Restriction map for
pF1KE0851
(Circular) (Six-base) MAPSORT of: pF1KE0851.seq Check: 2869 from: 1 to: 4016
pF1KE0851 4016 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 808 808
Size: 4016
AccI GT'mk_AC
Cuts at: 1054 1054
Size: 4016
Acc65I G'GTAC_C
Cuts at: 1032 1032
Size: 4016
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1841 2273 3020 3288 1841
Size: 432 747 268 2569
Fragments arranged by size:
2569 747 432 268
AflIII A'CryG_T
Cuts at: 1368 2472 3449 1368
Size: 1104 977 1935
Fragments arranged by size:
1935 1104 977
AgeI A'CCGG_T
Cuts at: 2404 2404
Size: 4016
AleI CACnn'nnGTG
Cuts at: 381 381
Size: 4016
AlwNI CAG_nnn'CTG
Cuts at: 1321 2888 1321
Size: 1567 2449
ApaLI G'TGCA_C
Cuts at: 2786 2786
Size: 4016
ApoI r'AATT_y
Cuts at: 112 230 566 1020 3369 3625 112
Size: 118 336 454 2349 256 503
Fragments arranged by size:
2349 503 454 336 256 118
AseI AT'TA_AT
Cuts at: 20 602 20
Size: 582 3434
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 1036 1036
Size: 4016
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 705 1032 1446 1694 1729 705
Size: 327 414 248 35 2992
Fragments arranged by size:
2992 414 327 248 35
BanII G_rGCy'C
Cuts at: 1030 2060 1030
Size: 1030 2986
BbeI G_GCGC'C
Cuts at: 1450 1698 1450
Size: 248 3768
BciVI GTATCCnnnnn_n'
Cuts at: 485 1914 2675 4016 485
Size: 1429 761 1341 485
Fragments arranged by size:
1429 1341 761 485
BclI T'GATC_A
Cuts at: 1536 1536
Size: 4016
BglII A'GATC_T
Cuts at: 1531 3568 1531
Size: 2037 1979
BlpI GC'TnA_GC
Cuts at: 1128 1128
Size: 4016
Bme1580I G_kGCm'C
Cuts at: 1641 1734 2790 1641
Size: 93 1056 2867
Fragments arranged by size:
2867 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1228 1309 1644 3788 1228
Size: 81 335 2144 1456
Fragments arranged by size:
2144 1456 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 229
Size: 4016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 619 1192 2071 2563 2861 619
Size: 573 879 492 298 1774
Fragments arranged by size:
1774 879 573 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3720 3720
Size: 4016
BsaAI yAC'GTr
Cuts at: 450 1369 450
Size: 919 3097
BsaBI GATnn'nnATC
Cuts at: 6 1530 3567 6
Size: 1524 2037 455
Fragments arranged by size:
2037 1524 455
BsaHI Gr'CG_yC
Cuts at: 1447 1695 2397 3893 1447
Size: 248 702 1496 1570
Fragments arranged by size:
1570 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1205 1726 2404 2678 2825 3236 1205
Size: 521 678 274 147 411 1985
Fragments arranged by size:
1985 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1441 2199 2776 3272 1441
Size: 758 577 496 2185
Fragments arranged by size:
2185 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1558 1604 2812 104
Size: 1454 46 1208 1308
Fragments arranged by size:
1454 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1030 1808 1998 2790 1030
Size: 778 190 792 2256
Fragments arranged by size:
2256 792 778 190
BsmI GAATG_Cn'
Cuts at: 326 503 326
Size: 177 3839
Bsp1286I G_dGCh'C
Cuts at: 1030 1641 1734 1808 1998 2060 2790 1030
Size: 611 93 74 190 62 730 2256
Fragments arranged by size:
2256 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3236 3236
Size: 4016
BspHI T'CATG_A
Cuts at: 3192 3192
Size: 4016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 808 832 1066 1280 1582 1963 808
Size: 24 234 214 302 381 2861
Fragments arranged by size:
2861 381 302 234 214 24
BsrBI CCG'CTC
Cuts at: 50 241 2308 2362 3846 4011 50
Size: 191 2067 54 1484 165 55
Fragments arranged by size:
2067 1484 191 165 55 54
BsrDI GCAATG_nn'
Cuts at: 210 1928 210
Size: 1718 2298
BsrFI r'CCGG_y
Cuts at: 2014 2195 2404 2014
Size: 181 209 3626
Fragments arranged by size:
3626 209 181
BssSI C'ACGA_G
Cuts at: 2287 2645 2287
Size: 358 3658
BstAPI GCAn_nnn'nTGC
Cuts at: 1393 1393
Size: 4016
BstBI TT'CG_AA
Cuts at: 2377 2377
Size: 4016
BstYI r'GATC_y
Cuts at: 1 1041 1508 1531 1866 2112 3124 3210 3222
Size: 1040 467 23 335 246 1012 86 12
Cuts at: 3222 3568 1
Size: 346 449
Fragments arranged by size:
1040 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 955 955
Size: 4016
BtgI C'CryG_G
Cuts at: 107 107
Size: 4016
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1966 2147 2310 1966
Size: 181 163 3672
Fragments arranged by size:
3672 181 163
BtsI GCAGTG_nn'
Cuts at: 1259 1259
Size: 4016
DraI TTT'AAA
Cuts at: 1015 1015
Size: 4016
DraIII CAC_nnn'GTG
Cuts at: 413 413
Size: 4016
DrdI GACnn_nn'nnGTC
Cuts at: 1722 2580 1722
Size: 858 3158
EaeI y'GGCC_r
Cuts at: 1601 1775 2166 2193 3538 1601
Size: 174 391 27 1345 2079
Fragments arranged by size:
2079 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1601 1601
Size: 4016
EarI CTCTTCn'nnn_
Cuts at: 2039 2249 2039
Size: 210 3806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2224 2534 2680 3826 2224
Size: 310 146 1146 2414
Fragments arranged by size:
2414 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1028 1028
Size: 4016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 1841 2273 3020 3288 229
Size: 1612 432 747 268 957
Fragments arranged by size:
1612 957 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1155 1155
Size: 4016
EcoRI G'AATT_C
Cuts at: 1020 1020
Size: 4016
EcoRV GAT'ATC
Cuts at: 969 969
Size: 4016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 4016
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 1450 1698 2720 3699 1450
Size: 248 1022 979 1767
Fragments arranged by size:
1767 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1858 1888 1890 1920 1858
Size: 30 2 30 3954
Fragments arranged by size:
3954 30 30 2
HincII GTy'rAC
Cuts at: 1055 1055
Size: 4016
HindIII A'AGCT_T
Cuts at: 637 637
Size: 4016
KasI G'GCGC_C
Cuts at: 1446 1694 1446
Size: 248 3768
KpnI G_GTAC'C
Cuts at: 1036 1036
Size: 4016
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 773 952 2687 2871 773
Size: 179 1735 184 1918
Fragments arranged by size:
1918 1735 184 179
MscI TGG'CCA
Cuts at: 1777 1777
Size: 4016
MslI CAynn'nnrTG
Cuts at: 381 658 2005 2132 381
Size: 277 1347 127 2265
Fragments arranged by size:
2265 1347 277 127
MspA1I CmG'CkG
Cuts at: 510 1127 1441 1801 2814 3059 510
Size: 617 314 360 1013 245 1467
Fragments arranged by size:
1467 1013 617 360 314 245
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 1447 1695 1447
Size: 248 3768
NcoI C'CATG_G
Cuts at: 107 107
Size: 4016
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NspI r_CATG'y
Cuts at: 563 1069 1569 2476 563
Size: 506 500 907 2103
Fragments arranged by size:
2103 907 506 500
PciI A'CATG_T
Cuts at: 2472 2472
Size: 4016
PfoI T'CCnGG_A
Cuts at: 523 523
Size: 4016
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3984
PstI C_TGCA'G
Cuts at: 1063 1748 1063
Size: 685 3331
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 510 1441 1801 510
Size: 931 360 2725
Fragments arranged by size:
2725 931 360
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 1030 1030
Size: 4016
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 2039 2249 2039
Size: 210 3806
SbfI CC_TGCA'GG
Cuts at: 1063 1063
Size: 4016
ScaI AGT'ACT
Cuts at: 3533 3533
Size: 4016
SfcI C'TryA_G
Cuts at: 33 542 1059 1744 2737 2928 33
Size: 509 517 685 993 191 1121
Fragments arranged by size:
1121 993 685 517 509 191
SfoI GGC'GCC
Cuts at: 1448 1696 1448
Size: 248 3768
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 1038 1038
Size: 4016
SmlI C'TyrA_G
Cuts at: 634 1171 2086 2578 2840 634
Size: 537 915 492 262 1810
Fragments arranged by size:
1810 915 537 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 1069 1569 1069
Size: 500 3516
SspI AAT'ATT
Cuts at: 354 354
Size: 4016
StyI C'CwwG_G
Cuts at: 107 1150 1504 107
Size: 1043 354 2619
Fragments arranged by size:
2619 1043 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1599 1732 2191 2400 1599
Size: 133 459 209 3215
Fragments arranged by size:
3215 459 209 133
TatI w'GTAC_w
Cuts at: 3531 3531
Size: 4016
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 3984
Tth111I GACn'n_nGTC
Cuts at: 1813 1813
Size: 4016
XbaI T'CTAG_A
Cuts at: 1047 1047
Size: 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1511 1511
Size: 4016
XmaI C'CCGG_G
Cuts at: 1036 1036
Size: 4016
XmnI GAAnn'nnTTC
Cuts at: 116 354 116
Size: 238 3778
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI EcoNI FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI ZraI