Restriction map for
pF1KE0855
(Circular) (Six-base) MAPSORT of: pF1KE0855.seq Check: 8970 from: 1 to: 4046
pF1KE0855 4046 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1084 1084
Size: 4046
Acc65I G'GTAC_C
Cuts at: 1062 1062
Size: 4046
AclI AA'CG_TT
Cuts at: 3886 3886
Size: 4046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1871 2303 3050 3318 1871
Size: 432 747 268 2599
Fragments arranged by size:
2599 747 432 268
AflIII A'CryG_T
Cuts at: 311 1398 2502 3479 311
Size: 1087 1104 977 878
Fragments arranged by size:
1104 1087 977 878
AgeI A'CCGG_T
Cuts at: 2434 2434
Size: 4046
AlwNI CAG_nnn'CTG
Cuts at: 1351 2918 1351
Size: 1567 2479
ApaLI G'TGCA_C
Cuts at: 2816 2816
Size: 4046
ApoI r'AATT_y
Cuts at: 1050 3399 3655 1050
Size: 2349 256 1441
Fragments arranged by size:
2349 1441 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4046
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
AvaI C'yCGr_G
Cuts at: 726 1066 726
Size: 340 3706
BamHI G'GATC_C
Cuts at: 785 1071 785
Size: 286 3760
BanI G'GyrC_C
Cuts at: 333 1062 1476 1724 1759 333
Size: 729 414 248 35 2620
Fragments arranged by size:
2620 729 414 248 35
BanII G_rGCy'C
Cuts at: 1060 2090 1060
Size: 1030 3016
BbeI G_GCGC'C
Cuts at: 1480 1728 1480
Size: 248 3798
BbsI GAAGACnn'nnnn_
Cuts at: 183 183
Size: 4046
BbvCI CC'TCA_GC
Cuts at: 681 681
Size: 4046
BciVI GTATCCnnnnn_n'
Cuts at: 1944 2705 4046 1944
Size: 761 1341 1944
Fragments arranged by size:
1944 1341 761
BclI T'GATC_A
Cuts at: 245 1566 245
Size: 1321 2725
BglI GCCn_nnn'nGGC
Cuts at: 535 535
Size: 4046
BglII A'GATC_T
Cuts at: 1561 3598 1561
Size: 2037 2009
BlpI GC'TnA_GC
Cuts at: 401 1158 401
Size: 757 3289
Bme1580I G_kGCm'C
Cuts at: 338 531 1671 1764 2820 338
Size: 193 1140 93 1056 1564
Fragments arranged by size:
1564 1140 1056 193 93
BmrI ACTGGGnnnn_n'
Cuts at: 524 1258 1339 1674 3818 524
Size: 734 81 335 2144 752
Fragments arranged by size:
2144 752 734 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4046
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 879
Size: 4046
Bpu10I CC'TnA_GC
Cuts at: 493 681 1031 493
Size: 188 350 3508
Fragments arranged by size:
3508 350 188
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1222 2101 2593 2891 1222
Size: 879 492 298 2377
Fragments arranged by size:
2377 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3750 3750
Size: 4046
BsaAI yAC'GTr
Cuts at: 207 1399 207
Size: 1192 2854
BsaBI GATnn'nnATC
Cuts at: 6 697 1560 3597 6
Size: 691 863 2037 455
Fragments arranged by size:
2037 863 691 455
BsaHI Gr'CG_yC
Cuts at: 173 902 1477 1725 2427 3923 173
Size: 729 575 248 702 1496 296
Fragments arranged by size:
1496 729 702 575 296 248
BsaWI w'CCGG_w
Cuts at: 1235 1756 2434 2708 2855 3266 1235
Size: 521 678 274 147 411 2015
Fragments arranged by size:
2015 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 927 957 927
Size: 30 4016
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 935 220
Size: 715 3331
BseYI C'CCAG_C
Cuts at: 219 1471 2229 2806 3302 219
Size: 1252 758 577 496 963
Fragments arranged by size:
1252 963 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 550 692 550
Size: 142 3904
BsiEI CG_ry'CG
Cuts at: 104 1588 1634 2842 104
Size: 1484 46 1208 1308
Fragments arranged by size:
1484 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 212 433 1060 1838 2028 2820 212
Size: 221 627 778 190 792 1438
Fragments arranged by size:
1438 792 778 627 221 190
Bsp1286I G_dGCh'C
Cuts at: 212 338 433 531 1060 1671 1764 1838 2028
Size: 126 95 98 529 611 93 74 190
Cuts at: 2028 2090 2820 212
Size: 62 730 1438
Fragments arranged by size:
1438 730 611 529 190 126 98 95
93 74 62
BspEI T'CCGG_A
Cuts at: 3266 3266
Size: 4046
BspHI T'CATG_A
Cuts at: 3222 3222
Size: 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1096 1310 1612 1993 1096
Size: 214 302 381 3149
Fragments arranged by size:
3149 381 302 214
BsrBI CCG'CTC
Cuts at: 50 492 2338 2392 3876 4041 50
Size: 442 1846 54 1484 165 55
Fragments arranged by size:
1846 1484 442 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1958 1958
Size: 4046
BsrFI r'CCGG_y
Cuts at: 541 2044 2225 2434 541
Size: 1503 181 209 2153
Fragments arranged by size:
2153 1503 209 181
BsrGI T'GTAC_A
Cuts at: 965 965
Size: 4046
BssSI C'ACGA_G
Cuts at: 522 2317 2675 522
Size: 1795 358 1893
Fragments arranged by size:
1893 1795 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1423 1423
Size: 4046
BstBI TT'CG_AA
Cuts at: 2407 2407
Size: 4046
BstXI CCAn_nnnn'nTGG
Cuts at: 693 693
Size: 4046
BstYI r'GATC_y
Cuts at: 1 623 785 1071 1538 1561 1896 2142 3154
Size: 622 162 286 467 23 335 246 1012
Cuts at: 3154 3240 3252 3598 1
Size: 86 12 346 449
Fragments arranged by size:
1012 622 467 449 346 335 286 246
162 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 883 883
Size: 4046
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1996 2177 2340 1996
Size: 181 163 3702
Fragments arranged by size:
3702 181 163
BtsI GCAGTG_nn'
Cuts at: 1289 1289
Size: 4046
DraI TTT'AAA
Cuts at: 1045 1045
Size: 4046
DraIII CAC_nnn'GTG
Cuts at: 437 437
Size: 4046
DrdI GACnn_nn'nnGTC
Cuts at: 357 1752 2610 357
Size: 1395 858 1793
Fragments arranged by size:
1793 1395 858
EaeI y'GGCC_r
Cuts at: 476 536 920 1631 1805 2196 2223 3568 476
Size: 60 384 711 174 391 27 1345 954
Fragments arranged by size:
1345 954 711 391 384 174 60 27
EagI C'GGCC_G
Cuts at: 1631 1631
Size: 4046
EarI CTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2254 2564 2710 3856 2254
Size: 310 146 1146 2444
Fragments arranged by size:
2444 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1058 1058
Size: 4046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 879 1871 2303 3050 3318 879
Size: 992 432 747 268 1607
Fragments arranged by size:
1607 992 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1185 1185
Size: 4046
EcoRI G'AATT_C
Cuts at: 1050 1050
Size: 4046
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2065 2065
Size: 4046
FspI TGC'GCA
Cuts at: 1827 1827
Size: 4046
HaeII r_GCGC'y
Cuts at: 522 1480 1728 2750 3729 522
Size: 958 248 1022 979 839
Fragments arranged by size:
1022 979 958 839 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1888 1918 1920 1950 1888
Size: 30 2 30 3984
Fragments arranged by size:
3984 30 30 2
HincII GTy'rAC
Cuts at: 1085 1085
Size: 4046
KasI G'GCGC_C
Cuts at: 1476 1724 1476
Size: 248 3798
KpnI G_GTAC'C
Cuts at: 1066 1066
Size: 4046
MluI A'CGCG_T
Cuts at: 3479 3479
Size: 4046
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 137 2717 2901 137
Size: 2580 184 1282
Fragments arranged by size:
2580 1282 184
MscI TGG'CCA
Cuts at: 478 538 1807 478
Size: 60 1269 2717
Fragments arranged by size:
2717 1269 60
MslI CAynn'nnrTG
Cuts at: 274 310 454 505 703 2035 2162 274
Size: 36 144 51 198 1332 127 2158
Fragments arranged by size:
2158 1332 198 144 127 51 36
MspA1I CmG'CkG
Cuts at: 392 1157 1471 1831 2844 3089 392
Size: 765 314 360 1013 245 1349
Fragments arranged by size:
1349 1013 765 360 314 245
NaeI GCC'GGC
Cuts at: 2227 2227
Size: 4046
NarI GG'CG_CC
Cuts at: 1477 1725 1477
Size: 248 3798
NgoMIV G'CCGG_C
Cuts at: 2225 2225
Size: 4046
NspI r_CATG'y
Cuts at: 315 1099 1599 2506 315
Size: 784 500 907 1855
Fragments arranged by size:
1855 907 784 500
PasI CC'CwG_GG
Cuts at: 163 163
Size: 4046
PciI A'CATG_T
Cuts at: 311 2502 311
Size: 2191 1855
PflMI CCAn_nnn'nTGG
Cuts at: 184 423 184
Size: 239 3807
PmeI GTTT'AAAC
Cuts at: 1045 1045
Size: 4046
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3185 3217 3185
Size: 32 4014
PstI C_TGCA'G
Cuts at: 1093 1778 1093
Size: 685 3361
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4046
PvuII CAG'CTG
Cuts at: 392 1471 1831 392
Size: 1079 360 2607
Fragments arranged by size:
2607 1079 360
RsrII CG'GwC_CG
Cuts at: 2241 2241
Size: 4046
SacI G_AGCT'C
Cuts at: 1060 1060
Size: 4046
SalI G'TCGA_C
Cuts at: 1083 1083
Size: 4046
SapI GCTCTTCn'nnn_
Cuts at: 2069 2279 2069
Size: 210 3836
SbfI CC_TGCA'GG
Cuts at: 1093 1093
Size: 4046
ScaI AGT'ACT
Cuts at: 3563 3563
Size: 4046
SfcI C'TryA_G
Cuts at: 33 1089 1774 2767 2958 33
Size: 1056 685 993 191 1121
Fragments arranged by size:
1121 1056 993 685 191
SfoI GGC'GCC
Cuts at: 1478 1726 1478
Size: 248 3798
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4046
SmaI CCC'GGG
Cuts at: 1068 1068
Size: 4046
SmlI C'TyrA_G
Cuts at: 1201 2116 2608 2870 1201
Size: 915 492 262 2377
Fragments arranged by size:
2377 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4046
SphI G_CATG'C
Cuts at: 1099 1599 1099
Size: 500 3546
SspI AAT'ATT
Cuts at: 711 711
Size: 4046
StyI C'CwwG_G
Cuts at: 353 1180 1534 353
Size: 827 354 2865
Fragments arranged by size:
2865 827 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1629 1762 2221 2430 1629
Size: 133 459 209 3245
Fragments arranged by size:
3245 459 209 133
TatI w'GTAC_w
Cuts at: 278 965 3561 278
Size: 687 2596 763
Fragments arranged by size:
2596 763 687
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 135 167 638 670 927 959 3563 3595 135
Size: 32 471 32 257 32 2604 32 586
Fragments arranged by size:
2604 586 471 257 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1843 1843
Size: 4046
XbaI T'CTAG_A
Cuts at: 1077 1077
Size: 4046
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 660 1541 660
Size: 881 3165
XmaI C'CCGG_G
Cuts at: 1066 1066
Size: 4046
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I BtgI ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI XmnI ZraI