Restriction map for
pF1KE0833
(Circular) (Six-base) MAPSORT of: pF1KE0833.seq Check: 2808 from: 1 to: 3785
pF1KE0833 3785 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 823 823
Size: 3785
Acc65I G'GTAC_C
Cuts at: 801 801
Size: 3785
AclI AA'CG_TT
Cuts at: 3625 3625
Size: 3785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 676 1610 2042 2789 3057 676
Size: 934 432 747 268 1404
Fragments arranged by size:
1404 934 747 432 268
AflIII A'CryG_T
Cuts at: 1137 2241 3218 1137
Size: 1104 977 1704
Fragments arranged by size:
1704 1104 977
AgeI A'CCGG_T
Cuts at: 2173 2173
Size: 3785
AlwNI CAG_nnn'CTG
Cuts at: 1090 2657 1090
Size: 1567 2218
ApaLI G'TGCA_C
Cuts at: 2555 2555
Size: 3785
ApoI r'AATT_y
Cuts at: 114 133 789 3138 3394 114
Size: 19 656 2349 256 505
Fragments arranged by size:
2349 656 505 256 19
AseI AT'TA_AT
Cuts at: 20 20
Size: 3785
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
AvaI C'yCGr_G
Cuts at: 805 805
Size: 3785
BamHI G'GATC_C
Cuts at: 810 810
Size: 3785
BanI G'GyrC_C
Cuts at: 801 1215 1463 1498 801
Size: 414 248 35 3088
Fragments arranged by size:
3088 414 248 35
BanII G_rGCy'C
Cuts at: 799 1829 799
Size: 1030 2755
BbeI G_GCGC'C
Cuts at: 1219 1467 1219
Size: 248 3537
BbsI GAAGACnn'nnnn_
Cuts at: 759 759
Size: 3785
BbvCI CC'TCA_GC
Cuts at: 678 724 678
Size: 46 3739
BciVI GTATCCnnnnn_n'
Cuts at: 1683 2444 3785 1683
Size: 761 1341 1683
Fragments arranged by size:
1683 1341 761
BclI T'GATC_A
Cuts at: 1305 1305
Size: 3785
BglII A'GATC_T
Cuts at: 1300 3337 1300
Size: 2037 1748
BlpI GC'TnA_GC
Cuts at: 897 897
Size: 3785
Bme1580I G_kGCm'C
Cuts at: 1410 1503 2559 1410
Size: 93 1056 2636
Fragments arranged by size:
2636 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 640 997 1078 1413 3557 640
Size: 357 81 335 2144 868
Fragments arranged by size:
2144 868 357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 3785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 706 190
Size: 516 3269
Bpu10I CC'TnA_GC
Cuts at: 678 724 678
Size: 46 3739
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 961 1840 2332 2630 961
Size: 879 492 298 2116
Fragments arranged by size:
2116 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3489 3489
Size: 3785
BsaAI yAC'GTr
Cuts at: 1138 1138
Size: 3785
BsaBI GATnn'nnATC
Cuts at: 6 1299 3336 6
Size: 1293 2037 455
Fragments arranged by size:
2037 1293 455
BsaHI Gr'CG_yC
Cuts at: 1216 1464 2166 3662 1216
Size: 248 702 1496 1339
Fragments arranged by size:
1496 1339 702 248
BsaWI w'CCGG_w
Cuts at: 974 1495 2173 2447 2594 3005 974
Size: 521 678 274 147 411 1754
Fragments arranged by size:
1754 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 243 243
Size: 3785
BseYI C'CCAG_C
Cuts at: 144 398 1210 1968 2545 3041 144
Size: 254 812 758 577 496 888
Fragments arranged by size:
888 812 758 577 496 254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 689 411
Size: 278 3507
BsiEI CG_ry'CG
Cuts at: 104 465 1327 1373 2581 104
Size: 361 862 46 1208 1308
Fragments arranged by size:
1308 1208 862 361 46
BsiHKAI G_wGCw'C
Cuts at: 799 1577 1767 2559 799
Size: 778 190 792 2025
Fragments arranged by size:
2025 792 778 190
BsmI GAATG_Cn'
Cuts at: 560 560
Size: 3785
Bsp1286I G_dGCh'C
Cuts at: 799 1410 1503 1577 1767 1829 2559 799
Size: 611 93 74 190 62 730 2025
Fragments arranged by size:
2025 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3005 3005
Size: 3785
BspHI T'CATG_A
Cuts at: 506 2961 506
Size: 2455 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 236 835 1049 1351 1732 236
Size: 599 214 302 381 2289
Fragments arranged by size:
2289 599 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2077 2131 3615 3780 50
Size: 2027 54 1484 165 55
Fragments arranged by size:
2027 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 1697
Size: 3785
BsrFI r'CCGG_y
Cuts at: 1783 1964 2173 1783
Size: 181 209 3395
Fragments arranged by size:
3395 209 181
BsrGI T'GTAC_A
Cuts at: 443 443
Size: 3785
BssSI C'ACGA_G
Cuts at: 2056 2414 2056
Size: 358 3427
BstAPI GCAn_nnn'nTGC
Cuts at: 391 1162 391
Size: 771 3014
BstBI TT'CG_AA
Cuts at: 2146 2146
Size: 3785
BstXI CCAn_nnnn'nTGG
Cuts at: 621 621
Size: 3785
BstYI r'GATC_y
Cuts at: 1 810 1277 1300 1635 1881 2893 2979 2991
Size: 809 467 23 335 246 1012 86 12
Cuts at: 2991 3337 1
Size: 346 449
Fragments arranged by size:
1012 809 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 536 536
Size: 3785
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1735 1916 2079 1735
Size: 181 163 3441
Fragments arranged by size:
3441 181 163
BtsI GCAGTG_nn'
Cuts at: 1028 1028
Size: 3785
DraI TTT'AAA
Cuts at: 784 784
Size: 3785
DraIII CAC_nnn'GTG
Cuts at: 434 434
Size: 3785
DrdI GACnn_nn'nnGTC
Cuts at: 1491 2349 1491
Size: 858 2927
EaeI y'GGCC_r
Cuts at: 1370 1544 1935 1962 3307 1370
Size: 174 391 27 1345 1848
Fragments arranged by size:
1848 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1370 1370
Size: 3785
EarI CTCTTCn'nnn_
Cuts at: 409 1808 2018 409
Size: 1399 210 2176
Fragments arranged by size:
2176 1399 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1993 2303 2449 3595 1993
Size: 310 146 1146 2183
Fragments arranged by size:
2183 1146 310 146
EcoICRI GAG'CTC
Cuts at: 797 797
Size: 3785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 676 706 1610 2042 2789 3057 190
Size: 486 30 904 432 747 268 918
Fragments arranged by size:
918 904 747 486 432 268 30
EcoO109I rG'GnC_Cy
Cuts at: 542 924 542
Size: 382 3403
EcoRI G'AATT_C
Cuts at: 133 789 133
Size: 656 3129
EcoRV GAT'ATC
Cuts at: 642 642
Size: 3785
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1804 1804
Size: 3785
FspI TGC'GCA
Cuts at: 1566 1566
Size: 3785
HaeII r_GCGC'y
Cuts at: 1219 1467 2489 3468 1219
Size: 248 1022 979 1536
Fragments arranged by size:
1536 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 509 541 1627 1657 1659 1689 509
Size: 32 1086 30 2 30 2605
Fragments arranged by size:
2605 1086 32 30 30 2
HincII GTy'rAC
Cuts at: 824 824
Size: 3785
KasI G'GCGC_C
Cuts at: 1215 1463 1215
Size: 248 3537
KpnI G_GTAC'C
Cuts at: 805 805
Size: 3785
MfeI C'AATT_G
Cuts at: 595 595
Size: 3785
MluI A'CGCG_T
Cuts at: 3218 3218
Size: 3785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2456 2640 2456
Size: 184 3601
MscI TGG'CCA
Cuts at: 1546 1546
Size: 3785
MslI CAynn'nnrTG
Cuts at: 217 274 451 505 706 1774 1901 217
Size: 57 177 54 201 1068 127 2101
Fragments arranged by size:
2101 1068 201 177 127 57 54
MspA1I CmG'CkG
Cuts at: 728 896 1210 1570 2583 2828 728
Size: 168 314 360 1013 245 1685
Fragments arranged by size:
1685 1013 360 314 245 168
NaeI GCC'GGC
Cuts at: 1966 1966
Size: 3785
NarI GG'CG_CC
Cuts at: 1216 1464 1216
Size: 248 3537
NcoI C'CATG_G
Cuts at: 536 536
Size: 3785
NgoMIV G'CCGG_C
Cuts at: 1964 1964
Size: 3785
NspI r_CATG'y
Cuts at: 838 1338 2245 838
Size: 500 907 2378
Fragments arranged by size:
2378 907 500
PciI A'CATG_T
Cuts at: 2241 2241
Size: 3785
PflMI CCAn_nnn'nTGG
Cuts at: 420 420
Size: 3785
PmeI GTTT'AAAC
Cuts at: 784 784
Size: 3785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2924 2956 2924
Size: 32 3753
PpuMI rG'GwC_Cy
Cuts at: 542 542
Size: 3785
PstI C_TGCA'G
Cuts at: 832 1517 832
Size: 685 3100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3785
PvuII CAG'CTG
Cuts at: 728 1210 1570 728
Size: 482 360 2943
Fragments arranged by size:
2943 482 360
RsrII CG'GwC_CG
Cuts at: 1980 1980
Size: 3785
SacI G_AGCT'C
Cuts at: 799 799
Size: 3785
SalI G'TCGA_C
Cuts at: 822 822
Size: 3785
SapI GCTCTTCn'nnn_
Cuts at: 409 1808 2018 409
Size: 1399 210 2176
Fragments arranged by size:
2176 1399 210
SbfI CC_TGCA'GG
Cuts at: 832 832
Size: 3785
ScaI AGT'ACT
Cuts at: 3302 3302
Size: 3785
SfcI C'TryA_G
Cuts at: 33 753 828 1513 2506 2697 33
Size: 720 75 685 993 191 1121
Fragments arranged by size:
1121 993 720 685 191 75
SfoI GGC'GCC
Cuts at: 1217 1465 1217
Size: 248 3537
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3785
SmaI CCC'GGG
Cuts at: 807 807
Size: 3785
SmlI C'TyrA_G
Cuts at: 940 1855 2347 2609 940
Size: 915 492 262 2116
Fragments arranged by size:
2116 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3785
SphI G_CATG'C
Cuts at: 838 1338 838
Size: 500 3285
StyI C'CwwG_G
Cuts at: 536 919 1273 536
Size: 383 354 3048
Fragments arranged by size:
3048 383 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1368 1501 1960 2169 1368
Size: 133 459 209 2984
Fragments arranged by size:
2984 459 209 133
TatI w'GTAC_w
Cuts at: 278 443 3300 278
Size: 165 2857 763
Fragments arranged by size:
2857 763 165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3302 3334 3302
Size: 32 3753
Tth111I GACn'n_nGTC
Cuts at: 1582 1582
Size: 3785
XbaI T'CTAG_A
Cuts at: 816 816
Size: 3785
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1280 1280
Size: 3785
XmaI C'CCGG_G
Cuts at: 805 805
Size: 3785
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI EcoNI FseI FspAI HindIII HpaI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI