Restriction map for
pF1KE0843
(Circular) (Six-base) MAPSORT of: pF1KE0843.seq Check: 2503 from: 1 to: 4172
pF1KE0843 4172 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 563 563
Size: 4172
AccI GT'mk_AC
Cuts at: 1210 1210
Size: 4172
Acc65I G'GTAC_C
Cuts at: 1188 1188
Size: 4172
AclI AA'CG_TT
Cuts at: 4012 4012
Size: 4172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 1997 2429 3176 3444 170
Size: 1827 432 747 268 898
Fragments arranged by size:
1827 898 747 432 268
AflIII A'CryG_T
Cuts at: 1524 2628 3605 1524
Size: 1104 977 2091
Fragments arranged by size:
2091 1104 977
AgeI A'CCGG_T
Cuts at: 2560 2560
Size: 4172
AlwNI CAG_nnn'CTG
Cuts at: 1477 3044 1477
Size: 1567 2605
ApaI G_GGCC'C
Cuts at: 639 639
Size: 4172
ApaLI G'TGCA_C
Cuts at: 2942 2942
Size: 4172
ApoI r'AATT_y
Cuts at: 294 1176 3525 3781 294
Size: 882 2349 256 685
Fragments arranged by size:
2349 882 685 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4172
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4172
AvaI C'yCGr_G
Cuts at: 1192 1192
Size: 4172
BamHI G'GATC_C
Cuts at: 284 1197 284
Size: 913 3259
BanI G'GyrC_C
Cuts at: 1188 1602 1850 1885 1188
Size: 414 248 35 3475
Fragments arranged by size:
3475 414 248 35
BanII G_rGCy'C
Cuts at: 639 949 1186 2216 639
Size: 310 237 1030 2595
Fragments arranged by size:
2595 1030 310 237
BbeI G_GCGC'C
Cuts at: 1606 1854 1606
Size: 248 3924
BbsI GAAGACnn'nnnn_
Cuts at: 828 828
Size: 4172
BbvCI CC'TCA_GC
Cuts at: 514 514
Size: 4172
BciVI GTATCCnnnnn_n'
Cuts at: 407 718 2070 2831 4172 407
Size: 311 1352 761 1341 407
Fragments arranged by size:
1352 1341 761 407 311
BclI T'GATC_A
Cuts at: 1692 1692
Size: 4172
BfrBI ATG'CAT
Cuts at: 658 658
Size: 4172
BglII A'GATC_T
Cuts at: 1687 3724 1687
Size: 2037 2135
BlpI GC'TnA_GC
Cuts at: 1284 1284
Size: 4172
Bme1580I G_kGCm'C
Cuts at: 502 639 1797 1890 2946 502
Size: 137 1158 93 1056 1728
Fragments arranged by size:
1728 1158 1056 137 93
BmrI ACTGGGnnnn_n'
Cuts at: 557 1384 1465 1800 3944 557
Size: 827 81 335 2144 785
Fragments arranged by size:
2144 827 785 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 529 1112 2539 529
Size: 583 1427 2162
Fragments arranged by size:
2162 1427 583
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 296
Size: 4172
Bpu10I CC'TnA_GC
Cuts at: 514 514
Size: 4172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1348 2227 2719 3017 1348
Size: 879 492 298 2503
Fragments arranged by size:
2503 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 342 3876 342
Size: 3534 638
BsaAI yAC'GTr
Cuts at: 1525 1525
Size: 4172
BsaBI GATnn'nnATC
Cuts at: 6 583 1686 3723 6
Size: 577 1103 2037 455
Fragments arranged by size:
2037 1103 577 455
BsaHI Gr'CG_yC
Cuts at: 1603 1851 2553 4049 1603
Size: 248 702 1496 1726
Fragments arranged by size:
1726 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1361 1882 2560 2834 2981 3392 1361
Size: 521 678 274 147 411 2141
Fragments arranged by size:
2141 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 874 904 874
Size: 30 4142
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 616 616
Size: 4172
BseYI C'CCAG_C
Cuts at: 632 1059 1597 2355 2932 3428 632
Size: 427 538 758 577 496 1376
Fragments arranged by size:
1376 758 577 538 496 427
BsiEI CG_ry'CG
Cuts at: 104 1714 1760 2968 104
Size: 1610 46 1208 1308
Fragments arranged by size:
1610 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 949 1186 1964 2154 2946 949
Size: 237 778 190 792 2175
Fragments arranged by size:
2175 792 778 237 190
BsmI GAATG_Cn'
Cuts at: 660 660
Size: 4172
Bsp1286I G_dGCh'C
Cuts at: 502 639 949 1186 1797 1890 1964 2154 2216
Size: 137 310 237 611 93 74 190 62
Cuts at: 2216 2946 502
Size: 730 1728
Fragments arranged by size:
1728 730 611 310 237 190 137 93
74 62
BspEI T'CCGG_A
Cuts at: 3392 3392
Size: 4172
BspHI T'CATG_A
Cuts at: 1085 3348 1085
Size: 2263 1909
BspMI ACCTGCnnnn'nnnn_
Cuts at: 563 1222 1436 1738 2119 563
Size: 659 214 302 381 2616
Fragments arranged by size:
2616 659 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2464 2518 4002 4167 50
Size: 2414 54 1484 165 55
Fragments arranged by size:
2414 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2084 2084
Size: 4172
BsrFI r'CCGG_y
Cuts at: 2170 2351 2560 2170
Size: 181 209 3782
Fragments arranged by size:
3782 209 181
BssSI C'ACGA_G
Cuts at: 2443 2801 2443
Size: 358 3814
BstAPI GCAn_nnn'nTGC
Cuts at: 524 1549 524
Size: 1025 3147
BstBI TT'CG_AA
Cuts at: 2533 2533
Size: 4172
BstXI CCAn_nnnn'nTGG
Cuts at: 1019 1019
Size: 4172
BstYI r'GATC_y
Cuts at: 1 284 595 1197 1664 1687 2022 2268 3280
Size: 283 311 602 467 23 335 246 1012
Cuts at: 3280 3366 3378 3724 1
Size: 86 12 346 449
Fragments arranged by size:
1012 602 467 449 346 335 311 283
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 353 392 830 107
Size: 246 39 438 3449
Fragments arranged by size:
3449 438 246 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2122 2303 2466 2122
Size: 181 163 3828
Fragments arranged by size:
3828 181 163
BtsI GCAGTG_nn'
Cuts at: 1415 1415
Size: 4172
DraI TTT'AAA
Cuts at: 408 1171 408
Size: 763 3409
DraIII CAC_nnn'GTG
Cuts at: 495 495
Size: 4172
DrdI GACnn_nn'nnGTC
Cuts at: 1878 2736 1878
Size: 858 3314
EaeI y'GGCC_r
Cuts at: 1757 1931 2322 2349 3694 1757
Size: 174 391 27 1345 2235
Fragments arranged by size:
2235 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1757 1757
Size: 4172
EarI CTCTTCn'nnn_
Cuts at: 2195 2405 2195
Size: 210 3962
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2380 2690 2836 3982 2380
Size: 310 146 1146 2570
Fragments arranged by size:
2570 1146 310 146
EcoICRI GAG'CTC
Cuts at: 947 1184 947
Size: 237 3935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 296 1997 2429 3176 3444 170
Size: 126 1701 432 747 268 898
Fragments arranged by size:
1701 898 747 432 268 126
EcoO109I rG'GnC_Cy
Cuts at: 636 1311 636
Size: 675 3497
EcoRI G'AATT_C
Cuts at: 1176 1176
Size: 4172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 495 2191 495
Size: 1696 2476
FspI TGC'GCA
Cuts at: 1953 1953
Size: 4172
HaeII r_GCGC'y
Cuts at: 1606 1854 2876 3855 1606
Size: 248 1022 979 1923
Fragments arranged by size:
1923 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 269 301 2014 2044 2046 2076 269
Size: 32 1713 30 2 30 2365
Fragments arranged by size:
2365 1713 32 30 30 2
HincII GTy'rAC
Cuts at: 1211 1211
Size: 4172
KasI G'GCGC_C
Cuts at: 1602 1850 1602
Size: 248 3924
KpnI G_GTAC'C
Cuts at: 1192 1192
Size: 4172
MluI A'CGCG_T
Cuts at: 3605 3605
Size: 4172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 93 891 2843 3027 93
Size: 798 1952 184 1238
Fragments arranged by size:
1952 1238 798 184
MscI TGG'CCA
Cuts at: 1933 1933
Size: 4172
MslI CAynn'nnrTG
Cuts at: 931 2161 2288 931
Size: 1230 127 2815
Fragments arranged by size:
2815 1230 127
MspA1I CmG'CkG
Cuts at: 1283 1597 1957 2970 3215 1283
Size: 314 360 1013 245 2240
Fragments arranged by size:
2240 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2353 2353
Size: 4172
NarI GG'CG_CC
Cuts at: 1603 1851 1603
Size: 248 3924
NcoI C'CATG_G
Cuts at: 107 392 830 107
Size: 285 438 3449
Fragments arranged by size:
3449 438 285
NgoMIV G'CCGG_C
Cuts at: 2351 2351
Size: 4172
NsiI A_TGCA'T
Cuts at: 660 660
Size: 4172
NspI r_CATG'y
Cuts at: 769 1225 1725 2632 769
Size: 456 500 907 2309
Fragments arranged by size:
2309 907 500 456
PciI A'CATG_T
Cuts at: 2628 2628
Size: 4172
PmeI GTTT'AAAC
Cuts at: 1171 1171
Size: 4172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 3311
Size: 32 4140
PsiI TTA'TAA
Cuts at: 317 317
Size: 4172
PspOMI G'GGCC_C
Cuts at: 635 635
Size: 4172
PstI C_TGCA'G
Cuts at: 898 1126 1219 1904 898
Size: 228 93 685 3166
Fragments arranged by size:
3166 685 228 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4172
PvuII CAG'CTG
Cuts at: 1597 1957 1597
Size: 360 3812
RsrII CG'GwC_CG
Cuts at: 2367 2367
Size: 4172
SacI G_AGCT'C
Cuts at: 949 1186 949
Size: 237 3935
SalI G'TCGA_C
Cuts at: 1209 1209
Size: 4172
SapI GCTCTTCn'nnn_
Cuts at: 2195 2405 2195
Size: 210 3962
SbfI CC_TGCA'GG
Cuts at: 1126 1219 1126
Size: 93 4079
ScaI AGT'ACT
Cuts at: 3689 3689
Size: 4172
SfcI C'TryA_G
Cuts at: 33 846 894 998 1122 1215 1900 2893 3084
Size: 813 48 104 124 93 685 993 191
Cuts at: 3084 33
Size: 1121
Fragments arranged by size:
1121 993 813 685 191 124 104 93
48
SfoI GGC'GCC
Cuts at: 1604 1852 1604
Size: 248 3924
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4172
SmaI CCC'GGG
Cuts at: 1194 1194
Size: 4172
SmlI C'TyrA_G
Cuts at: 1327 2242 2734 2996 1327
Size: 915 492 262 2503
Fragments arranged by size:
2503 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4172
SphI G_CATG'C
Cuts at: 1225 1725 1225
Size: 500 3672
SspI AAT'ATT
Cuts at: 448 448
Size: 4172
StyI C'CwwG_G
Cuts at: 107 380 392 601 830 1306 1660 107
Size: 273 12 209 229 476 354 2619
Fragments arranged by size:
2619 476 354 273 229 209 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1755 1888 2347 2556 1755
Size: 133 459 209 3371
Fragments arranged by size:
3371 459 209 133
TatI w'GTAC_w
Cuts at: 3687 3687
Size: 4172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3689 3721 3689
Size: 32 4140
Tth111I GACn'n_nGTC
Cuts at: 1969 1969
Size: 4172
XbaI T'CTAG_A
Cuts at: 1203 1203
Size: 4172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 828 936 1038 1667 828
Size: 108 102 629 3333
Fragments arranged by size:
3333 629 108 102
XmaI C'CCGG_G
Cuts at: 1192 1192
Size: 4172
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BglI BmgBI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI