Restriction map for
pF1KE0840
(Circular) (Six-base) MAPSORT of: pF1KE0840.seq Check: 5373 from: 1 to: 4148
pF1KE0840 4148 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 442 442
Size: 4148
AccI GT'mk_AC
Cuts at: 1186 1186
Size: 4148
Acc65I G'GTAC_C
Cuts at: 1164 1164
Size: 4148
AclI AA'CG_TT
Cuts at: 3988 3988
Size: 4148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 1973 2405 3152 3420 354
Size: 1619 432 747 268 1082
Fragments arranged by size:
1619 1082 747 432 268
AflIII A'CryG_T
Cuts at: 1500 2604 3581 1500
Size: 1104 977 2067
Fragments arranged by size:
2067 1104 977
AgeI A'CCGG_T
Cuts at: 2536 2536
Size: 4148
AlwNI CAG_nnn'CTG
Cuts at: 1453 3020 1453
Size: 1567 2581
ApaLI G'TGCA_C
Cuts at: 2918 2918
Size: 4148
ApoI r'AATT_y
Cuts at: 557 1122 1152 3501 3757 557
Size: 565 30 2349 256 948
Fragments arranged by size:
2349 948 565 256 30
AseI AT'TA_AT
Cuts at: 20 943 20
Size: 923 3225
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
AvaI C'yCGr_G
Cuts at: 200 1168 200
Size: 968 3180
BamHI G'GATC_C
Cuts at: 196 578 1173 196
Size: 382 595 3171
Fragments arranged by size:
3171 595 382
BanI G'GyrC_C
Cuts at: 1164 1578 1826 1861 1164
Size: 414 248 35 3451
Fragments arranged by size:
3451 414 248 35
BanII G_rGCy'C
Cuts at: 1162 2192 1162
Size: 1030 3118
BbeI G_GCGC'C
Cuts at: 1582 1830 1582
Size: 248 3900
BciVI GTATCCnnnnn_n'
Cuts at: 2046 2807 4148 2046
Size: 761 1341 2046
Fragments arranged by size:
2046 1341 761
BclI T'GATC_A
Cuts at: 1668 1668
Size: 4148
BglI GCCn_nnn'nGGC
Cuts at: 328 853 328
Size: 525 3623
BglII A'GATC_T
Cuts at: 1663 3700 1663
Size: 2037 2111
BlpI GC'TnA_GC
Cuts at: 1260 1260
Size: 4148
Bme1580I G_kGCm'C
Cuts at: 1773 1866 2922 1773
Size: 93 1056 2999
Fragments arranged by size:
2999 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 708 1360 1441 1776 3920 708
Size: 652 81 335 2144 936
Fragments arranged by size:
2144 936 652 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2515 2515
Size: 4148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 658
Size: 4148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1324 2203 2695 2993 1324
Size: 879 492 298 2479
Fragments arranged by size:
2479 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3852 3852
Size: 4148
BsaAI yAC'GTr
Cuts at: 1501 1501
Size: 4148
BsaBI GATnn'nnATC
Cuts at: 6 274 1662 3699 6
Size: 268 1388 2037 455
Fragments arranged by size:
2037 1388 455 268
BsaHI Gr'CG_yC
Cuts at: 1579 1827 2529 4025 1579
Size: 248 702 1496 1702
Fragments arranged by size:
1702 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1337 1858 2536 2810 2957 3368 1337
Size: 521 678 274 147 411 2117
Fragments arranged by size:
2117 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 405 435 405
Size: 30 4118
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 426 426
Size: 4148
BseYI C'CCAG_C
Cuts at: 1573 2331 2908 3404 1573
Size: 758 577 496 2317
Fragments arranged by size:
2317 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 280 160
Size: 120 4028
BsiEI CG_ry'CG
Cuts at: 104 1690 1736 2944 104
Size: 1586 46 1208 1308
Fragments arranged by size:
1586 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1162 1940 2130 2922 1162
Size: 778 190 792 2388
Fragments arranged by size:
2388 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1162 1773 1866 1940 2130 2192 2922 1162
Size: 611 93 74 190 62 730 2388
Fragments arranged by size:
2388 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3368 3368
Size: 4148
BspHI T'CATG_A
Cuts at: 3324 3324
Size: 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 442 1198 1412 1714 2095 442
Size: 756 214 302 381 2495
Fragments arranged by size:
2495 756 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2440 2494 3978 4143 50
Size: 2390 54 1484 165 55
Fragments arranged by size:
2390 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 500 2060 500
Size: 1560 2588
BsrFI r'CCGG_y
Cuts at: 2146 2327 2536 2146
Size: 181 209 3758
Fragments arranged by size:
3758 209 181
BsrGI T'GTAC_A
Cuts at: 212 593 212
Size: 381 3767
BssSI C'ACGA_G
Cuts at: 2419 2777 2419
Size: 358 3790
BstAPI GCAn_nnn'nTGC
Cuts at: 1525 1525
Size: 4148
BstBI TT'CG_AA
Cuts at: 2509 2509
Size: 4148
BstEII G'GTnAC_C
Cuts at: 920 920
Size: 4148
BstYI r'GATC_y
Cuts at: 1 196 578 1173 1640 1663 1998 2244 3256
Size: 195 382 595 467 23 335 246 1012
Cuts at: 3256 3342 3354 3700 1
Size: 86 12 346 449
Fragments arranged by size:
1012 595 467 449 382 346 335 246
195 86 23 12
BtgI C'CryG_G
Cuts at: 1054 1054
Size: 4148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2098 2279 2442 2098
Size: 181 163 3804
Fragments arranged by size:
3804 181 163
BtsI GCAGTG_nn'
Cuts at: 1391 1391
Size: 4148
DraI TTT'AAA
Cuts at: 1100 1147 1100
Size: 47 4101
DrdI GACnn_nn'nnGTC
Cuts at: 520 1854 2712 520
Size: 1334 858 1956
Fragments arranged by size:
1956 1334 858
EaeI y'GGCC_r
Cuts at: 854 1733 1907 2298 2325 3670 854
Size: 879 174 391 27 1345 1332
Fragments arranged by size:
1345 1332 879 391 174 27
EagI C'GGCC_G
Cuts at: 1733 1733
Size: 4148
EarI CTCTTCn'nnn_
Cuts at: 465 2171 2381 465
Size: 1706 210 2232
Fragments arranged by size:
2232 1706 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2356 2666 2812 3958 2356
Size: 310 146 1146 2546
Fragments arranged by size:
2546 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1160 1160
Size: 4148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 354 658 1973 2405 3152 3420 354
Size: 304 1315 432 747 268 1082
Fragments arranged by size:
1315 1082 747 432 304 268
EcoO109I rG'GnC_Cy
Cuts at: 167 1287 167
Size: 1120 3028
EcoRI G'AATT_C
Cuts at: 1152 1152
Size: 4148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2167 2167
Size: 4148
FspI TGC'GCA
Cuts at: 1929 1929
Size: 4148
HaeII r_GCGC'y
Cuts at: 1582 1830 2852 3831 1582
Size: 248 1022 979 1899
Fragments arranged by size:
1899 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 631 663 703 735 1990 2020 2022 2052 631
Size: 32 40 32 1255 30 2 30 2727
Fragments arranged by size:
2727 1255 40 32 32 30 30 2
HincII GTy'rAC
Cuts at: 707 1187 707
Size: 480 3668
KasI G'GCGC_C
Cuts at: 1578 1826 1578
Size: 248 3900
KpnI G_GTAC'C
Cuts at: 1168 1168
Size: 4148
MluI A'CGCG_T
Cuts at: 3581 3581
Size: 4148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 328 2819 3003 328
Size: 2491 184 1473
Fragments arranged by size:
2491 1473 184
MscI TGG'CCA
Cuts at: 856 1909 856
Size: 1053 3095
MslI CAynn'nnrTG
Cuts at: 568 2137 2264 568
Size: 1569 127 2452
Fragments arranged by size:
2452 1569 127
MspA1I CmG'CkG
Cuts at: 144 294 599 1259 1573 1933 2946 3191 144
Size: 150 305 660 314 360 1013 245 1101
Fragments arranged by size:
1101 1013 660 360 314 305 245 150
NaeI GCC'GGC
Cuts at: 2329 2329
Size: 4148
NarI GG'CG_CC
Cuts at: 1579 1827 1579
Size: 248 3900
NcoI C'CATG_G
Cuts at: 1054 1054
Size: 4148
NgoMIV G'CCGG_C
Cuts at: 2327 2327
Size: 4148
NspI r_CATG'y
Cuts at: 1201 1701 2608 1201
Size: 500 907 2741
Fragments arranged by size:
2741 907 500
PasI CC'CwG_GG
Cuts at: 885 885
Size: 4148
PciI A'CATG_T
Cuts at: 2604 2604
Size: 4148
PfoI T'CCnGG_A
Cuts at: 574 574
Size: 4148
PmeI GTTT'AAAC
Cuts at: 1147 1147
Size: 4148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 4116
PpuMI rG'GwC_Cy
Cuts at: 167 167
Size: 4148
PsiI TTA'TAA
Cuts at: 967 1013 967
Size: 46 4102
PstI C_TGCA'G
Cuts at: 1195 1880 1195
Size: 685 3463
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4148
PvuII CAG'CTG
Cuts at: 144 294 1573 1933 144
Size: 150 1279 360 2359
Fragments arranged by size:
2359 1279 360 150
RsrII CG'GwC_CG
Cuts at: 2343 2343
Size: 4148
SacI G_AGCT'C
Cuts at: 1162 1162
Size: 4148
SalI G'TCGA_C
Cuts at: 1185 1185
Size: 4148
SapI GCTCTTCn'nnn_
Cuts at: 2171 2381 2171
Size: 210 3938
SbfI CC_TGCA'GG
Cuts at: 1195 1195
Size: 4148
ScaI AGT'ACT
Cuts at: 3665 3665
Size: 4148
SfcI C'TryA_G
Cuts at: 33 1191 1876 2869 3060 33
Size: 1158 685 993 191 1121
Fragments arranged by size:
1158 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1580 1828 1580
Size: 248 3900
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4148
SmaI CCC'GGG
Cuts at: 202 1170 202
Size: 968 3180
SmlI C'TyrA_G
Cuts at: 1303 2218 2710 2972 1303
Size: 915 492 262 2479
Fragments arranged by size:
2479 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4148
SphI G_CATG'C
Cuts at: 1201 1701 1201
Size: 500 3648
StyI C'CwwG_G
Cuts at: 1054 1282 1636 1054
Size: 228 354 3566
Fragments arranged by size:
3566 354 228
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 340 690 1731 1864 2323 2532 340
Size: 350 1041 133 459 209 1956
Fragments arranged by size:
1956 1041 459 350 209 133
TatI w'GTAC_w
Cuts at: 212 421 593 3663 212
Size: 209 172 3070 697
Fragments arranged by size:
3070 697 209 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 188 220 3665 3697 188
Size: 32 3445 32 639
Fragments arranged by size:
3445 639 32 32
Tth111I GACn'n_nGTC
Cuts at: 1945 1945
Size: 4148
XbaI T'CTAG_A
Cuts at: 1179 1179
Size: 4148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1643 1643
Size: 4148
XmaI C'CCGG_G
Cuts at: 200 1168 200
Size: 968 3180
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI
BmtI Bpu10I BsiWI BsmI BsmBI BssHII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI