Restriction map for
pF1KE0853
(Circular) (Six-base) MAPSORT of: pF1KE0853.seq Check: 9014 from: 1 to: 4043
pF1KE0853 4043 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1024 1024
Size: 4043
AatII G_ACGT'C
Cuts at: 731 731
Size: 4043
AccI GT'mk_AC
Cuts at: 872 1081 872
Size: 209 3834
Acc65I G'GTAC_C
Cuts at: 488 1059 488
Size: 571 3472
AclI AA'CG_TT
Cuts at: 3883 3883
Size: 4043
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 820 1039 1868 2300 3047 3315 820
Size: 219 829 432 747 268 1548
Fragments arranged by size:
1548 829 747 432 268 219
AflIII A'CryG_T
Cuts at: 332 1395 2499 3476 332
Size: 1063 1104 977 899
Fragments arranged by size:
1104 1063 977 899
AgeI A'CCGG_T
Cuts at: 2431 2431
Size: 4043
AlwNI CAG_nnn'CTG
Cuts at: 510 940 1348 2915 510
Size: 430 408 1567 1638
Fragments arranged by size:
1638 1567 430 408
ApaI G_GGCC'C
Cuts at: 635 635
Size: 4043
ApaLI G'TGCA_C
Cuts at: 2813 2813
Size: 4043
ApoI r'AATT_y
Cuts at: 1047 3396 3652 1047
Size: 2349 256 1438
Fragments arranged by size:
2349 1438 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4043
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
AvaI C'yCGr_G
Cuts at: 1063 1063
Size: 4043
BamHI G'GATC_C
Cuts at: 1068 1068
Size: 4043
BanI G'GyrC_C
Cuts at: 488 824 1059 1473 1721 1756 488
Size: 336 235 414 248 35 2775
Fragments arranged by size:
2775 414 336 248 235 35
BanII G_rGCy'C
Cuts at: 635 1057 2087 635
Size: 422 1030 2591
Fragments arranged by size:
2591 1030 422
BbeI G_GCGC'C
Cuts at: 828 1477 1725 828
Size: 649 248 3146
Fragments arranged by size:
3146 649 248
BbsI GAAGACnn'nnnn_
Cuts at: 201 201
Size: 4043
BciVI GTATCCnnnnn_n'
Cuts at: 1941 2702 4043 1941
Size: 761 1341 1941
Fragments arranged by size:
1941 1341 761
BclI T'GATC_A
Cuts at: 1563 1563
Size: 4043
BfrBI ATG'CAT
Cuts at: 585 585
Size: 4043
BglII A'GATC_T
Cuts at: 1558 3595 1558
Size: 2037 2006
BlpI GC'TnA_GC
Cuts at: 1155 1155
Size: 4043
Bme1580I G_kGCm'C
Cuts at: 635 1668 1761 2817 635
Size: 1033 93 1056 1861
Fragments arranged by size:
1861 1056 1033 93
BmrI ACTGGGnnnn_n'
Cuts at: 447 514 1255 1336 1671 3815 447
Size: 67 741 81 335 2144 675
Fragments arranged by size:
2144 741 675 335 81 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 581
Size: 4043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1219 2098 2590 2888 1219
Size: 879 492 298 2374
Fragments arranged by size:
2374 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3747 3747
Size: 4043
BsaAI yAC'GTr
Cuts at: 1396 1396
Size: 4043
BsaBI GATnn'nnATC
Cuts at: 6 1557 3594 6
Size: 1551 2037 455
Fragments arranged by size:
2037 1551 455
BsaHI Gr'CG_yC
Cuts at: 728 825 1474 1722 2424 3920 728
Size: 97 649 248 702 1496 851
Fragments arranged by size:
1496 851 702 649 248 97
BsaWI w'CCGG_w
Cuts at: 1232 1753 2431 2705 2852 3263 1232
Size: 521 678 274 147 411 2012
Fragments arranged by size:
2012 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 554 584 554
Size: 30 4013
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 187 333 187
Size: 146 3897
BseYI C'CCAG_C
Cuts at: 237 1468 2226 2803 3299 237
Size: 1231 758 577 496 981
Fragments arranged by size:
1231 981 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 496 496
Size: 4043
BsiEI CG_ry'CG
Cuts at: 104 1585 1631 2839 104
Size: 1481 46 1208 1308
Fragments arranged by size:
1481 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 291 320 527 1057 1835 2025 2817 291
Size: 29 207 530 778 190 792 1517
Fragments arranged by size:
1517 792 778 530 207 190 29
Bsp1286I G_dGCh'C
Cuts at: 291 320 527 635 1057 1668 1761 1835 2025
Size: 29 207 108 422 611 93 74 190
Cuts at: 2025 2087 2817 291
Size: 62 730 1517
Fragments arranged by size:
1517 730 611 422 207 190 108 93
74 62 29
BspEI T'CCGG_A
Cuts at: 3263 3263
Size: 4043
BspHI T'CATG_A
Cuts at: 527 3219 527
Size: 2692 1351
BspMI ACCTGCnnnn'nnnn_
Cuts at: 254 1024 1093 1307 1609 1990 254
Size: 770 69 214 302 381 2307
Fragments arranged by size:
2307 770 381 302 214 69
BsrBI CCG'CTC
Cuts at: 50 2335 2389 3873 4038 50
Size: 2285 54 1484 165 55
Fragments arranged by size:
2285 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1955 1955
Size: 4043
BsrFI r'CCGG_y
Cuts at: 574 2041 2222 2431 574
Size: 1467 181 209 2186
Fragments arranged by size:
2186 1467 209 181
BsrGI T'GTAC_A
Cuts at: 299 299
Size: 4043
BssSI C'ACGA_G
Cuts at: 2314 2672 2314
Size: 358 3685
BstAPI GCAn_nnn'nTGC
Cuts at: 455 1420 455
Size: 965 3078
BstBI TT'CG_AA
Cuts at: 2404 2404
Size: 4043
BstXI CCAn_nnnn'nTGG
Cuts at: 642 642
Size: 4043
BstYI r'GATC_y
Cuts at: 1 1068 1535 1558 1893 2139 3151 3237 3249
Size: 1067 467 23 335 246 1012 86 12
Cuts at: 3249 3595 1
Size: 346 449
Fragments arranged by size:
1067 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 901 901
Size: 4043
BtgI C'CryG_G
Cuts at: 107 140 107
Size: 33 4010
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 113 1993 2174 2337 113
Size: 1880 181 163 1819
Fragments arranged by size:
1880 1819 181 163
BtsI GCAGTG_nn'
Cuts at: 1286 1286
Size: 4043
DraI TTT'AAA
Cuts at: 1042 1042
Size: 4043
DraIII CAC_nnn'GTG
Cuts at: 266 455 266
Size: 189 3854
DrdI GACnn_nn'nnGTC
Cuts at: 700 1749 2607 700
Size: 1049 858 2136
Fragments arranged by size:
2136 1049 858
EaeI y'GGCC_r
Cuts at: 740 1628 1802 2193 2220 3565 740
Size: 888 174 391 27 1345 1218
Fragments arranged by size:
1345 1218 888 391 174 27
EagI C'GGCC_G
Cuts at: 1628 1628
Size: 4043
EarI CTCTTCn'nnn_
Cuts at: 205 1015 2066 2276 205
Size: 810 1051 210 1972
Fragments arranged by size:
1972 1051 810 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 604 2251 2561 2707 3853 604
Size: 1647 310 146 1146 794
Fragments arranged by size:
1647 1146 794 310 146
EcoICRI GAG'CTC
Cuts at: 1055 1055
Size: 4043
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 820 1039 1868 2300 3047 3315 581
Size: 239 219 829 432 747 268 1309
Fragments arranged by size:
1309 829 747 432 268 239 219
EcoNI CCTnn'n_nnAGG
Cuts at: 483 483
Size: 4043
EcoO109I rG'GnC_Cy
Cuts at: 1182 1182
Size: 4043
EcoRI G'AATT_C
Cuts at: 1047 1047
Size: 4043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 4043
FspI TGC'GCA
Cuts at: 1824 1824
Size: 4043
HaeII r_GCGC'y
Cuts at: 828 1477 1725 2747 3726 828
Size: 649 248 1022 979 1145
Fragments arranged by size:
1145 1022 979 649 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 554 586 1885 1915 1917 1947 554
Size: 32 1299 30 2 30 2650
Fragments arranged by size:
2650 1299 32 30 30 2
HincII GTy'rAC
Cuts at: 1082 1082
Size: 4043
KasI G'GCGC_C
Cuts at: 824 1473 1721 824
Size: 649 248 3146
Fragments arranged by size:
3146 649 248
KpnI G_GTAC'C
Cuts at: 492 1063 492
Size: 571 3472
MluI A'CGCG_T
Cuts at: 3476 3476
Size: 4043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2714 2898 2714
Size: 184 3859
MscI TGG'CCA
Cuts at: 1804 1804
Size: 4043
MslI CAynn'nnrTG
Cuts at: 472 2032 2159 472
Size: 1560 127 2356
Fragments arranged by size:
2356 1560 127
MspA1I CmG'CkG
Cuts at: 237 814 937 1154 1468 1828 2841 3086 237
Size: 577 123 217 314 360 1013 245 1194
Fragments arranged by size:
1194 1013 577 360 314 245 217 123
NaeI GCC'GGC
Cuts at: 576 2224 576
Size: 1648 2395
NarI GG'CG_CC
Cuts at: 825 1474 1722 825
Size: 649 248 3146
Fragments arranged by size:
3146 649 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4043
NgoMIV G'CCGG_C
Cuts at: 574 2222 574
Size: 1648 2395
NsiI A_TGCA'T
Cuts at: 587 587
Size: 4043
NspI r_CATG'y
Cuts at: 336 1096 1596 2503 336
Size: 760 500 907 1876
Fragments arranged by size:
1876 907 760 500
PciI A'CATG_T
Cuts at: 332 2499 332
Size: 2167 1876
PfoI T'CCnGG_A
Cuts at: 326 326
Size: 4043
PmeI GTTT'AAAC
Cuts at: 1042 1042
Size: 4043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3182 3214 3182
Size: 32 4011
PspOMI G'GGCC_C
Cuts at: 631 631
Size: 4043
PstI C_TGCA'G
Cuts at: 1090 1775 1090
Size: 685 3358
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4043
PvuII CAG'CTG
Cuts at: 237 1468 1828 237
Size: 1231 360 2452
Fragments arranged by size:
2452 1231 360
RsrII CG'GwC_CG
Cuts at: 2238 2238
Size: 4043
SacI G_AGCT'C
Cuts at: 1057 1057
Size: 4043
SalI G'TCGA_C
Cuts at: 1080 1080
Size: 4043
SapI GCTCTTCn'nnn_
Cuts at: 2066 2276 2066
Size: 210 3833
SbfI CC_TGCA'GG
Cuts at: 1090 1090
Size: 4043
ScaI AGT'ACT
Cuts at: 3560 3560
Size: 4043
SfcI C'TryA_G
Cuts at: 33 1086 1771 2764 2955 33
Size: 1053 685 993 191 1121
Fragments arranged by size:
1121 1053 993 685 191
SfoI GGC'GCC
Cuts at: 826 1475 1723 826
Size: 649 248 3146
Fragments arranged by size:
3146 649 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4043
SmaI CCC'GGG
Cuts at: 1065 1065
Size: 4043
SmlI C'TyrA_G
Cuts at: 1198 2113 2605 2867 1198
Size: 915 492 262 2374
Fragments arranged by size:
2374 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4043
SphI G_CATG'C
Cuts at: 1096 1596 1096
Size: 500 3543
StyI C'CwwG_G
Cuts at: 107 1177 1531 107
Size: 1070 354 2619
Fragments arranged by size:
2619 1070 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 466 1626 1759 2218 2427 466
Size: 1160 133 459 209 2082
Fragments arranged by size:
2082 1160 459 209 133
TatI w'GTAC_w
Cuts at: 299 3558 299
Size: 3259 784
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3560 3592 3560
Size: 32 4011
Tth111I GACn'n_nGTC
Cuts at: 1840 1840
Size: 4043
XbaI T'CTAG_A
Cuts at: 1074 1074
Size: 4043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 921 1538 921
Size: 617 3426
XmaI C'CCGG_G
Cuts at: 1063 1063
Size: 4043
ZraI GAC'GTC
Cuts at: 729 729
Size: 4043
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BssHII BstEII BstZ17I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI