Restriction map for
pF1KE0830
(Circular) (Six-base) MAPSORT of: pF1KE0830.seq Check: 8960 from: 1 to: 3872
pF1KE0830 3872 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 329 329
Size: 3872
AccI GT'mk_AC
Cuts at: 910 910
Size: 3872
Acc65I G'GTAC_C
Cuts at: 888 888
Size: 3872
AclI AA'CG_TT
Cuts at: 3712 3712
Size: 3872
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1697 2129 2876 3144 508
Size: 1189 432 747 268 1236
Fragments arranged by size:
1236 1189 747 432 268
AflIII A'CryG_T
Cuts at: 1224 2328 3305 1224
Size: 1104 977 1791
Fragments arranged by size:
1791 1104 977
AgeI A'CCGG_T
Cuts at: 2260 2260
Size: 3872
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 337 337
Size: 3872
AlwNI CAG_nnn'CTG
Cuts at: 1177 2744 1177
Size: 1567 2305
ApaLI G'TGCA_C
Cuts at: 2642 2642
Size: 3872
ApoI r'AATT_y
Cuts at: 876 3225 3481 876
Size: 2349 256 1267
Fragments arranged by size:
2349 1267 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3872
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
AvaI C'yCGr_G
Cuts at: 892 892
Size: 3872
BamHI G'GATC_C
Cuts at: 897 897
Size: 3872
BanI G'GyrC_C
Cuts at: 888 1302 1550 1585 888
Size: 414 248 35 3175
Fragments arranged by size:
3175 414 248 35
BanII G_rGCy'C
Cuts at: 886 1916 886
Size: 1030 2842
BbeI G_GCGC'C
Cuts at: 1306 1554 1306
Size: 248 3624
BbsI GAAGACnn'nnnn_
Cuts at: 775 775
Size: 3872
BciVI GTATCCnnnnn_n'
Cuts at: 488 1770 2531 3872 488
Size: 1282 761 1341 488
Fragments arranged by size:
1341 1282 761 488
BclI T'GATC_A
Cuts at: 1392 1392
Size: 3872
BglII A'GATC_T
Cuts at: 141 266 1387 3424 141
Size: 125 1121 2037 589
Fragments arranged by size:
2037 1121 589 125
BlpI GC'TnA_GC
Cuts at: 984 984
Size: 3872
Bme1580I G_kGCm'C
Cuts at: 1497 1590 2646 1497
Size: 93 1056 2723
Fragments arranged by size:
2723 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 392 1084 1165 1500 3644 392
Size: 692 81 335 2144 620
Fragments arranged by size:
2144 692 620 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2239 2239
Size: 3872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1048 1927 2419 2717 1048
Size: 879 492 298 2203
Fragments arranged by size:
2203 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3576 3576
Size: 3872
BsaAI yAC'GTr
Cuts at: 1225 1225
Size: 3872
BsaBI GATnn'nnATC
Cuts at: 6 1386 3423 6
Size: 1380 2037 455
Fragments arranged by size:
2037 1380 455
BsaHI Gr'CG_yC
Cuts at: 1303 1551 2253 3749 1303
Size: 248 702 1496 1426
Fragments arranged by size:
1496 1426 702 248
BsaWI w'CCGG_w
Cuts at: 1061 1582 2260 2534 2681 3092 1061
Size: 521 678 274 147 411 1841
Fragments arranged by size:
1841 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1297 2055 2632 3128 1297
Size: 758 577 496 2041
Fragments arranged by size:
2041 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 307
Size: 3872
BsiEI CG_ry'CG
Cuts at: 104 1414 1460 2668 104
Size: 1310 46 1208 1308
Fragments arranged by size:
1310 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 886 1664 1854 2646 886
Size: 778 190 792 2112
Fragments arranged by size:
2112 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 886 1497 1590 1664 1854 1916 2646 886
Size: 611 93 74 190 62 730 2112
Fragments arranged by size:
2112 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3092 3092
Size: 3872
BspHI T'CATG_A
Cuts at: 3048 3048
Size: 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 329 922 1136 1438 1819 329
Size: 593 214 302 381 2382
Fragments arranged by size:
2382 593 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2164 2218 3702 3867 50
Size: 2114 54 1484 165 55
Fragments arranged by size:
2114 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 290 764 1784 290
Size: 474 1020 2378
Fragments arranged by size:
2378 1020 474
BsrFI r'CCGG_y
Cuts at: 1870 2051 2260 1870
Size: 181 209 3482
Fragments arranged by size:
3482 209 181
BssSI C'ACGA_G
Cuts at: 2143 2501 2143
Size: 358 3514
BstAPI GCAn_nnn'nTGC
Cuts at: 1249 1249
Size: 3872
BstBI TT'CG_AA
Cuts at: 2233 2233
Size: 3872
BstYI r'GATC_y
Cuts at: 1 141 266 897 1364 1387 1722 1968 2980
Size: 140 125 631 467 23 335 246 1012
Cuts at: 2980 3066 3078 3424 1
Size: 86 12 346 449
Fragments arranged by size:
1012 631 467 449 346 335 246 140
125 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1822 2003 2166 1822
Size: 181 163 3528
Fragments arranged by size:
3528 181 163
BtsI GCAGTG_nn'
Cuts at: 1115 1115
Size: 3872
DraI TTT'AAA
Cuts at: 871 871
Size: 3872
DrdI GACnn_nn'nnGTC
Cuts at: 1578 2436 1578
Size: 858 3014
EaeI y'GGCC_r
Cuts at: 1457 1631 2022 2049 3394 1457
Size: 174 391 27 1345 1935
Fragments arranged by size:
1935 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1457 1457
Size: 3872
EarI CTCTTCn'nnn_
Cuts at: 703 757 1895 2105 703
Size: 54 1138 210 2470
Fragments arranged by size:
2470 1138 210 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2080 2390 2536 3682 2080
Size: 310 146 1146 2270
Fragments arranged by size:
2270 1146 310 146
EcoICRI GAG'CTC
Cuts at: 884 884
Size: 3872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1697 2129 2876 3144 508
Size: 1189 432 747 268 1236
Fragments arranged by size:
1236 1189 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1011 1011
Size: 3872
EcoRI G'AATT_C
Cuts at: 876 876
Size: 3872
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1891 1891
Size: 3872
FspI TGC'GCA
Cuts at: 1653 1653
Size: 3872
HaeII r_GCGC'y
Cuts at: 1306 1554 2576 3555 1306
Size: 248 1022 979 1623
Fragments arranged by size:
1623 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1714 1744 1746 1776 1714
Size: 30 2 30 3810
Fragments arranged by size:
3810 30 30 2
HincII GTy'rAC
Cuts at: 911 911
Size: 3872
KasI G'GCGC_C
Cuts at: 1302 1550 1302
Size: 248 3624
KpnI G_GTAC'C
Cuts at: 892 892
Size: 3872
MfeI C'AATT_G
Cuts at: 847 847
Size: 3872
MluI A'CGCG_T
Cuts at: 3305 3305
Size: 3872
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2727 2543
Size: 184 3688
MscI TGG'CCA
Cuts at: 1633 1633
Size: 3872
MslI CAynn'nnrTG
Cuts at: 1861 1988 1861
Size: 127 3745
MspA1I CmG'CkG
Cuts at: 983 1297 1657 2670 2915 983
Size: 314 360 1013 245 1940
Fragments arranged by size:
1940 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 3872
NarI GG'CG_CC
Cuts at: 1303 1551 1303
Size: 248 3624
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 3872
NspI r_CATG'y
Cuts at: 798 925 1425 2332 798
Size: 127 500 907 2338
Fragments arranged by size:
2338 907 500 127
PciI A'CATG_T
Cuts at: 2328 2328
Size: 3872
PmeI GTTT'AAAC
Cuts at: 871 871
Size: 3872
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3011 3043 3011
Size: 32 3840
PstI C_TGCA'G
Cuts at: 919 1604 919
Size: 685 3187
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3872
PvuII CAG'CTG
Cuts at: 1297 1657 1297
Size: 360 3512
RsrII CG'GwC_CG
Cuts at: 2067 2067
Size: 3872
SacI G_AGCT'C
Cuts at: 886 886
Size: 3872
SalI G'TCGA_C
Cuts at: 909 909
Size: 3872
SapI GCTCTTCn'nnn_
Cuts at: 1895 2105 1895
Size: 210 3662
SbfI CC_TGCA'GG
Cuts at: 919 919
Size: 3872
ScaI AGT'ACT
Cuts at: 463 3389 463
Size: 2926 946
SfcI C'TryA_G
Cuts at: 33 651 915 1600 2593 2784 33
Size: 618 264 685 993 191 1121
Fragments arranged by size:
1121 993 685 618 264 191
SfoI GGC'GCC
Cuts at: 1304 1552 1304
Size: 248 3624
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3872
SmaI CCC'GGG
Cuts at: 894 894
Size: 3872
SmlI C'TyrA_G
Cuts at: 1027 1942 2434 2696 1027
Size: 915 492 262 2203
Fragments arranged by size:
2203 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3872
SphI G_CATG'C
Cuts at: 925 1425 925
Size: 500 3372
StuI AGG'CCT
Cuts at: 545 545
Size: 3872
StyI C'CwwG_G
Cuts at: 252 1006 1360 252
Size: 754 354 2764
Fragments arranged by size:
2764 754 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1455 1588 2047 2256 1455
Size: 133 459 209 3071
Fragments arranged by size:
3071 459 209 133
TatI w'GTAC_w
Cuts at: 379 461 3387 379
Size: 82 2926 864
Fragments arranged by size:
2926 864 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3389 3421 3389
Size: 32 3840
Tth111I GACn'n_nGTC
Cuts at: 1669 1669
Size: 3872
XbaI T'CTAG_A
Cuts at: 903 903
Size: 3872
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1367 1367
Size: 3872
XmaI C'CCGG_G
Cuts at: 892 892
Size: 3872
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI