Restriction map for
pF1KE0822
(Circular) (Six-base) MAPSORT of: pF1KE0822.seq Check: 9949 from: 1 to: 3809
pF1KE0822 3809 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AatII G_ACGT'C
Cuts at: 654 654
Size: 3809
AccI GT'mk_AC
Cuts at: 847 847
Size: 3809
Acc65I G'GTAC_C
Cuts at: 825 825
Size: 3809
AclI AA'CG_TT
Cuts at: 3649 3649
Size: 3809
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1634 2066 2813 3081 1634
Size: 432 747 268 2362
Fragments arranged by size:
2362 747 432 268
AflII C'TTAA_G
Cuts at: 348 348
Size: 3809
AflIII A'CryG_T
Cuts at: 440 521 586 1161 2265 3242 440
Size: 81 65 575 1104 977 1007
Fragments arranged by size:
1104 1007 977 575 81 65
AgeI A'CCGG_T
Cuts at: 2197 2197
Size: 3809
AleI CACnn'nnGTG
Cuts at: 706 706
Size: 3809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 219 219
Size: 3809
AlwNI CAG_nnn'CTG
Cuts at: 1114 2681 1114
Size: 1567 2242
ApaLI G'TGCA_C
Cuts at: 2579 2579
Size: 3809
ApoI r'AATT_y
Cuts at: 117 813 3162 3418 117
Size: 696 2349 256 508
Fragments arranged by size:
2349 696 508 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3809
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
AvaI C'yCGr_G
Cuts at: 829 829
Size: 3809
BamHI G'GATC_C
Cuts at: 834 834
Size: 3809
BanI G'GyrC_C
Cuts at: 825 1239 1487 1522 825
Size: 414 248 35 3112
Fragments arranged by size:
3112 414 248 35
BanII G_rGCy'C
Cuts at: 823 1853 823
Size: 1030 2779
BbeI G_GCGC'C
Cuts at: 1243 1491 1243
Size: 248 3561
BbsI GAAGACnn'nnnn_
Cuts at: 142 142
Size: 3809
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3775
BciVI GTATCCnnnnn_n'
Cuts at: 1707 2468 3809 1707
Size: 761 1341 1707
Fragments arranged by size:
1707 1341 761
BclI T'GATC_A
Cuts at: 503 1329 503
Size: 826 2983
BfrBI ATG'CAT
Cuts at: 642 642
Size: 3809
BglII A'GATC_T
Cuts at: 1324 3361 1324
Size: 2037 1772
BlpI GC'TnA_GC
Cuts at: 921 921
Size: 3809
Bme1580I G_kGCm'C
Cuts at: 1434 1527 2583 1434
Size: 93 1056 2660
Fragments arranged by size:
2660 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1021 1102 1437 3581 1021
Size: 81 335 2144 1249
Fragments arranged by size:
2144 1249 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 3809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 985 1864 2356 2654 985
Size: 879 492 298 2140
Fragments arranged by size:
2140 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3513 3513
Size: 3809
BsaAI yAC'GTr
Cuts at: 443 1162 443
Size: 719 3090
BsaBI GATnn'nnATC
Cuts at: 6 1323 3360 6
Size: 1317 2037 455
Fragments arranged by size:
2037 1317 455
BsaHI Gr'CG_yC
Cuts at: 651 1240 1488 2190 3686 651
Size: 589 248 702 1496 774
Fragments arranged by size:
1496 774 702 589 248
BsaWI w'CCGG_w
Cuts at: 998 1519 2197 2471 2618 3029 998
Size: 521 678 274 147 411 1778
Fragments arranged by size:
1778 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 349 379 349
Size: 30 3779
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 404 404
Size: 3809
BseYI C'CCAG_C
Cuts at: 1234 1992 2569 3065 1234
Size: 758 577 496 1978
Fragments arranged by size:
1978 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1351 1397 2605 104
Size: 1247 46 1208 1308
Fragments arranged by size:
1308 1247 1208 46
BsiHKAI G_wGCw'C
Cuts at: 203 823 1601 1791 2583 203
Size: 620 778 190 792 1429
Fragments arranged by size:
1429 792 778 620 190
BsmBI CGTCTCn'nnnn_
Cuts at: 642 642
Size: 3809
Bsp1286I G_dGCh'C
Cuts at: 203 823 1434 1527 1601 1791 1853 2583 203
Size: 620 611 93 74 190 62 730 1429
Fragments arranged by size:
1429 730 620 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3029 3029
Size: 3809
BspHI T'CATG_A
Cuts at: 2985 2985
Size: 3809
BspMI ACCTGCnnnn'nnnn_
Cuts at: 142 859 1073 1375 1756 142
Size: 717 214 302 381 2195
Fragments arranged by size:
2195 717 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2101 2155 3639 3804 50
Size: 2051 54 1484 165 55
Fragments arranged by size:
2051 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 179 253 1721 179
Size: 74 1468 2267
Fragments arranged by size:
2267 1468 74
BsrFI r'CCGG_y
Cuts at: 475 1807 1988 2197 475
Size: 1332 181 209 2087
Fragments arranged by size:
2087 1332 209 181
BsrGI T'GTAC_A
Cuts at: 464 464
Size: 3809
BssSI C'ACGA_G
Cuts at: 2080 2438 2080
Size: 358 3451
BstAPI GCAn_nnn'nTGC
Cuts at: 1186 1186
Size: 3809
BstBI TT'CG_AA
Cuts at: 2170 2170
Size: 3809
BstYI r'GATC_y
Cuts at: 1 834 1301 1324 1659 1905 2917 3003 3015
Size: 833 467 23 335 246 1012 86 12
Cuts at: 3015 3361 1
Size: 346 449
Fragments arranged by size:
1012 833 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 742 742
Size: 3809
BtgI C'CryG_G
Cuts at: 668 668
Size: 3809
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1759 1940 2103 1759
Size: 181 163 3465
Fragments arranged by size:
3465 181 163
BtsI GCAGTG_nn'
Cuts at: 172 201 1052 172
Size: 29 851 2929
Fragments arranged by size:
2929 851 29
DraI TTT'AAA
Cuts at: 808 808
Size: 3809
DrdI GACnn_nn'nnGTC
Cuts at: 1515 2373 1515
Size: 858 2951
EaeI y'GGCC_r
Cuts at: 239 1394 1568 1959 1986 3331 239
Size: 1155 174 391 27 1345 717
Fragments arranged by size:
1345 1155 717 391 174 27
EagI C'GGCC_G
Cuts at: 1394 1394
Size: 3809
EarI CTCTTCn'nnn_
Cuts at: 503 637 1832 2042 503
Size: 134 1195 210 2270
Fragments arranged by size:
2270 1195 210 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2017 2327 2473 3619 2017
Size: 310 146 1146 2207
Fragments arranged by size:
2207 1146 310 146
EcoICRI GAG'CTC
Cuts at: 821 821
Size: 3809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1634 2066 2813 3081 1634
Size: 432 747 268 2362
Fragments arranged by size:
2362 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 353 948 353
Size: 595 3214
EcoRI G'AATT_C
Cuts at: 813 813
Size: 3809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1828 1828
Size: 3809
FspI TGC'GCA
Cuts at: 1590 1590
Size: 3809
HaeII r_GCGC'y
Cuts at: 1243 1491 2513 3492 1243
Size: 248 1022 979 1560
Fragments arranged by size:
1560 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1651 1681 1683 1713 1651
Size: 30 2 30 3747
Fragments arranged by size:
3747 30 30 2
HincII GTy'rAC
Cuts at: 848 848
Size: 3809
HindIII A'AGCT_T
Cuts at: 575 575
Size: 3809
KasI G'GCGC_C
Cuts at: 1239 1487 1239
Size: 248 3561
KpnI G_GTAC'C
Cuts at: 829 829
Size: 3809
MluI A'CGCG_T
Cuts at: 3242 3242
Size: 3809
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2480 2664 2480
Size: 184 3625
MscI TGG'CCA
Cuts at: 241 1570 241
Size: 1329 2480
MslI CAynn'nnrTG
Cuts at: 184 706 1798 1925 184
Size: 522 1092 127 2068
Fragments arranged by size:
2068 1092 522 127
MspA1I CmG'CkG
Cuts at: 920 1234 1594 2607 2852 920
Size: 314 360 1013 245 1877
Fragments arranged by size:
1877 1013 360 314 245
NaeI GCC'GGC
Cuts at: 477 1990 477
Size: 1513 2296
NarI GG'CG_CC
Cuts at: 1240 1488 1240
Size: 248 3561
NdeI CA'TA_TG
Cuts at: 225 225
Size: 3809
NgoMIV G'CCGG_C
Cuts at: 475 1988 475
Size: 1513 2296
NsiI A_TGCA'T
Cuts at: 644 644
Size: 3809
NspI r_CATG'y
Cuts at: 210 434 525 590 642 862 1362 2269 210
Size: 224 91 65 52 220 500 907 1750
Fragments arranged by size:
1750 907 500 224 220 91 65 52
PciI A'CATG_T
Cuts at: 521 586 2265 521
Size: 65 1679 2065
Fragments arranged by size:
2065 1679 65
PmeI GTTT'AAAC
Cuts at: 808 808
Size: 3809
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2948 2980 2948
Size: 32 3777
PpuMI rG'GwC_Cy
Cuts at: 353 353
Size: 3809
PshAI GACnn'nnGTC
Cuts at: 607 607
Size: 3809
PstI C_TGCA'G
Cuts at: 561 856 1541 561
Size: 295 685 2829
Fragments arranged by size:
2829 685 295
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3809
PvuII CAG'CTG
Cuts at: 1234 1594 1234
Size: 360 3449
RsrII CG'GwC_CG
Cuts at: 2004 2004
Size: 3809
SacI G_AGCT'C
Cuts at: 823 823
Size: 3809
SalI G'TCGA_C
Cuts at: 846 846
Size: 3809
SapI GCTCTTCn'nnn_
Cuts at: 1832 2042 1832
Size: 210 3599
SbfI CC_TGCA'GG
Cuts at: 856 856
Size: 3809
ScaI AGT'ACT
Cuts at: 3326 3326
Size: 3809
SexAI A'CCwGG_T
Cuts at: 296 296
Size: 3809
SfcI C'TryA_G
Cuts at: 33 261 480 557 852 1537 2530 2721 33
Size: 228 219 77 295 685 993 191 1121
Fragments arranged by size:
1121 993 685 295 228 219 191 77
SfoI GGC'GCC
Cuts at: 1241 1489 1241
Size: 248 3561
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3809
SmaI CCC'GGG
Cuts at: 831 831
Size: 3809
SmlI C'TyrA_G
Cuts at: 348 964 1879 2371 2633 348
Size: 616 915 492 262 1524
Fragments arranged by size:
1524 915 616 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3809
SphI G_CATG'C
Cuts at: 434 642 862 1362 434
Size: 208 220 500 2881
Fragments arranged by size:
2881 500 220 208
StyI C'CwwG_G
Cuts at: 279 943 1297 279
Size: 664 354 2791
Fragments arranged by size:
2791 664 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1392 1525 1984 2193 1392
Size: 133 459 209 3008
Fragments arranged by size:
3008 459 209 133
TatI w'GTAC_w
Cuts at: 464 3324 464
Size: 2860 949
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3326 3358 3326
Size: 32 3777
Tth111I GACn'n_nGTC
Cuts at: 1606 1606
Size: 3809
XbaI T'CTAG_A
Cuts at: 840 840
Size: 3809
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1304 1304
Size: 3809
XmaI C'CCGG_G
Cuts at: 829 829
Size: 3809
XmnI GAAnn'nnTTC
Cuts at: 552 552
Size: 3809
ZraI GAC'GTC
Cuts at: 652 652
Size: 3809
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NsiI NspI PciI
PmeI PpiI PpuMI PshAI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AloI ApaI AscI AvrII BaeI
BbvCI BglI BmgBI BmtI BpmI Bpu10I BsgI BsiWI
BsmI BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NheI
NotI NruI PacI PasI PflMI PfoI PmlI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI