Restriction map for
pF1KE0859
(Circular) (Six-base) MAPSORT of: pF1KE0859.seq Check: 5467 from: 1 to: 4100
pF1KE0859 4100 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 668 838 668
Size: 170 3930
AccI GT'mk_AC
Cuts at: 1138 1138
Size: 4100
Acc65I G'GTAC_C
Cuts at: 1116 1116
Size: 4100
AclI AA'CG_TT
Cuts at: 3940 3940
Size: 4100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 685 824 910 1004 1925 2357 3104 3372
Size: 535 139 86 94 921 432 747 268
Cuts at: 3372 150
Size: 878
Fragments arranged by size:
921 878 747 535 432 268 139 94
86
AflII C'TTAA_G
Cuts at: 787 787
Size: 4100
AflIII A'CryG_T
Cuts at: 1452 2556 3533 1452
Size: 1104 977 2019
Fragments arranged by size:
2019 1104 977
AgeI A'CCGG_T
Cuts at: 2488 2488
Size: 4100
AleI CACnn'nnGTG
Cuts at: 130 130
Size: 4100
AlwNI CAG_nnn'CTG
Cuts at: 722 1405 2972 722
Size: 683 1567 1850
Fragments arranged by size:
1850 1567 683
ApaLI G'TGCA_C
Cuts at: 2870 2870
Size: 4100
ApoI r'AATT_y
Cuts at: 120 1104 3453 3709 120
Size: 984 2349 256 511
Fragments arranged by size:
2349 984 511 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4100
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
AvaI C'yCGr_G
Cuts at: 1120 1120
Size: 4100
BamHI G'GATC_C
Cuts at: 1125 1125
Size: 4100
BanI G'GyrC_C
Cuts at: 1116 1530 1778 1813 1116
Size: 414 248 35 3403
Fragments arranged by size:
3403 414 248 35
BanII G_rGCy'C
Cuts at: 1114 2144 1114
Size: 1030 3070
BbeI G_GCGC'C
Cuts at: 1534 1782 1534
Size: 248 3852
BbsI GAAGACnn'nnnn_
Cuts at: 180 192 180
Size: 12 4088
BbvCI CC'TCA_GC
Cuts at: 798 798
Size: 4100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4066
BciVI GTATCCnnnnn_n'
Cuts at: 544 1998 2759 4100 544
Size: 1454 761 1341 544
Fragments arranged by size:
1454 1341 761 544
BclI T'GATC_A
Cuts at: 1620 1620
Size: 4100
BglII A'GATC_T
Cuts at: 1615 3652 1615
Size: 2037 2063
BlpI GC'TnA_GC
Cuts at: 1212 1212
Size: 4100
Bme1580I G_kGCm'C
Cuts at: 1725 1818 2874 1725
Size: 93 1056 2951
Fragments arranged by size:
2951 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 565 1312 1393 1728 3872 565
Size: 747 81 335 2144 793
Fragments arranged by size:
2144 793 747 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2467 2467
Size: 4100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 144
Size: 4100
Bpu10I CC'TnA_GC
Cuts at: 798 798
Size: 4100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 2155 2647 2945 1276
Size: 879 492 298 2431
Fragments arranged by size:
2431 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 313 367 3804 313
Size: 54 3437 609
Fragments arranged by size:
3437 609 54
BsaAI yAC'GTr
Cuts at: 1453 1453
Size: 4100
BsaBI GATnn'nnATC
Cuts at: 6 1614 3651 6
Size: 1608 2037 455
Fragments arranged by size:
2037 1608 455
BsaHI Gr'CG_yC
Cuts at: 1531 1779 2481 3977 1531
Size: 248 702 1496 1654
Fragments arranged by size:
1654 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1289 1810 2488 2762 2909 3320 1289
Size: 521 678 274 147 411 2069
Fragments arranged by size:
2069 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1024 1054 1024
Size: 30 4070
BseYI C'CCAG_C
Cuts at: 1525 2283 2860 3356 1525
Size: 758 577 496 2269
Fragments arranged by size:
2269 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 660 995 660
Size: 335 3765
BsiEI CG_ry'CG
Cuts at: 104 1642 1688 2896 104
Size: 1538 46 1208 1308
Fragments arranged by size:
1538 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1114 1892 2082 2874 1114
Size: 778 190 792 2340
Fragments arranged by size:
2340 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1114 1725 1818 1892 2082 2144 2874 1114
Size: 611 93 74 190 62 730 2340
Fragments arranged by size:
2340 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3320 3320
Size: 4100
BspHI T'CATG_A
Cuts at: 245 3276 245
Size: 3031 1069
BspMI ACCTGCnnnn'nnnn_
Cuts at: 224 331 668 838 1150 1364 1666 2047 224
Size: 107 337 170 312 214 302 381 2277
Fragments arranged by size:
2277 381 337 312 302 214 170 107
BsrBI CCG'CTC
Cuts at: 50 2392 2446 3930 4095 50
Size: 2342 54 1484 165 55
Fragments arranged by size:
2342 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 240 383 2012 240
Size: 143 1629 2328
Fragments arranged by size:
2328 1629 143
BsrFI r'CCGG_y
Cuts at: 2098 2279 2488 2098
Size: 181 209 3710
Fragments arranged by size:
3710 209 181
BsrGI T'GTAC_A
Cuts at: 975 975
Size: 4100
BssSI C'ACGA_G
Cuts at: 2371 2729 2371
Size: 358 3742
BstAPI GCAn_nnn'nTGC
Cuts at: 1477 1477
Size: 4100
BstBI TT'CG_AA
Cuts at: 2461 2461
Size: 4100
BstYI r'GATC_y
Cuts at: 1 1125 1592 1615 1950 2196 3208 3294 3306
Size: 1124 467 23 335 246 1012 86 12
Cuts at: 3306 3652 1
Size: 346 449
Fragments arranged by size:
1124 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 499 499
Size: 4100
BtgI C'CryG_G
Cuts at: 1020 1020
Size: 4100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2050 2231 2394 2050
Size: 181 163 3756
Fragments arranged by size:
3756 181 163
BtsI GCAGTG_nn'
Cuts at: 1343 1343
Size: 4100
DraI TTT'AAA
Cuts at: 1099 1099
Size: 4100
DrdI GACnn_nn'nnGTC
Cuts at: 688 1806 2664 688
Size: 1118 858 2124
Fragments arranged by size:
2124 1118 858
EaeI y'GGCC_r
Cuts at: 335 482 575 1685 1859 2250 2277 3622 335
Size: 147 93 1110 174 391 27 1345 813
Fragments arranged by size:
1345 1110 813 391 174 147 93 27
EagI C'GGCC_G
Cuts at: 1685 1685
Size: 4100
EarI CTCTTCn'nnn_
Cuts at: 418 1045 2123 2333 418
Size: 627 1078 210 2185
Fragments arranged by size:
2185 1078 627 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2308 2618 2764 3910 2308
Size: 310 146 1146 2498
Fragments arranged by size:
2498 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1112 1112
Size: 4100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 144 150 685 824 910 1004 1925 2357 3104
Size: 6 535 139 86 94 921 432 747
Cuts at: 3104 3372 144
Size: 268 872
Fragments arranged by size:
921 872 747 535 432 268 139 94
86 6
EcoNI CCTnn'n_nnAGG
Cuts at: 916 916
Size: 4100
EcoO109I rG'GnC_Cy
Cuts at: 527 1239 527
Size: 712 3388
EcoRI G'AATT_C
Cuts at: 1104 1104
Size: 4100
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 837 2119 837
Size: 1282 2818
FspI TGC'GCA
Cuts at: 1881 1881
Size: 4100
HaeII r_GCGC'y
Cuts at: 1534 1782 2804 3783 1534
Size: 248 1022 979 1851
Fragments arranged by size:
1851 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 930 962 1942 1972 1974 2004 930
Size: 32 980 30 2 30 3026
Fragments arranged by size:
3026 980 32 30 30 2
HincII GTy'rAC
Cuts at: 1139 1139
Size: 4100
KasI G'GCGC_C
Cuts at: 1530 1778 1530
Size: 248 3852
KpnI G_GTAC'C
Cuts at: 1120 1120
Size: 4100
MluI A'CGCG_T
Cuts at: 3533 3533
Size: 4100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 561 2771 2955 561
Size: 2210 184 1706
Fragments arranged by size:
2210 1706 184
MscI TGG'CCA
Cuts at: 337 484 577 1861 337
Size: 147 93 1284 2576
Fragments arranged by size:
2576 1284 147 93
MslI CAynn'nnrTG
Cuts at: 130 283 460 934 2089 2216 130
Size: 153 177 474 1155 127 2014
Fragments arranged by size:
2014 1155 474 177 153 127
MspA1I CmG'CkG
Cuts at: 802 1211 1525 1885 2898 3143 802
Size: 409 314 360 1013 245 1759
Fragments arranged by size:
1759 1013 409 360 314 245
NaeI GCC'GGC
Cuts at: 2281 2281
Size: 4100
NarI GG'CG_CC
Cuts at: 1531 1779 1531
Size: 248 3852
NdeI CA'TA_TG
Cuts at: 350 350
Size: 4100
NgoMIV G'CCGG_C
Cuts at: 2279 2279
Size: 4100
NspI r_CATG'y
Cuts at: 306 1153 1653 2560 306
Size: 847 500 907 1846
Fragments arranged by size:
1846 907 847 500
PasI CC'CwG_GG
Cuts at: 912 912
Size: 4100
PciI A'CATG_T
Cuts at: 2556 2556
Size: 4100
PmeI GTTT'AAAC
Cuts at: 1099 1099
Size: 4100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3239 3271 3239
Size: 32 4068
PsiI TTA'TAA
Cuts at: 1084 1084
Size: 4100
PstI C_TGCA'G
Cuts at: 1147 1832 1147
Size: 685 3415
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4100
PvuII CAG'CTG
Cuts at: 802 1525 1885 802
Size: 723 360 3017
Fragments arranged by size:
3017 723 360
RsrII CG'GwC_CG
Cuts at: 2295 2295
Size: 4100
SacI G_AGCT'C
Cuts at: 1114 1114
Size: 4100
SalI G'TCGA_C
Cuts at: 1137 1137
Size: 4100
SapI GCTCTTCn'nnn_
Cuts at: 418 2123 2333 418
Size: 1705 210 2185
Fragments arranged by size:
2185 1705 210
SbfI CC_TGCA'GG
Cuts at: 1147 1147
Size: 4100
ScaI AGT'ACT
Cuts at: 3617 3617
Size: 4100
SfcI C'TryA_G
Cuts at: 33 1143 1828 2821 3012 33
Size: 1110 685 993 191 1121
Fragments arranged by size:
1121 1110 993 685 191
SfoI GGC'GCC
Cuts at: 1532 1780 1532
Size: 248 3852
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4100
SmaI CCC'GGG
Cuts at: 1122 1122
Size: 4100
SmlI C'TyrA_G
Cuts at: 787 1255 2170 2662 2924 787
Size: 468 915 492 262 1963
Fragments arranged by size:
1963 915 492 468 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4100
SphI G_CATG'C
Cuts at: 306 1153 1653 306
Size: 847 500 2753
Fragments arranged by size:
2753 847 500
StuI AGG'CCT
Cuts at: 820 820
Size: 4100
StyI C'CwwG_G
Cuts at: 567 1234 1588 567
Size: 667 354 3079
Fragments arranged by size:
3079 667 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 406 1683 1816 2275 2484 406
Size: 1277 133 459 209 2022
Fragments arranged by size:
2022 1277 459 209 133
TatI w'GTAC_w
Cuts at: 287 975 3615 287
Size: 688 2640 772
Fragments arranged by size:
2640 772 688
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3617 3649 3617
Size: 32 4068
Tth111I GACn'n_nGTC
Cuts at: 1897 1897
Size: 4100
XbaI T'CTAG_A
Cuts at: 1131 1131
Size: 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 258 355 544 1595 258
Size: 97 189 1051 2763
Fragments arranged by size:
2763 1051 189 97
XmaI C'CCGG_G
Cuts at: 1120 1120
Size: 4100
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PasI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BfrBI BglI BmgBI BmtI BseRI BsiWI BsmI
BsmBI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI