Restriction map for
pF1KE0837
(Circular) (Six-base) MAPSORT of: pF1KE0837.seq Check: 3222 from: 1 to: 4067
pF1KE0837 4067 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 1105 1105
Size: 4067
Acc65I G'GTAC_C
Cuts at: 1083 1083
Size: 4067
AclI AA'CG_TT
Cuts at: 3907 3907
Size: 4067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1892 2324 3071 3339 1892
Size: 432 747 268 2620
Fragments arranged by size:
2620 747 432 268
AflIII A'CryG_T
Cuts at: 1419 2523 3500 1419
Size: 1104 977 1986
Fragments arranged by size:
1986 1104 977
AgeI A'CCGG_T
Cuts at: 2455 2455
Size: 4067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 776 776
Size: 4067
AlwNI CAG_nnn'CTG
Cuts at: 553 1372 2939 553
Size: 819 1567 1681
Fragments arranged by size:
1681 1567 819
ApaLI G'TGCA_C
Cuts at: 2837 2837
Size: 4067
ApoI r'AATT_y
Cuts at: 272 558 1039 1071 3420 3676 272
Size: 286 481 32 2349 256 663
Fragments arranged by size:
2349 663 481 286 256 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 4067
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
AvaI C'yCGr_G
Cuts at: 1087 1087
Size: 4067
BamHI G'GATC_C
Cuts at: 1092 1092
Size: 4067
BanI G'GyrC_C
Cuts at: 1083 1497 1745 1780 1083
Size: 414 248 35 3370
Fragments arranged by size:
3370 414 248 35
BanII G_rGCy'C
Cuts at: 807 1081 2111 807
Size: 274 1030 2763
Fragments arranged by size:
2763 1030 274
BbeI G_GCGC'C
Cuts at: 1501 1749 1501
Size: 248 3819
BbvCI CC'TCA_GC
Cuts at: 415 415
Size: 4067
BciVI GTATCCnnnnn_n'
Cuts at: 1965 2726 4067 1965
Size: 761 1341 1965
Fragments arranged by size:
1965 1341 761
BclI T'GATC_A
Cuts at: 1587 1587
Size: 4067
BfrBI ATG'CAT
Cuts at: 181 991 181
Size: 810 3257
BglII A'GATC_T
Cuts at: 1582 3619 1582
Size: 2037 2030
BlpI GC'TnA_GC
Cuts at: 1179 1179
Size: 4067
Bme1580I G_kGCm'C
Cuts at: 1692 1785 2841 1692
Size: 93 1056 2918
Fragments arranged by size:
2918 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 219 520 976 1279 1360 1695 3839 219
Size: 301 456 303 81 335 2144 447
Fragments arranged by size:
2144 456 447 335 303 301 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2434 2434
Size: 4067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 858
Size: 4067
Bpu10I CC'TnA_GC
Cuts at: 415 415
Size: 4067
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 927 1243 2122 2614 2912 927
Size: 316 879 492 298 2082
Fragments arranged by size:
2082 879 492 316 298
BsaI GGTCTCn'nnnn_
Cuts at: 770 3771 770
Size: 3001 1066
BsaAI yAC'GTr
Cuts at: 1420 1420
Size: 4067
BsaBI GATnn'nnATC
Cuts at: 6 1581 3618 6
Size: 1575 2037 455
Fragments arranged by size:
2037 1575 455
BsaHI Gr'CG_yC
Cuts at: 1498 1746 2448 3944 1498
Size: 248 702 1496 1621
Fragments arranged by size:
1621 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1256 1777 2455 2729 2876 3287 1256
Size: 521 678 274 147 411 2036
Fragments arranged by size:
2036 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 823 823
Size: 4067
BseYI C'CCAG_C
Cuts at: 781 1492 2250 2827 3323 781
Size: 711 758 577 496 1525
Fragments arranged by size:
1525 758 711 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 792 142
Size: 650 3417
BsiEI CG_ry'CG
Cuts at: 104 1609 1655 2863 104
Size: 1505 46 1208 1308
Fragments arranged by size:
1505 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1081 1859 2049 2841 1081
Size: 778 190 792 2307
Fragments arranged by size:
2307 792 778 190
BsmI GAATG_Cn'
Cuts at: 181 183 755 181
Size: 2 572 3493
Fragments arranged by size:
3493 572 2
BsmBI CGTCTCn'nnnn_
Cuts at: 886 886
Size: 4067
Bsp1286I G_dGCh'C
Cuts at: 807 1081 1692 1785 1859 2049 2111 2841 807
Size: 274 611 93 74 190 62 730 2033
Fragments arranged by size:
2033 730 611 274 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3287 3287
Size: 4067
BspHI T'CATG_A
Cuts at: 3243 3243
Size: 4067
BspMI ACCTGCnnnn'nnnn_
Cuts at: 418 1117 1331 1633 2014 418
Size: 699 214 302 381 2471
Fragments arranged by size:
2471 699 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2359 2413 3897 4062 50
Size: 2309 54 1484 165 55
Fragments arranged by size:
2309 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 863 1979 863
Size: 1116 2951
BsrFI r'CCGG_y
Cuts at: 2065 2246 2455 2065
Size: 181 209 3677
Fragments arranged by size:
3677 209 181
BssSI C'ACGA_G
Cuts at: 2338 2696 2338
Size: 358 3709
BstAPI GCAn_nnn'nTGC
Cuts at: 1444 1444
Size: 4067
BstBI TT'CG_AA
Cuts at: 2428 2428
Size: 4067
BstYI r'GATC_y
Cuts at: 1 167 1092 1559 1582 1917 2163 3175 3261
Size: 166 925 467 23 335 246 1012 86
Cuts at: 3261 3273 3619 1
Size: 12 346 449
Fragments arranged by size:
1012 925 467 449 346 335 246 166
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4067
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2017 2198 2361 2017
Size: 181 163 3723
Fragments arranged by size:
3723 181 163
BtsI GCAGTG_nn'
Cuts at: 1310 1310
Size: 4067
DraI TTT'AAA
Cuts at: 1066 1066
Size: 4067
DraIII CAC_nnn'GTG
Cuts at: 770 770
Size: 4067
DrdI GACnn_nn'nnGTC
Cuts at: 1773 2631 1773
Size: 858 3209
EaeI y'GGCC_r
Cuts at: 260 1652 1826 2217 2244 3589 260
Size: 1392 174 391 27 1345 738
Fragments arranged by size:
1392 1345 738 391 174 27
EagI C'GGCC_G
Cuts at: 1652 1652
Size: 4067
EarI CTCTTCn'nnn_
Cuts at: 214 2090 2300 214
Size: 1876 210 1981
Fragments arranged by size:
1981 1876 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2275 2585 2731 3877 2275
Size: 310 146 1146 2465
Fragments arranged by size:
2465 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1079 1079
Size: 4067
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 858 1892 2324 3071 3339 858
Size: 1034 432 747 268 1586
Fragments arranged by size:
1586 1034 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1206 1206
Size: 4067
EcoRI G'AATT_C
Cuts at: 1071 1071
Size: 4067
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2086
Size: 4067
FspI TGC'GCA
Cuts at: 1848 1848
Size: 4067
HaeII r_GCGC'y
Cuts at: 1501 1749 2771 3750 1501
Size: 248 1022 979 1818
Fragments arranged by size:
1818 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1909 1939 1941 1971 1909
Size: 30 2 30 4005
Fragments arranged by size:
4005 30 30 2
HincII GTy'rAC
Cuts at: 1106 1106
Size: 4067
KasI G'GCGC_C
Cuts at: 1497 1745 1497
Size: 248 3819
KpnI G_GTAC'C
Cuts at: 1087 1087
Size: 4067
MluI A'CGCG_T
Cuts at: 3500 3500
Size: 4067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 129 1068 2738 2922 129
Size: 939 1670 184 1274
Fragments arranged by size:
1670 1274 939 184
MscI TGG'CCA
Cuts at: 262 1828 262
Size: 1566 2501
MslI CAynn'nnrTG
Cuts at: 2056 2183 2056
Size: 127 3940
MspA1I CmG'CkG
Cuts at: 1178 1492 1852 2865 3110 1178
Size: 314 360 1013 245 2135
Fragments arranged by size:
2135 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2248 2248
Size: 4067
NarI GG'CG_CC
Cuts at: 1498 1746 1498
Size: 248 3819
NcoI C'CATG_G
Cuts at: 107 107
Size: 4067
NgoMIV G'CCGG_C
Cuts at: 2246 2246
Size: 4067
NsiI A_TGCA'T
Cuts at: 183 993 183
Size: 810 3257
NspI r_CATG'y
Cuts at: 646 991 1120 1620 2527 646
Size: 345 129 500 907 2186
Fragments arranged by size:
2186 907 500 345 129
PciI A'CATG_T
Cuts at: 2523 2523
Size: 4067
PfoI T'CCnGG_A
Cuts at: 499 499
Size: 4067
PmeI GTTT'AAAC
Cuts at: 1066 1066
Size: 4067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3206 3238 3206
Size: 32 4035
PstI C_TGCA'G
Cuts at: 1114 1799 1114
Size: 685 3382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4067
PvuII CAG'CTG
Cuts at: 1492 1852 1492
Size: 360 3707
RsrII CG'GwC_CG
Cuts at: 2262 2262
Size: 4067
SacI G_AGCT'C
Cuts at: 1081 1081
Size: 4067
SalI G'TCGA_C
Cuts at: 1104 1104
Size: 4067
SapI GCTCTTCn'nnn_
Cuts at: 2090 2300 2090
Size: 210 3857
SbfI CC_TGCA'GG
Cuts at: 1114 1114
Size: 4067
ScaI AGT'ACT
Cuts at: 3584 3584
Size: 4067
SfcI C'TryA_G
Cuts at: 33 1110 1795 2788 2979 33
Size: 1077 685 993 191 1121
Fragments arranged by size:
1121 1077 993 685 191
SfoI GGC'GCC
Cuts at: 1499 1747 1499
Size: 248 3819
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4067
SmaI CCC'GGG
Cuts at: 1089 1089
Size: 4067
SmlI C'TyrA_G
Cuts at: 942 1222 2137 2629 2891 942
Size: 280 915 492 262 2118
Fragments arranged by size:
2118 915 492 280 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4067
SphI G_CATG'C
Cuts at: 1120 1620 1120
Size: 500 3567
StyI C'CwwG_G
Cuts at: 107 1201 1555 107
Size: 1094 354 2619
Fragments arranged by size:
2619 1094 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 470 1650 1783 2242 2451 470
Size: 1180 133 459 209 2086
Fragments arranged by size:
2086 1180 459 209 133
TatI w'GTAC_w
Cuts at: 3582 3582
Size: 4067
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3584 3616 3584
Size: 32 4035
Tth111I GACn'n_nGTC
Cuts at: 211 1864 211
Size: 1653 2414
XbaI T'CTAG_A
Cuts at: 1098 1098
Size: 4067
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 464 1562 464
Size: 1098 2969
XmaI C'CCGG_G
Cuts at: 1087 1087
Size: 4067
XmnI GAAnn'nnTTC
Cuts at: 181 181
Size: 4067
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbsI BcgI BglI BmgBI BmtI
BsaXI BsiWI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI