Restriction map for
pF1KE0808
(Circular) (Six-base) MAPSORT of: pF1KE0808.seq Check: 8660 from: 1 to: 3956
pF1KE0808 3956 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 494 994 494
Size: 500 3456
Acc65I G'GTAC_C
Cuts at: 972 972
Size: 3956
AclI AA'CG_TT
Cuts at: 3796 3796
Size: 3956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1781 2213 2960 3228 1781
Size: 432 747 268 2509
Fragments arranged by size:
2509 747 432 268
AflIII A'CryG_T
Cuts at: 1308 2412 3389 1308
Size: 1104 977 1875
Fragments arranged by size:
1875 1104 977
AgeI A'CCGG_T
Cuts at: 2344 2344
Size: 3956
AlwNI CAG_nnn'CTG
Cuts at: 244 343 1261 2828 244
Size: 99 918 1567 1372
Fragments arranged by size:
1567 1372 918 99
ApaLI G'TGCA_C
Cuts at: 2726 2726
Size: 3956
ApoI r'AATT_y
Cuts at: 960 3309 3565 960
Size: 2349 256 1351
Fragments arranged by size:
2349 1351 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
AvaI C'yCGr_G
Cuts at: 976 976
Size: 3956
BamHI G'GATC_C
Cuts at: 981 981
Size: 3956
BanI G'GyrC_C
Cuts at: 972 1386 1634 1669 972
Size: 414 248 35 3259
Fragments arranged by size:
3259 414 248 35
BanII G_rGCy'C
Cuts at: 182 970 2000 182
Size: 788 1030 2138
Fragments arranged by size:
2138 1030 788
BbeI G_GCGC'C
Cuts at: 1390 1638 1390
Size: 248 3708
BbsI GAAGACnn'nnnn_
Cuts at: 683 898 683
Size: 215 3741
BbvCI CC'TCA_GC
Cuts at: 210 210
Size: 3956
BciVI GTATCCnnnnn_n'
Cuts at: 1854 2615 3956 1854
Size: 761 1341 1854
Fragments arranged by size:
1854 1341 761
BclI T'GATC_A
Cuts at: 152 1476 152
Size: 1324 2632
BglI GCCn_nnn'nGGC
Cuts at: 565 565
Size: 3956
BglII A'GATC_T
Cuts at: 1471 3508 1471
Size: 2037 1919
BlpI GC'TnA_GC
Cuts at: 1068 1068
Size: 3956
Bme1580I G_kGCm'C
Cuts at: 860 1581 1674 2730 860
Size: 721 93 1056 2086
Fragments arranged by size:
2086 1056 721 93
BmrI ACTGGGnnnn_n'
Cuts at: 1168 1249 1584 3728 1168
Size: 81 335 2144 1396
Fragments arranged by size:
2144 1396 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 2323
Size: 3956
Bpu10I CC'TnA_GC
Cuts at: 210 210
Size: 3956
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1132 2011 2503 2801 1132
Size: 879 492 298 2287
Fragments arranged by size:
2287 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3660 3660
Size: 3956
BsaAI yAC'GTr
Cuts at: 725 1309 725
Size: 584 3372
BsaBI GATnn'nnATC
Cuts at: 6 1470 3507 6
Size: 1464 2037 455
Fragments arranged by size:
2037 1464 455
BsaHI Gr'CG_yC
Cuts at: 1387 1635 2337 3833 1387
Size: 248 702 1496 1510
Fragments arranged by size:
1510 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1145 1666 2344 2618 2765 3176 1145
Size: 521 678 274 147 411 1925
Fragments arranged by size:
1925 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 765 1381 2139 2716 3212 765
Size: 616 758 577 496 1509
Fragments arranged by size:
1509 758 616 577 496
BsiEI CG_ry'CG
Cuts at: 104 1498 1544 2752 104
Size: 1394 46 1208 1308
Fragments arranged by size:
1394 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 548 970 1748 1938 2730 548
Size: 422 778 190 792 1774
Fragments arranged by size:
1774 792 778 422 190
BsmI GAATG_Cn'
Cuts at: 769 769
Size: 3956
Bsp1286I G_dGCh'C
Cuts at: 182 548 860 970 1581 1674 1748 1938 2000
Size: 366 312 110 611 93 74 190 62
Cuts at: 2000 2730 182
Size: 730 1408
Fragments arranged by size:
1408 730 611 366 312 190 110 93
74 62
BspEI T'CCGG_A
Cuts at: 3176 3176
Size: 3956
BspHI T'CATG_A
Cuts at: 155 3132 155
Size: 2977 979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1006 1220 1522 1903 1006
Size: 214 302 381 3059
Fragments arranged by size:
3059 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2248 2302 3786 3951 50
Size: 2198 54 1484 165 55
Fragments arranged by size:
2198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 365 554 1868 365
Size: 189 1314 2453
Fragments arranged by size:
2453 1314 189
BsrFI r'CCGG_y
Cuts at: 1954 2135 2344 1954
Size: 181 209 3566
Fragments arranged by size:
3566 209 181
BsrGI T'GTAC_A
Cuts at: 110 110
Size: 3956
BssSI C'ACGA_G
Cuts at: 2227 2585 2227
Size: 358 3598
BstAPI GCAn_nnn'nTGC
Cuts at: 343 1333 343
Size: 990 2966
BstBI TT'CG_AA
Cuts at: 2317 2317
Size: 3956
BstXI CCAn_nnnn'nTGG
Cuts at: 796 796
Size: 3956
BstYI r'GATC_y
Cuts at: 1 981 1448 1471 1806 2052 3064 3150 3162
Size: 980 467 23 335 246 1012 86 12
Cuts at: 3162 3508 1
Size: 346 449
Fragments arranged by size:
1012 980 467 449 346 335 246 86
23 12
BstZ17I GTA'TAC
Cuts at: 495 495
Size: 3956
Bsu36I CC'TnA_GG
Cuts at: 245 245
Size: 3956
BtgI C'CryG_G
Cuts at: 275 275
Size: 3956
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 775 1906 2087 2250 775
Size: 1131 181 163 2481
Fragments arranged by size:
2481 1131 181 163
BtsI GCAGTG_nn'
Cuts at: 683 1199 683
Size: 516 3440
DraI TTT'AAA
Cuts at: 955 955
Size: 3956
DraIII CAC_nnn'GTG
Cuts at: 678 678
Size: 3956
DrdI GACnn_nn'nnGTC
Cuts at: 1662 2520 1662
Size: 858 3098
EaeI y'GGCC_r
Cuts at: 380 1541 1715 2106 2133 3478 380
Size: 1161 174 391 27 1345 858
Fragments arranged by size:
1345 1161 858 391 174 27
EagI C'GGCC_G
Cuts at: 1541 1541
Size: 3956
EarI CTCTTCn'nnn_
Cuts at: 196 1979 2189 196
Size: 1783 210 1963
Fragments arranged by size:
1963 1783 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2164 2474 2620 3766 2164
Size: 310 146 1146 2354
Fragments arranged by size:
2354 1146 310 146
EcoICRI GAG'CTC
Cuts at: 968 968
Size: 3956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1781 2213 2960 3228 1781
Size: 432 747 268 2509
Fragments arranged by size:
2509 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 714 1095 714
Size: 381 3575
EcoRI G'AATT_C
Cuts at: 960 960
Size: 3956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1975 1975
Size: 3956
FspI TGC'GCA
Cuts at: 1737 1737
Size: 3956
HaeII r_GCGC'y
Cuts at: 1390 1638 2660 3639 1390
Size: 248 1022 979 1707
Fragments arranged by size:
1707 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1798 1828 1830 1860 1798
Size: 30 2 30 3894
Fragments arranged by size:
3894 30 30 2
HincII GTy'rAC
Cuts at: 995 995
Size: 3956
KasI G'GCGC_C
Cuts at: 1386 1634 1386
Size: 248 3708
KpnI G_GTAC'C
Cuts at: 976 976
Size: 3956
MluI A'CGCG_T
Cuts at: 3389 3389
Size: 3956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2627 2811 2627
Size: 184 3772
MscI TGG'CCA
Cuts at: 382 1717 382
Size: 1335 2621
MslI CAynn'nnrTG
Cuts at: 331 740 751 832 1945 2072 331
Size: 409 11 81 1113 127 2215
Fragments arranged by size:
2215 1113 409 127 81 11
MspA1I CmG'CkG
Cuts at: 232 448 1067 1381 1741 2754 2999 232
Size: 216 619 314 360 1013 245 1189
Fragments arranged by size:
1189 1013 619 360 314 245 216
NaeI GCC'GGC
Cuts at: 2137 2137
Size: 3956
NarI GG'CG_CC
Cuts at: 1387 1635 1387
Size: 248 3708
NcoI C'CATG_G
Cuts at: 275 275
Size: 3956
NgoMIV G'CCGG_C
Cuts at: 2135 2135
Size: 3956
NspI r_CATG'y
Cuts at: 1009 1509 2416 1009
Size: 500 907 2549
Fragments arranged by size:
2549 907 500
PciI A'CATG_T
Cuts at: 2412 2412
Size: 3956
PflMI CCAn_nnn'nTGG
Cuts at: 276 276
Size: 3956
PmeI GTTT'AAAC
Cuts at: 955 955
Size: 3956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3924
PstI C_TGCA'G
Cuts at: 240 1003 1688 240
Size: 763 685 2508
Fragments arranged by size:
2508 763 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3956
PvuII CAG'CTG
Cuts at: 448 1381 1741 448
Size: 933 360 2663
Fragments arranged by size:
2663 933 360
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 3956
SacI G_AGCT'C
Cuts at: 970 970
Size: 3956
SalI G'TCGA_C
Cuts at: 993 993
Size: 3956
SapI GCTCTTCn'nnn_
Cuts at: 1979 2189 1979
Size: 210 3746
SbfI CC_TGCA'GG
Cuts at: 1003 1003
Size: 3956
ScaI AGT'ACT
Cuts at: 3473 3473
Size: 3956
SfcI C'TryA_G
Cuts at: 33 236 623 999 1684 2677 2868 33
Size: 203 387 376 685 993 191 1121
Fragments arranged by size:
1121 993 685 387 376 203 191
SfoI GGC'GCC
Cuts at: 1388 1636 1388
Size: 248 3708
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
SmaI CCC'GGG
Cuts at: 978 978
Size: 3956
SmlI C'TyrA_G
Cuts at: 1111 2026 2518 2780 1111
Size: 915 492 262 2287
Fragments arranged by size:
2287 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3956
SphI G_CATG'C
Cuts at: 1009 1509 1009
Size: 500 3456
StyI C'CwwG_G
Cuts at: 275 1090 1444 275
Size: 815 354 2787
Fragments arranged by size:
2787 815 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1539 1672 2131 2340 1539
Size: 133 459 209 3155
Fragments arranged by size:
3155 459 209 133
TatI w'GTAC_w
Cuts at: 110 3471 110
Size: 3361 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 3924
Tth111I GACn'n_nGTC
Cuts at: 1753 1753
Size: 3956
XbaI T'CTAG_A
Cuts at: 987 987
Size: 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1451 114
Size: 1337 2619
XmaI C'CCGG_G
Cuts at: 976 976
Size: 3956
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BcgI BfrBI BmgBI BmtI
BpmI BsaXI BseRI BsgI BsiWI BsmBI BssHII BstEII
ClaI CspCI EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI