Restriction map for
pF1KE0820
(Circular) (Six-base) MAPSORT of: pF1KE0820.seq Check: 6412 from: 1 to: 3695
pF1KE0820 3695 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AatII G_ACGT'C
Cuts at: 275 275
Size: 3695
AccI GT'mk_AC
Cuts at: 733 733
Size: 3695
Acc65I G'GTAC_C
Cuts at: 711 711
Size: 3695
AclI AA'CG_TT
Cuts at: 3535 3535
Size: 3695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1952 2699 2967 1520
Size: 432 747 268 2248
Fragments arranged by size:
2248 747 432 268
AflIII A'CryG_T
Cuts at: 1047 2151 3128 1047
Size: 1104 977 1614
Fragments arranged by size:
1614 1104 977
AgeI A'CCGG_T
Cuts at: 2083 2083
Size: 3695
AlwNI CAG_nnn'CTG
Cuts at: 1000 2567 1000
Size: 1567 2128
ApaLI G'TGCA_C
Cuts at: 2465 2465
Size: 3695
ApoI r'AATT_y
Cuts at: 555 699 3048 3304 555
Size: 144 2349 256 946
Fragments arranged by size:
2349 946 256 144
AseI AT'TA_AT
Cuts at: 20 20
Size: 3695
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
AvaI C'yCGr_G
Cuts at: 715 715
Size: 3695
BamHI G'GATC_C
Cuts at: 720 720
Size: 3695
BanI G'GyrC_C
Cuts at: 711 1125 1373 1408 711
Size: 414 248 35 2998
Fragments arranged by size:
2998 414 248 35
BanII G_rGCy'C
Cuts at: 709 1739 709
Size: 1030 2665
BbeI G_GCGC'C
Cuts at: 1129 1377 1129
Size: 248 3447
BbsI GAAGACnn'nnnn_
Cuts at: 437 437
Size: 3695
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3661
BciVI GTATCCnnnnn_n'
Cuts at: 1593 2354 3695 1593
Size: 761 1341 1593
Fragments arranged by size:
1593 1341 761
BclI T'GATC_A
Cuts at: 1215 1215
Size: 3695
BglII A'GATC_T
Cuts at: 1210 3247 1210
Size: 2037 1658
BlpI GC'TnA_GC
Cuts at: 807 807
Size: 3695
Bme1580I G_kGCm'C
Cuts at: 1320 1413 2469 1320
Size: 93 1056 2546
Fragments arranged by size:
2546 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 389 907 988 1323 3467 389
Size: 518 81 335 2144 617
Fragments arranged by size:
2144 617 518 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2062 2062
Size: 3695
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 871 1750 2242 2540 871
Size: 879 492 298 2026
Fragments arranged by size:
2026 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3399 3399
Size: 3695
BsaAI yAC'GTr
Cuts at: 126 1048 126
Size: 922 2773
BsaBI GATnn'nnATC
Cuts at: 6 1209 3246 6
Size: 1203 2037 455
Fragments arranged by size:
2037 1203 455
BsaHI Gr'CG_yC
Cuts at: 272 1126 1374 2076 3572 272
Size: 854 248 702 1496 395
Fragments arranged by size:
1496 854 702 395 248
BsaWI w'CCGG_w
Cuts at: 884 1405 2083 2357 2504 2915 884
Size: 521 678 274 147 411 1664
Fragments arranged by size:
1664 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1120 1878 2455 2951 1120
Size: 758 577 496 1864
Fragments arranged by size:
1864 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 480 480
Size: 3695
BsiEI CG_ry'CG
Cuts at: 104 120 1237 1283 2491 104
Size: 16 1117 46 1208 1308
Fragments arranged by size:
1308 1208 1117 46 16
BsiHKAI G_wGCw'C
Cuts at: 709 1487 1677 2469 709
Size: 778 190 792 1935
Fragments arranged by size:
1935 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 709 1320 1413 1487 1677 1739 2469 709
Size: 611 93 74 190 62 730 1935
Fragments arranged by size:
1935 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2915 2915
Size: 3695
BspHI T'CATG_A
Cuts at: 353 2871 353
Size: 2518 1177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 542 745 959 1261 1642 542
Size: 203 214 302 381 2595
Fragments arranged by size:
2595 381 302 214 203
BsrBI CCG'CTC
Cuts at: 50 1987 2041 3525 3690 50
Size: 1937 54 1484 165 55
Fragments arranged by size:
1937 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1607 1607
Size: 3695
BsrFI r'CCGG_y
Cuts at: 1693 1874 2083 1693
Size: 181 209 3305
Fragments arranged by size:
3305 209 181
BssSI C'ACGA_G
Cuts at: 1966 2324 1966
Size: 358 3337
BstAPI GCAn_nnn'nTGC
Cuts at: 1072 1072
Size: 3695
BstBI TT'CG_AA
Cuts at: 2056 2056
Size: 3695
BstYI r'GATC_y
Cuts at: 1 720 1187 1210 1545 1791 2803 2889 2901
Size: 719 467 23 335 246 1012 86 12
Cuts at: 2901 3247 1
Size: 346 449
Fragments arranged by size:
1012 719 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1645 1826 1989 1645
Size: 181 163 3351
Fragments arranged by size:
3351 181 163
BtsI GCAGTG_nn'
Cuts at: 938 938
Size: 3695
DraI TTT'AAA
Cuts at: 694 694
Size: 3695
DrdI GACnn_nn'nnGTC
Cuts at: 1401 2259 1401
Size: 858 2837
EaeI y'GGCC_r
Cuts at: 128 1280 1454 1845 1872 3217 128
Size: 1152 174 391 27 1345 606
Fragments arranged by size:
1345 1152 606 391 174 27
EagI C'GGCC_G
Cuts at: 1280 1280
Size: 3695
EarI CTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1903 2213 2359 3505 1903
Size: 310 146 1146 2093
Fragments arranged by size:
2093 1146 310 146
EcoICRI GAG'CTC
Cuts at: 707 707
Size: 3695
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1520 1952 2699 2967 1520
Size: 432 747 268 2248
Fragments arranged by size:
2248 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 383 834 383
Size: 451 3244
EcoRI G'AATT_C
Cuts at: 555 699 555
Size: 144 3551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1714 1714
Size: 3695
FspI TGC'GCA
Cuts at: 1476 1476
Size: 3695
HaeII r_GCGC'y
Cuts at: 1129 1377 2399 3378 1129
Size: 248 1022 979 1446
Fragments arranged by size:
1446 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1537 1567 1569 1599 1537
Size: 30 2 30 3633
Fragments arranged by size:
3633 30 30 2
HincII GTy'rAC
Cuts at: 734 734
Size: 3695
KasI G'GCGC_C
Cuts at: 1125 1373 1125
Size: 248 3447
KpnI G_GTAC'C
Cuts at: 715 715
Size: 3695
MluI A'CGCG_T
Cuts at: 3128 3128
Size: 3695
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 293 2366 2550 293
Size: 2073 184 1438
Fragments arranged by size:
2073 1438 184
MscI TGG'CCA
Cuts at: 130 1456 130
Size: 1326 2369
MslI CAynn'nnrTG
Cuts at: 1684 1811 1684
Size: 127 3568
MspA1I CmG'CkG
Cuts at: 171 252 806 1120 1480 2493 2738 171
Size: 81 554 314 360 1013 245 1128
Fragments arranged by size:
1128 1013 554 360 314 245 81
NaeI GCC'GGC
Cuts at: 1876 1876
Size: 3695
NarI GG'CG_CC
Cuts at: 1126 1374 1126
Size: 248 3447
NgoMIV G'CCGG_C
Cuts at: 1874 1874
Size: 3695
NspI r_CATG'y
Cuts at: 748 1248 2155 748
Size: 500 907 2288
Fragments arranged by size:
2288 907 500
PciI A'CATG_T
Cuts at: 2151 2151
Size: 3695
PmeI GTTT'AAAC
Cuts at: 694 694
Size: 3695
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2834 2866 2834
Size: 32 3663
PpuMI rG'GwC_Cy
Cuts at: 383 383
Size: 3695
PsiI TTA'TAA
Cuts at: 433 433
Size: 3695
PstI C_TGCA'G
Cuts at: 181 369 539 742 1427 181
Size: 188 170 203 685 2449
Fragments arranged by size:
2449 685 203 188 170
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3695
PvuII CAG'CTG
Cuts at: 252 1120 1480 252
Size: 868 360 2467
Fragments arranged by size:
2467 868 360
RsrII CG'GwC_CG
Cuts at: 1890 1890
Size: 3695
SacI G_AGCT'C
Cuts at: 709 709
Size: 3695
SalI G'TCGA_C
Cuts at: 732 732
Size: 3695
SanDI GG'GwC_CC
Cuts at: 383 383
Size: 3695
SapI GCTCTTCn'nnn_
Cuts at: 1718 1928 1718
Size: 210 3485
SbfI CC_TGCA'GG
Cuts at: 742 742
Size: 3695
ScaI AGT'ACT
Cuts at: 3212 3212
Size: 3695
SfcI C'TryA_G
Cuts at: 33 177 365 535 738 1423 2416 2607 33
Size: 144 188 170 203 685 993 191 1121
Fragments arranged by size:
1121 993 685 203 191 188 170 144
SfoI GGC'GCC
Cuts at: 1127 1375 1127
Size: 248 3447
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3695
SmaI CCC'GGG
Cuts at: 717 717
Size: 3695
SmlI C'TyrA_G
Cuts at: 850 1765 2257 2519 850
Size: 915 492 262 2026
Fragments arranged by size:
2026 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3695
SphI G_CATG'C
Cuts at: 748 1248 748
Size: 500 3195
StyI C'CwwG_G
Cuts at: 829 1183 829
Size: 354 3341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 346 1278 1411 1870 2079 346
Size: 932 133 459 209 1962
Fragments arranged by size:
1962 932 459 209 133
TatI w'GTAC_w
Cuts at: 3210 3210
Size: 3695
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 3663
Tth111I GACn'n_nGTC
Cuts at: 1492 1492
Size: 3695
XbaI T'CTAG_A
Cuts at: 726 726
Size: 3695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1190 1190
Size: 3695
XmaI C'CCGG_G
Cuts at: 715 715
Size: 3695
ZraI GAC'GTC
Cuts at: 273 273
Size: 3695
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
BpmI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI