Restriction map for
pF1KE0858
(Circular) (Six-base) MAPSORT of: pF1KE0858.seq Check: 9376 from: 1 to: 4058
pF1KE0858 4058 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 541 541
Size: 4058
AccI GT'mk_AC
Cuts at: 1096 1096
Size: 4058
Acc65I G'GTAC_C
Cuts at: 1074 1074
Size: 4058
AclI AA'CG_TT
Cuts at: 3898 3898
Size: 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 405 824 910 963 1021 1883 2315 3062 3330
Size: 419 86 53 58 862 432 747 268
Cuts at: 3330 405
Size: 1133
Fragments arranged by size:
1133 862 747 432 419 268 86 58
53
AfeI AGC'GCT
Cuts at: 376 376
Size: 4058
AflIII A'CryG_T
Cuts at: 1410 2514 3491 1410
Size: 1104 977 1977
Fragments arranged by size:
1977 1104 977
AgeI A'CCGG_T
Cuts at: 2446 2446
Size: 4058
AhdI GACnn_n'nnGTC
Cuts at: 363 363
Size: 4058
AleI CACnn'nnGTG
Cuts at: 130 850 130
Size: 720 3338
AlwNI CAG_nnn'CTG
Cuts at: 1363 2930 1363
Size: 1567 2491
ApaLI G'TGCA_C
Cuts at: 859 2828 859
Size: 1969 2089
ApoI r'AATT_y
Cuts at: 139 1062 3411 3667 139
Size: 923 2349 256 530
Fragments arranged by size:
2349 923 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4058
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
AvaI C'yCGr_G
Cuts at: 1078 1078
Size: 4058
BamHI G'GATC_C
Cuts at: 1083 1083
Size: 4058
BanI G'GyrC_C
Cuts at: 1074 1488 1736 1771 1074
Size: 414 248 35 3361
Fragments arranged by size:
3361 414 248 35
BanII G_rGCy'C
Cuts at: 1072 2102 1072
Size: 1030 3028
BbeI G_GCGC'C
Cuts at: 1492 1740 1492
Size: 248 3810
BbsI GAAGACnn'nnnn_
Cuts at: 1024 1024
Size: 4058
BbvCI CC'TCA_GC
Cuts at: 964 964
Size: 4058
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4024
BciVI GTATCCnnnnn_n'
Cuts at: 1956 2717 4058 1956
Size: 761 1341 1956
Fragments arranged by size:
1956 1341 761
BclI T'GATC_A
Cuts at: 1578 1578
Size: 4058
BglI GCCn_nnn'nGGC
Cuts at: 391 524 542 391
Size: 133 18 3907
Fragments arranged by size:
3907 133 18
BglII A'GATC_T
Cuts at: 1573 3610 1573
Size: 2037 2021
BlpI GC'TnA_GC
Cuts at: 1170 1170
Size: 4058
Bme1580I G_kGCm'C
Cuts at: 303 863 1683 1776 2832 303
Size: 560 820 93 1056 1529
Fragments arranged by size:
1529 1056 820 560 93
BmrI ACTGGGnnnn_n'
Cuts at: 900 1270 1351 1686 3830 900
Size: 370 81 335 2144 1128
Fragments arranged by size:
2144 1128 370 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 4058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 276 160
Size: 116 3942
Bpu10I CC'TnA_GC
Cuts at: 964 964
Size: 4058
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 2113 2605 2903 1234
Size: 879 492 298 2389
Fragments arranged by size:
2389 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3762 3762
Size: 4058
BsaAI yAC'GTr
Cuts at: 477 648 1411 477
Size: 171 763 3124
Fragments arranged by size:
3124 763 171
BsaBI GATnn'nnATC
Cuts at: 6 934 1572 3609 6
Size: 928 638 2037 455
Fragments arranged by size:
2037 928 638 455
BsaHI Gr'CG_yC
Cuts at: 1489 1737 2439 3935 1489
Size: 248 702 1496 1612
Fragments arranged by size:
1612 1496 702 248
BsaWI w'CCGG_w
Cuts at: 912 1247 1768 2446 2720 2867 3278 912
Size: 335 521 678 274 147 411 1692
Fragments arranged by size:
1692 678 521 411 335 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 112 142 668 698 112
Size: 30 526 30 3472
Fragments arranged by size:
3472 526 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 736 745 736
Size: 9 4049
BseYI C'CCAG_C
Cuts at: 225 371 732 1483 2241 2818 3314 225
Size: 146 361 751 758 577 496 969
Fragments arranged by size:
969 758 751 577 496 361 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 513 513
Size: 4058
BsiEI CG_ry'CG
Cuts at: 104 471 1600 1646 2854 104
Size: 367 1129 46 1208 1308
Fragments arranged by size:
1308 1208 1129 367 46
BsiHKAI G_wGCw'C
Cuts at: 512 863 1072 1850 2040 2832 512
Size: 351 209 778 190 792 1738
Fragments arranged by size:
1738 792 778 351 209 190
Bsp1286I G_dGCh'C
Cuts at: 303 512 863 1072 1683 1776 1850 2040 2102
Size: 209 351 209 611 93 74 190 62
Cuts at: 2102 2832 303
Size: 730 1529
Fragments arranged by size:
1529 730 611 351 209 209 190 93
74 62
BspEI T'CCGG_A
Cuts at: 912 3278 912
Size: 2366 1692
BspHI T'CATG_A
Cuts at: 512 3234 512
Size: 2722 1336
BspMI ACCTGCnnnn'nnnn_
Cuts at: 242 368 541 1108 1322 1624 2005 242
Size: 126 173 567 214 302 381 2295
Fragments arranged by size:
2295 567 381 302 214 173 126
BsrBI CCG'CTC
Cuts at: 50 2350 2404 3888 4053 50
Size: 2300 54 1484 165 55
Fragments arranged by size:
2300 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1970 1970
Size: 4058
BsrFI r'CCGG_y
Cuts at: 2056 2237 2446 2056
Size: 181 209 3668
Fragments arranged by size:
3668 209 181
BssSI C'ACGA_G
Cuts at: 2329 2687 2329
Size: 358 3700
BstAPI GCAn_nnn'nTGC
Cuts at: 1435 1435
Size: 4058
BstBI TT'CG_AA
Cuts at: 2419 2419
Size: 4058
BstXI CCAn_nnnn'nTGG
Cuts at: 705 705
Size: 4058
BstYI r'GATC_y
Cuts at: 1 317 626 791 1083 1550 1573 1908 2154
Size: 316 309 165 292 467 23 335 246
Cuts at: 2154 3166 3252 3264 3610 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 316 309 292
246 165 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 985 985
Size: 4058
BtgI C'CryG_G
Cuts at: 329 329
Size: 4058
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 366 2008 2189 2352 366
Size: 1642 181 163 2072
Fragments arranged by size:
2072 1642 181 163
BtsI GCAGTG_nn'
Cuts at: 1301 1301
Size: 4058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 641 676 641
Size: 35 4023
DraI TTT'AAA
Cuts at: 1057 1057
Size: 4058
DrdI GACnn_nn'nnGTC
Cuts at: 1764 2622 1764
Size: 858 3200
EaeI y'GGCC_r
Cuts at: 245 332 353 479 590 695 776 1643 1817
Size: 87 21 126 111 105 81 867 174
Cuts at: 1817 2208 2235 3580 245
Size: 391 27 1345 723
Fragments arranged by size:
1345 867 723 391 174 126 111 105
87 81 27 21
EagI C'GGCC_G
Cuts at: 1643 1643
Size: 4058
EarI CTCTTCn'nnn_
Cuts at: 193 295 2081 2291 193
Size: 102 1786 210 1960
Fragments arranged by size:
1960 1786 210 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2266 2576 2722 3868 2266
Size: 310 146 1146 2456
Fragments arranged by size:
2456 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1070 1070
Size: 4058
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 276 405 824 910 963 1021 1883 2315
Size: 116 129 419 86 53 58 862 432
Cuts at: 2315 3062 3330 160
Size: 747 268 888
Fragments arranged by size:
888 862 747 432 419 268 129 116
86 58 53
EcoO109I rG'GnC_Cy
Cuts at: 901 1197 901
Size: 296 3762
EcoRI G'AATT_C
Cuts at: 139 1062 139
Size: 923 3135
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1041 2077 1041
Size: 1036 3022
FspI TGC'GCA
Cuts at: 1839 1839
Size: 4058
HaeII r_GCGC'y
Cuts at: 378 1492 1740 2762 3741 378
Size: 1114 248 1022 979 695
Fragments arranged by size:
1114 1022 979 695 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 1900 1930 1932 1962 349
Size: 32 1519 30 2 30 2445
Fragments arranged by size:
2445 1519 32 30 30 2
HincII GTy'rAC
Cuts at: 1097 1097
Size: 4058
KasI G'GCGC_C
Cuts at: 1488 1736 1488
Size: 248 3810
KpnI G_GTAC'C
Cuts at: 1078 1078
Size: 4058
MluI A'CGCG_T
Cuts at: 3491 3491
Size: 4058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 790 2729 2913 790
Size: 1939 184 1935
Fragments arranged by size:
1939 1935 184
MscI TGG'CCA
Cuts at: 247 334 481 697 1819 247
Size: 87 147 216 1122 2486
Fragments arranged by size:
2486 1122 216 147 87
MslI CAynn'nnrTG
Cuts at: 130 280 457 574 850 928 2047 2174 130
Size: 150 177 117 276 78 1119 127 2014
Fragments arranged by size:
2014 1119 276 177 150 127 117 78
MspA1I CmG'CkG
Cuts at: 180 1169 1483 1843 2856 3101 180
Size: 989 314 360 1013 245 1137
Fragments arranged by size:
1137 1013 989 360 314 245
NaeI GCC'GGC
Cuts at: 2239 2239
Size: 4058
NarI GG'CG_CC
Cuts at: 1489 1737 1489
Size: 248 3810
NgoMIV G'CCGG_C
Cuts at: 2237 2237
Size: 4058
NspI r_CATG'y
Cuts at: 279 351 1111 1611 2518 279
Size: 72 760 500 907 1819
Fragments arranged by size:
1819 907 760 500 72
PciI A'CATG_T
Cuts at: 2514 2514
Size: 4058
PflMI CCAn_nnn'nTGG
Cuts at: 254 254
Size: 4058
PmeI GTTT'AAAC
Cuts at: 1057 1057
Size: 4058
PmlI CAC'GTG
Cuts at: 648 648
Size: 4058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3197 3229 3197
Size: 32 4026
PpuMI rG'GwC_Cy
Cuts at: 901 901
Size: 4058
PstI C_TGCA'G
Cuts at: 1105 1790 1105
Size: 685 3373
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4058
PvuII CAG'CTG
Cuts at: 180 1483 1843 180
Size: 1303 360 2395
Fragments arranged by size:
2395 1303 360
RsrII CG'GwC_CG
Cuts at: 2253 2253
Size: 4058
SacI G_AGCT'C
Cuts at: 1072 1072
Size: 4058
SalI G'TCGA_C
Cuts at: 1095 1095
Size: 4058
SapI GCTCTTCn'nnn_
Cuts at: 193 2081 2291 193
Size: 1888 210 1960
Fragments arranged by size:
1960 1888 210
SbfI CC_TGCA'GG
Cuts at: 1105 1105
Size: 4058
ScaI AGT'ACT
Cuts at: 3575 3575
Size: 4058
SfcI C'TryA_G
Cuts at: 33 1101 1786 2779 2970 33
Size: 1068 685 993 191 1121
Fragments arranged by size:
1121 1068 993 685 191
SfoI GGC'GCC
Cuts at: 1490 1738 1490
Size: 248 3810
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4058
SmaI CCC'GGG
Cuts at: 1080 1080
Size: 4058
SmlI C'TyrA_G
Cuts at: 1213 2128 2620 2882 1213
Size: 915 492 262 2389
Fragments arranged by size:
2389 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4058
SphI G_CATG'C
Cuts at: 351 1111 1611 351
Size: 760 500 2798
Fragments arranged by size:
2798 760 500
StuI AGG'CCT
Cuts at: 820 820
Size: 4058
StyI C'CwwG_G
Cuts at: 1192 1546 1192
Size: 354 3704
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 385 451 1641 1774 2233 2442 385
Size: 66 1190 133 459 209 2001
Fragments arranged by size:
2001 1190 459 209 133 66
TatI w'GTAC_w
Cuts at: 3573 3573
Size: 4058
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 668 700 3575 3607 668
Size: 32 2875 32 1119
Fragments arranged by size:
2875 1119 32 32
Tth111I GACn'n_nGTC
Cuts at: 1855 1855
Size: 4058
XbaI T'CTAG_A
Cuts at: 1089 1089
Size: 4058
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 387 1553 387
Size: 1166 2892
XmaI C'CCGG_G
Cuts at: 1078 1078
Size: 4058
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PciI PflMI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AlfI AloI ApaI AscI AvrII BaeI
BfrBI BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI