Restriction map for
pF1KE0860
(Circular) (Six-base) MAPSORT of: pF1KE0860.seq Check: 6213 from: 1 to: 4040
pF1KE0860 4040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 209 626 1078 209
Size: 417 452 3171
Fragments arranged by size:
3171 452 417
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 2297 3044 3312 1865
Size: 432 747 268 2593
Fragments arranged by size:
2593 747 432 268
AflIII A'CryG_T
Cuts at: 1392 2496 3473 1392
Size: 1104 977 1959
Fragments arranged by size:
1959 1104 977
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AleI CACnn'nnGTG
Cuts at: 130 130
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaLI G'TGCA_C
Cuts at: 2810 2810
Size: 4040
ApoI r'AATT_y
Cuts at: 139 1044 3393 3649 139
Size: 905 2349 256 530
Fragments arranged by size:
2349 905 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
BamHI G'GATC_C
Cuts at: 1065 1065
Size: 4040
BanI G'GyrC_C
Cuts at: 1056 1470 1718 1753 1056
Size: 414 248 35 3343
Fragments arranged by size:
3343 414 248 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbsI GAAGACnn'nnnn_
Cuts at: 817 817
Size: 4040
BbvCI CC'TCA_GC
Cuts at: 792 797 792
Size: 5 4035
BciVI GTATCCnnnnn_n'
Cuts at: 1938 2699 4040 1938
Size: 761 1341 1938
Fragments arranged by size:
1938 1341 761
BclI T'GATC_A
Cuts at: 1560 1560
Size: 4040
BglII A'GATC_T
Cuts at: 1555 3592 1555
Size: 2037 2003
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 534 734 1665 1758 2814 534
Size: 200 931 93 1056 1760
Fragments arranged by size:
1760 1056 931 200 93
BmrI ACTGGGnnnn_n'
Cuts at: 646 1252 1333 1668 3812 646
Size: 606 81 335 2144 874
Fragments arranged by size:
2144 874 606 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
Bpu10I CC'TnA_GC
Cuts at: 295 792 797 295
Size: 497 5 3538
Fragments arranged by size:
3538 497 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 868 1216 2095 2587 2885 868
Size: 348 879 492 298 2023
Fragments arranged by size:
2023 879 492 348 298
BsaI GGTCTCn'nnnn_
Cuts at: 3744 3744
Size: 4040
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 748 1554 3591 6
Size: 742 806 2037 455
Fragments arranged by size:
2037 806 742 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 533 1465 2223 2800 3296 533
Size: 932 758 577 496 1277
Fragments arranged by size:
1277 932 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1054 1832 2022 2814 1054
Size: 778 190 792 2280
Fragments arranged by size:
2280 792 778 190
BsmI GAATG_Cn'
Cuts at: 353 353
Size: 4040
BsmBI CGTCTCn'nnnn_
Cuts at: 310 310
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 534 734 1054 1665 1758 1832 2022 2084 2814
Size: 200 320 611 93 74 190 62 730
Cuts at: 2814 534
Size: 1760
Fragments arranged by size:
1760 730 611 320 200 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 512 656 3216 512
Size: 144 2560 1336
Fragments arranged by size:
2560 1336 144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1090 1304 1606 1987 1090
Size: 214 302 381 3143
Fragments arranged by size:
3143 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 464 872 1952 464
Size: 408 1080 2552
Fragments arranged by size:
2552 1080 408
BsrFI r'CCGG_y
Cuts at: 2038 2219 2428 2038
Size: 181 209 3650
Fragments arranged by size:
3650 209 181
BsrGI T'GTAC_A
Cuts at: 323 323
Size: 4040
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 397 1417 397
Size: 1020 3020
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 1065 1532 1555 1890 2136 3148 3234 3246
Size: 1064 467 23 335 246 1012 86 12
Cuts at: 3246 3592 1
Size: 346 449
Fragments arranged by size:
1064 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1283
Size: 4040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 619 654 619
Size: 35 4005
DraI TTT'AAA
Cuts at: 1039 1039
Size: 4040
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 428 479 1625 1799 2190 2217 3562 428
Size: 51 1146 174 391 27 1345 906
Fragments arranged by size:
1345 1146 906 391 174 51 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 106 777 2063 2273 106
Size: 671 1286 210 1873
Fragments arranged by size:
1873 1286 671 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1865 2297 3044 3312 1865
Size: 432 747 268 2593
Fragments arranged by size:
2593 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 548 1179 548
Size: 631 3409
EcoRI G'AATT_C
Cuts at: 139 1044 139
Size: 905 3135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 831 2059 831
Size: 1228 2812
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 491 523 1882 1912 1914 1944 491
Size: 32 1359 30 2 30 2587
Fragments arranged by size:
2587 1359 32 30 30 2
HincII GTy'rAC
Cuts at: 1079 1079
Size: 4040
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 430 481 1801 430
Size: 51 1320 2669
Fragments arranged by size:
2669 1320 51
MslI CAynn'nnrTG
Cuts at: 130 241 280 2029 2156 130
Size: 111 39 1749 127 2014
Fragments arranged by size:
2014 1749 127 111 39
MspA1I CmG'CkG
Cuts at: 537 796 1151 1465 1825 2838 3083 537
Size: 259 355 314 360 1013 245 1494
Fragments arranged by size:
1494 1013 360 355 314 259 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 303 1093 1593 2500 303
Size: 790 500 907 1843
Fragments arranged by size:
1843 907 790 500
PciI A'CATG_T
Cuts at: 2496 2496
Size: 4040
PflMI CCAn_nnn'nTGG
Cuts at: 613 613
Size: 4040
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PpuMI rG'GwC_Cy
Cuts at: 548 548
Size: 4040
PstI C_TGCA'G
Cuts at: 490 1087 1772 490
Size: 597 685 2758
Fragments arranged by size:
2758 685 597
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 537 796 1465 1825 537
Size: 259 669 360 2752
Fragments arranged by size:
2752 669 360 259
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 3557 3557
Size: 4040
SfcI C'TryA_G
Cuts at: 33 486 1083 1768 2761 2952 33
Size: 453 597 685 993 191 1121
Fragments arranged by size:
1121 993 685 597 453 191
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 883 1195 2110 2602 2864 883
Size: 312 915 492 262 2059
Fragments arranged by size:
2059 915 492 312 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4040
SphI G_CATG'C
Cuts at: 303 1093 1593 303
Size: 790 500 2750
Fragments arranged by size:
2750 790 500
SspI AAT'ATT
Cuts at: 1029 1029
Size: 4040
StuI AGG'CCT
Cuts at: 740 740
Size: 4040
StyI C'CwwG_G
Cuts at: 386 1174 1528 386
Size: 788 354 2898
Fragments arranged by size:
2898 788 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 323 3555 323
Size: 3232 808
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 541 1535 541
Size: 994 3046
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
XmnI GAAnn'nnTTC
Cuts at: 1029 1029
Size: 4040
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BssHII BstEII BstXI
BstZ17I Bsu36I BtgI ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI ZraI