Restriction map for
pF1KE0856
(Circular) (Six-base) MAPSORT of: pF1KE0856.seq Check: 3650 from: 1 to: 4061
pF1KE0856 4061 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4061
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1886 2318 3065 3333 1024
Size: 862 432 747 268 1752
Fragments arranged by size:
1752 862 747 432 268
AflIII A'CryG_T
Cuts at: 1413 2517 3494 1413
Size: 1104 977 1980
Fragments arranged by size:
1980 1104 977
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AleI CACnn'nnGTG
Cuts at: 391 853 391
Size: 462 3599
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 257 289 257
Size: 32 4029
AlwNI CAG_nnn'CTG
Cuts at: 180 1366 2933 180
Size: 1186 1567 1308
Fragments arranged by size:
1567 1308 1186
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 139 1065 3414 3670 139
Size: 926 2349 256 530
Fragments arranged by size:
2349 926 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 1081 1081
Size: 4061
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 271 304 821 854 271
Size: 33 517 33 3478
Fragments arranged by size:
3478 517 33 33
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 1077 1491 1739 1774 1077
Size: 414 248 35 3364
Fragments arranged by size:
3364 414 248 35
BanII G_rGCy'C
Cuts at: 1075 2105 1075
Size: 1030 3031
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BbsI GAAGACnn'nnnn_
Cuts at: 762 945 762
Size: 183 3878
BbvCI CC'TCA_GC
Cuts at: 806 967 806
Size: 161 3900
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4027
BciVI GTATCCnnnnn_n'
Cuts at: 849 1959 2720 4061 849
Size: 1110 761 1341 849
Fragments arranged by size:
1341 1110 849 761
BclI T'GATC_A
Cuts at: 471 1581 471
Size: 1110 2951
BglI GCCn_nnn'nGGC
Cuts at: 934 934
Size: 4061
BglII A'GATC_T
Cuts at: 1576 3613 1576
Size: 2037 2024
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 306 537 1686 1779 2835 306
Size: 231 1149 93 1056 1532
Fragments arranged by size:
1532 1149 1056 231 93
BmrI ACTGGGnnnn_n'
Cuts at: 1273 1354 1689 3833 1273
Size: 81 335 2144 1501
Fragments arranged by size:
2144 1501 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
Bpu10I CC'TnA_GC
Cuts at: 806 967 806
Size: 161 3900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 810 1237 2116 2608 2906 810
Size: 427 879 492 298 1965
Fragments arranged by size:
1965 879 492 427 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 480 1414 480
Size: 934 3127
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1250 1771 2449 2723 2870 3281 1250
Size: 521 678 274 147 411 2030
Fragments arranged by size:
2030 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 257 287 257
Size: 30 4031
BseYI C'CCAG_C
Cuts at: 170 1486 2244 2821 3317 170
Size: 1316 758 577 496 914
Fragments arranged by size:
1316 914 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1075 1853 2043 2835 1075
Size: 778 190 792 2301
Fragments arranged by size:
2301 792 778 190
BsmI GAATG_Cn'
Cuts at: 1014 1014
Size: 4061
Bsp1286I G_dGCh'C
Cuts at: 306 537 1075 1686 1779 1853 2043 2105 2835
Size: 231 538 611 93 74 190 62 730
Cuts at: 2835 306
Size: 1532
Fragments arranged by size:
1532 730 611 538 231 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1111 1325 1627 2008 1111
Size: 214 302 381 3164
Fragments arranged by size:
3164 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 223 1973 223
Size: 1750 2311
BsrFI r'CCGG_y
Cuts at: 812 2059 2240 2449 812
Size: 1247 181 209 2424
Fragments arranged by size:
2424 1247 209 181
BsrGI T'GTAC_A
Cuts at: 917 917
Size: 4061
BssSI C'ACGA_G
Cuts at: 2332 2690 2332
Size: 358 3703
BstAPI GCAn_nnn'nTGC
Cuts at: 180 1438 180
Size: 1258 2803
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstYI r'GATC_y
Cuts at: 1 1086 1553 1576 1911 2157 3169 3255 3267
Size: 1085 467 23 335 246 1012 86 12
Cuts at: 3267 3613 1
Size: 346 449
Fragments arranged by size:
1085 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 525 525
Size: 4061
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 496 1304 496
Size: 808 3253
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DraIII CAC_nnn'GTG
Cuts at: 480 480
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 248 935 1646 1820 2211 2238 3583 248
Size: 687 711 174 391 27 1345 726
Fragments arranged by size:
1345 726 711 687 391 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 298 373 2084 2294 298
Size: 75 1711 210 2065
Fragments arranged by size:
2065 1711 210 75
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1024 1886 2318 3065 3333 1024
Size: 862 432 747 268 1752
Fragments arranged by size:
1752 862 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 903 1200 903
Size: 297 3764
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 4061
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 1495 1743 2765 3744 1495
Size: 248 1022 979 1812
Fragments arranged by size:
1812 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1903 1933 1935 1965 1903
Size: 30 2 30 3999
Fragments arranged by size:
3999 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2732 2916 2732
Size: 184 3877
MscI TGG'CCA
Cuts at: 250 937 1822 250
Size: 687 885 2489
Fragments arranged by size:
2489 885 687
MslI CAynn'nnrTG
Cuts at: 283 391 409 460 514 577 706 853 2050
Size: 108 18 51 54 63 129 147 1197
Cuts at: 2050 2177 283
Size: 127 2167
Fragments arranged by size:
2167 1197 147 129 127 108 63 54
51 18
MspA1I CmG'CkG
Cuts at: 1172 1486 1846 2859 3104 1172
Size: 314 360 1013 245 2129
Fragments arranged by size:
2129 1013 360 314 245
NaeI GCC'GGC
Cuts at: 814 2242 814
Size: 1428 2633
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NgoMIV G'CCGG_C
Cuts at: 812 2240 812
Size: 1428 2633
NspI r_CATG'y
Cuts at: 354 513 1114 1614 2521 354
Size: 159 601 500 907 1894
Fragments arranged by size:
1894 907 601 500 159
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PflMI CCAn_nnn'nTGG
Cuts at: 257 543 257
Size: 286 3775
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 257 289 310 342 3200 3232 257
Size: 32 21 32 2858 32 1086
Fragments arranged by size:
2858 1086 32 32 32 21
PstI C_TGCA'G
Cuts at: 1108 1793 1108
Size: 685 3376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 1486 1846 1486
Size: 360 3701
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 373 2084 2294 373
Size: 1711 210 2140
Fragments arranged by size:
2140 1711 210
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 123 867 1104 1789 2782 2973 33
Size: 90 744 237 685 993 191 1121
Fragments arranged by size:
1121 993 744 685 237 191 90
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 789 1216 2131 2623 2885 789
Size: 427 915 492 262 1965
Fragments arranged by size:
1965 915 492 427 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 354 1114 1614 354
Size: 760 500 2801
Fragments arranged by size:
2801 760 500
StyI C'CwwG_G
Cuts at: 899 1195 1549 899
Size: 296 354 3411
Fragments arranged by size:
3411 354 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 297 388 509 558 694 1644 1777 2236 2445
Size: 91 121 49 136 950 133 459 209
Cuts at: 2445 297
Size: 1913
Fragments arranged by size:
1913 950 459 209 136 133 121 91
49
TatI w'GTAC_w
Cuts at: 917 3576 917
Size: 2659 1402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4029
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 390 1556 390
Size: 1166 2895
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BfrBI BmgBI BmtI BpmI BseRI BsgI BsiWI
BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI