Restriction map for
pF1KE0827
(Circular) (Six-base) MAPSORT of: pF1KE0827.seq Check: 6231 from: 1 to: 3773
pF1KE0827 3773 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 529 811 529
Size: 282 3491
Acc65I G'GTAC_C
Cuts at: 789 789
Size: 3773
AclI AA'CG_TT
Cuts at: 3613 3613
Size: 3773
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1598 2030 2777 3045 1598
Size: 432 747 268 2326
Fragments arranged by size:
2326 747 432 268
AflIII A'CryG_T
Cuts at: 356 677 1125 2229 3206 356
Size: 321 448 1104 977 923
Fragments arranged by size:
1104 977 923 448 321
AgeI A'CCGG_T
Cuts at: 2161 2161
Size: 3773
AlwNI CAG_nnn'CTG
Cuts at: 1078 2645 1078
Size: 1567 2206
ApaLI G'TGCA_C
Cuts at: 381 2543 381
Size: 2162 1611
ApoI r'AATT_y
Cuts at: 622 687 777 3126 3382 622
Size: 65 90 2349 256 1013
Fragments arranged by size:
2349 1013 256 90 65
AseI AT'TA_AT
Cuts at: 20 20
Size: 3773
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
AvaI C'yCGr_G
Cuts at: 793 793
Size: 3773
BamHI G'GATC_C
Cuts at: 798 798
Size: 3773
BanI G'GyrC_C
Cuts at: 789 1203 1451 1486 789
Size: 414 248 35 3076
Fragments arranged by size:
3076 414 248 35
BanII G_rGCy'C
Cuts at: 787 1817 787
Size: 1030 2743
BbeI G_GCGC'C
Cuts at: 1207 1455 1207
Size: 248 3525
BbsI GAAGACnn'nnnn_
Cuts at: 304 304
Size: 3773
BciVI GTATCCnnnnn_n'
Cuts at: 1671 2432 3773 1671
Size: 761 1341 1671
Fragments arranged by size:
1671 1341 761
BclI T'GATC_A
Cuts at: 1293 1293
Size: 3773
BglII A'GATC_T
Cuts at: 149 1288 3325 149
Size: 1139 2037 597
Fragments arranged by size:
2037 1139 597
BlpI GC'TnA_GC
Cuts at: 885 885
Size: 3773
Bme1580I G_kGCm'C
Cuts at: 385 1398 1491 2547 385
Size: 1013 93 1056 1611
Fragments arranged by size:
1611 1056 1013 93
BmrI ACTGGGnnnn_n'
Cuts at: 985 1066 1401 3545 985
Size: 81 335 2144 1213
Fragments arranged by size:
2144 1213 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 775 2140 775
Size: 1365 2408
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 287
Size: 3773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 949 1828 2320 2618 949
Size: 879 492 298 2104
Fragments arranged by size:
2104 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3477 3477
Size: 3773
BsaAI yAC'GTr
Cuts at: 186 1126 186
Size: 940 2833
BsaBI GATnn'nnATC
Cuts at: 6 1287 3324 6
Size: 1281 2037 455
Fragments arranged by size:
2037 1281 455
BsaHI Gr'CG_yC
Cuts at: 1204 1452 2154 3650 1204
Size: 248 702 1496 1327
Fragments arranged by size:
1496 1327 702 248
BsaWI w'CCGG_w
Cuts at: 962 1483 2161 2435 2582 2993 962
Size: 521 678 274 147 411 1742
Fragments arranged by size:
1742 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 751 751
Size: 3773
BseYI C'CCAG_C
Cuts at: 1198 1956 2533 3029 1198
Size: 758 577 496 1942
Fragments arranged by size:
1942 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 190
Size: 3773
BsiEI CG_ry'CG
Cuts at: 104 1315 1361 2569 104
Size: 1211 46 1208 1308
Fragments arranged by size:
1308 1211 1208 46
BsiHKAI G_wGCw'C
Cuts at: 385 787 1565 1755 2547 385
Size: 402 778 190 792 1611
Fragments arranged by size:
1611 792 778 402 190
Bsp1286I G_dGCh'C
Cuts at: 385 787 1398 1491 1565 1755 1817 2547 385
Size: 402 611 93 74 190 62 730 1611
Fragments arranged by size:
1611 730 611 402 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2993 2993
Size: 3773
BspHI T'CATG_A
Cuts at: 221 2949 221
Size: 2728 1045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 541 823 1037 1339 1720 541
Size: 282 214 302 381 2594
Fragments arranged by size:
2594 381 302 282 214
BsrBI CCG'CTC
Cuts at: 50 2065 2119 3603 3768 50
Size: 2015 54 1484 165 55
Fragments arranged by size:
2015 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 437 1685 437
Size: 1248 2525
BsrFI r'CCGG_y
Cuts at: 1771 1952 2161 1771
Size: 181 209 3383
Fragments arranged by size:
3383 209 181
BsrGI T'GTAC_A
Cuts at: 305 305
Size: 3773
BssSI C'ACGA_G
Cuts at: 2044 2402 2044
Size: 358 3415
BstAPI GCAn_nnn'nTGC
Cuts at: 1150 1150
Size: 3773
BstBI TT'CG_AA
Cuts at: 2134 2134
Size: 3773
BstXI CCAn_nnnn'nTGG
Cuts at: 136 136
Size: 3773
BstYI r'GATC_y
Cuts at: 1 149 798 1265 1288 1623 1869 2881 2967
Size: 148 649 467 23 335 246 1012 86
Cuts at: 2967 2979 3325 1
Size: 12 346 449
Fragments arranged by size:
1012 649 467 449 346 335 246 148
86 23 12
BtgI C'CryG_G
Cuts at: 107 551 107
Size: 444 3329
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1723 1904 2067 1723
Size: 181 163 3429
Fragments arranged by size:
3429 181 163
BtsI GCAGTG_nn'
Cuts at: 202 1016 202
Size: 814 2959
DraI TTT'AAA
Cuts at: 772 772
Size: 3773
DrdI GACnn_nn'nnGTC
Cuts at: 1479 2337 1479
Size: 858 2915
EaeI y'GGCC_r
Cuts at: 188 257 1358 1532 1923 1950 3295 188
Size: 69 1101 174 391 27 1345 666
Fragments arranged by size:
1345 1101 666 391 174 69 27
EagI C'GGCC_G
Cuts at: 1358 1358
Size: 3773
EarI CTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1981 2291 2437 3583 1981
Size: 310 146 1146 2171
Fragments arranged by size:
2171 1146 310 146
EcoICRI GAG'CTC
Cuts at: 785 785
Size: 3773
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 287 1598 2030 2777 3045 287
Size: 1311 432 747 268 1015
Fragments arranged by size:
1311 1015 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 912 912
Size: 3773
EcoRI G'AATT_C
Cuts at: 777 777
Size: 3773
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1792 1792
Size: 3773
FspI TGC'GCA
Cuts at: 1554 1554
Size: 3773
HaeII r_GCGC'y
Cuts at: 1207 1455 2477 3456 1207
Size: 248 1022 979 1524
Fragments arranged by size:
1524 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1615 1645 1647 1677 1615
Size: 30 2 30 3711
Fragments arranged by size:
3711 30 30 2
HincII GTy'rAC
Cuts at: 812 812
Size: 3773
KasI G'GCGC_C
Cuts at: 1203 1451 1203
Size: 248 3525
KpnI G_GTAC'C
Cuts at: 793 793
Size: 3773
MluI A'CGCG_T
Cuts at: 3206 3206
Size: 3773
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2444 2628 2444
Size: 184 3589
MscI TGG'CCA
Cuts at: 190 259 1534 190
Size: 69 1275 2429
Fragments arranged by size:
2429 1275 69
MslI CAynn'nnrTG
Cuts at: 550 655 1762 1889 550
Size: 105 1107 127 2434
Fragments arranged by size:
2434 1107 127 105
MspA1I CmG'CkG
Cuts at: 884 1198 1558 2571 2816 884
Size: 314 360 1013 245 1841
Fragments arranged by size:
1841 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1954 1954
Size: 3773
NarI GG'CG_CC
Cuts at: 1204 1452 1204
Size: 248 3525
NcoI C'CATG_G
Cuts at: 107 107
Size: 3773
NgoMIV G'CCGG_C
Cuts at: 1952 1952
Size: 3773
NspI r_CATG'y
Cuts at: 360 826 1326 2233 360
Size: 466 500 907 1900
Fragments arranged by size:
1900 907 500 466
PciI A'CATG_T
Cuts at: 356 2229 356
Size: 1873 1900
PmeI GTTT'AAAC
Cuts at: 772 772
Size: 3773
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2912 2944 2912
Size: 32 3741
PstI C_TGCA'G
Cuts at: 538 820 1505 538
Size: 282 685 2806
Fragments arranged by size:
2806 685 282
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3773
PvuII CAG'CTG
Cuts at: 1198 1558 1198
Size: 360 3413
RsrII CG'GwC_CG
Cuts at: 1968 1968
Size: 3773
SacI G_AGCT'C
Cuts at: 787 787
Size: 3773
SalI G'TCGA_C
Cuts at: 810 810
Size: 3773
SapI GCTCTTCn'nnn_
Cuts at: 1796 2006 1796
Size: 210 3563
SbfI CC_TGCA'GG
Cuts at: 820 820
Size: 3773
ScaI AGT'ACT
Cuts at: 3290 3290
Size: 3773
SfcI C'TryA_G
Cuts at: 33 410 534 737 816 1501 2494 2685 33
Size: 377 124 203 79 685 993 191 1121
Fragments arranged by size:
1121 993 685 377 203 191 124 79
SfoI GGC'GCC
Cuts at: 1205 1453 1205
Size: 248 3525
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3773
SmaI CCC'GGG
Cuts at: 795 795
Size: 3773
SmlI C'TyrA_G
Cuts at: 928 1843 2335 2597 928
Size: 915 492 262 2104
Fragments arranged by size:
2104 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3773
SphI G_CATG'C
Cuts at: 826 1326 826
Size: 500 3273
StuI AGG'CCT
Cuts at: 404 404
Size: 3773
StyI C'CwwG_G
Cuts at: 107 907 1261 107
Size: 800 354 2619
Fragments arranged by size:
2619 800 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 669 1356 1489 1948 2157 669
Size: 687 133 459 209 2285
Fragments arranged by size:
2285 687 459 209 133
TatI w'GTAC_w
Cuts at: 305 3288 305
Size: 2983 790
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 364 396 3290 3322 364
Size: 32 2894 32 815
Fragments arranged by size:
2894 815 32 32
Tth111I GACn'n_nGTC
Cuts at: 1570 1570
Size: 3773
XbaI T'CTAG_A
Cuts at: 804 804
Size: 3773
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 136 246 1268 136
Size: 110 1022 2641
Fragments arranged by size:
2641 1022 110
XmaI C'CCGG_G
Cuts at: 793 793
Size: 3773
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI