Restriction map for
pF1KE0838
(Circular) (Six-base) MAPSORT of: pF1KE0838.seq Check: 2821 from: 1 to: 4040
pF1KE0838 4040 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 418 418
Size: 4040
AccI GT'mk_AC
Cuts at: 1078 1078
Size: 4040
Acc65I G'GTAC_C
Cuts at: 1056 1056
Size: 4040
AclI AA'CG_TT
Cuts at: 3880 3880
Size: 4040
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 454 493 1865 2297 3044 3312 454
Size: 39 1372 432 747 268 1182
Fragments arranged by size:
1372 1182 747 432 268 39
AflIII A'CryG_T
Cuts at: 1009 1392 2496 3473 1009
Size: 383 1104 977 1576
Fragments arranged by size:
1576 1104 977 383
AgeI A'CCGG_T
Cuts at: 2428 2428
Size: 4040
AlwNI CAG_nnn'CTG
Cuts at: 1345 2912 1345
Size: 1567 2473
ApaLI G'TGCA_C
Cuts at: 496 2810 496
Size: 2314 1726
ApoI r'AATT_y
Cuts at: 154 272 1044 3393 3649 154
Size: 118 772 2349 256 545
Fragments arranged by size:
2349 772 545 256 118
AseI AT'TA_AT
Cuts at: 20 20
Size: 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
AvaI C'yCGr_G
Cuts at: 1060 1060
Size: 4040
BamHI G'GATC_C
Cuts at: 503 1065 503
Size: 562 3478
BanI G'GyrC_C
Cuts at: 1056 1470 1718 1753 1056
Size: 414 248 35 3343
Fragments arranged by size:
3343 414 248 35
BanII G_rGCy'C
Cuts at: 1054 2084 1054
Size: 1030 3010
BbeI G_GCGC'C
Cuts at: 1474 1722 1474
Size: 248 3792
BbsI GAAGACnn'nnnn_
Cuts at: 414 414
Size: 4040
BciVI GTATCCnnnnn_n'
Cuts at: 175 1938 2699 4040 175
Size: 1763 761 1341 175
Fragments arranged by size:
1763 1341 761 175
BclI T'GATC_A
Cuts at: 281 1560 281
Size: 1279 2761
BglII A'GATC_T
Cuts at: 724 1555 3592 724
Size: 831 2037 1172
Fragments arranged by size:
2037 1172 831
BlpI GC'TnA_GC
Cuts at: 1152 1152
Size: 4040
Bme1580I G_kGCm'C
Cuts at: 500 1665 1758 2814 500
Size: 1165 93 1056 1726
Fragments arranged by size:
1726 1165 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1252 1333 1668 3812 1252
Size: 81 335 2144 1480
Fragments arranged by size:
2144 1480 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 170
Size: 4040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1216 2095 2587 2885 1216
Size: 879 492 298 2371
Fragments arranged by size:
2371 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 770 3744 770
Size: 2974 1066
BsaAI yAC'GTr
Cuts at: 1393 1393
Size: 4040
BsaBI GATnn'nnATC
Cuts at: 6 1554 3591 6
Size: 1548 2037 455
Fragments arranged by size:
2037 1548 455
BsaHI Gr'CG_yC
Cuts at: 1471 1719 2421 3917 1471
Size: 248 702 1496 1594
Fragments arranged by size:
1594 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1229 1750 2428 2702 2849 3260 1229
Size: 521 678 274 147 411 2009
Fragments arranged by size:
2009 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 510 781 1465 2223 2800 3296 510
Size: 271 684 758 577 496 1254
Fragments arranged by size:
1254 758 684 577 496 271
BsiEI CG_ry'CG
Cuts at: 104 1582 1628 2836 104
Size: 1478 46 1208 1308
Fragments arranged by size:
1478 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 500 1054 1832 2022 2814 500
Size: 554 778 190 792 1726
Fragments arranged by size:
1726 792 778 554 190
BsmI GAATG_Cn'
Cuts at: 1007 1007
Size: 4040
Bsp1286I G_dGCh'C
Cuts at: 500 1054 1665 1758 1832 2022 2084 2814 500
Size: 554 611 93 74 190 62 730 1726
Fragments arranged by size:
1726 730 611 554 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3260 3260
Size: 4040
BspHI T'CATG_A
Cuts at: 3216 3216
Size: 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 418 1090 1304 1606 1987 418
Size: 672 214 302 381 2471
Fragments arranged by size:
2471 672 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2332 2386 3870 4035 50
Size: 2282 54 1484 165 55
Fragments arranged by size:
2282 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 185 1952 185
Size: 1767 2273
BsrFI r'CCGG_y
Cuts at: 766 2038 2219 2428 766
Size: 1272 181 209 2378
Fragments arranged by size:
2378 1272 209 181
BssSI C'ACGA_G
Cuts at: 2311 2669 2311
Size: 358 3682
BstAPI GCAn_nnn'nTGC
Cuts at: 1417 1417
Size: 4040
BstBI TT'CG_AA
Cuts at: 2401 2401
Size: 4040
BstYI r'GATC_y
Cuts at: 1 503 724 1065 1532 1555 1890 2136 3148
Size: 502 221 341 467 23 335 246 1012
Cuts at: 3148 3234 3246 3592 1
Size: 86 12 346 449
Fragments arranged by size:
1012 502 467 449 346 341 335 246
221 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1990 2171 2334 1990
Size: 181 163 3696
Fragments arranged by size:
3696 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1283
Size: 4040
DraI TTT'AAA
Cuts at: 971 1039 971
Size: 68 3972
DrdI GACnn_nn'nnGTC
Cuts at: 1746 2604 1746
Size: 858 3182
EaeI y'GGCC_r
Cuts at: 1625 1799 2190 2217 3562 1625
Size: 174 391 27 1345 2103
Fragments arranged by size:
2103 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1625 1625
Size: 4040
EarI CTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2248 2558 2704 3850 2248
Size: 310 146 1146 2438
Fragments arranged by size:
2438 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1052 1052
Size: 4040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 170 454 493 1865 2297 3044 3312 170
Size: 284 39 1372 432 747 268 898
Fragments arranged by size:
1372 898 747 432 284 268 39
EcoO109I rG'GnC_Cy
Cuts at: 1179 1179
Size: 4040
EcoRI G'AATT_C
Cuts at: 154 1044 154
Size: 890 3150
EcoRV GAT'ATC
Cuts at: 704 704
Size: 4040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 4040
FspI TGC'GCA
Cuts at: 1821 1821
Size: 4040
HaeII r_GCGC'y
Cuts at: 1474 1722 2744 3723 1474
Size: 248 1022 979 1791
Fragments arranged by size:
1791 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1882 1912 1914 1944 1882
Size: 30 2 30 3978
Fragments arranged by size:
3978 30 30 2
HincII GTy'rAC
Cuts at: 198 240 1079 198
Size: 42 839 3159
Fragments arranged by size:
3159 839 42
KasI G'GCGC_C
Cuts at: 1470 1718 1470
Size: 248 3792
KpnI G_GTAC'C
Cuts at: 1060 1060
Size: 4040
MfeI C'AATT_G
Cuts at: 587 677 587
Size: 90 3950
MluI A'CGCG_T
Cuts at: 3473 3473
Size: 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2895 2711
Size: 184 3856
MscI TGG'CCA
Cuts at: 1801 1801
Size: 4040
MslI CAynn'nnrTG
Cuts at: 2029 2156 2029
Size: 127 3913
MspA1I CmG'CkG
Cuts at: 1151 1465 1825 2838 3083 1151
Size: 314 360 1013 245 2108
Fragments arranged by size:
2108 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 4040
NarI GG'CG_CC
Cuts at: 1471 1719 1471
Size: 248 3792
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 4040
NspI r_CATG'y
Cuts at: 1013 1093 1593 2500 1013
Size: 80 500 907 2553
Fragments arranged by size:
2553 907 500 80
PciI A'CATG_T
Cuts at: 1009 2496 1009
Size: 1487 2553
PmeI GTTT'AAAC
Cuts at: 1039 1039
Size: 4040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3179 3211 3179
Size: 32 4008
PshAI GACnn'nnGTC
Cuts at: 994 994
Size: 4040
PsiI TTA'TAA
Cuts at: 136 136
Size: 4040
PstI C_TGCA'G
Cuts at: 123 1087 1772 123
Size: 964 685 2391
Fragments arranged by size:
2391 964 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4040
PvuII CAG'CTG
Cuts at: 1465 1825 1465
Size: 360 3680
RsrII CG'GwC_CG
Cuts at: 2235 2235
Size: 4040
SacI G_AGCT'C
Cuts at: 1054 1054
Size: 4040
SalI G'TCGA_C
Cuts at: 1077 1077
Size: 4040
SapI GCTCTTCn'nnn_
Cuts at: 2063 2273 2063
Size: 210 3830
SbfI CC_TGCA'GG
Cuts at: 1087 1087
Size: 4040
ScaI AGT'ACT
Cuts at: 317 560 3557 317
Size: 243 2997 800
Fragments arranged by size:
2997 800 243
SfcI C'TryA_G
Cuts at: 33 119 1083 1768 2761 2952 33
Size: 86 964 685 993 191 1121
Fragments arranged by size:
1121 993 964 685 191 86
SfoI GGC'GCC
Cuts at: 1472 1720 1472
Size: 248 3792
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4040
SmaI CCC'GGG
Cuts at: 1062 1062
Size: 4040
SmlI C'TyrA_G
Cuts at: 1195 2110 2602 2864 1195
Size: 915 492 262 2371
Fragments arranged by size:
2371 915 492 262
SpeI A'CTAG_T
Cuts at: 67 192 67
Size: 125 3915
SphI G_CATG'C
Cuts at: 1093 1593 1093
Size: 500 3540
SspI AAT'ATT
Cuts at: 170 170
Size: 4040
StyI C'CwwG_G
Cuts at: 941 1174 1528 941
Size: 233 354 3453
Fragments arranged by size:
3453 354 233
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1623 1756 2215 2424 1623
Size: 133 459 209 3239
Fragments arranged by size:
3239 459 209 133
TatI w'GTAC_w
Cuts at: 315 558 3555 315
Size: 243 2997 800
Fragments arranged by size:
2997 800 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4008
Tth111I GACn'n_nGTC
Cuts at: 1837 1837
Size: 4040
XbaI T'CTAG_A
Cuts at: 1071 1071
Size: 4040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 464 531 1535 464
Size: 67 1004 2969
Fragments arranged by size:
2969 1004 67
XmaI C'CCGG_G
Cuts at: 1060 1060
Size: 4040
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PmeI PpiI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI