Restriction map for
pF1KE0824
(Circular) (Six-base) MAPSORT of: pF1KE0824.seq Check: 616 from: 1 to: 3917
pF1KE0824 3917 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 955 955
Size: 3917
Acc65I G'GTAC_C
Cuts at: 933 933
Size: 3917
AclI AA'CG_TT
Cuts at: 3757 3757
Size: 3917
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1742 2174 2921 3189 1742
Size: 432 747 268 2470
Fragments arranged by size:
2470 747 432 268
AflIII A'CryG_T
Cuts at: 188 1269 2373 3350 188
Size: 1081 1104 977 755
Fragments arranged by size:
1104 1081 977 755
AgeI A'CCGG_T
Cuts at: 2305 2305
Size: 3917
AleI CACnn'nnGTG
Cuts at: 713 713
Size: 3917
AlwNI CAG_nnn'CTG
Cuts at: 1222 2789 1222
Size: 1567 2350
ApaI G_GGCC'C
Cuts at: 491 491
Size: 3917
ApaLI G'TGCA_C
Cuts at: 399 2687 399
Size: 2288 1629
ApoI r'AATT_y
Cuts at: 518 921 3270 3526 518
Size: 403 2349 256 909
Fragments arranged by size:
2349 909 403 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3917
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
AvaI C'yCGr_G
Cuts at: 491 937 491
Size: 446 3471
BamHI G'GATC_C
Cuts at: 942 942
Size: 3917
BanI G'GyrC_C
Cuts at: 874 933 1347 1595 1630 874
Size: 59 414 248 35 3161
Fragments arranged by size:
3161 414 248 59 35
BanII G_rGCy'C
Cuts at: 491 931 1961 491
Size: 440 1030 2447
Fragments arranged by size:
2447 1030 440
BbeI G_GCGC'C
Cuts at: 878 1351 1599 878
Size: 473 248 3196
Fragments arranged by size:
3196 473 248
BciVI GTATCCnnnnn_n'
Cuts at: 1815 2576 3917 1815
Size: 761 1341 1815
Fragments arranged by size:
1815 1341 761
BclI T'GATC_A
Cuts at: 1437 1437
Size: 3917
BfrBI ATG'CAT
Cuts at: 269 269
Size: 3917
BglII A'GATC_T
Cuts at: 1432 3469 1432
Size: 2037 1880
BlpI GC'TnA_GC
Cuts at: 1029 1029
Size: 3917
Bme1580I G_kGCm'C
Cuts at: 403 491 1542 1635 2691 403
Size: 88 1051 93 1056 1629
Fragments arranged by size:
1629 1056 1051 93 88
BmrI ACTGGGnnnn_n'
Cuts at: 1129 1210 1545 3689 1129
Size: 81 335 2144 1357
Fragments arranged by size:
2144 1357 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 878 2284 878
Size: 1406 2511
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 411 352
Size: 59 3858
Bpu10I CC'TnA_GC
Cuts at: 863 863
Size: 3917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 517 1093 1972 2464 2762 517
Size: 576 879 492 298 1672
Fragments arranged by size:
1672 879 576 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3621 3621
Size: 3917
BsaAI yAC'GTr
Cuts at: 1270 1270
Size: 3917
BsaBI GATnn'nnATC
Cuts at: 6 1431 3468 6
Size: 1425 2037 455
Fragments arranged by size:
2037 1425 455
BsaHI Gr'CG_yC
Cuts at: 875 1348 1596 2298 3794 875
Size: 473 248 702 1496 998
Fragments arranged by size:
1496 998 702 473 248
BsaWI w'CCGG_w
Cuts at: 1106 1627 2305 2579 2726 3137 1106
Size: 521 678 274 147 411 1886
Fragments arranged by size:
1886 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 693 796 693
Size: 103 3814
BseYI C'CCAG_C
Cuts at: 1342 2100 2677 3173 1342
Size: 758 577 496 2086
Fragments arranged by size:
2086 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1459 1505 2713 104
Size: 1355 46 1208 1308
Fragments arranged by size:
1355 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 403 891 931 1709 1899 2691 403
Size: 488 40 778 190 792 1629
Fragments arranged by size:
1629 792 778 488 190 40
BsmI GAATG_Cn'
Cuts at: 864 864
Size: 3917
Bsp1286I G_dGCh'C
Cuts at: 403 491 891 931 1542 1635 1709 1899 1961
Size: 88 400 40 611 93 74 190 62
Cuts at: 1961 2691 403
Size: 730 1629
Fragments arranged by size:
1629 730 611 400 190 93 88 74
62 40
BspEI T'CCGG_A
Cuts at: 3137 3137
Size: 3917
BspHI T'CATG_A
Cuts at: 383 3093 383
Size: 2710 1207
BspMI ACCTGCnnnn'nnnn_
Cuts at: 706 967 1181 1483 1864 706
Size: 261 214 302 381 2759
Fragments arranged by size:
2759 381 302 261 214
BsrBI CCG'CTC
Cuts at: 50 2209 2263 3747 3912 50
Size: 2159 54 1484 165 55
Fragments arranged by size:
2159 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1829 1829
Size: 3917
BsrFI r'CCGG_y
Cuts at: 1915 2096 2305 1915
Size: 181 209 3527
Fragments arranged by size:
3527 209 181
BssSI C'ACGA_G
Cuts at: 2188 2546 2188
Size: 358 3559
BstAPI GCAn_nnn'nTGC
Cuts at: 1294 1294
Size: 3917
BstBI TT'CG_AA
Cuts at: 2278 2278
Size: 3917
BstXI CCAn_nnnn'nTGG
Cuts at: 114 222 114
Size: 108 3809
BstYI r'GATC_y
Cuts at: 1 659 942 1409 1432 1767 2013 3025 3111
Size: 658 283 467 23 335 246 1012 86
Cuts at: 3111 3123 3469 1
Size: 12 346 449
Fragments arranged by size:
1012 658 467 449 346 335 283 246
86 23 12
BtgI C'CryG_G
Cuts at: 743 743
Size: 3917
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1867 2048 2211 1867
Size: 181 163 3573
Fragments arranged by size:
3573 181 163
BtsI GCAGTG_nn'
Cuts at: 889 1160 889
Size: 271 3646
DraI TTT'AAA
Cuts at: 916 916
Size: 3917
DrdI GACnn_nn'nnGTC
Cuts at: 125 1623 2481 125
Size: 1498 858 1561
Fragments arranged by size:
1561 1498 858
EaeI y'GGCC_r
Cuts at: 176 350 1502 1676 2067 2094 3439 176
Size: 174 1152 174 391 27 1345 654
Fragments arranged by size:
1345 1152 654 391 174 174 27
EagI C'GGCC_G
Cuts at: 1502 1502
Size: 3917
EarI CTCTTCn'nnn_
Cuts at: 514 733 1940 2150 514
Size: 219 1207 210 2281
Fragments arranged by size:
2281 1207 219 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2125 2435 2581 3727 2125
Size: 310 146 1146 2315
Fragments arranged by size:
2315 1146 310 146
EcoICRI GAG'CTC
Cuts at: 929 929
Size: 3917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 411 1742 2174 2921 3189 352
Size: 59 1331 432 747 268 1080
Fragments arranged by size:
1331 1080 747 432 268 59
EcoNI CCTnn'n_nnAGG
Cuts at: 339 339
Size: 3917
EcoO109I rG'GnC_Cy
Cuts at: 488 1056 488
Size: 568 3349
EcoRI G'AATT_C
Cuts at: 921 921
Size: 3917
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1936 1936
Size: 3917
FspI TGC'GCA
Cuts at: 1698 1698
Size: 3917
HaeII r_GCGC'y
Cuts at: 878 1351 1599 2621 3600 878
Size: 473 248 1022 979 1195
Fragments arranged by size:
1195 1022 979 473 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 396 428 834 866 1759 1789 1791 1821 396
Size: 32 406 32 893 30 2 30 2492
Fragments arranged by size:
2492 893 406 32 32 30 30 2
HincII GTy'rAC
Cuts at: 561 956 561
Size: 395 3522
HpaI GTT'AAC
Cuts at: 561 561
Size: 3917
KasI G'GCGC_C
Cuts at: 874 1347 1595 874
Size: 473 248 3196
Fragments arranged by size:
3196 473 248
KpnI G_GTAC'C
Cuts at: 937 937
Size: 3917
MluI A'CGCG_T
Cuts at: 3350 3350
Size: 3917
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 227 582 702 2588 2772 227
Size: 355 120 1886 184 1372
Fragments arranged by size:
1886 1372 355 184 120
MscI TGG'CCA
Cuts at: 178 352 1678 178
Size: 174 1326 2417
Fragments arranged by size:
2417 1326 174
MslI CAynn'nnrTG
Cuts at: 187 713 742 1906 2033 187
Size: 526 29 1164 127 2071
Fragments arranged by size:
2071 1164 526 127 29
MspA1I CmG'CkG
Cuts at: 1028 1342 1702 2715 2960 1028
Size: 314 360 1013 245 1985
Fragments arranged by size:
1985 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2098 2098
Size: 3917
NarI GG'CG_CC
Cuts at: 875 1348 1596 875
Size: 473 248 3196
Fragments arranged by size:
3196 473 248
NcoI C'CATG_G
Cuts at: 743 743
Size: 3917
NgoMIV G'CCGG_C
Cuts at: 2096 2096
Size: 3917
NsiI A_TGCA'T
Cuts at: 271 271
Size: 3917
NspI r_CATG'y
Cuts at: 192 457 636 970 1470 2377 192
Size: 265 179 334 500 907 1732
Fragments arranged by size:
1732 907 500 334 265 179
PciI A'CATG_T
Cuts at: 188 2373 188
Size: 2185 1732
PmeI GTTT'AAAC
Cuts at: 916 916
Size: 3917
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 3885
PspOMI G'GGCC_C
Cuts at: 487 487
Size: 3917
PstI C_TGCA'G
Cuts at: 896 964 1649 896
Size: 68 685 3164
Fragments arranged by size:
3164 685 68
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3917
PvuII CAG'CTG
Cuts at: 1342 1702 1342
Size: 360 3557
RsrII CG'GwC_CG
Cuts at: 2112 2112
Size: 3917
SacI G_AGCT'C
Cuts at: 931 931
Size: 3917
SalI G'TCGA_C
Cuts at: 954 954
Size: 3917
SapI GCTCTTCn'nnn_
Cuts at: 1940 2150 1940
Size: 210 3707
SbfI CC_TGCA'GG
Cuts at: 964 964
Size: 3917
ScaI AGT'ACT
Cuts at: 3434 3434
Size: 3917
SfcI C'TryA_G
Cuts at: 33 305 846 892 960 1645 2638 2829 33
Size: 272 541 46 68 685 993 191 1121
Fragments arranged by size:
1121 993 685 541 272 191 68 46
SfoI GGC'GCC
Cuts at: 876 1349 1597 876
Size: 473 248 3196
Fragments arranged by size:
3196 473 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3917
SmaI CCC'GGG
Cuts at: 493 939 493
Size: 446 3471
SmlI C'TyrA_G
Cuts at: 532 1072 1987 2479 2741 532
Size: 540 915 492 262 1708
Fragments arranged by size:
1708 915 540 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3917
SphI G_CATG'C
Cuts at: 970 1470 970
Size: 500 3417
StuI AGG'CCT
Cuts at: 688 688
Size: 3917
StyI C'CwwG_G
Cuts at: 743 1051 1405 743
Size: 308 354 3255
Fragments arranged by size:
3255 354 308
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1500 1633 2092 2301 1500
Size: 133 459 209 3116
Fragments arranged by size:
3116 459 209 133
TatI w'GTAC_w
Cuts at: 155 371 3432 155
Size: 216 3061 640
Fragments arranged by size:
3061 640 216
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 3885
Tth111I GACn'n_nGTC
Cuts at: 903 1714 903
Size: 811 3106
XbaI T'CTAG_A
Cuts at: 948 948
Size: 3917
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1412 1412
Size: 3917
XmaI C'CCGG_G
Cuts at: 491 937 491
Size: 446 3471
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI