Restriction map for
pF1KE0810
(Circular) (Six-base) MAPSORT of: pF1KE0810.seq Check: 9660 from: 1 to: 4028
pF1KE0810 4028 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1066 1066
Size: 4028
Acc65I G'GTAC_C
Cuts at: 549 1044 549
Size: 495 3533
AclI AA'CG_TT
Cuts at: 3868 3868
Size: 4028
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1853 2285 3032 3300 1853
Size: 432 747 268 2581
Fragments arranged by size:
2581 747 432 268
AflIII A'CryG_T
Cuts at: 401 1380 2484 3461 401
Size: 979 1104 977 968
Fragments arranged by size:
1104 979 977 968
AgeI A'CCGG_T
Cuts at: 2416 2416
Size: 4028
AleI CACnn'nnGTG
Cuts at: 637 637
Size: 4028
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 890 951 890
Size: 61 3967
AlwNI CAG_nnn'CTG
Cuts at: 1333 2900 1333
Size: 1567 2461
ApaLI G'TGCA_C
Cuts at: 2798 2798
Size: 4028
ApoI r'AATT_y
Cuts at: 132 373 481 610 1032 3381 3637 132
Size: 241 108 129 422 2349 256 523
Fragments arranged by size:
2349 523 422 256 241 129 108
AseI AT'TA_AT
Cuts at: 20 20
Size: 4028
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
AvaI C'yCGr_G
Cuts at: 1048 1048
Size: 4028
BamHI G'GATC_C
Cuts at: 1053 1053
Size: 4028
BanI G'GyrC_C
Cuts at: 549 1044 1458 1706 1741 549
Size: 495 414 248 35 2836
Fragments arranged by size:
2836 495 414 248 35
BanII G_rGCy'C
Cuts at: 777 1042 2072 777
Size: 265 1030 2733
Fragments arranged by size:
2733 1030 265
BbeI G_GCGC'C
Cuts at: 1462 1710 1462
Size: 248 3780
BbsI GAAGACnn'nnnn_
Cuts at: 349 693 702 349
Size: 344 9 3675
Fragments arranged by size:
3675 344 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3994
BciVI GTATCCnnnnn_n'
Cuts at: 1926 2687 4028 1926
Size: 761 1341 1926
Fragments arranged by size:
1926 1341 761
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4028
BglII A'GATC_T
Cuts at: 1543 3580 1543
Size: 2037 1991
BlpI GC'TnA_GC
Cuts at: 776 1140 776
Size: 364 3664
Bme1580I G_kGCm'C
Cuts at: 1653 1746 2802 1653
Size: 93 1056 2879
Fragments arranged by size:
2879 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1240 1321 1656 3800 1240
Size: 81 335 2144 1468
Fragments arranged by size:
2144 1468 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 4028
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 111
Size: 4028
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 936 1204 2083 2575 2873 936
Size: 268 879 492 298 2091
Fragments arranged by size:
2091 879 492 298 268
BsaI GGTCTCn'nnnn_
Cuts at: 3732 3732
Size: 4028
BsaAI yAC'GTr
Cuts at: 1381 1381
Size: 4028
BsaBI GATnn'nnATC
Cuts at: 6 1542 3579 6
Size: 1536 2037 455
Fragments arranged by size:
2037 1536 455
BsaHI Gr'CG_yC
Cuts at: 1459 1707 2409 3905 1459
Size: 248 702 1496 1582
Fragments arranged by size:
1582 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1217 1738 2416 2690 2837 3248 1217
Size: 521 678 274 147 411 1997
Fragments arranged by size:
1997 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 109 139 109
Size: 30 3998
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 610 610
Size: 4028
BseYI C'CCAG_C
Cuts at: 1453 2211 2788 3284 1453
Size: 758 577 496 2197
Fragments arranged by size:
2197 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 751 376
Size: 375 3653
BsiEI CG_ry'CG
Cuts at: 104 1570 1616 2824 104
Size: 1466 46 1208 1308
Fragments arranged by size:
1466 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 602 1042 1820 2010 2802 602
Size: 440 778 190 792 1828
Fragments arranged by size:
1828 792 778 440 190
BsmI GAATG_Cn'
Cuts at: 867 867
Size: 4028
Bsp1286I G_dGCh'C
Cuts at: 602 777 1042 1653 1746 1820 2010 2072 2802
Size: 175 265 611 93 74 190 62 730
Cuts at: 2802 602
Size: 1828
Fragments arranged by size:
1828 730 611 265 190 175 93 74
62
BspEI T'CCGG_A
Cuts at: 3248 3248
Size: 4028
BspHI T'CATG_A
Cuts at: 3204 3204
Size: 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1078 1292 1594 1975 1078
Size: 214 302 381 3131
Fragments arranged by size:
3131 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2320 2374 3858 4023 50
Size: 2270 54 1484 165 55
Fragments arranged by size:
2270 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1940 1940
Size: 4028
BsrFI r'CCGG_y
Cuts at: 2026 2207 2416 2026
Size: 181 209 3638
Fragments arranged by size:
3638 209 181
BssSI C'ACGA_G
Cuts at: 2299 2657 2299
Size: 358 3670
BstAPI GCAn_nnn'nTGC
Cuts at: 1405 1405
Size: 4028
BstBI TT'CG_AA
Cuts at: 2389 2389
Size: 4028
BstEII G'GTnAC_C
Cuts at: 438 438
Size: 4028
BstYI r'GATC_y
Cuts at: 1 842 1053 1520 1543 1878 2124 3136 3222
Size: 841 211 467 23 335 246 1012 86
Cuts at: 3222 3234 3580 1
Size: 12 346 449
Fragments arranged by size:
1012 841 467 449 346 335 246 211
86 23 12
BtgI C'CryG_G
Cuts at: 263 263
Size: 4028
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1978 2159 2322 1978
Size: 181 163 3684
Fragments arranged by size:
3684 181 163
BtsI GCAGTG_nn'
Cuts at: 1271 1271
Size: 4028
DraI TTT'AAA
Cuts at: 609 1027 609
Size: 418 3610
DrdI GACnn_nn'nnGTC
Cuts at: 1734 2592 1734
Size: 858 3170
EaeI y'GGCC_r
Cuts at: 114 1613 1787 2178 2205 3550 114
Size: 1499 174 391 27 1345 592
Fragments arranged by size:
1499 1345 592 391 174 27
EagI C'GGCC_G
Cuts at: 1613 1613
Size: 4028
EarI CTCTTCn'nnn_
Cuts at: 2051 2261 2051
Size: 210 3818
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2236 2546 2692 3838 2236
Size: 310 146 1146 2426
Fragments arranged by size:
2426 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1040 1040
Size: 4028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 111 1853 2285 3032 3300 111
Size: 1742 432 747 268 839
Fragments arranged by size:
1742 839 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1167 1167
Size: 4028
EcoRI G'AATT_C
Cuts at: 132 481 1032 132
Size: 349 551 3128
Fragments arranged by size:
3128 551 349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 4028
FspI TGC'GCA
Cuts at: 1809 1809
Size: 4028
HaeII r_GCGC'y
Cuts at: 1462 1710 2732 3711 1462
Size: 248 1022 979 1779
Fragments arranged by size:
1779 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 437 469 1870 1900 1902 1932 437
Size: 32 1401 30 2 30 2533
Fragments arranged by size:
2533 1401 32 30 30 2
HincII GTy'rAC
Cuts at: 1067 1067
Size: 4028
KasI G'GCGC_C
Cuts at: 1458 1706 1458
Size: 248 3780
KpnI G_GTAC'C
Cuts at: 553 1048 553
Size: 495 3533
MluI A'CGCG_T
Cuts at: 3461 3461
Size: 4028
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2699 2883 2699
Size: 184 3844
MscI TGG'CCA
Cuts at: 116 1789 116
Size: 1673 2355
MslI CAynn'nnrTG
Cuts at: 400 637 718 872 2017 2144 400
Size: 237 81 154 1145 127 2284
Fragments arranged by size:
2284 1145 237 154 127 81
MspA1I CmG'CkG
Cuts at: 780 1139 1453 1813 2826 3071 780
Size: 359 314 360 1013 245 1737
Fragments arranged by size:
1737 1013 360 359 314 245
NaeI GCC'GGC
Cuts at: 2209 2209
Size: 4028
NarI GG'CG_CC
Cuts at: 1459 1707 1459
Size: 248 3780
NcoI C'CATG_G
Cuts at: 263 263
Size: 4028
NdeI CA'TA_TG
Cuts at: 636 636
Size: 4028
NgoMIV G'CCGG_C
Cuts at: 2207 2207
Size: 4028
NspI r_CATG'y
Cuts at: 405 502 525 1081 1581 2488 405
Size: 97 23 556 500 907 1945
Fragments arranged by size:
1945 907 556 500 97 23
PciI A'CATG_T
Cuts at: 401 2484 401
Size: 2083 1945
PfoI T'CCnGG_A
Cuts at: 127 127
Size: 4028
PmeI GTTT'AAAC
Cuts at: 1027 1027
Size: 4028
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3167 3199 3167
Size: 32 3996
PsiI TTA'TAA
Cuts at: 902 902
Size: 4028
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 397 429 397
Size: 32 3996
PstI C_TGCA'G
Cuts at: 1075 1760 1075
Size: 685 3343
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4028
PvuII CAG'CTG
Cuts at: 780 1453 1813 780
Size: 673 360 2995
Fragments arranged by size:
2995 673 360
RsrII CG'GwC_CG
Cuts at: 2223 2223
Size: 4028
SacI G_AGCT'C
Cuts at: 1042 1042
Size: 4028
SalI G'TCGA_C
Cuts at: 1065 1065
Size: 4028
SapI GCTCTTCn'nnn_
Cuts at: 2051 2261 2051
Size: 210 3818
SbfI CC_TGCA'GG
Cuts at: 1075 1075
Size: 4028
ScaI AGT'ACT
Cuts at: 3545 3545
Size: 4028
SfcI C'TryA_G
Cuts at: 33 149 486 1071 1756 2749 2940 33
Size: 116 337 585 685 993 191 1121
Fragments arranged by size:
1121 993 685 585 337 191 116
SfoI GGC'GCC
Cuts at: 1460 1708 1460
Size: 248 3780
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4028
SmaI CCC'GGG
Cuts at: 1050 1050
Size: 4028
SmlI C'TyrA_G
Cuts at: 915 1183 2098 2590 2852 915
Size: 268 915 492 262 2091
Fragments arranged by size:
2091 915 492 268 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4028
SphI G_CATG'C
Cuts at: 502 1081 1581 502
Size: 579 500 2949
Fragments arranged by size:
2949 579 500
StyI C'CwwG_G
Cuts at: 263 1162 1516 263
Size: 899 354 2775
Fragments arranged by size:
2775 899 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 196 1611 1744 2203 2412 196
Size: 1415 133 459 209 1812
Fragments arranged by size:
1812 1415 459 209 133
TatI w'GTAC_w
Cuts at: 3543 3543
Size: 4028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 109 141 3545 3577 109
Size: 32 3404 32 560
Fragments arranged by size:
3404 560 32 32
Tth111I GACn'n_nGTC
Cuts at: 1825 1825
Size: 4028
XbaI T'CTAG_A
Cuts at: 1059 1059
Size: 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1523 1523
Size: 4028
XmaI C'CCGG_G
Cuts at: 1048 1048
Size: 4028
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PfoI PmeI PpiI
PsiI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PspOMI PspXI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI