Restriction map for
pF1KE0844
(Circular) (Six-base) MAPSORT of: pF1KE0844.seq Check: 9645 from: 1 to: 4016
pF1KE0844 4016 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 409 409
Size: 4016
AccI GT'mk_AC
Cuts at: 983 1054 983
Size: 71 3945
Acc65I G'GTAC_C
Cuts at: 1032 1032
Size: 4016
AclI AA'CG_TT
Cuts at: 3856 3856
Size: 4016
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 899 1841 2273 3020 3288 899
Size: 942 432 747 268 1627
Fragments arranged by size:
1627 942 747 432 268
AflIII A'CryG_T
Cuts at: 1368 2472 3449 1368
Size: 1104 977 1935
Fragments arranged by size:
1935 1104 977
AgeI A'CCGG_T
Cuts at: 2404 2404
Size: 4016
AleI CACnn'nnGTG
Cuts at: 832 832
Size: 4016
AlwNI CAG_nnn'CTG
Cuts at: 642 1321 2888 642
Size: 679 1567 1770
Fragments arranged by size:
1770 1567 679
ApaLI G'TGCA_C
Cuts at: 2786 2786
Size: 4016
ApoI r'AATT_y
Cuts at: 1020 3369 3625 1020
Size: 2349 256 1411
Fragments arranged by size:
2349 1411 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4016
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
AvaI C'yCGr_G
Cuts at: 1036 1036
Size: 4016
BamHI G'GATC_C
Cuts at: 1041 1041
Size: 4016
BanI G'GyrC_C
Cuts at: 394 1032 1446 1694 1729 394
Size: 638 414 248 35 2681
Fragments arranged by size:
2681 638 414 248 35
BanII G_rGCy'C
Cuts at: 689 1030 2060 689
Size: 341 1030 2645
Fragments arranged by size:
2645 1030 341
BbeI G_GCGC'C
Cuts at: 1450 1698 1450
Size: 248 3768
BbsI GAAGACnn'nnnn_
Cuts at: 408 830 408
Size: 422 3594
BciVI GTATCCnnnnn_n'
Cuts at: 595 1914 2675 4016 595
Size: 1319 761 1341 595
Fragments arranged by size:
1341 1319 761 595
BclI T'GATC_A
Cuts at: 425 1536 425
Size: 1111 2905
BglII A'GATC_T
Cuts at: 1531 3568 1531
Size: 2037 1979
BlpI GC'TnA_GC
Cuts at: 1128 1128
Size: 4016
Bme1580I G_kGCm'C
Cuts at: 1641 1734 2790 1641
Size: 93 1056 2867
Fragments arranged by size:
2867 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 221 971 1228 1309 1644 3788 221
Size: 750 257 81 335 2144 449
Fragments arranged by size:
2144 750 449 335 257 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4016
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 423
Size: 4016
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 429 1192 2071 2563 2861 429
Size: 763 879 492 298 1584
Fragments arranged by size:
1584 879 763 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3720 3720
Size: 4016
BsaAI yAC'GTr
Cuts at: 1369 1369
Size: 4016
BsaBI GATnn'nnATC
Cuts at: 6 1530 3567 6
Size: 1524 2037 455
Fragments arranged by size:
2037 1524 455
BsaHI Gr'CG_yC
Cuts at: 1447 1695 2397 3893 1447
Size: 248 702 1496 1570
Fragments arranged by size:
1570 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1205 1726 2404 2678 2825 3236 1205
Size: 521 678 274 147 411 1985
Fragments arranged by size:
1985 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 835 865 835
Size: 30 3986
BseYI C'CCAG_C
Cuts at: 1441 2199 2776 3272 1441
Size: 758 577 496 2185
Fragments arranged by size:
2185 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 237 709 192
Size: 45 472 3499
Fragments arranged by size:
3499 472 45
BsiEI CG_ry'CG
Cuts at: 104 1558 1604 2812 104
Size: 1454 46 1208 1308
Fragments arranged by size:
1454 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 829 1030 1808 1998 2790 829
Size: 201 778 190 792 2055
Fragments arranged by size:
2055 792 778 201 190
Bsp1286I G_dGCh'C
Cuts at: 689 829 1030 1641 1734 1808 1998 2060 2790
Size: 140 201 611 93 74 190 62 730
Cuts at: 2790 689
Size: 1915
Fragments arranged by size:
1915 730 611 201 190 140 93 74
62
BspEI T'CCGG_A
Cuts at: 3236 3236
Size: 4016
BspHI T'CATG_A
Cuts at: 962 3192 962
Size: 2230 1786
BspMI ACCTGCnnnn'nnnn_
Cuts at: 209 409 1066 1280 1582 1963 209
Size: 200 657 214 302 381 2262
Fragments arranged by size:
2262 657 381 302 214 200
BsrBI CCG'CTC
Cuts at: 50 2308 2362 3846 4011 50
Size: 2258 54 1484 165 55
Fragments arranged by size:
2258 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 656 1928 656
Size: 1272 2744
BsrFI r'CCGG_y
Cuts at: 2014 2195 2404 2014
Size: 181 209 3626
Fragments arranged by size:
3626 209 181
BssSI C'ACGA_G
Cuts at: 2287 2645 2287
Size: 358 3658
BstAPI GCAn_nnn'nTGC
Cuts at: 215 401 1393 215
Size: 186 992 2838
Fragments arranged by size:
2838 992 186
BstBI TT'CG_AA
Cuts at: 2377 2377
Size: 4016
BstYI r'GATC_y
Cuts at: 1 1041 1508 1531 1866 2112 3124 3210 3222
Size: 1040 467 23 335 246 1012 86 12
Cuts at: 3222 3568 1
Size: 346 449
Fragments arranged by size:
1040 1012 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 984 984
Size: 4016
BtgI C'CryG_G
Cuts at: 107 152 710 107
Size: 45 558 3413
Fragments arranged by size:
3413 558 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1966 2147 2310 1966
Size: 181 163 3672
Fragments arranged by size:
3672 181 163
BtsI GCAGTG_nn'
Cuts at: 134 640 1259 134
Size: 506 619 2891
Fragments arranged by size:
2891 619 506
DraI TTT'AAA
Cuts at: 1015 1015
Size: 4016
DraIII CAC_nnn'GTG
Cuts at: 215 646 215
Size: 431 3585
DrdI GACnn_nn'nnGTC
Cuts at: 1722 2580 1722
Size: 858 3158
EaeI y'GGCC_r
Cuts at: 1601 1775 2166 2193 3538 1601
Size: 174 391 27 1345 2079
Fragments arranged by size:
2079 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1601 1601
Size: 4016
EarI CTCTTCn'nnn_
Cuts at: 2039 2249 2039
Size: 210 3806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2224 2534 2680 3826 2224
Size: 310 146 1146 2414
Fragments arranged by size:
2414 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1028 1028
Size: 4016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 899 1841 2273 3020 3288 423
Size: 476 942 432 747 268 1151
Fragments arranged by size:
1151 942 747 476 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1155 1155
Size: 4016
EcoRI G'AATT_C
Cuts at: 1020 1020
Size: 4016
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2035 2035
Size: 4016
FspI TGC'GCA
Cuts at: 1797 1797
Size: 4016
HaeII r_GCGC'y
Cuts at: 1450 1698 2720 3699 1450
Size: 248 1022 979 1767
Fragments arranged by size:
1767 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 314 346 1858 1888 1890 1920 314
Size: 32 1512 30 2 30 2410
Fragments arranged by size:
2410 1512 32 30 30 2
HincII GTy'rAC
Cuts at: 1055 1055
Size: 4016
KasI G'GCGC_C
Cuts at: 1446 1694 1446
Size: 248 3768
KpnI G_GTAC'C
Cuts at: 1036 1036
Size: 4016
MluI A'CGCG_T
Cuts at: 3449 3449
Size: 4016
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2687 2871 2687
Size: 184 3832
MscI TGG'CCA
Cuts at: 1777 1777
Size: 4016
MslI CAynn'nnrTG
Cuts at: 550 583 820 832 2005 2132 550
Size: 33 237 12 1173 127 2434
Fragments arranged by size:
2434 1173 237 127 33 12
MspA1I CmG'CkG
Cuts at: 281 741 1127 1441 1801 2814 3059 281
Size: 460 386 314 360 1013 245 1238
Fragments arranged by size:
1238 1013 460 386 360 314 245
NaeI GCC'GGC
Cuts at: 2197 2197
Size: 4016
NarI GG'CG_CC
Cuts at: 1447 1695 1447
Size: 248 3768
NcoI C'CATG_G
Cuts at: 107 710 107
Size: 603 3413
NgoMIV G'CCGG_C
Cuts at: 2195 2195
Size: 4016
NspI r_CATG'y
Cuts at: 487 525 1069 1569 2476 487
Size: 38 544 500 907 2027
Fragments arranged by size:
2027 907 544 500 38
PciI A'CATG_T
Cuts at: 2472 2472
Size: 4016
PflMI CCAn_nnn'nTGG
Cuts at: 303 372 303
Size: 69 3947
PfoI T'CCnGG_A
Cuts at: 439 439
Size: 4016
PmeI GTTT'AAAC
Cuts at: 1015 1015
Size: 4016
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3155 3187 3155
Size: 32 3984
PstI C_TGCA'G
Cuts at: 1063 1748 1063
Size: 685 3331
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4016
PvuII CAG'CTG
Cuts at: 1441 1801 1441
Size: 360 3656
RsrII CG'GwC_CG
Cuts at: 2211 2211
Size: 4016
SacI G_AGCT'C
Cuts at: 1030 1030
Size: 4016
SalI G'TCGA_C
Cuts at: 1053 1053
Size: 4016
SapI GCTCTTCn'nnn_
Cuts at: 2039 2249 2039
Size: 210 3806
SbfI CC_TGCA'GG
Cuts at: 1063 1063
Size: 4016
ScaI AGT'ACT
Cuts at: 3533 3533
Size: 4016
SfcI C'TryA_G
Cuts at: 33 1059 1744 2737 2928 33
Size: 1026 685 993 191 1121
Fragments arranged by size:
1121 1026 993 685 191
SfoI GGC'GCC
Cuts at: 1448 1696 1448
Size: 248 3768
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4016
SmaI CCC'GGG
Cuts at: 1038 1038
Size: 4016
SmlI C'TyrA_G
Cuts at: 408 1171 2086 2578 2840 408
Size: 763 915 492 262 1584
Fragments arranged by size:
1584 915 763 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4016
SphI G_CATG'C
Cuts at: 1069 1569 1069
Size: 500 3516
StyI C'CwwG_G
Cuts at: 107 710 1150 1504 107
Size: 603 440 354 2619
Fragments arranged by size:
2619 603 440 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 203 332 1599 1732 2191 2400 203
Size: 129 1267 133 459 209 1819
Fragments arranged by size:
1819 1267 459 209 133 129
TatI w'GTAC_w
Cuts at: 3531 3531
Size: 4016
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 3984
Tth111I GACn'n_nGTC
Cuts at: 1813 1813
Size: 4016
XbaI T'CTAG_A
Cuts at: 1047 1047
Size: 4016
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 900 1511 900
Size: 611 3405
XmaI C'CCGG_G
Cuts at: 1036 1036
Size: 4016
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
Bpu10I BseRI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI