Restriction map for
pF1KE0817
(Circular) (Six-base) MAPSORT of: pF1KE0817.seq Check: 7860 from: 1 to: 3875
pF1KE0817 3875 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AatII G_ACGT'C
Cuts at: 329 329
Size: 3875
AccI GT'mk_AC
Cuts at: 913 913
Size: 3875
Acc65I G'GTAC_C
Cuts at: 891 891
Size: 3875
AclI AA'CG_TT
Cuts at: 3715 3715
Size: 3875
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 468 1700 2132 2879 3147 150
Size: 318 1232 432 747 268 878
Fragments arranged by size:
1232 878 747 432 318 268
AflIII A'CryG_T
Cuts at: 521 1227 2331 3308 521
Size: 706 1104 977 1088
Fragments arranged by size:
1104 1088 977 706
AgeI A'CCGG_T
Cuts at: 2263 2263
Size: 3875
AlwNI CAG_nnn'CTG
Cuts at: 1180 2747 1180
Size: 1567 2308
ApaLI G'TGCA_C
Cuts at: 2645 2645
Size: 3875
ApoI r'AATT_y
Cuts at: 879 3228 3484 879
Size: 2349 256 1270
Fragments arranged by size:
2349 1270 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3875
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
AvaI C'yCGr_G
Cuts at: 895 895
Size: 3875
BamHI G'GATC_C
Cuts at: 900 900
Size: 3875
BanI G'GyrC_C
Cuts at: 891 1305 1553 1588 891
Size: 414 248 35 3178
Fragments arranged by size:
3178 414 248 35
BanII G_rGCy'C
Cuts at: 889 1919 889
Size: 1030 2845
BbeI G_GCGC'C
Cuts at: 1309 1557 1309
Size: 248 3627
BciVI GTATCCnnnnn_n'
Cuts at: 792 1773 2534 3875 792
Size: 981 761 1341 792
Fragments arranged by size:
1341 981 792 761
BclI T'GATC_A
Cuts at: 1395 1395
Size: 3875
BglII A'GATC_T
Cuts at: 1390 3427 1390
Size: 2037 1838
BlpI GC'TnA_GC
Cuts at: 987 987
Size: 3875
Bme1580I G_kGCm'C
Cuts at: 1500 1593 2649 1500
Size: 93 1056 2726
Fragments arranged by size:
2726 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 408 1087 1168 1503 3647 408
Size: 679 81 335 2144 636
Fragments arranged by size:
2144 679 636 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2242 2242
Size: 3875
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 789 789
Size: 3875
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1051 1930 2422 2720 1051
Size: 879 492 298 2206
Fragments arranged by size:
2206 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3579 3579
Size: 3875
BsaAI yAC'GTr
Cuts at: 1228 1228
Size: 3875
BsaBI GATnn'nnATC
Cuts at: 6 256 659 1389 3426 6
Size: 250 403 730 2037 455
Fragments arranged by size:
2037 730 455 403 250
BsaHI Gr'CG_yC
Cuts at: 326 531 1306 1554 2256 3752 326
Size: 205 775 248 702 1496 449
Fragments arranged by size:
1496 775 702 449 248 205
BsaWI w'CCGG_w
Cuts at: 1064 1585 2263 2537 2684 3095 1064
Size: 521 678 274 147 411 1844
Fragments arranged by size:
1844 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 184 184
Size: 3875
BseYI C'CCAG_C
Cuts at: 275 572 1300 2058 2635 3131 275
Size: 297 728 758 577 496 1019
Fragments arranged by size:
1019 758 728 577 496 297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 522 522
Size: 3875
BsiEI CG_ry'CG
Cuts at: 104 1417 1463 2671 104
Size: 1313 46 1208 1308
Fragments arranged by size:
1313 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 889 1667 1857 2649 889
Size: 778 190 792 2115
Fragments arranged by size:
2115 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 333 333
Size: 3875
Bsp1286I G_dGCh'C
Cuts at: 889 1500 1593 1667 1857 1919 2649 889
Size: 611 93 74 190 62 730 2115
Fragments arranged by size:
2115 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3095 3095
Size: 3875
BspHI T'CATG_A
Cuts at: 3051 3051
Size: 3875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 391 925 1139 1441 1822 391
Size: 534 214 302 381 2444
Fragments arranged by size:
2444 534 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2167 2221 3705 3870 50
Size: 2117 54 1484 165 55
Fragments arranged by size:
2117 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 635 1787 635
Size: 1152 2723
BsrFI r'CCGG_y
Cuts at: 1873 2054 2263 1873
Size: 181 209 3485
Fragments arranged by size:
3485 209 181
BssSI C'ACGA_G
Cuts at: 2146 2504 2146
Size: 358 3517
BstAPI GCAn_nnn'nTGC
Cuts at: 1252 1252
Size: 3875
BstBI TT'CG_AA
Cuts at: 2236 2236
Size: 3875
BstYI r'GATC_y
Cuts at: 1 797 900 1367 1390 1725 1971 2983 3069
Size: 796 103 467 23 335 246 1012 86
Cuts at: 3069 3081 3427 1
Size: 12 346 449
Fragments arranged by size:
1012 796 467 449 346 335 246 103
86 23 12
BtgI C'CryG_G
Cuts at: 107 359 467 107
Size: 252 108 3515
Fragments arranged by size:
3515 252 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1825 2006 2169 1825
Size: 181 163 3531
Fragments arranged by size:
3531 181 163
BtsI GCAGTG_nn'
Cuts at: 1118 1118
Size: 3875
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 327 362 327
Size: 35 3840
DraI TTT'AAA
Cuts at: 874 874
Size: 3875
DrdI GACnn_nn'nnGTC
Cuts at: 1581 2439 1581
Size: 858 3017
EaeI y'GGCC_r
Cuts at: 1460 1634 2025 2052 3397 1460
Size: 174 391 27 1345 1938
Fragments arranged by size:
1938 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1460 1460
Size: 3875
EarI CTCTTCn'nnn_
Cuts at: 202 509 1898 2108 202
Size: 307 1389 210 1969
Fragments arranged by size:
1969 1389 307 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 163 2083 2393 2539 3685 163
Size: 1920 310 146 1146 353
Fragments arranged by size:
1920 1146 353 310 146
EcoICRI GAG'CTC
Cuts at: 887 887
Size: 3875
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 150 468 789 1700 2132 2879 3147 150
Size: 318 321 911 432 747 268 878
Fragments arranged by size:
911 878 747 432 321 318 268
EcoO109I rG'GnC_Cy
Cuts at: 1014 1014
Size: 3875
EcoRI G'AATT_C
Cuts at: 879 879
Size: 3875
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 843 1894 843
Size: 1051 2824
FspI TGC'GCA
Cuts at: 1656 1656
Size: 3875
HaeII r_GCGC'y
Cuts at: 1309 1557 2579 3558 1309
Size: 248 1022 979 1626
Fragments arranged by size:
1626 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 696 728 1717 1747 1749 1779 696
Size: 32 989 30 2 30 2792
Fragments arranged by size:
2792 989 32 30 30 2
HincII GTy'rAC
Cuts at: 914 914
Size: 3875
KasI G'GCGC_C
Cuts at: 1305 1553 1305
Size: 248 3627
KpnI G_GTAC'C
Cuts at: 895 895
Size: 3875
MfeI C'AATT_G
Cuts at: 746 746
Size: 3875
MluI A'CGCG_T
Cuts at: 3308 3308
Size: 3875
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2546 2730 2546
Size: 184 3691
MscI TGG'CCA
Cuts at: 1636 1636
Size: 3875
MslI CAynn'nnrTG
Cuts at: 137 247 289 851 1864 1991 137
Size: 110 42 562 1013 127 2021
Fragments arranged by size:
2021 1013 562 127 110 42
MspA1I CmG'CkG
Cuts at: 279 986 1300 1660 2673 2918 279
Size: 707 314 360 1013 245 1236
Fragments arranged by size:
1236 1013 707 360 314 245
NaeI GCC'GGC
Cuts at: 2056 2056
Size: 3875
NarI GG'CG_CC
Cuts at: 1306 1554 1306
Size: 248 3627
NcoI C'CATG_G
Cuts at: 107 359 467 107
Size: 252 108 3515
Fragments arranged by size:
3515 252 108
NgoMIV G'CCGG_C
Cuts at: 2054 2054
Size: 3875
NspI r_CATG'y
Cuts at: 525 928 1428 2335 525
Size: 403 500 907 2065
Fragments arranged by size:
2065 907 500 403
PciI A'CATG_T
Cuts at: 521 2331 521
Size: 1810 2065
PmeI GTTT'AAAC
Cuts at: 874 874
Size: 3875
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 3014
Size: 32 3843
PstI C_TGCA'G
Cuts at: 922 1607 922
Size: 685 3190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3875
PvuII CAG'CTG
Cuts at: 279 1300 1660 279
Size: 1021 360 2494
Fragments arranged by size:
2494 1021 360
RsrII CG'GwC_CG
Cuts at: 2070 2070
Size: 3875
SacI G_AGCT'C
Cuts at: 889 889
Size: 3875
SalI G'TCGA_C
Cuts at: 912 912
Size: 3875
SapI GCTCTTCn'nnn_
Cuts at: 1898 2108 1898
Size: 210 3665
SbfI CC_TGCA'GG
Cuts at: 922 922
Size: 3875
ScaI AGT'ACT
Cuts at: 3392 3392
Size: 3875
SfcI C'TryA_G
Cuts at: 33 219 918 1603 2596 2787 33
Size: 186 699 685 993 191 1121
Fragments arranged by size:
1121 993 699 685 191 186
SfoI GGC'GCC
Cuts at: 1307 1555 1307
Size: 248 3627
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3875
SmaI CCC'GGG
Cuts at: 897 897
Size: 3875
SmlI C'TyrA_G
Cuts at: 1030 1945 2437 2699 1030
Size: 915 492 262 2206
Fragments arranged by size:
2206 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3875
SphI G_CATG'C
Cuts at: 928 1428 928
Size: 500 3375
StyI C'CwwG_G
Cuts at: 107 359 467 1009 1363 107
Size: 252 108 542 354 2619
Fragments arranged by size:
2619 542 354 252 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 100 841 1458 1591 2050 2259 100
Size: 741 617 133 459 209 1716
Fragments arranged by size:
1716 741 617 459 209 133
TatI w'GTAC_w
Cuts at: 3390 3390
Size: 3875
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 231 263 3392 3424 231
Size: 32 3129 32 682
Fragments arranged by size:
3129 682 32 32
Tth111I GACn'n_nGTC
Cuts at: 1672 1672
Size: 3875
XbaI T'CTAG_A
Cuts at: 906 906
Size: 3875
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1370 1370
Size: 3875
XmaI C'CCGG_G
Cuts at: 895 895
Size: 3875
ZraI GAC'GTC
Cuts at: 327 327
Size: 3875
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BsiWI BsmI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI