Restriction map for
pF1KE0854
(Circular) (Six-base) MAPSORT of: pF1KE0854.seq Check: 749 from: 1 to: 4109
pF1KE0854 4109 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 805 805
Size: 4109
AccI GT'mk_AC
Cuts at: 692 1147 692
Size: 455 3654
Acc65I G'GTAC_C
Cuts at: 1125 1125
Size: 4109
AclI AA'CG_TT
Cuts at: 3949 3949
Size: 4109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1934 2366 3113 3381 1934
Size: 432 747 268 2662
Fragments arranged by size:
2662 747 432 268
AflIII A'CryG_T
Cuts at: 889 1461 2565 3542 889
Size: 572 1104 977 1456
Fragments arranged by size:
1456 1104 977 572
AgeI A'CCGG_T
Cuts at: 2497 2497
Size: 4109
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 187 187
Size: 4109
AlwNI CAG_nnn'CTG
Cuts at: 590 1414 2981 590
Size: 824 1567 1718
Fragments arranged by size:
1718 1567 824
ApaLI G'TGCA_C
Cuts at: 2879 2879
Size: 4109
ApoI r'AATT_y
Cuts at: 851 1113 3462 3718 851
Size: 262 2349 256 1242
Fragments arranged by size:
2349 1242 262 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4109
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
AvaI C'yCGr_G
Cuts at: 1129 1129
Size: 4109
BamHI G'GATC_C
Cuts at: 1134 1134
Size: 4109
BanI G'GyrC_C
Cuts at: 610 869 1125 1539 1787 1822 610
Size: 259 256 414 248 35 2897
Fragments arranged by size:
2897 414 259 256 248 35
BanII G_rGCy'C
Cuts at: 302 971 1123 2153 302
Size: 669 152 1030 2258
Fragments arranged by size:
2258 1030 669 152
BbeI G_GCGC'C
Cuts at: 873 1543 1791 873
Size: 670 248 3191
Fragments arranged by size:
3191 670 248
BciVI GTATCCnnnnn_n'
Cuts at: 2007 2768 4109 2007
Size: 761 1341 2007
Fragments arranged by size:
2007 1341 761
BclI T'GATC_A
Cuts at: 1629 1629
Size: 4109
BglI GCCn_nnn'nGGC
Cuts at: 745 745
Size: 4109
BglII A'GATC_T
Cuts at: 1624 3661 1624
Size: 2037 2072
BlpI GC'TnA_GC
Cuts at: 1221 1221
Size: 4109
Bme1580I G_kGCm'C
Cuts at: 932 1734 1827 2883 932
Size: 802 93 1056 2158
Fragments arranged by size:
2158 1056 802 93
BmrI ACTGGGnnnn_n'
Cuts at: 1321 1402 1737 3881 1321
Size: 81 335 2144 1549
Fragments arranged by size:
2144 1549 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 265 2476 265
Size: 2211 1898
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1285 2164 2656 2954 1285
Size: 879 492 298 2440
Fragments arranged by size:
2440 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3813 3813
Size: 4109
BsaAI yAC'GTr
Cuts at: 890 1462 890
Size: 572 3537
BsaBI GATnn'nnATC
Cuts at: 6 1623 3660 6
Size: 1617 2037 455
Fragments arranged by size:
2037 1617 455
BsaHI Gr'CG_yC
Cuts at: 870 1540 1788 2490 3986 870
Size: 670 248 702 1496 993
Fragments arranged by size:
1496 993 702 670 248
BsaWI w'CCGG_w
Cuts at: 1298 1819 2497 2771 2918 3329 1298
Size: 521 678 274 147 411 2078
Fragments arranged by size:
2078 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 268
Size: 4109
BseYI C'CCAG_C
Cuts at: 237 618 1534 2292 2869 3365 237
Size: 381 916 758 577 496 981
Fragments arranged by size:
981 916 758 577 496 381
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 918 1114 205
Size: 713 196 3200
Fragments arranged by size:
3200 713 196
BsiEI CG_ry'CG
Cuts at: 104 1651 1697 2905 104
Size: 1547 46 1208 1308
Fragments arranged by size:
1547 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1123 1901 2091 2883 1123
Size: 778 190 792 2349
Fragments arranged by size:
2349 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 302 932 971 1123 1734 1827 1901 2091 2153
Size: 630 39 152 611 93 74 190 62
Cuts at: 2153 2883 302
Size: 730 1528
Fragments arranged by size:
1528 730 630 611 190 152 93 74
62 39
BspEI T'CCGG_A
Cuts at: 3329 3329
Size: 4109
BspHI T'CATG_A
Cuts at: 575 3285 575
Size: 2710 1399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 956 1159 1373 1675 2056 805
Size: 151 203 214 302 381 2858
Fragments arranged by size:
2858 381 302 214 203 151
BsrBI CCG'CTC
Cuts at: 50 2401 2455 3939 4104 50
Size: 2351 54 1484 165 55
Fragments arranged by size:
2351 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 882 2021 882
Size: 1139 2970
BsrFI r'CCGG_y
Cuts at: 2107 2288 2497 2107
Size: 181 209 3719
Fragments arranged by size:
3719 209 181
BssSI C'ACGA_G
Cuts at: 2380 2738 2380
Size: 358 3751
BstAPI GCAn_nnn'nTGC
Cuts at: 1486 1486
Size: 4109
BstBI TT'CG_AA
Cuts at: 2470 2470
Size: 4109
BstEII G'GTnAC_C
Cuts at: 762 762
Size: 4109
BstXI CCAn_nnnn'nTGG
Cuts at: 414 414
Size: 4109
BstYI r'GATC_y
Cuts at: 1 1134 1601 1624 1959 2205 3217 3303 3315
Size: 1133 467 23 335 246 1012 86 12
Cuts at: 3315 3661 1
Size: 346 449
Fragments arranged by size:
1133 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2059 2240 2403 2059
Size: 181 163 3765
Fragments arranged by size:
3765 181 163
BtsI GCAGTG_nn'
Cuts at: 1352 1352
Size: 4109
DraI TTT'AAA
Cuts at: 1108 1108
Size: 4109
DraIII CAC_nnn'GTG
Cuts at: 1040 1040
Size: 4109
DrdI GACnn_nn'nnGTC
Cuts at: 195 1815 2673 195
Size: 1620 858 1631
Fragments arranged by size:
1631 1620 858
EaeI y'GGCC_r
Cuts at: 746 1694 1868 2259 2286 3631 746
Size: 948 174 391 27 1345 1224
Fragments arranged by size:
1345 1224 948 391 174 27
EagI C'GGCC_G
Cuts at: 1694 1694
Size: 4109
EarI CTCTTCn'nnn_
Cuts at: 256 2132 2342 256
Size: 1876 210 2023
Fragments arranged by size:
2023 1876 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2317 2627 2773 3919 2317
Size: 310 146 1146 2507
Fragments arranged by size:
2507 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1121 1121
Size: 4109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1934 2366 3113 3381 1934
Size: 432 747 268 2662
Fragments arranged by size:
2662 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1248 1248
Size: 4109
EcoRI G'AATT_C
Cuts at: 851 1113 851
Size: 262 3847
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2128 2128
Size: 4109
FspI TGC'GCA
Cuts at: 1890 1890
Size: 4109
HaeII r_GCGC'y
Cuts at: 247 873 1543 1791 2813 3792 247
Size: 626 670 248 1022 979 564
Fragments arranged by size:
1022 979 670 626 564 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 614 646 1951 1981 1983 2013 614
Size: 32 1305 30 2 30 2710
Fragments arranged by size:
2710 1305 32 30 30 2
HincII GTy'rAC
Cuts at: 693 1148 693
Size: 455 3654
KasI G'GCGC_C
Cuts at: 869 1539 1787 869
Size: 670 248 3191
Fragments arranged by size:
3191 670 248
KpnI G_GTAC'C
Cuts at: 1129 1129
Size: 4109
MluI A'CGCG_T
Cuts at: 3542 3542
Size: 4109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 492 2780 2964 492
Size: 2288 184 1637
Fragments arranged by size:
2288 1637 184
MscI TGG'CCA
Cuts at: 1870 1870
Size: 4109
MslI CAynn'nnrTG
Cuts at: 211 520 2098 2225 211
Size: 309 1578 127 2095
Fragments arranged by size:
2095 1578 309 127
MspA1I CmG'CkG
Cuts at: 237 1220 1534 1894 2907 3152 237
Size: 983 314 360 1013 245 1194
Fragments arranged by size:
1194 1013 983 360 314 245
NaeI GCC'GGC
Cuts at: 2290 2290
Size: 4109
NarI GG'CG_CC
Cuts at: 870 1540 1788 870
Size: 670 248 3191
Fragments arranged by size:
3191 670 248
NgoMIV G'CCGG_C
Cuts at: 2288 2288
Size: 4109
NspI r_CATG'y
Cuts at: 1162 1662 2569 1162
Size: 500 907 2702
Fragments arranged by size:
2702 907 500
PciI A'CATG_T
Cuts at: 2565 2565
Size: 4109
PflMI CCAn_nnn'nTGG
Cuts at: 489 489
Size: 4109
PmeI GTTT'AAAC
Cuts at: 1108 1108
Size: 4109
PmlI CAC'GTG
Cuts at: 890 890
Size: 4109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3248 3280 3248
Size: 32 4077
PstI C_TGCA'G
Cuts at: 1156 1841 1156
Size: 685 3424
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4109
PvuII CAG'CTG
Cuts at: 237 1534 1894 237
Size: 1297 360 2452
Fragments arranged by size:
2452 1297 360
RsrII CG'GwC_CG
Cuts at: 2304 2304
Size: 4109
SacI G_AGCT'C
Cuts at: 1123 1123
Size: 4109
SalI G'TCGA_C
Cuts at: 691 1146 691
Size: 455 3654
SapI GCTCTTCn'nnn_
Cuts at: 2132 2342 2132
Size: 210 3899
SbfI CC_TGCA'GG
Cuts at: 1156 1156
Size: 4109
ScaI AGT'ACT
Cuts at: 3626 3626
Size: 4109
SfcI C'TryA_G
Cuts at: 33 1152 1837 2830 3021 33
Size: 1119 685 993 191 1121
Fragments arranged by size:
1121 1119 993 685 191
SfoI GGC'GCC
Cuts at: 871 1541 1789 871
Size: 670 248 3191
Fragments arranged by size:
3191 670 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4109
SmaI CCC'GGG
Cuts at: 1131 1131
Size: 4109
SmlI C'TyrA_G
Cuts at: 1264 2179 2671 2933 1264
Size: 915 492 262 2440
Fragments arranged by size:
2440 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4109
SphI G_CATG'C
Cuts at: 1162 1662 1162
Size: 500 3609
StyI C'CwwG_G
Cuts at: 1243 1597 1243
Size: 354 3755
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 242 1692 1825 2284 2493 242
Size: 1450 133 459 209 1858
Fragments arranged by size:
1858 1450 459 209 133
TatI w'GTAC_w
Cuts at: 496 3624 496
Size: 3128 981
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4077
Tth111I GACn'n_nGTC
Cuts at: 1906 1906
Size: 4109
XbaI T'CTAG_A
Cuts at: 1140 1140
Size: 4109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 756 1604 756
Size: 848 3261
XmaI C'CCGG_G
Cuts at: 1129 1129
Size: 4109
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PflMI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PfoI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI