Restriction map for
pF1KE0826
(Circular) (Six-base) MAPSORT of: pF1KE0826.seq Check: 7550 from: 1 to: 3866
pF1KE0826 3866 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 904 904
Size: 3866
Acc65I G'GTAC_C
Cuts at: 882 882
Size: 3866
AclI AA'CG_TT
Cuts at: 3706 3706
Size: 3866
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 815 1691 2123 2870 3138 799
Size: 16 876 432 747 268 1527
Fragments arranged by size:
1527 876 747 432 268 16
AflIII A'CryG_T
Cuts at: 1218 2322 3299 1218
Size: 1104 977 1785
Fragments arranged by size:
1785 1104 977
AgeI A'CCGG_T
Cuts at: 2254 2254
Size: 3866
AlwNI CAG_nnn'CTG
Cuts at: 1171 2738 1171
Size: 1567 2299
ApaLI G'TGCA_C
Cuts at: 2636 2636
Size: 3866
ApoI r'AATT_y
Cuts at: 870 3219 3475 870
Size: 2349 256 1261
Fragments arranged by size:
2349 1261 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3866
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
AvaI C'yCGr_G
Cuts at: 886 886
Size: 3866
BamHI G'GATC_C
Cuts at: 891 891
Size: 3866
BanI G'GyrC_C
Cuts at: 882 1296 1544 1579 882
Size: 414 248 35 3169
Fragments arranged by size:
3169 414 248 35
BanII G_rGCy'C
Cuts at: 880 1910 880
Size: 1030 2836
BbeI G_GCGC'C
Cuts at: 1300 1548 1300
Size: 248 3618
BciVI GTATCCnnnnn_n'
Cuts at: 1764 2525 3866 1764
Size: 761 1341 1764
Fragments arranged by size:
1764 1341 761
BclI T'GATC_A
Cuts at: 1386 1386
Size: 3866
BglII A'GATC_T
Cuts at: 1381 3418 1381
Size: 2037 1829
BlpI GC'TnA_GC
Cuts at: 978 978
Size: 3866
Bme1580I G_kGCm'C
Cuts at: 731 1491 1584 2640 731
Size: 760 93 1056 1957
Fragments arranged by size:
1957 1056 760 93
BmrI ACTGGGnnnn_n'
Cuts at: 1078 1159 1494 3638 1078
Size: 81 335 2144 1306
Fragments arranged by size:
2144 1306 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 3866
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 1042 1921 2413 2711 237
Size: 805 879 492 298 1392
Fragments arranged by size:
1392 879 805 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3570 3570
Size: 3866
BsaAI yAC'GTr
Cuts at: 1219 1219
Size: 3866
BsaBI GATnn'nnATC
Cuts at: 6 346 739 1380 3417 6
Size: 340 393 641 2037 455
Fragments arranged by size:
2037 641 455 393 340
BsaHI Gr'CG_yC
Cuts at: 1297 1545 2247 3743 1297
Size: 248 702 1496 1420
Fragments arranged by size:
1496 1420 702 248
BsaWI w'CCGG_w
Cuts at: 1055 1576 2254 2528 2675 3086 1055
Size: 521 678 274 147 411 1835
Fragments arranged by size:
1835 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 144 1291 2049 2626 3122 144
Size: 1147 758 577 496 888
Fragments arranged by size:
1147 888 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1408 1454 2662 104
Size: 1304 46 1208 1308
Fragments arranged by size:
1308 1304 1208 46
BsiHKAI G_wGCw'C
Cuts at: 880 1658 1848 2640 880
Size: 778 190 792 2106
Fragments arranged by size:
2106 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 731 880 1491 1584 1658 1848 1910 2640 731
Size: 149 611 93 74 190 62 730 1957
Fragments arranged by size:
1957 730 611 190 149 93 74 62
BspEI T'CCGG_A
Cuts at: 3086 3086
Size: 3866
BspHI T'CATG_A
Cuts at: 506 3042 506
Size: 2536 1330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 916 1130 1432 1813 916
Size: 214 302 381 2969
Fragments arranged by size:
2969 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2158 2212 3696 3861 50
Size: 2108 54 1484 165 55
Fragments arranged by size:
2108 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1778 1778
Size: 3866
BsrFI r'CCGG_y
Cuts at: 1864 2045 2254 1864
Size: 181 209 3476
Fragments arranged by size:
3476 209 181
BssSI C'ACGA_G
Cuts at: 2137 2495 2137
Size: 358 3508
BstAPI GCAn_nnn'nTGC
Cuts at: 1243 1243
Size: 3866
BstBI TT'CG_AA
Cuts at: 2227 2227
Size: 3866
BstYI r'GATC_y
Cuts at: 1 782 891 1358 1381 1716 1962 2974 3060
Size: 781 109 467 23 335 246 1012 86
Cuts at: 3060 3072 3418 1
Size: 12 346 449
Fragments arranged by size:
1012 781 467 449 346 335 246 109
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3866
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1816 1997 2160 1816
Size: 181 163 3522
Fragments arranged by size:
3522 181 163
BtsI GCAGTG_nn'
Cuts at: 1109 1109
Size: 3866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 616 651 616
Size: 35 3831
DraI TTT'AAA
Cuts at: 865 865
Size: 3866
DrdI GACnn_nn'nnGTC
Cuts at: 1572 2430 1572
Size: 858 3008
EaeI y'GGCC_r
Cuts at: 110 1451 1625 2016 2043 3388 110
Size: 1341 174 391 27 1345 588
Fragments arranged by size:
1345 1341 588 391 174 27
EagI C'GGCC_G
Cuts at: 1451 1451
Size: 3866
EarI CTCTTCn'nnn_
Cuts at: 1889 2099 1889
Size: 210 3656
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2074 2384 2530 3676 2074
Size: 310 146 1146 2264
Fragments arranged by size:
2264 1146 310 146
EcoICRI GAG'CTC
Cuts at: 878 878
Size: 3866
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 815 1691 2123 2870 3138 799
Size: 16 876 432 747 268 1527
Fragments arranged by size:
1527 876 747 432 268 16
EcoO109I rG'GnC_Cy
Cuts at: 1005 1005
Size: 3866
EcoRI G'AATT_C
Cuts at: 870 870
Size: 3866
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1885 1885
Size: 3866
FspI TGC'GCA
Cuts at: 1647 1647
Size: 3866
HaeII r_GCGC'y
Cuts at: 1300 1548 2570 3549 1300
Size: 248 1022 979 1617
Fragments arranged by size:
1617 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1708 1738 1740 1770 1708
Size: 30 2 30 3804
Fragments arranged by size:
3804 30 30 2
HincII GTy'rAC
Cuts at: 624 905 624
Size: 281 3585
KasI G'GCGC_C
Cuts at: 1296 1544 1296
Size: 248 3618
KpnI G_GTAC'C
Cuts at: 886 886
Size: 3866
MluI A'CGCG_T
Cuts at: 3299 3299
Size: 3866
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 638 2537 2721 638
Size: 1899 184 1783
Fragments arranged by size:
1899 1783 184
MscI TGG'CCA
Cuts at: 112 1627 112
Size: 1515 2351
MslI CAynn'nnrTG
Cuts at: 274 400 857 1855 1982 274
Size: 126 457 998 127 2158
Fragments arranged by size:
2158 998 457 127 126
MspA1I CmG'CkG
Cuts at: 793 977 1291 1651 2664 2909 793
Size: 184 314 360 1013 245 1750
Fragments arranged by size:
1750 1013 360 314 245 184
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 3866
NarI GG'CG_CC
Cuts at: 1297 1545 1297
Size: 248 3618
NcoI C'CATG_G
Cuts at: 107 107
Size: 3866
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 3866
NspI r_CATG'y
Cuts at: 919 1419 2326 919
Size: 500 907 2459
Fragments arranged by size:
2459 907 500
PciI A'CATG_T
Cuts at: 2322 2322
Size: 3866
PmeI GTTT'AAAC
Cuts at: 865 865
Size: 3866
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 3834
PstI C_TGCA'G
Cuts at: 913 1598 913
Size: 685 3181
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3866
PvuII CAG'CTG
Cuts at: 793 1291 1651 793
Size: 498 360 3008
Fragments arranged by size:
3008 498 360
RsrII CG'GwC_CG
Cuts at: 2061 2061
Size: 3866
SacI G_AGCT'C
Cuts at: 880 880
Size: 3866
SalI G'TCGA_C
Cuts at: 903 903
Size: 3866
SapI GCTCTTCn'nnn_
Cuts at: 1889 2099 1889
Size: 210 3656
SbfI CC_TGCA'GG
Cuts at: 913 913
Size: 3866
ScaI AGT'ACT
Cuts at: 3383 3383
Size: 3866
SfcI C'TryA_G
Cuts at: 33 909 1594 2587 2778 33
Size: 876 685 993 191 1121
Fragments arranged by size:
1121 993 876 685 191
SfoI GGC'GCC
Cuts at: 1298 1546 1298
Size: 248 3618
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3866
SmaI CCC'GGG
Cuts at: 888 888
Size: 3866
SmlI C'TyrA_G
Cuts at: 216 1021 1936 2428 2690 216
Size: 805 915 492 262 1392
Fragments arranged by size:
1392 915 805 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3866
SphI G_CATG'C
Cuts at: 919 1419 919
Size: 500 3366
StyI C'CwwG_G
Cuts at: 107 159 1000 1354 107
Size: 52 841 354 2619
Fragments arranged by size:
2619 841 354 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 288 412 1449 1582 2041 2250 288
Size: 124 1037 133 459 209 1904
Fragments arranged by size:
1904 1037 459 209 133 124
TatI w'GTAC_w
Cuts at: 278 3381 278
Size: 3103 763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 507 539 638 670 3383 3415 507
Size: 32 99 32 2713 32 958
Fragments arranged by size:
2713 958 99 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1663 1663
Size: 3866
XbaI T'CTAG_A
Cuts at: 897 897
Size: 3866
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1361 1361
Size: 3866
XmaI C'CCGG_G
Cuts at: 886 886
Size: 3866
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI