Restriction map for
pF1KE0814
(Circular) (Six-base) MAPSORT of: pF1KE0814.seq Check: 4403 from: 1 to: 3881
pF1KE0814 3881 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 919 919
Size: 3881
Acc65I G'GTAC_C
Cuts at: 897 897
Size: 3881
AclI AA'CG_TT
Cuts at: 3721 3721
Size: 3881
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1706 2138 2885 3153 277
Size: 1429 432 747 268 1005
Fragments arranged by size:
1429 1005 747 432 268
AflIII A'CryG_T
Cuts at: 1233 2337 3314 1233
Size: 1104 977 1800
Fragments arranged by size:
1800 1104 977
AgeI A'CCGG_T
Cuts at: 2269 2269
Size: 3881
AlwNI CAG_nnn'CTG
Cuts at: 1186 2753 1186
Size: 1567 2314
ApaLI G'TGCA_C
Cuts at: 2651 2651
Size: 3881
ApoI r'AATT_y
Cuts at: 885 3234 3490 885
Size: 2349 256 1276
Fragments arranged by size:
2349 1276 256
AseI AT'TA_AT
Cuts at: 20 142 20
Size: 122 3759
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
AvaI C'yCGr_G
Cuts at: 901 901
Size: 3881
BamHI G'GATC_C
Cuts at: 869 906 869
Size: 37 3844
BanI G'GyrC_C
Cuts at: 897 1311 1559 1594 897
Size: 414 248 35 3184
Fragments arranged by size:
3184 414 248 35
BanII G_rGCy'C
Cuts at: 895 1925 895
Size: 1030 2851
BbeI G_GCGC'C
Cuts at: 1315 1563 1315
Size: 248 3633
BbvCI CC'TCA_GC
Cuts at: 376 376
Size: 3881
BciVI GTATCCnnnnn_n'
Cuts at: 1779 2540 3881 1779
Size: 761 1341 1779
Fragments arranged by size:
1779 1341 761
BclI T'GATC_A
Cuts at: 1401 1401
Size: 3881
BglI GCCn_nnn'nGGC
Cuts at: 541 541
Size: 3881
BglII A'GATC_T
Cuts at: 1396 3433 1396
Size: 2037 1844
BlpI GC'TnA_GC
Cuts at: 993 993
Size: 3881
Bme1580I G_kGCm'C
Cuts at: 1506 1599 2655 1506
Size: 93 1056 2732
Fragments arranged by size:
2732 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1093 1174 1509 3653 1093
Size: 81 335 2144 1321
Fragments arranged by size:
2144 1321 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 3881
Bpu10I CC'TnA_GC
Cuts at: 376 376
Size: 3881
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1936 2428 2726 1057
Size: 879 492 298 2212
Fragments arranged by size:
2212 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3585 3585
Size: 3881
BsaAI yAC'GTr
Cuts at: 1234 1234
Size: 3881
BsaBI GATnn'nnATC
Cuts at: 6 1395 3432 6
Size: 1389 2037 455
Fragments arranged by size:
2037 1389 455
BsaHI Gr'CG_yC
Cuts at: 1312 1560 2262 3758 1312
Size: 248 702 1496 1435
Fragments arranged by size:
1496 1435 702 248
BsaWI w'CCGG_w
Cuts at: 1070 1591 2269 2543 2690 3101 1070
Size: 521 678 274 147 411 1850
Fragments arranged by size:
1850 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1306 2064 2641 3137 1306
Size: 758 577 496 2050
Fragments arranged by size:
2050 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 505
Size: 3881
BsiEI CG_ry'CG
Cuts at: 104 552 1423 1469 2677 104
Size: 448 871 46 1208 1308
Fragments arranged by size:
1308 1208 871 448 46
BsiHKAI G_wGCw'C
Cuts at: 620 895 1673 1863 2655 620
Size: 275 778 190 792 1846
Fragments arranged by size:
1846 792 778 275 190
BsmI GAATG_Cn'
Cuts at: 326 434 326
Size: 108 3773
Bsp1286I G_dGCh'C
Cuts at: 620 895 1506 1599 1673 1863 1925 2655 620
Size: 275 611 93 74 190 62 730 1846
Fragments arranged by size:
1846 730 611 275 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3101 3101
Size: 3881
BspHI T'CATG_A
Cuts at: 3057 3057
Size: 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 931 1145 1447 1828 931
Size: 214 302 381 2984
Fragments arranged by size:
2984 381 302 214
BsrBI CCG'CTC
Cuts at: 50 453 2173 2227 3711 3876 50
Size: 403 1720 54 1484 165 55
Fragments arranged by size:
1720 1484 403 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1793 1793
Size: 3881
BsrFI r'CCGG_y
Cuts at: 1879 2060 2269 1879
Size: 181 209 3491
Fragments arranged by size:
3491 209 181
BssSI C'ACGA_G
Cuts at: 2152 2510 2152
Size: 358 3523
BstAPI GCAn_nnn'nTGC
Cuts at: 1258 1258
Size: 3881
BstBI TT'CG_AA
Cuts at: 2242 2242
Size: 3881
BstXI CCAn_nnnn'nTGG
Cuts at: 303 303
Size: 3881
BstYI r'GATC_y
Cuts at: 1 869 906 1373 1396 1731 1977 2989 3075
Size: 868 37 467 23 335 246 1012 86
Cuts at: 3075 3087 3433 1
Size: 12 346 449
Fragments arranged by size:
1012 868 467 449 346 335 246 86
37 23 12
Bsu36I CC'TnA_GG
Cuts at: 865 865
Size: 3881
BtgI C'CryG_G
Cuts at: 107 188 107
Size: 81 3800
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1831 2012 2175 1831
Size: 181 163 3537
Fragments arranged by size:
3537 181 163
BtsI GCAGTG_nn'
Cuts at: 1124 1124
Size: 3881
DraI TTT'AAA
Cuts at: 880 880
Size: 3881
DrdI GACnn_nn'nnGTC
Cuts at: 1587 2445 1587
Size: 858 3023
EaeI y'GGCC_r
Cuts at: 533 560 1466 1640 2031 2058 3403 533
Size: 27 906 174 391 27 1345 1011
Fragments arranged by size:
1345 1011 906 391 174 27 27
EagI C'GGCC_G
Cuts at: 1466 1466
Size: 3881
EarI CTCTTCn'nnn_
Cuts at: 274 1904 2114 274
Size: 1630 210 2041
Fragments arranged by size:
2041 1630 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2089 2399 2545 3691 2089
Size: 310 146 1146 2279
Fragments arranged by size:
2279 1146 310 146
EcoICRI GAG'CTC
Cuts at: 893 893
Size: 3881
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 1706 2138 2885 3153 277
Size: 1429 432 747 268 1005
Fragments arranged by size:
1429 1005 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1020 1020
Size: 3881
EcoRI G'AATT_C
Cuts at: 885 885
Size: 3881
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1900 1900
Size: 3881
FspI TGC'GCA
Cuts at: 1662 1662
Size: 3881
HaeII r_GCGC'y
Cuts at: 1315 1563 2585 3564 1315
Size: 248 1022 979 1632
Fragments arranged by size:
1632 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 632 664 1723 1753 1755 1785 632
Size: 32 1059 30 2 30 2728
Fragments arranged by size:
2728 1059 32 30 30 2
HincII GTy'rAC
Cuts at: 920 920
Size: 3881
KasI G'GCGC_C
Cuts at: 1311 1559 1311
Size: 248 3633
KpnI G_GTAC'C
Cuts at: 901 901
Size: 3881
MfeI C'AATT_G
Cuts at: 668 668
Size: 3881
MluI A'CGCG_T
Cuts at: 3314 3314
Size: 3881
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2552 2736 2552
Size: 184 3697
MscI TGG'CCA
Cuts at: 535 562 1642 535
Size: 27 1080 2774
Fragments arranged by size:
2774 1080 27
MslI CAynn'nnrTG
Cuts at: 361 1870 1997 361
Size: 1509 127 2245
Fragments arranged by size:
2245 1509 127
MspA1I CmG'CkG
Cuts at: 380 992 1306 1666 2679 2924 380
Size: 612 314 360 1013 245 1337
Fragments arranged by size:
1337 1013 612 360 314 245
NaeI GCC'GGC
Cuts at: 2062 2062
Size: 3881
NarI GG'CG_CC
Cuts at: 1312 1560 1312
Size: 248 3633
NcoI C'CATG_G
Cuts at: 107 188 107
Size: 81 3800
NgoMIV G'CCGG_C
Cuts at: 2060 2060
Size: 3881
NspI r_CATG'y
Cuts at: 479 934 1434 2341 479
Size: 455 500 907 2019
Fragments arranged by size:
2019 907 500 455
PciI A'CATG_T
Cuts at: 2337 2337
Size: 3881
PmeI GTTT'AAAC
Cuts at: 880 880
Size: 3881
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3020 3052 3020
Size: 32 3849
PstI C_TGCA'G
Cuts at: 928 1613 928
Size: 685 3196
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3881
PvuII CAG'CTG
Cuts at: 380 1306 1666 380
Size: 926 360 2595
Fragments arranged by size:
2595 926 360
RsrII CG'GwC_CG
Cuts at: 2076 2076
Size: 3881
SacI G_AGCT'C
Cuts at: 895 895
Size: 3881
SalI G'TCGA_C
Cuts at: 918 918
Size: 3881
SapI GCTCTTCn'nnn_
Cuts at: 1904 2114 1904
Size: 210 3671
SbfI CC_TGCA'GG
Cuts at: 928 928
Size: 3881
ScaI AGT'ACT
Cuts at: 3398 3398
Size: 3881
SfcI C'TryA_G
Cuts at: 33 924 1609 2602 2793 33
Size: 891 685 993 191 1121
Fragments arranged by size:
1121 993 891 685 191
SfoI GGC'GCC
Cuts at: 1313 1561 1313
Size: 248 3633
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3881
SmaI CCC'GGG
Cuts at: 903 903
Size: 3881
SmlI C'TyrA_G
Cuts at: 1036 1951 2443 2705 1036
Size: 915 492 262 2212
Fragments arranged by size:
2212 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3881
SphI G_CATG'C
Cuts at: 934 1434 934
Size: 500 3381
StyI C'CwwG_G
Cuts at: 107 188 1015 1369 107
Size: 81 827 354 2619
Fragments arranged by size:
2619 827 354 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 375 438 1464 1597 2056 2265 375
Size: 63 1026 133 459 209 1991
Fragments arranged by size:
1991 1026 459 209 133 63
TatI w'GTAC_w
Cuts at: 3396 3396
Size: 3881
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 3849
Tth111I GACn'n_nGTC
Cuts at: 1678 1678
Size: 3881
XbaI T'CTAG_A
Cuts at: 912 912
Size: 3881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 303 1376 303
Size: 1073 2808
XmaI C'CCGG_G
Cuts at: 901 901
Size: 3881
XmnI GAAnn'nnTTC
Cuts at: 143 143
Size: 3881
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BcgI BfrBI BmgBI
BmtI BpmI BsaXI BseRI BsiWI BsmBI BsrGI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI