Restriction map for
pF1KE0829
(Circular) (Six-base) MAPSORT of: pF1KE0829.seq Check: 6171 from: 1 to: 3941
pF1KE0829 3941 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 979 979
Size: 3941
Acc65I G'GTAC_C
Cuts at: 957 957
Size: 3941
AclI AA'CG_TT
Cuts at: 3781 3781
Size: 3941
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 1766 2198 2945 3213 658
Size: 1108 432 747 268 1386
Fragments arranged by size:
1386 1108 747 432 268
AflIII A'CryG_T
Cuts at: 1293 2397 3374 1293
Size: 1104 977 1860
Fragments arranged by size:
1860 1104 977
AgeI A'CCGG_T
Cuts at: 2329 2329
Size: 3941
AleI CACnn'nnGTG
Cuts at: 151 151
Size: 3941
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 532 532
Size: 3941
AlwNI CAG_nnn'CTG
Cuts at: 1246 2813 1246
Size: 1567 2374
ApaLI G'TGCA_C
Cuts at: 2711 2711
Size: 3941
ApoI r'AATT_y
Cuts at: 945 3294 3550 945
Size: 2349 256 1336
Fragments arranged by size:
2349 1336 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3941
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
AvaI C'yCGr_G
Cuts at: 961 961
Size: 3941
AvrII C'CTAG_G
Cuts at: 171 171
Size: 3941
BamHI G'GATC_C
Cuts at: 966 966
Size: 3941
BanI G'GyrC_C
Cuts at: 111 957 1371 1619 1654 111
Size: 846 414 248 35 2398
Fragments arranged by size:
2398 846 414 248 35
BanII G_rGCy'C
Cuts at: 757 955 1985 757
Size: 198 1030 2713
Fragments arranged by size:
2713 1030 198
BbeI G_GCGC'C
Cuts at: 1375 1623 1375
Size: 248 3693
BbsI GAAGACnn'nnnn_
Cuts at: 210 403 210
Size: 193 3748
BciVI GTATCCnnnnn_n'
Cuts at: 1839 2600 3941 1839
Size: 761 1341 1839
Fragments arranged by size:
1839 1341 761
BclI T'GATC_A
Cuts at: 1461 1461
Size: 3941
BfrBI ATG'CAT
Cuts at: 909 909
Size: 3941
BglII A'GATC_T
Cuts at: 1456 3493 1456
Size: 2037 1904
BlpI GC'TnA_GC
Cuts at: 1053 1053
Size: 3941
Bme1580I G_kGCm'C
Cuts at: 1566 1659 2715 1566
Size: 93 1056 2792
Fragments arranged by size:
2792 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1153 1234 1569 3713 1153
Size: 81 335 2144 1381
Fragments arranged by size:
2144 1381 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 3941
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 587
Size: 3941
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1117 1996 2488 2786 1117
Size: 879 492 298 2272
Fragments arranged by size:
2272 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3645 3645
Size: 3941
BsaAI yAC'GTr
Cuts at: 1294 1294
Size: 3941
BsaBI GATnn'nnATC
Cuts at: 6 1455 3492 6
Size: 1449 2037 455
Fragments arranged by size:
2037 1449 455
BsaHI Gr'CG_yC
Cuts at: 1372 1620 2322 3818 1372
Size: 248 702 1496 1495
Fragments arranged by size:
1496 1495 702 248
BsaWI w'CCGG_w
Cuts at: 1130 1651 2329 2603 2750 3161 1130
Size: 521 678 274 147 411 1910
Fragments arranged by size:
1910 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1366 2124 2701 3197 1366
Size: 758 577 496 2110
Fragments arranged by size:
2110 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1483 1529 2737 104
Size: 1379 46 1208 1308
Fragments arranged by size:
1379 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 955 1733 1923 2715 955
Size: 778 190 792 2181
Fragments arranged by size:
2181 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 757 955 1566 1659 1733 1923 1985 2715 757
Size: 198 611 93 74 190 62 730 1983
Fragments arranged by size:
1983 730 611 198 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3161 3161
Size: 3941
BspHI T'CATG_A
Cuts at: 3117 3117
Size: 3941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 991 1205 1507 1888 991
Size: 214 302 381 3044
Fragments arranged by size:
3044 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2233 2287 3771 3936 50
Size: 2183 54 1484 165 55
Fragments arranged by size:
2183 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1853 1853
Size: 3941
BsrFI r'CCGG_y
Cuts at: 1939 2120 2329 1939
Size: 181 209 3551
Fragments arranged by size:
3551 209 181
BsrGI T'GTAC_A
Cuts at: 902 902
Size: 3941
BssSI C'ACGA_G
Cuts at: 2212 2570 2212
Size: 358 3583
BstAPI GCAn_nnn'nTGC
Cuts at: 1318 1318
Size: 3941
BstBI TT'CG_AA
Cuts at: 2302 2302
Size: 3941
BstYI r'GATC_y
Cuts at: 1 121 966 1433 1456 1791 2037 3049 3135
Size: 120 845 467 23 335 246 1012 86
Cuts at: 3135 3147 3493 1
Size: 12 346 449
Fragments arranged by size:
1012 845 467 449 346 335 246 120
86 23 12
BtgI C'CryG_G
Cuts at: 107 125 107
Size: 18 3923
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1891 2072 2235 1891
Size: 181 163 3597
Fragments arranged by size:
3597 181 163
BtsI GCAGTG_nn'
Cuts at: 1184 1184
Size: 3941
DraI TTT'AAA
Cuts at: 940 940
Size: 3941
DrdI GACnn_nn'nnGTC
Cuts at: 1647 2505 1647
Size: 858 3083
EaeI y'GGCC_r
Cuts at: 155 500 1526 1700 2091 2118 3463 155
Size: 345 1026 174 391 27 1345 633
Fragments arranged by size:
1345 1026 633 391 345 174 27
EagI C'GGCC_G
Cuts at: 1526 1526
Size: 3941
EarI CTCTTCn'nnn_
Cuts at: 883 1964 2174 883
Size: 1081 210 2650
Fragments arranged by size:
2650 1081 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2149 2459 2605 3751 2149
Size: 310 146 1146 2339
Fragments arranged by size:
2339 1146 310 146
EcoICRI GAG'CTC
Cuts at: 953 953
Size: 3941
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 587 658 1766 2198 2945 3213 587
Size: 71 1108 432 747 268 1315
Fragments arranged by size:
1315 1108 747 432 268 71
EcoO109I rG'GnC_Cy
Cuts at: 440 1080 440
Size: 640 3301
EcoRI G'AATT_C
Cuts at: 945 945
Size: 3941
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1960 1960
Size: 3941
FspI TGC'GCA
Cuts at: 1722 1722
Size: 3941
HaeII r_GCGC'y
Cuts at: 1375 1623 2645 3624 1375
Size: 248 1022 979 1692
Fragments arranged by size:
1692 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1783 1813 1815 1845 1783
Size: 30 2 30 3879
Fragments arranged by size:
3879 30 30 2
HincII GTy'rAC
Cuts at: 980 980
Size: 3941
KasI G'GCGC_C
Cuts at: 1371 1619 1371
Size: 248 3693
KpnI G_GTAC'C
Cuts at: 961 961
Size: 3941
MluI A'CGCG_T
Cuts at: 3374 3374
Size: 3941
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 532 722 2612 2796 532
Size: 190 1890 184 1677
Fragments arranged by size:
1890 1677 190 184
MscI TGG'CCA
Cuts at: 157 502 1702 157
Size: 345 1200 2396
Fragments arranged by size:
2396 1200 345
MslI CAynn'nnrTG
Cuts at: 130 151 301 1930 2057 130
Size: 21 150 1629 127 2014
Fragments arranged by size:
2014 1629 150 127 21
MspA1I CmG'CkG
Cuts at: 868 1052 1366 1726 2739 2984 868
Size: 184 314 360 1013 245 1825
Fragments arranged by size:
1825 1013 360 314 245 184
NaeI GCC'GGC
Cuts at: 2122 2122
Size: 3941
NarI GG'CG_CC
Cuts at: 1372 1620 1372
Size: 248 3693
NcoI C'CATG_G
Cuts at: 107 125 107
Size: 18 3923
NgoMIV G'CCGG_C
Cuts at: 2120 2120
Size: 3941
NsiI A_TGCA'T
Cuts at: 911 911
Size: 3941
NspI r_CATG'y
Cuts at: 909 994 1494 2401 909
Size: 85 500 907 2449
Fragments arranged by size:
2449 907 500 85
PciI A'CATG_T
Cuts at: 2397 2397
Size: 3941
PmeI GTTT'AAAC
Cuts at: 940 940
Size: 3941
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 133 165 3080 3112 133
Size: 32 2915 32 962
Fragments arranged by size:
2915 962 32 32
PstI C_TGCA'G
Cuts at: 200 521 988 1673 200
Size: 321 467 685 2468
Fragments arranged by size:
2468 685 467 321
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3941
PvuII CAG'CTG
Cuts at: 868 1366 1726 868
Size: 498 360 3083
Fragments arranged by size:
3083 498 360
RsrII CG'GwC_CG
Cuts at: 2136 2136
Size: 3941
SacI G_AGCT'C
Cuts at: 955 955
Size: 3941
SalI G'TCGA_C
Cuts at: 978 978
Size: 3941
SapI GCTCTTCn'nnn_
Cuts at: 1964 2174 1964
Size: 210 3731
SbfI CC_TGCA'GG
Cuts at: 988 988
Size: 3941
ScaI AGT'ACT
Cuts at: 523 3458 523
Size: 2935 1006
SfcI C'TryA_G
Cuts at: 33 196 517 984 1669 2662 2853 33
Size: 163 321 467 685 993 191 1121
Fragments arranged by size:
1121 993 685 467 321 191 163
SfoI GGC'GCC
Cuts at: 1373 1621 1373
Size: 248 3693
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3941
SmaI CCC'GGG
Cuts at: 963 963
Size: 3941
SmlI C'TyrA_G
Cuts at: 1096 2011 2503 2765 1096
Size: 915 492 262 2272
Fragments arranged by size:
2272 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3941
SphI G_CATG'C
Cuts at: 994 1494 994
Size: 500 3441
StuI AGG'CCT
Cuts at: 838 838
Size: 3941
StyI C'CwwG_G
Cuts at: 107 125 171 364 833 1075 1429 107
Size: 18 46 193 469 242 354 2619
Fragments arranged by size:
2619 469 354 242 193 46 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 315 1524 1657 2116 2325 315
Size: 1209 133 459 209 1931
Fragments arranged by size:
1931 1209 459 209 133
TatI w'GTAC_w
Cuts at: 305 521 902 3456 305
Size: 216 381 2554 790
Fragments arranged by size:
2554 790 381 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3458 3490 3458
Size: 32 3909
Tth111I GACn'n_nGTC
Cuts at: 1738 1738
Size: 3941
XbaI T'CTAG_A
Cuts at: 972 972
Size: 3941
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1436 1436
Size: 3941
XmaI C'CCGG_G
Cuts at: 961 961
Size: 3941
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I BsaXI
BseRI BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI