Restriction map for
pF1KE0816
(Circular) (Six-base) MAPSORT of: pF1KE0816.seq Check: 9932 from: 1 to: 3791
pF1KE0816 3791 bp
With 182 enzymes: SgfI *
April 24, 2009 13:18 ..
AccI GT'mk_AC
Cuts at: 209 650 829 209
Size: 441 179 3171
Fragments arranged by size:
3171 441 179
Acc65I G'GTAC_C
Cuts at: 473 807 473
Size: 334 3457
AclI AA'CG_TT
Cuts at: 3631 3631
Size: 3791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
AflIII A'CryG_T
Cuts at: 1143 2247 3224 1143
Size: 1104 977 1710
Fragments arranged by size:
1710 1104 977
AgeI A'CCGG_T
Cuts at: 2179 2179
Size: 3791
AleI CACnn'nnGTG
Cuts at: 739 739
Size: 3791
AlwNI CAG_nnn'CTG
Cuts at: 1096 2663 1096
Size: 1567 2224
ApaLI G'TGCA_C
Cuts at: 2561 2561
Size: 3791
ApoI r'AATT_y
Cuts at: 173 357 795 3144 3400 173
Size: 184 438 2349 256 564
Fragments arranged by size:
2349 564 438 256 184
AseI AT'TA_AT
Cuts at: 20 20
Size: 3791
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
AvaI C'yCGr_G
Cuts at: 811 811
Size: 3791
BamHI G'GATC_C
Cuts at: 816 816
Size: 3791
BanI G'GyrC_C
Cuts at: 473 807 1221 1469 1504 473
Size: 334 414 248 35 2760
Fragments arranged by size:
2760 414 334 248 35
BanII G_rGCy'C
Cuts at: 718 805 1835 718
Size: 87 1030 2674
Fragments arranged by size:
2674 1030 87
BbeI G_GCGC'C
Cuts at: 1225 1473 1225
Size: 248 3543
BciVI GTATCCnnnnn_n'
Cuts at: 1689 2450 3791 1689
Size: 761 1341 1689
Fragments arranged by size:
1689 1341 761
BclI T'GATC_A
Cuts at: 630 1311 630
Size: 681 3110
BglII A'GATC_T
Cuts at: 317 1306 3343 317
Size: 989 2037 765
Fragments arranged by size:
2037 989 765
BlpI GC'TnA_GC
Cuts at: 903 903
Size: 3791
Bme1580I G_kGCm'C
Cuts at: 433 1416 1509 2565 433
Size: 983 93 1056 1659
Fragments arranged by size:
1659 1056 983 93
BmrI ACTGGGnnnn_n'
Cuts at: 236 1003 1084 1419 3563 236
Size: 767 81 335 2144 464
Fragments arranged by size:
2144 767 464 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2158 2158
Size: 3791
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 967 1846 2338 2636 967
Size: 879 492 298 2122
Fragments arranged by size:
2122 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3495 3495
Size: 3791
BsaAI yAC'GTr
Cuts at: 288 1144 288
Size: 856 2935
BsaBI GATnn'nnATC
Cuts at: 6 1305 3342 6
Size: 1299 2037 455
Fragments arranged by size:
2037 1299 455
BsaHI Gr'CG_yC
Cuts at: 1222 1470 2172 3668 1222
Size: 248 702 1496 1345
Fragments arranged by size:
1496 1345 702 248
BsaWI w'CCGG_w
Cuts at: 980 1501 2179 2453 2600 3011 980
Size: 521 678 274 147 411 1760
Fragments arranged by size:
1760 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 490 520 490
Size: 30 3761
BseYI C'CCAG_C
Cuts at: 1216 1974 2551 3047 1216
Size: 758 577 496 1960
Fragments arranged by size:
1960 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 269 318 481 269
Size: 49 163 3579
Fragments arranged by size:
3579 163 49
BsiEI CG_ry'CG
Cuts at: 104 1333 1379 2587 104
Size: 1229 46 1208 1308
Fragments arranged by size:
1308 1229 1208 46
BsiHKAI G_wGCw'C
Cuts at: 293 718 773 805 1583 1773 2565 293
Size: 425 55 32 778 190 792 1519
Fragments arranged by size:
1519 792 778 425 190 55 32
BsmI GAATG_Cn'
Cuts at: 445 445
Size: 3791
Bsp1286I G_dGCh'C
Cuts at: 293 433 718 773 805 1416 1509 1583 1773
Size: 140 285 55 32 611 93 74 190
Cuts at: 1773 1835 2565 293
Size: 62 730 1519
Fragments arranged by size:
1519 730 611 285 190 140 93 74
62 55 32
BspEI T'CCGG_A
Cuts at: 3011 3011
Size: 3791
BspHI T'CATG_A
Cuts at: 2967 2967
Size: 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 340 841 1055 1357 1738 340
Size: 501 214 302 381 2393
Fragments arranged by size:
2393 501 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2083 2137 3621 3786 50
Size: 2033 54 1484 165 55
Fragments arranged by size:
2033 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1703 1703
Size: 3791
BsrFI r'CCGG_y
Cuts at: 1789 1970 2179 1789
Size: 181 209 3401
Fragments arranged by size:
3401 209 181
BsrGI T'GTAC_A
Cuts at: 743 743
Size: 3791
BssSI C'ACGA_G
Cuts at: 2062 2420 2062
Size: 358 3433
BstAPI GCAn_nnn'nTGC
Cuts at: 1168 1168
Size: 3791
BstBI TT'CG_AA
Cuts at: 2152 2152
Size: 3791
BstXI CCAn_nnnn'nTGG
Cuts at: 351 529 351
Size: 178 3613
BstYI r'GATC_y
Cuts at: 1 317 816 1283 1306 1641 1887 2899 2985
Size: 316 499 467 23 335 246 1012 86
Cuts at: 2985 2997 3343 1
Size: 12 346 449
Fragments arranged by size:
1012 499 467 449 346 335 316 246
86 23 12
BtgI C'CryG_G
Cuts at: 737 737
Size: 3791
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1741 1922 2085 1741
Size: 181 163 3447
Fragments arranged by size:
3447 181 163
BtsI GCAGTG_nn'
Cuts at: 257 493 1034 257
Size: 236 541 3014
Fragments arranged by size:
3014 541 236
DraI TTT'AAA
Cuts at: 790 790
Size: 3791
DrdI GACnn_nn'nnGTC
Cuts at: 1497 2355 1497
Size: 858 2933
EaeI y'GGCC_r
Cuts at: 236 479 519 1376 1550 1941 1968 3313 236
Size: 243 40 857 174 391 27 1345 714
Fragments arranged by size:
1345 857 714 391 243 174 40 27
EagI C'GGCC_G
Cuts at: 1376 1376
Size: 3791
EarI CTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1999 2309 2455 3601 1999
Size: 310 146 1146 2189
Fragments arranged by size:
2189 1146 310 146
EcoICRI GAG'CTC
Cuts at: 716 803 716
Size: 87 3704
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1616 2048 2795 3063 1616
Size: 432 747 268 2344
Fragments arranged by size:
2344 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 755 930 755
Size: 175 3616
EcoRI G'AATT_C
Cuts at: 795 795
Size: 3791
EcoRV GAT'ATC
Cuts at: 125 125
Size: 3791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1810 1810
Size: 3791
FspI TGC'GCA
Cuts at: 1572 1572
Size: 3791
HaeII r_GCGC'y
Cuts at: 1225 1473 2495 3474 1225
Size: 248 1022 979 1542
Fragments arranged by size:
1542 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1633 1663 1665 1695 1633
Size: 30 2 30 3729
Fragments arranged by size:
3729 30 30 2
HincII GTy'rAC
Cuts at: 830 830
Size: 3791
KasI G'GCGC_C
Cuts at: 1221 1469 1221
Size: 248 3543
KpnI G_GTAC'C
Cuts at: 477 811 477
Size: 334 3457
MluI A'CGCG_T
Cuts at: 3224 3224
Size: 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2462 2646 2462
Size: 184 3607
MscI TGG'CCA
Cuts at: 238 481 521 1552 238
Size: 243 40 1031 2477
Fragments arranged by size:
2477 1031 243 40
MslI CAynn'nnrTG
Cuts at: 538 739 1780 1907 538
Size: 201 1041 127 2422
Fragments arranged by size:
2422 1041 201 127
MspA1I CmG'CkG
Cuts at: 902 1216 1576 2589 2834 902
Size: 314 360 1013 245 1859
Fragments arranged by size:
1859 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1972 1972
Size: 3791
NarI GG'CG_CC
Cuts at: 1222 1470 1222
Size: 248 3543
NcoI C'CATG_G
Cuts at: 737 737
Size: 3791
NgoMIV G'CCGG_C
Cuts at: 1970 1970
Size: 3791
NspI r_CATG'y
Cuts at: 351 700 844 1344 2251 351
Size: 349 144 500 907 1891
Fragments arranged by size:
1891 907 500 349 144
PasI CC'CwG_GG
Cuts at: 426 426
Size: 3791
PciI A'CATG_T
Cuts at: 2247 2247
Size: 3791
PmeI GTTT'AAAC
Cuts at: 790 790
Size: 3791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2930 2962 2930
Size: 32 3759
PpuMI rG'GwC_Cy
Cuts at: 755 755
Size: 3791
PstI C_TGCA'G
Cuts at: 838 1523 838
Size: 685 3106
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3791
PvuII CAG'CTG
Cuts at: 1216 1576 1216
Size: 360 3431
RsrII CG'GwC_CG
Cuts at: 1986 1986
Size: 3791
SacI G_AGCT'C
Cuts at: 718 805 718
Size: 87 3704
SalI G'TCGA_C
Cuts at: 828 828
Size: 3791
SapI GCTCTTCn'nnn_
Cuts at: 1814 2024 1814
Size: 210 3581
SbfI CC_TGCA'GG
Cuts at: 838 838
Size: 3791
ScaI AGT'ACT
Cuts at: 3308 3308
Size: 3791
SfcI C'TryA_G
Cuts at: 33 834 1519 2512 2703 33
Size: 801 685 993 191 1121
Fragments arranged by size:
1121 993 801 685 191
SfoI GGC'GCC
Cuts at: 1223 1471 1223
Size: 248 3543
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3791
SmaI CCC'GGG
Cuts at: 813 813
Size: 3791
SmlI C'TyrA_G
Cuts at: 946 1861 2353 2615 946
Size: 915 492 262 2122
Fragments arranged by size:
2122 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3791
SphI G_CATG'C
Cuts at: 844 1344 844
Size: 500 3291
StyI C'CwwG_G
Cuts at: 567 737 750 764 925 1279 567
Size: 170 13 14 161 354 3079
Fragments arranged by size:
3079 354 170 161 14 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 506 1374 1507 1966 2175 506
Size: 868 133 459 209 2122
Fragments arranged by size:
2122 868 459 209 133
TatI w'GTAC_w
Cuts at: 743 3306 743
Size: 2563 1228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 490 522 3308 3340 490
Size: 32 2786 32 941
Fragments arranged by size:
2786 941 32 32
Tth111I GACn'n_nGTC
Cuts at: 1588 1588
Size: 3791
XbaI T'CTAG_A
Cuts at: 822 822
Size: 3791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1286 1286
Size: 3791
XmaI C'CCGG_G
Cuts at: 811 811
Size: 3791
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BseRI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI