Restriction map for
pF1KE0849
(Circular) (Six-base) MAPSORT of: pF1KE0849.seq Check: 9361 from: 1 to: 4061
pF1KE0849 4061 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1099 1099
Size: 4061
Acc65I G'GTAC_C
Cuts at: 400 1077 400
Size: 677 3384
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1886 2318 3065 3333 1886
Size: 432 747 268 2614
Fragments arranged by size:
2614 747 432 268
AflIII A'CryG_T
Cuts at: 1413 2517 3494 1413
Size: 1104 977 1980
Fragments arranged by size:
1980 1104 977
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 722 722
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 1366 2933 1366
Size: 1567 2494
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 139 1065 3414 3670 139
Size: 926 2349 256 530
Fragments arranged by size:
2349 926 530 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 169 1081 169
Size: 912 3149
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 339 400 1077 1491 1739 1774 339
Size: 61 677 414 248 35 2626
Fragments arranged by size:
2626 677 414 248 61 35
BanII G_rGCy'C
Cuts at: 1075 2105 1075
Size: 1030 3031
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BciVI GTATCCnnnnn_n'
Cuts at: 672 1959 2720 4061 672
Size: 1287 761 1341 672
Fragments arranged by size:
1341 1287 761 672
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BglII A'GATC_T
Cuts at: 357 1576 3613 357
Size: 1219 2037 805
Fragments arranged by size:
2037 1219 805
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 1686 1779 2835 1686
Size: 93 1056 2912
Fragments arranged by size:
2912 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 649 1273 1354 1689 3833 649
Size: 624 81 335 2144 877
Fragments arranged by size:
2144 877 624 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2608 2906 1237
Size: 879 492 298 2392
Fragments arranged by size:
2392 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 1414 1414
Size: 4061
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 1492 1740 2442 3938 1492
Size: 248 702 1496 1615
Fragments arranged by size:
1615 1496 702 248
BsaWI w'CCGG_w
Cuts at: 559 1250 1771 2449 2723 2870 3281 559
Size: 691 521 678 274 147 411 1339
Fragments arranged by size:
1339 691 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1486 2244 2821 3317 1486
Size: 758 577 496 2230
Fragments arranged by size:
2230 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1075 1853 2043 2835 1075
Size: 778 190 792 2301
Fragments arranged by size:
2301 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1075 1686 1779 1853 2043 2105 2835 1075
Size: 611 93 74 190 62 730 2301
Fragments arranged by size:
2301 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 893 1111 1325 1627 2008 893
Size: 218 214 302 381 2946
Fragments arranged by size:
2946 381 302 218 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1973 1973
Size: 4061
BsrFI r'CCGG_y
Cuts at: 2059 2240 2449 2059
Size: 181 209 3671
Fragments arranged by size:
3671 209 181
BssSI C'ACGA_G
Cuts at: 2332 2690 2332
Size: 358 3703
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstYI r'GATC_y
Cuts at: 1 261 357 1086 1553 1576 1911 2157 3169
Size: 260 96 729 467 23 335 246 1012
Cuts at: 3169 3255 3267 3613 1
Size: 86 12 346 449
Fragments arranged by size:
1012 729 467 449 346 335 260 246
96 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 982 982
Size: 4061
BtgI C'CryG_G
Cuts at: 626 626
Size: 4061
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 1304 1304
Size: 4061
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 608 643 608
Size: 35 4026
DraI TTT'AAA
Cuts at: 1060 1060
Size: 4061
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 203 479 539 804 1646 1820 2211 2238 3583
Size: 276 60 265 842 174 391 27 1345
Cuts at: 3583 203
Size: 681
Fragments arranged by size:
1345 842 681 391 276 265 174 60
27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 862 2084 2294 862
Size: 1222 210 2629
Fragments arranged by size:
2629 1222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1886 2318 3065 3333 1886
Size: 432 747 268 2614
Fragments arranged by size:
2614 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 521 1200 521
Size: 679 3382
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 4061
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 1495 1743 2765 3744 1495
Size: 248 1022 979 1812
Fragments arranged by size:
1812 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 349 381 1008 1040 1903 1933 1935 1965 349
Size: 32 627 32 863 30 2 30 2445
Fragments arranged by size:
2445 863 627 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 404 1081 404
Size: 677 3384
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2732 2916 2732
Size: 184 3877
MscI TGG'CCA
Cuts at: 205 481 541 1822 205
Size: 276 60 1281 2444
Fragments arranged by size:
2444 1281 276 60
MslI CAynn'nnrTG
Cuts at: 847 955 2050 2177 847
Size: 108 1095 127 2731
Fragments arranged by size:
2731 1095 127 108
MspA1I CmG'CkG
Cuts at: 1172 1486 1846 2859 3104 1172
Size: 314 360 1013 245 2129
Fragments arranged by size:
2129 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NspI r_CATG'y
Cuts at: 1114 1614 2521 1114
Size: 500 907 2654
Fragments arranged by size:
2654 907 500
PciI A'CATG_T
Cuts at: 2517 2517
Size: 4061
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 4029
PpuMI rG'GwC_Cy
Cuts at: 521 521
Size: 4061
PstI C_TGCA'G
Cuts at: 1108 1793 1108
Size: 685 3376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 1486 1846 1486
Size: 360 3701
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 2084 2294 2084
Size: 210 3851
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 580 1104 1789 2782 2973 33
Size: 547 524 685 993 191 1121
Fragments arranged by size:
1121 993 685 547 524 191
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 1216 2131 2623 2885 1216
Size: 915 492 262 2392
Fragments arranged by size:
2392 915 492 262
SpeI A'CTAG_T
Cuts at: 67 666 67
Size: 599 3462
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 329 370 1195 1549 329
Size: 41 825 354 2841
Fragments arranged by size:
2841 825 354 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 294 1644 1777 2236 2445 294
Size: 1350 133 459 209 1910
Fragments arranged by size:
1910 1350 459 209 133
TatI w'GTAC_w
Cuts at: 284 410 3576 284
Size: 126 3166 769
Fragments arranged by size:
3166 769 126
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 546 578 3578 3610 546
Size: 32 3000 32 997
Fragments arranged by size:
3000 997 32 32
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1556 1556
Size: 4061
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
XmnI GAAnn'nnTTC
Cuts at: 143 143
Size: 4061
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI