
Cuts at: 963 963
Size: 3941
SmlI C'TyrA_G
Cuts at: 495 781 1096 2011 2503 2765 495
Size: 286 315 915 492 262 1671
Fragments arranged by size:
1671 915 492 315 286 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3941
SphI G_CATG'C
Cuts at: 303 994 1494 303
Size: 691 500 2750
Fragments arranged by size:
2750 691 500
StyI C'CwwG_G
Cuts at: 425 569 734 1075 1429 425
Size: 144 165 341 354 2937
Fragments arranged by size:
2937 354 341 165 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 418 1524 1657 2116 2325 418
Size: 1106 133 459 209 2034
Fragments arranged by size:
2034 1106 459 209 133
TatI w'GTAC_w
Cuts at: 209 284 323 3456 209
Size: 75 39 3133 694
Fragments arranged by size:
3133 694 75 39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 3909
Tth111I GACn'n_nGTC
Cuts at: 1738 1738
Size: 3941
XbaI T'CTAG_A
Cuts at: 972 972
Size: 3941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 541 1436 541
Size: 895 3046
XmaI C'CCGG_G
Cuts at: 961 961
Size: 3941
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NspI PciI
PflMI PmeI PpiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BseRI BsgI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HindIII HpaI NdeI NotI
NruI NsiI PacI PasI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI