Restriction map for
pF1KE0823
(Circular) (Six-base) MAPSORT of: pF1KE0823.seq Check: 1282 from: 1 to: 3812
pF1KE0823 3812 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 790 790
Size: 3812
AccI GT'mk_AC
Cuts at: 850 850
Size: 3812
Acc65I G'GTAC_C
Cuts at: 523 828 523
Size: 305 3507
AclI AA'CG_TT
Cuts at: 3652 3652
Size: 3812
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 1637 2069 2816 3084 682
Size: 955 432 747 268 1410
Fragments arranged by size:
1410 955 747 432 268
AflIII A'CryG_T
Cuts at: 1164 2268 3245 1164
Size: 1104 977 1731
Fragments arranged by size:
1731 1104 977
AgeI A'CCGG_T
Cuts at: 2200 2200
Size: 3812
AleI CACnn'nnGTG
Cuts at: 280 280
Size: 3812
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 511 511
Size: 3812
AlwNI CAG_nnn'CTG
Cuts at: 351 1117 2684 351
Size: 766 1567 1479
Fragments arranged by size:
1567 1479 766
ApaLI G'TGCA_C
Cuts at: 591 2582 591
Size: 1991 1821
ApoI r'AATT_y
Cuts at: 816 3165 3421 816
Size: 2349 256 1207
Fragments arranged by size:
2349 1207 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3812
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
AvaI C'yCGr_G
Cuts at: 832 832
Size: 3812
BamHI G'GATC_C
Cuts at: 837 837
Size: 3812
BanI G'GyrC_C
Cuts at: 523 828 1242 1490 1525 523
Size: 305 414 248 35 2810
Fragments arranged by size:
2810 414 305 248 35
BanII G_rGCy'C
Cuts at: 826 1856 826
Size: 1030 2782
BbeI G_GCGC'C
Cuts at: 1246 1494 1246
Size: 248 3564
BbsI GAAGACnn'nnnn_
Cuts at: 147 147
Size: 3812
BciVI GTATCCnnnnn_n'
Cuts at: 1710 2471 3812 1710
Size: 761 1341 1710
Fragments arranged by size:
1710 1341 761
BclI T'GATC_A
Cuts at: 242 1332 242
Size: 1090 2722
BfrBI ATG'CAT
Cuts at: 722 722
Size: 3812
BglI GCCn_nnn'nGGC
Cuts at: 361 361
Size: 3812
BglII A'GATC_T
Cuts at: 1327 3364 1327
Size: 2037 1775
BlpI GC'TnA_GC
Cuts at: 924 924
Size: 3812
Bme1580I G_kGCm'C
Cuts at: 595 1437 1530 2586 595
Size: 842 93 1056 1821
Fragments arranged by size:
1821 1056 842 93
BmrI ACTGGGnnnn_n'
Cuts at: 1024 1105 1440 3584 1024
Size: 81 335 2144 1252
Fragments arranged by size:
2144 1252 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 3812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 503
Size: 3812
Bpu10I CC'TnA_GC
Cuts at: 295 295
Size: 3812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 988 1867 2359 2657 988
Size: 879 492 298 2143
Fragments arranged by size:
2143 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3516 3516
Size: 3812
BsaAI yAC'GTr
Cuts at: 590 1165 590
Size: 575 3237
BsaBI GATnn'nnATC
Cuts at: 6 1326 3363 6
Size: 1320 2037 455
Fragments arranged by size:
2037 1320 455
BsaHI Gr'CG_yC
Cuts at: 1243 1491 2193 3689 1243
Size: 248 702 1496 1366
Fragments arranged by size:
1496 1366 702 248
BsaWI w'CCGG_w
Cuts at: 1001 1522 2200 2474 2621 3032 1001
Size: 521 678 274 147 411 1781
Fragments arranged by size:
1781 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 501 531 501
Size: 30 3782
BseYI C'CCAG_C
Cuts at: 1237 1995 2572 3068 1237
Size: 758 577 496 1981
Fragments arranged by size:
1981 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 449 481 424
Size: 25 32 3755
Fragments arranged by size:
3755 32 25
BsiEI CG_ry'CG
Cuts at: 104 1354 1400 2608 104
Size: 1250 46 1208 1308
Fragments arranged by size:
1308 1250 1208 46
BsiHKAI G_wGCw'C
Cuts at: 595 826 1604 1794 2586 595
Size: 231 778 190 792 1821
Fragments arranged by size:
1821 792 778 231 190
Bsp1286I G_dGCh'C
Cuts at: 595 826 1437 1530 1604 1794 1856 2586 595
Size: 231 611 93 74 190 62 730 1821
Fragments arranged by size:
1821 730 611 231 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3032 3032
Size: 3812
BspHI T'CATG_A
Cuts at: 2988 2988
Size: 3812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 455 535 790 862 1076 1378 1759 455
Size: 80 255 72 214 302 381 2508
Fragments arranged by size:
2508 381 302 255 214 80 72
BsrBI CCG'CTC
Cuts at: 50 2104 2158 3642 3807 50
Size: 2054 54 1484 165 55
Fragments arranged by size:
2054 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1724 1724
Size: 3812
BsrFI r'CCGG_y
Cuts at: 1810 1991 2200 1810
Size: 181 209 3422
Fragments arranged by size:
3422 209 181
BssSI C'ACGA_G
Cuts at: 2083 2441 2083
Size: 358 3454
BstAPI GCAn_nnn'nTGC
Cuts at: 1189 1189
Size: 3812
BstBI TT'CG_AA
Cuts at: 2173 2173
Size: 3812
BstXI CCAn_nnnn'nTGG
Cuts at: 741 741
Size: 3812
BstYI r'GATC_y
Cuts at: 1 837 1304 1327 1662 1908 2920 3006 3018
Size: 836 467 23 335 246 1012 86 12
Cuts at: 3018 3364 1
Size: 346 449
Fragments arranged by size:
1012 836 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3812
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1762 1943 2106 1762
Size: 181 163 3468
Fragments arranged by size:
3468 181 163
BtsI GCAGTG_nn'
Cuts at: 493 1055 493
Size: 562 3250
DraI TTT'AAA
Cuts at: 811 811
Size: 3812
DraIII CAC_nnn'GTG
Cuts at: 440 440
Size: 3812
DrdI GACnn_nn'nnGTC
Cuts at: 1518 2376 1518
Size: 858 2954
EaeI y'GGCC_r
Cuts at: 110 353 362 542 1397 1571 1962 1989 3334
Size: 243 9 180 855 174 391 27 1345
Cuts at: 3334 110
Size: 588
Fragments arranged by size:
1345 855 588 391 243 180 174 27
9
EagI C'GGCC_G
Cuts at: 1397 1397
Size: 3812
EarI CTCTTCn'nnn_
Cuts at: 193 1835 2045 193
Size: 1642 210 1960
Fragments arranged by size:
1960 1642 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2020 2330 2476 3622 2020
Size: 310 146 1146 2210
Fragments arranged by size:
2210 1146 310 146
EcoICRI GAG'CTC
Cuts at: 824 824
Size: 3812
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 682 1637 2069 2816 3084 503
Size: 179 955 432 747 268 1231
Fragments arranged by size:
1231 955 747 432 268 179
EcoO109I rG'GnC_Cy
Cuts at: 951 951
Size: 3812
EcoRI G'AATT_C
Cuts at: 816 816
Size: 3812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1831 1831
Size: 3812
FspI TGC'GCA
Cuts at: 1593 1593
Size: 3812
HaeII r_GCGC'y
Cuts at: 1246 1494 2516 3495 1246
Size: 248 1022 979 1563
Fragments arranged by size:
1563 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 501 533 1654 1684 1686 1716 501
Size: 32 1121 30 2 30 2597
Fragments arranged by size:
2597 1121 32 30 30 2
HincII GTy'rAC
Cuts at: 851 851
Size: 3812
KasI G'GCGC_C
Cuts at: 1242 1490 1242
Size: 248 3564
KpnI G_GTAC'C
Cuts at: 527 832 527
Size: 305 3507
MluI A'CGCG_T
Cuts at: 3245 3245
Size: 3812
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2483 2667 2483
Size: 184 3628
MscI TGG'CCA
Cuts at: 112 355 364 544 1573 112
Size: 243 9 180 1029 2351
Fragments arranged by size:
2351 1029 243 180 9
MslI CAynn'nnrTG
Cuts at: 280 511 1801 1928 280
Size: 231 1290 127 2164
Fragments arranged by size:
2164 1290 231 127
MspA1I CmG'CkG
Cuts at: 923 1237 1597 2610 2855 923
Size: 314 360 1013 245 1880
Fragments arranged by size:
1880 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1993 1993
Size: 3812
NarI GG'CG_CC
Cuts at: 1243 1491 1243
Size: 248 3564
NcoI C'CATG_G
Cuts at: 107 107
Size: 3812
NdeI CA'TA_TG
Cuts at: 424 424
Size: 3812
NgoMIV G'CCGG_C
Cuts at: 1991 1991
Size: 3812
NsiI A_TGCA'T
Cuts at: 724 724
Size: 3812
NspI r_CATG'y
Cuts at: 726 765 865 1365 2272 726
Size: 39 100 500 907 2266
Fragments arranged by size:
2266 907 500 100 39
PciI A'CATG_T
Cuts at: 2268 2268
Size: 3812
PfoI T'CCnGG_A
Cuts at: 311 311
Size: 3812
PmeI GTTT'AAAC
Cuts at: 811 811
Size: 3812
PmlI CAC'GTG
Cuts at: 590 590
Size: 3812
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2951 2983 2951
Size: 32 3780
PstI C_TGCA'G
Cuts at: 532 676 859 1544 532
Size: 144 183 685 2800
Fragments arranged by size:
2800 685 183 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3812
PvuII CAG'CTG
Cuts at: 1237 1597 1237
Size: 360 3452
RsrII CG'GwC_CG
Cuts at: 2007 2007
Size: 3812
SacI G_AGCT'C
Cuts at: 826 826
Size: 3812
SalI G'TCGA_C
Cuts at: 849 849
Size: 3812
SapI GCTCTTCn'nnn_
Cuts at: 1835 2045 1835
Size: 210 3602
SbfI CC_TGCA'GG
Cuts at: 532 859 532
Size: 327 3485
ScaI AGT'ACT
Cuts at: 3329 3329
Size: 3812
SfcI C'TryA_G
Cuts at: 33 528 672 855 1540 2533 2724 33
Size: 495 144 183 685 993 191 1121
Fragments arranged by size:
1121 993 685 495 191 183 144
SfiI GGCCn_nnn'nGGCC
Cuts at: 361 361
Size: 3812
SfoI GGC'GCC
Cuts at: 1244 1492 1244
Size: 248 3564
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3812
SmaI CCC'GGG
Cuts at: 834 834
Size: 3812
SmlI C'TyrA_G
Cuts at: 967 1882 2374 2636 967
Size: 915 492 262 2143
Fragments arranged by size:
2143 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3812
SphI G_CATG'C
Cuts at: 865 1365 865
Size: 500 3312
StyI C'CwwG_G
Cuts at: 107 946 1300 107
Size: 839 354 2619
Fragments arranged by size:
2619 839 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1395 1528 1987 2196 1395
Size: 133 459 209 3011
Fragments arranged by size:
3011 459 209 133
TatI w'GTAC_w
Cuts at: 284 3327 284
Size: 3043 769
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 3780
Tth111I GACn'n_nGTC
Cuts at: 1609 1609
Size: 3812
XbaI T'CTAG_A
Cuts at: 843 843
Size: 3812
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1307 1307
Size: 3812
XmaI C'CCGG_G
Cuts at: 832 832
Size: 3812
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PfoI PmeI PmlI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PasI PflMI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI