Restriction map for
pF1KE0831
(Circular) (Six-base) MAPSORT of: pF1KE0831.seq Check: 7719 from: 1 to: 3869
pF1KE0831 3869 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AatII G_ACGT'C
Cuts at: 262 262
Size: 3869
AccI GT'mk_AC
Cuts at: 907 907
Size: 3869
Acc65I G'GTAC_C
Cuts at: 885 885
Size: 3869
AclI AA'CG_TT
Cuts at: 3709 3709
Size: 3869
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1694 2126 2873 3141 631
Size: 1063 432 747 268 1359
Fragments arranged by size:
1359 1063 747 432 268
AflIII A'CryG_T
Cuts at: 1221 2325 3302 1221
Size: 1104 977 1788
Fragments arranged by size:
1788 1104 977
AgeI A'CCGG_T
Cuts at: 2257 2257
Size: 3869
AlwNI CAG_nnn'CTG
Cuts at: 1174 2741 1174
Size: 1567 2302
ApaLI G'TGCA_C
Cuts at: 2639 2639
Size: 3869
ApoI r'AATT_y
Cuts at: 873 3222 3478 873
Size: 2349 256 1264
Fragments arranged by size:
2349 1264 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3869
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
AvaI C'yCGr_G
Cuts at: 889 889
Size: 3869
BamHI G'GATC_C
Cuts at: 894 894
Size: 3869
BanI G'GyrC_C
Cuts at: 632 885 1299 1547 1582 632
Size: 253 414 248 35 2919
Fragments arranged by size:
2919 414 253 248 35
BanII G_rGCy'C
Cuts at: 883 1913 883
Size: 1030 2839
BbeI G_GCGC'C
Cuts at: 636 1303 1551 636
Size: 667 248 2954
Fragments arranged by size:
2954 667 248
BbvCI CC'TCA_GC
Cuts at: 121 121
Size: 3869
BciVI GTATCCnnnnn_n'
Cuts at: 1767 2528 3869 1767
Size: 761 1341 1767
Fragments arranged by size:
1767 1341 761
BclI T'GATC_A
Cuts at: 1389 1389
Size: 3869
BglII A'GATC_T
Cuts at: 1384 3421 1384
Size: 2037 1832
BlpI GC'TnA_GC
Cuts at: 981 981
Size: 3869
Bme1580I G_kGCm'C
Cuts at: 1494 1587 2643 1494
Size: 93 1056 2720
Fragments arranged by size:
2720 1056 93
BmgBI CAC'GTC
Cuts at: 196 196
Size: 3869
BmrI ACTGGGnnnn_n'
Cuts at: 1081 1162 1497 3641 1081
Size: 81 335 2144 1309
Fragments arranged by size:
2144 1309 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2236 2236
Size: 3869
Bpu10I CC'TnA_GC
Cuts at: 121 121
Size: 3869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1924 2416 2714 1045
Size: 879 492 298 2200
Fragments arranged by size:
2200 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3573 3573
Size: 3869
BsaAI yAC'GTr
Cuts at: 1222 1222
Size: 3869
BsaBI GATnn'nnATC
Cuts at: 6 753 1383 3420 6
Size: 747 630 2037 455
Fragments arranged by size:
2037 747 630 455
BsaHI Gr'CG_yC
Cuts at: 259 633 1300 1548 2250 3746 259
Size: 374 667 248 702 1496 382
Fragments arranged by size:
1496 702 667 382 374 248
BsaWI w'CCGG_w
Cuts at: 1058 1579 2257 2531 2678 3089 1058
Size: 521 678 274 147 411 1838
Fragments arranged by size:
1838 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 230 1294 2052 2629 3125 230
Size: 1064 758 577 496 974
Fragments arranged by size:
1064 974 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1411 1457 2665 104
Size: 1307 46 1208 1308
Fragments arranged by size:
1308 1307 1208 46
BsiHKAI G_wGCw'C
Cuts at: 883 1661 1851 2643 883
Size: 778 190 792 2109
Fragments arranged by size:
2109 792 778 190
BsmI GAATG_Cn'
Cuts at: 271 402 271
Size: 131 3738
Bsp1286I G_dGCh'C
Cuts at: 883 1494 1587 1661 1851 1913 2643 883
Size: 611 93 74 190 62 730 2109
Fragments arranged by size:
2109 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3089 3089
Size: 3869
BspHI T'CATG_A
Cuts at: 3045 3045
Size: 3869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 919 1133 1435 1816 919
Size: 214 302 381 2972
Fragments arranged by size:
2972 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2161 2215 3699 3864 50
Size: 2111 54 1484 165 55
Fragments arranged by size:
2111 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1781 1781
Size: 3869
BsrFI r'CCGG_y
Cuts at: 1867 2048 2257 1867
Size: 181 209 3479
Fragments arranged by size:
3479 209 181
BssSI C'ACGA_G
Cuts at: 2140 2498 2140
Size: 358 3511
BstAPI GCAn_nnn'nTGC
Cuts at: 1246 1246
Size: 3869
BstBI TT'CG_AA
Cuts at: 2230 2230
Size: 3869
BstYI r'GATC_y
Cuts at: 1 544 894 1361 1384 1719 1965 2977 3063
Size: 543 350 467 23 335 246 1012 86
Cuts at: 3063 3075 3421 1
Size: 12 346 449
Fragments arranged by size:
1012 543 467 449 350 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 284 284
Size: 3869
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1819 2000 2163 1819
Size: 181 163 3525
Fragments arranged by size:
3525 181 163
BtsI GCAGTG_nn'
Cuts at: 1112 1112
Size: 3869
DraI TTT'AAA
Cuts at: 868 868
Size: 3869
DrdI GACnn_nn'nnGTC
Cuts at: 1575 2433 1575
Size: 858 3011
EaeI y'GGCC_r
Cuts at: 305 1454 1628 2019 2046 3391 305
Size: 1149 174 391 27 1345 783
Fragments arranged by size:
1345 1149 783 391 174 27
EagI C'GGCC_G
Cuts at: 1454 1454
Size: 3869
EarI CTCTTCn'nnn_
Cuts at: 543 1892 2102 543
Size: 1349 210 2310
Fragments arranged by size:
2310 1349 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 612 2077 2387 2533 3679 612
Size: 1465 310 146 1146 802
Fragments arranged by size:
1465 1146 802 310 146
EcoICRI GAG'CTC
Cuts at: 881 881
Size: 3869
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 631 1694 2126 2873 3141 631
Size: 1063 432 747 268 1359
Fragments arranged by size:
1359 1063 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1008 1008
Size: 3869
EcoRI G'AATT_C
Cuts at: 873 873
Size: 3869
EcoRV GAT'ATC
Cuts at: 755 755
Size: 3869
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3869
FspI TGC'GCA
Cuts at: 1650 1650
Size: 3869
HaeII r_GCGC'y
Cuts at: 636 1303 1551 2573 3552 636
Size: 667 248 1022 979 953
Fragments arranged by size:
1022 979 953 667 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1711 1741 1743 1773 1711
Size: 30 2 30 3807
Fragments arranged by size:
3807 30 30 2
HincII GTy'rAC
Cuts at: 908 908
Size: 3869
KasI G'GCGC_C
Cuts at: 632 1299 1547 632
Size: 667 248 2954
Fragments arranged by size:
2954 667 248
KpnI G_GTAC'C
Cuts at: 889 889
Size: 3869
MluI A'CGCG_T
Cuts at: 3302 3302
Size: 3869
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2540 2724 2540
Size: 184 3685
MscI TGG'CCA
Cuts at: 307 1630 307
Size: 1323 2546
MslI CAynn'nnrTG
Cuts at: 709 1858 1985 709
Size: 1149 127 2593
Fragments arranged by size:
2593 1149 127
MspA1I CmG'CkG
Cuts at: 980 1294 1654 2667 2912 980
Size: 314 360 1013 245 1937
Fragments arranged by size:
1937 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2050 2050
Size: 3869
NarI GG'CG_CC
Cuts at: 633 1300 1548 633
Size: 667 248 2954
Fragments arranged by size:
2954 667 248
NcoI C'CATG_G
Cuts at: 284 284
Size: 3869
NgoMIV G'CCGG_C
Cuts at: 2048 2048
Size: 3869
NspI r_CATG'y
Cuts at: 922 1422 2329 922
Size: 500 907 2462
Fragments arranged by size:
2462 907 500
PciI A'CATG_T
Cuts at: 2325 2325
Size: 3869
PmeI GTTT'AAAC
Cuts at: 868 868
Size: 3869
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3008 3040 3008
Size: 32 3837
PstI C_TGCA'G
Cuts at: 916 1601 916
Size: 685 3184
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3869
PvuII CAG'CTG
Cuts at: 1294 1654 1294
Size: 360 3509
RsrII CG'GwC_CG
Cuts at: 2064 2064
Size: 3869
SacI G_AGCT'C
Cuts at: 883 883
Size: 3869
SalI G'TCGA_C
Cuts at: 906 906
Size: 3869
SapI GCTCTTCn'nnn_
Cuts at: 1892 2102 1892
Size: 210 3659
SbfI CC_TGCA'GG
Cuts at: 916 916
Size: 3869
ScaI AGT'ACT
Cuts at: 3386 3386
Size: 3869
SfcI C'TryA_G
Cuts at: 33 242 801 912 1597 2590 2781 33
Size: 209 559 111 685 993 191 1121
Fragments arranged by size:
1121 993 685 559 209 191 111
SfoI GGC'GCC
Cuts at: 634 1301 1549 634
Size: 667 248 2954
Fragments arranged by size:
2954 667 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3869
SmaI CCC'GGG
Cuts at: 891 891
Size: 3869
SmlI C'TyrA_G
Cuts at: 1024 1939 2431 2693 1024
Size: 915 492 262 2200
Fragments arranged by size:
2200 915 492 262
SpeI A'CTAG_T
Cuts at: 67 412 67
Size: 345 3524
SphI G_CATG'C
Cuts at: 922 1422 922
Size: 500 3369
StuI AGG'CCT
Cuts at: 643 643
Size: 3869
StyI C'CwwG_G
Cuts at: 284 1003 1357 284
Size: 719 354 2796
Fragments arranged by size:
2796 719 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 349 373 1452 1585 2044 2253 349
Size: 24 1079 133 459 209 1965
Fragments arranged by size:
1965 1079 459 209 133 24
TatI w'GTAC_w
Cuts at: 3384 3384
Size: 3869
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3386 3418 3386
Size: 32 3837
Tth111I GACn'n_nGTC
Cuts at: 1666 1666
Size: 3869
XbaI T'CTAG_A
Cuts at: 900 900
Size: 3869
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1364 1364
Size: 3869
XmaI C'CCGG_G
Cuts at: 889 889
Size: 3869
XmnI GAAnn'nnTTC
Cuts at: 858 858
Size: 3869
ZraI GAC'GTC
Cuts at: 260 260
Size: 3869
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BcgI BfrBI BglI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI