Restriction map for
pF1KE0800
(Circular) (Six-base) MAPSORT of: pF1KE0800.seq Check: 3304 from: 1 to: 7706
pF1KE0800 7706 bp
With 182 enzymes: SgfI *
September 2, 2010 12:21 ..
AccI GT'mk_AC
Cuts at: 4744 4744
Size: 7706
Acc65I G'GTAC_C
Cuts at: 2479 4722 2479
Size: 2243 5463
AclI AA'CG_TT
Cuts at: 7546 7546
Size: 7706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1639 2057 2687 3047 3752 3860 5531 5963 6710
Size: 418 630 360 705 108 1671 432 747
Cuts at: 6710 6978 1639
Size: 268 2367
Fragments arranged by size:
2367 1671 747 705 630 432 418 360
268 108
AflIII A'CryG_T
Cuts at: 751 923 1080 1184 2579 5058 6162 7139 751
Size: 172 157 104 1395 2479 1104 977 1318
Fragments arranged by size:
2479 1395 1318 1104 977 172 157 104
AgeI A'CCGG_T
Cuts at: 4396 6094 4396
Size: 1698 6008
AhdI GACnn_n'nnGTC
Cuts at: 587 587
Size: 7706
AlwNI CAG_nnn'CTG
Cuts at: 2362 3469 5011 6578 2362
Size: 1107 1542 1567 3490
Fragments arranged by size:
3490 1567 1542 1107
ApaLI G'TGCA_C
Cuts at: 6476 6476
Size: 7706
ApoI r'AATT_y
Cuts at: 634 1297 1418 1822 1888 2229 2427 3007 4081
Size: 663 121 404 66 341 198 580 1074
Cuts at: 4081 4449 4710 7059 7315 634
Size: 368 261 2349 256 1025
Fragments arranged by size:
2349 1074 1025 663 580 404 368 341
261 256 198 121 66
AseI AT'TA_AT
Cuts at: 20 1769 3325 20
Size: 1749 1556 4401
Fragments arranged by size:
4401 1749 1556
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7706
AvaI C'yCGr_G
Cuts at: 4631 4726 4631
Size: 95 7611
AvrII C'CTAG_G
Cuts at: 3219 3219
Size: 7706
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2987 3020 2987
Size: 33 7673
BamHI G'GATC_C
Cuts at: 850 3360 4437 4731 850
Size: 2510 1077 294 3825
Fragments arranged by size:
3825 2510 1077 294
BanI G'GyrC_C
Cuts at: 1957 2479 2944 4399 4722 5136 5384 5419 1957
Size: 522 465 1455 323 414 248 35 4244
Fragments arranged by size:
4244 1455 522 465 414 323 248 35
BanII G_rGCy'C
Cuts at: 4720 5750 4720
Size: 1030 6676
BbeI G_GCGC'C
Cuts at: 5140 5388 5140
Size: 248 7458
BbsI GAAGACnn'nnnn_
Cuts at: 1645 1699 2130 2363 2959 3063 3620 4271 4366
Size: 54 431 233 596 104 557 651 95
Cuts at: 4366 4373 1645
Size: 7 4978
Fragments arranged by size:
4978 651 596 557 431 233 104 95
54 7
BciVI GTATCCnnnnn_n'
Cuts at: 2023 5604 6365 7706 2023
Size: 3581 761 1341 2023
Fragments arranged by size:
3581 2023 1341 761
BclI T'GATC_A
Cuts at: 1098 2574 5226 1098
Size: 1476 2652 3578
Fragments arranged by size:
3578 2652 1476
BglII A'GATC_T
Cuts at: 2245 5221 7258 2245
Size: 2976 2037 2693
Fragments arranged by size:
2976 2693 2037
BlpI GC'TnA_GC
Cuts at: 2536 4818 2536
Size: 2282 5424
Bme1580I G_kGCm'C
Cuts at: 1960 5331 5424 6480 1960
Size: 3371 93 1056 3186
Fragments arranged by size:
3371 3186 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1356 2690 4324 4918 4999 5334 7478 1356
Size: 1334 1634 594 81 335 2144 1584
Fragments arranged by size:
2144 1634 1584 1334 594 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1928 6073 1928
Size: 4145 3561
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1837 1837
Size: 7706
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 3836 4882 5761 6253 6551 709
Size: 3127 1046 879 492 298 1864
Fragments arranged by size:
3127 1864 1046 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 7410 7410
Size: 7706
BsaAI yAC'GTr
Cuts at: 3123 5059 3123
Size: 1936 5770
BsaBI GATnn'nnATC
Cuts at: 6 3359 5220 7257 6
Size: 3353 1861 2037 455
Fragments arranged by size:
3353 2037 1861 455
BsaHI Gr'CG_yC
Cuts at: 5137 5385 6087 7583 5137
Size: 248 702 1496 5260
Fragments arranged by size:
5260 1496 702 248
BsaWI w'CCGG_w
Cuts at: 438 4396 4895 5416 6094 6368 6515 6926 438
Size: 3958 499 521 678 274 147 411 1218
Fragments arranged by size:
3958 1218 678 521 499 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2951 4619 2951
Size: 1668 6038
BseYI C'CCAG_C
Cuts at: 281 519 3386 5131 5889 6466 6962 281
Size: 238 2867 1745 758 577 496 1025
Fragments arranged by size:
2867 1745 1025 758 577 496 238
BsiEI CG_ry'CG
Cuts at: 104 5248 5294 6502 104
Size: 5144 46 1208 1308
Fragments arranged by size:
5144 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 4720 5498 5688 6480 4720
Size: 778 190 792 5946
Fragments arranged by size:
5946 792 778 190
BsmI GAATG_Cn'
Cuts at: 3230 3230
Size: 7706
Bsp1286I G_dGCh'C
Cuts at: 1960 4720 5331 5424 5498 5688 5750 6480 1960
Size: 2760 611 93 74 190 62 730 3186
Fragments arranged by size:
3186 2760 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 438 6926 438
Size: 6488 1218
BspHI T'CATG_A
Cuts at: 6882 6882
Size: 7706
BspMI ACCTGCnnnn'nnnn_
Cuts at: 4756 4970 5272 5653 4756
Size: 214 302 381 6809
Fragments arranged by size:
6809 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5998 6052 7536 7701 50
Size: 5948 54 1484 165 55
Fragments arranged by size:
5948 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 121 1055 5618 121
Size: 934 4563 2209
Fragments arranged by size:
4563 2209 934
BsrFI r'CCGG_y
Cuts at: 4396 5704 5885 6094 4396
Size: 1308 181 209 6008
Fragments arranged by size:
6008 1308 209 181
BsrGI T'GTAC_A
Cuts at: 350 350
Size: 7706
BssSI C'ACGA_G
Cuts at: 4043 5977 6335 4043
Size: 1934 358 5414
Fragments arranged by size:
5414 1934 358
BstAPI GCAn_nnn'nTGC
Cuts at: 5083 5083
Size: 7706
BstBI TT'CG_AA
Cuts at: 4555 6067 4555
Size: 1512 6194
BstXI CCAn_nnnn'nTGG
Cuts at: 1509 2350 1509
Size: 841 6865
BstYI r'GATC_y
Cuts at: 1 850 2245 3360 4437 4731 5198 5221 5556
Size: 849 1395 1115 1077 294 467 23 335
Cuts at: 5556 5802 6814 6900 6912 7258 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1395 1115 1077 1012 849 467 449 346
335 294 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2150 2150
Size: 7706
BtgI C'CryG_G
Cuts at: 107 2645 4057 107
Size: 2538 1412 3756
Fragments arranged by size:
3756 2538 1412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5656 5837 6000 5656
Size: 181 163 7362
Fragments arranged by size:
7362 181 163
BtsI GCAGTG_nn'
Cuts at: 4020 4272 4949 4020
Size: 252 677 6777
Fragments arranged by size:
6777 677 252
DraI TTT'AAA
Cuts at: 786 3134 4705 786
Size: 2348 1571 3787
Fragments arranged by size:
3787 2348 1571
DraIII CAC_nnn'GTG
Cuts at: 1469 1469
Size: 7706
DrdI GACnn_nn'nnGTC
Cuts at: 5412 6270 5412
Size: 858 6848
EaeI y'GGCC_r
Cuts at: 5291 5465 5856 5883 7228 5291
Size: 174 391 27 1345 5769
Fragments arranged by size:
5769 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5291 5291
Size: 7706
EarI CTCTTCn'nnn_
Cuts at: 699 825 1802 1889 1897 3413 3970 4670 5729
Size: 126 977 87 8 1516 557 700 1059
Cuts at: 5729 5939 699
Size: 210 2466
Fragments arranged by size:
2466 1516 1059 977 700 557 210 126
87 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 232 5914 6224 6370 7516 232
Size: 5682 310 146 1146 422
Fragments arranged by size:
5682 1146 422 310 146
EcoICRI GAG'CTC
Cuts at: 4718 4718
Size: 7706
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1639 1837 2057 2687 3047 3752 3860 5531 5963
Size: 198 220 630 360 705 108 1671 432
Cuts at: 5963 6710 6978 1639
Size: 747 268 2367
Fragments arranged by size:
2367 1671 747 705 630 432 360 268
220 198 108
EcoNI CCTnn'n_nnAGG
Cuts at: 1486 1565 3692 1486
Size: 79 2127 5500
Fragments arranged by size:
5500 2127 79
EcoO109I rG'GnC_Cy
Cuts at: 402 4845 402
Size: 4443 3263
EcoRI G'AATT_C
Cuts at: 1418 4449 4710 1418
Size: 3031 261 4414
Fragments arranged by size:
4414 3031 261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 892 3700 4397 5725 892
Size: 2808 697 1328 2873
Fragments arranged by size:
2873 2808 1328 697
FspI TGC'GCA
Cuts at: 5487 5487
Size: 7706
HaeII r_GCGC'y
Cuts at: 5140 5388 6410 7389 5140
Size: 248 1022 979 5457
Fragments arranged by size:
5457 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 573 605 1635 1667 1997 2029 2070 2102 2946
Size: 32 1030 32 330 32 41 32 844
Cuts at: 2946 2978 3985 4017 4516 4548 4600 4632 5548
Size: 32 1007 32 499 32 52 32 916
Cuts at: 5548 5578 5580 5610 573
Size: 30 2 30 2669
Fragments arranged by size:
2669 1030 1007 916 844 499 330 52
41 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 153 1050 2102 4745 153
Size: 897 1052 2643 3114
Fragments arranged by size:
3114 2643 1052 897
HindIII A'AGCT_T
Cuts at: 1592 1592
Size: 7706
HpaI GTT'AAC
Cuts at: 2102 2102
Size: 7706
KasI G'GCGC_C
Cuts at: 5136 5384 5136
Size: 248 7458
KpnI G_GTAC'C
Cuts at: 2483 4726 2483
Size: 2243 5463
MfeI C'AATT_G
Cuts at: 223 431 1285 223
Size: 208 854 6644
Fragments arranged by size:
6644 854 208
MluI A'CGCG_T
Cuts at: 7139 7139
Size: 7706
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 923 3036 3134 3660 6377 6561 923
Size: 2113 98 526 2717 184 2068
Fragments arranged by size:
2717 2113 2068 526 184 98
MscI TGG'CCA
Cuts at: 5467 5467
Size: 7706
MslI CAynn'nnrTG
Cuts at: 112 2384 3784 5695 5822 112
Size: 2272 1400 1911 127 1996
Fragments arranged by size:
2272 1996 1911 1400 127
MspA1I CmG'CkG
Cuts at: 1393 4621 4817 5131 5491 6504 6749 1393
Size: 3228 196 314 360 1013 245 2350
Fragments arranged by size:
3228 2350 1013 360 314 245 196
NaeI GCC'GGC
Cuts at: 5887 5887
Size: 7706
NarI GG'CG_CC
Cuts at: 5137 5385 5137
Size: 248 7458
NcoI C'CATG_G
Cuts at: 107 2645 107
Size: 2538 5168
NdeI CA'TA_TG
Cuts at: 837 4604 837
Size: 3767 3939
NgoMIV G'CCGG_C
Cuts at: 5885 5885
Size: 7706
NspI r_CATG'y
Cuts at: 755 927 1084 1188 1283 3098 4759 5259 6166
Size: 172 157 104 95 1815 1661 500 907
Cuts at: 6166 755
Size: 2295
Fragments arranged by size:
2295 1815 1661 907 500 172 157 104
95
PacI TTA_AT'TAA
Cuts at: 3329 3329
Size: 7706
PciI A'CATG_T
Cuts at: 751 923 1080 1184 6162 751
Size: 172 157 104 4978 2295
Fragments arranged by size:
4978 2295 172 157 104
PmeI GTTT'AAAC
Cuts at: 4705 4705
Size: 7706
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6845 6877 6845
Size: 32 7674
PpuMI rG'GwC_Cy
Cuts at: 402 402
Size: 7706
PstI C_TGCA'G
Cuts at: 3544 4753 5438 3544
Size: 1209 685 5812
Fragments arranged by size:
5812 1209 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7706
PvuII CAG'CTG
Cuts at: 1393 4621 5131 5491 1393
Size: 3228 510 360 3608
Fragments arranged by size:
3608 3228 510 360
RsrII CG'GwC_CG
Cuts at: 5901 5901
Size: 7706
SacI G_AGCT'C
Cuts at: 4720 4720
Size: 7706
SalI G'TCGA_C
Cuts at: 4743 4743
Size: 7706
SanDI GG'GwC_CC
Cuts at: 402 402
Size: 7706
SapI GCTCTTCn'nnn_
Cuts at: 699 825 5729 5939 699
Size: 126 4904 210 2466
Fragments arranged by size:
4904 2466 210 126
SbfI CC_TGCA'GG
Cuts at: 4753 4753
Size: 7706
ScaI AGT'ACT
Cuts at: 7223 7223
Size: 7706
SexAI A'CCwGG_T
Cuts at: 2277 3915 2277
Size: 1638 6068
SfcI C'TryA_G
Cuts at: 33 321 2976 3540 4749 5434 6427 6618 33
Size: 288 2655 564 1209 685 993 191 1121
Fragments arranged by size:
2655 1209 1121 993 685 564 288 191
SfoI GGC'GCC
Cuts at: 5138 5386 5138
Size: 248 7458
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7706
SmaI CCC'GGG
Cuts at: 4728 4728
Size: 7706
SmlI C'TyrA_G
Cuts at: 724 3851 4861 5776 6268 6530 724
Size: 3127 1010 915 492 262 1900
Fragments arranged by size:
3127 1900 1010 915 492 262
SpeI A'CTAG_T
Cuts at: 67 3085 67
Size: 3018 4688
SphI G_CATG'C
Cuts at: 4759 5259 4759
Size: 500 7206
SspI AAT'ATT
Cuts at: 1942 2026 3193 1942
Size: 84 1167 6455
Fragments arranged by size:
6455 1167 84
StuI AGG'CCT
Cuts at: 1523 3373 3878 1523
Size: 1850 505 5351
Fragments arranged by size:
5351 1850 505
StyI C'CwwG_G
Cuts at: 107 1067 1524 2645 3219 3518 3656 3707 4580
Size: 960 457 1121 574 299 138 51 873
Cuts at: 4580 4840 5194 107
Size: 260 354 2619
Fragments arranged by size:
2619 1121 960 873 574 457 354 299
260 138 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1338 2672 5289 5422 5881 6090 1338
Size: 1334 2617 133 459 209 2954
Fragments arranged by size:
2954 2617 1334 459 209 133
TatI w'GTAC_w
Cuts at: 350 1658 7221 350
Size: 1308 5563 835
Fragments arranged by size:
5563 1308 835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 324 356 420 452 7223 7255 324
Size: 32 64 32 6771 32 775
Fragments arranged by size:
6771 775 64 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 5503 5503
Size: 7706
XbaI T'CTAG_A
Cuts at: 2925 4737 2925
Size: 1812 5894
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 639 2415 3602 5201 639
Size: 1776 1187 1599 3144
Fragments arranged by size:
3144 1776 1599 1187
XmaI C'CCGG_G
Cuts at: 4726 4726
Size: 7706
XmnI GAAnn'nnTTC
Cuts at: 673 2921 673
Size: 2248 5458
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PacI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmBI BssHII BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI NheI NotI NruI NsiI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SwaI XhoI
ZraI