Restriction map for
pF1KE0815
(Circular) (Six-base) MAPSORT of: pF1KE0815.seq Check: 4277 from: 1 to: 3770
pF1KE0815 3770 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 808 808
Size: 3770
Acc65I G'GTAC_C
Cuts at: 786 786
Size: 3770
AclI AA'CG_TT
Cuts at: 3610 3610
Size: 3770
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 599 1595 2027 2774 3042 515
Size: 84 996 432 747 268 1243
Fragments arranged by size:
1243 996 747 432 268 84
AflIII A'CryG_T
Cuts at: 1122 2226 3203 1122
Size: 1104 977 1689
Fragments arranged by size:
1689 1104 977
AgeI A'CCGG_T
Cuts at: 2158 2158
Size: 3770
AlwNI CAG_nnn'CTG
Cuts at: 622 1075 2642 622
Size: 453 1567 1750
Fragments arranged by size:
1750 1567 453
ApaLI G'TGCA_C
Cuts at: 2540 2540
Size: 3770
ApoI r'AATT_y
Cuts at: 126 774 3123 3379 126
Size: 648 2349 256 517
Fragments arranged by size:
2349 648 517 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3770
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
AvaI C'yCGr_G
Cuts at: 790 790
Size: 3770
BamHI G'GATC_C
Cuts at: 795 795
Size: 3770
BanI G'GyrC_C
Cuts at: 553 786 1200 1448 1483 553
Size: 233 414 248 35 2840
Fragments arranged by size:
2840 414 248 233 35
BanII G_rGCy'C
Cuts at: 784 1814 784
Size: 1030 2740
BbeI G_GCGC'C
Cuts at: 1204 1452 1204
Size: 248 3522
BbsI GAAGACnn'nnnn_
Cuts at: 503 534 503
Size: 31 3739
BciVI GTATCCnnnnn_n'
Cuts at: 1668 2429 3770 1668
Size: 761 1341 1668
Fragments arranged by size:
1668 1341 761
BclI T'GATC_A
Cuts at: 1290 1290
Size: 3770
BfrBI ATG'CAT
Cuts at: 326 326
Size: 3770
BglII A'GATC_T
Cuts at: 1285 3322 1285
Size: 2037 1733
BlpI GC'TnA_GC
Cuts at: 882 882
Size: 3770
Bme1580I G_kGCm'C
Cuts at: 1395 1488 2544 1395
Size: 93 1056 2621
Fragments arranged by size:
2621 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 721 982 1063 1398 3542 721
Size: 261 81 335 2144 949
Fragments arranged by size:
2144 949 335 261 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 3770
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 946 1825 2317 2615 946
Size: 879 492 298 2101
Fragments arranged by size:
2101 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3474 3474
Size: 3770
BsaAI yAC'GTr
Cuts at: 1123 1123
Size: 3770
BsaBI GATnn'nnATC
Cuts at: 6 1284 3321 6
Size: 1278 2037 455
Fragments arranged by size:
2037 1278 455
BsaHI Gr'CG_yC
Cuts at: 1201 1449 2151 3647 1201
Size: 248 702 1496 1324
Fragments arranged by size:
1496 1324 702 248
BsaWI w'CCGG_w
Cuts at: 959 1480 2158 2432 2579 2990 959
Size: 521 678 274 147 411 1739
Fragments arranged by size:
1739 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 169 199 169
Size: 30 3740
BseYI C'CCAG_C
Cuts at: 512 1195 1953 2530 3026 512
Size: 683 758 577 496 1256
Fragments arranged by size:
1256 758 683 577 496
BsiEI CG_ry'CG
Cuts at: 104 1312 1358 2566 104
Size: 1208 46 1208 1308
Fragments arranged by size:
1308 1208 1208 46
BsiHKAI G_wGCw'C
Cuts at: 784 1562 1752 2544 784
Size: 778 190 792 2010
Fragments arranged by size:
2010 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 784 1395 1488 1562 1752 1814 2544 784
Size: 611 93 74 190 62 730 2010
Fragments arranged by size:
2010 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2990 2990
Size: 3770
BspHI T'CATG_A
Cuts at: 209 329 2946 209
Size: 120 2617 1033
Fragments arranged by size:
2617 1033 120
BspMI ACCTGCnnnn'nnnn_
Cuts at: 820 1034 1336 1717 820
Size: 214 302 381 2873
Fragments arranged by size:
2873 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2062 2116 3600 3765 50
Size: 2012 54 1484 165 55
Fragments arranged by size:
2012 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1682 1682
Size: 3770
BsrFI r'CCGG_y
Cuts at: 1768 1949 2158 1768
Size: 181 209 3380
Fragments arranged by size:
3380 209 181
BsrGI T'GTAC_A
Cuts at: 110 110
Size: 3770
BssSI C'ACGA_G
Cuts at: 2041 2399 2041
Size: 358 3412
BstAPI GCAn_nnn'nTGC
Cuts at: 1147 1147
Size: 3770
BstBI TT'CG_AA
Cuts at: 2131 2131
Size: 3770
BstYI r'GATC_y
Cuts at: 1 795 1262 1285 1620 1866 2878 2964 2976
Size: 794 467 23 335 246 1012 86 12
Cuts at: 2976 3322 1
Size: 346 449
Fragments arranged by size:
1012 794 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1720 1901 2064 1720
Size: 181 163 3426
Fragments arranged by size:
3426 181 163
BtsI GCAGTG_nn'
Cuts at: 1013 1013
Size: 3770
DraI TTT'AAA
Cuts at: 769 769
Size: 3770
DrdI GACnn_nn'nnGTC
Cuts at: 1476 2334 1476
Size: 858 2912
EaeI y'GGCC_r
Cuts at: 173 1355 1529 1920 1947 3292 173
Size: 1182 174 391 27 1345 651
Fragments arranged by size:
1345 1182 651 391 174 27
EagI C'GGCC_G
Cuts at: 1355 1355
Size: 3770
EarI CTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1978 2288 2434 3580 1978
Size: 310 146 1146 2168
Fragments arranged by size:
2168 1146 310 146
EcoICRI GAG'CTC
Cuts at: 782 782
Size: 3770
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 599 1595 2027 2774 3042 515
Size: 84 996 432 747 268 1243
Fragments arranged by size:
1243 996 747 432 268 84
EcoNI CCTnn'n_nnAGG
Cuts at: 285 285
Size: 3770
EcoO109I rG'GnC_Cy
Cuts at: 909 909
Size: 3770
EcoRI G'AATT_C
Cuts at: 774 774
Size: 3770
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1789 1789
Size: 3770
FspI TGC'GCA
Cuts at: 1551 1551
Size: 3770
HaeII r_GCGC'y
Cuts at: 1204 1452 2474 3453 1204
Size: 248 1022 979 1521
Fragments arranged by size:
1521 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1612 1642 1644 1674 1612
Size: 30 2 30 3708
Fragments arranged by size:
3708 30 30 2
HincII GTy'rAC
Cuts at: 809 809
Size: 3770
HindIII A'AGCT_T
Cuts at: 635 635
Size: 3770
KasI G'GCGC_C
Cuts at: 1200 1448 1200
Size: 248 3522
KpnI G_GTAC'C
Cuts at: 790 790
Size: 3770
MluI A'CGCG_T
Cuts at: 3203 3203
Size: 3770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2441 2625 2441
Size: 184 3586
MscI TGG'CCA
Cuts at: 175 1531 175
Size: 1356 2414
MslI CAynn'nnrTG
Cuts at: 372 697 1759 1886 372
Size: 325 1062 127 2256
Fragments arranged by size:
2256 1062 325 127
MspA1I CmG'CkG
Cuts at: 881 1195 1555 2568 2813 881
Size: 314 360 1013 245 1838
Fragments arranged by size:
1838 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1951 1951
Size: 3770
NarI GG'CG_CC
Cuts at: 1201 1449 1201
Size: 248 3522
NgoMIV G'CCGG_C
Cuts at: 1949 1949
Size: 3770
NsiI A_TGCA'T
Cuts at: 328 328
Size: 3770
NspI r_CATG'y
Cuts at: 823 1323 2230 823
Size: 500 907 2363
Fragments arranged by size:
2363 907 500
PciI A'CATG_T
Cuts at: 2226 2226
Size: 3770
PfoI T'CCnGG_A
Cuts at: 137 711 137
Size: 574 3196
PmeI GTTT'AAAC
Cuts at: 769 769
Size: 3770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 3738
PstI C_TGCA'G
Cuts at: 817 1502 817
Size: 685 3085
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3770
PvuII CAG'CTG
Cuts at: 1195 1555 1195
Size: 360 3410
RsrII CG'GwC_CG
Cuts at: 1965 1965
Size: 3770
SacI G_AGCT'C
Cuts at: 784 784
Size: 3770
SalI G'TCGA_C
Cuts at: 807 807
Size: 3770
SapI GCTCTTCn'nnn_
Cuts at: 1793 2003 1793
Size: 210 3560
SbfI CC_TGCA'GG
Cuts at: 817 817
Size: 3770
ScaI AGT'ACT
Cuts at: 3287 3287
Size: 3770
SfcI C'TryA_G
Cuts at: 33 813 1498 2491 2682 33
Size: 780 685 993 191 1121
Fragments arranged by size:
1121 993 780 685 191
SfoI GGC'GCC
Cuts at: 1202 1450 1202
Size: 248 3522
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3770
SmaI CCC'GGG
Cuts at: 792 792
Size: 3770
SmlI C'TyrA_G
Cuts at: 925 1840 2332 2594 925
Size: 915 492 262 2101
Fragments arranged by size:
2101 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3770
SphI G_CATG'C
Cuts at: 823 1323 823
Size: 500 3270
SspI AAT'ATT
Cuts at: 441 441
Size: 3770
StyI C'CwwG_G
Cuts at: 904 1258 904
Size: 354 3416
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 526 740 1353 1486 1945 2154 526
Size: 214 613 133 459 209 2142
Fragments arranged by size:
2142 613 459 214 209 133
TatI w'GTAC_w
Cuts at: 110 3285 110
Size: 3175 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3287 3319 3287
Size: 32 3738
Tth111I GACn'n_nGTC
Cuts at: 1567 1567
Size: 3770
XbaI T'CTAG_A
Cuts at: 801 801
Size: 3770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1265 1265
Size: 3770
XmaI C'CCGG_G
Cuts at: 790 790
Size: 3770
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI