Restriction map for
pF1KE0791
(Circular) (Six-base) MAPSORT of: pF1KE0791.seq Check: 7171 from: 1 to: 4184
pF1KE0791 4184 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AccI GT'mk_AC
Cuts at: 1222 1222
Size: 4184
Acc65I G'GTAC_C
Cuts at: 1200 1200
Size: 4184
AclI AA'CG_TT
Cuts at: 4024 4024
Size: 4184
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 513 2009 2441 3188 3456 411
Size: 102 1496 432 747 268 1139
Fragments arranged by size:
1496 1139 747 432 268 102
AflIII A'CryG_T
Cuts at: 930 1536 2640 3617 930
Size: 606 1104 977 1497
Fragments arranged by size:
1497 1104 977 606
AgeI A'CCGG_T
Cuts at: 2572 2572
Size: 4184
AleI CACnn'nnGTG
Cuts at: 806 806
Size: 4184
AlwNI CAG_nnn'CTG
Cuts at: 1489 3056 1489
Size: 1567 2617
ApaLI G'TGCA_C
Cuts at: 2954 2954
Size: 4184
ApoI r'AATT_y
Cuts at: 642 835 1188 3537 3793 642
Size: 193 353 2349 256 1033
Fragments arranged by size:
2349 1033 353 256 193
AseI AT'TA_AT
Cuts at: 20 1159 20
Size: 1139 3045
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
AvaI C'yCGr_G
Cuts at: 516 1204 516
Size: 688 3496
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 719 752 719
Size: 33 4151
BamHI G'GATC_C
Cuts at: 1209 1209
Size: 4184
BanI G'GyrC_C
Cuts at: 1200 1614 1862 1897 1200
Size: 414 248 35 3487
Fragments arranged by size:
3487 414 248 35
BanII G_rGCy'C
Cuts at: 944 1198 2228 944
Size: 254 1030 2900
Fragments arranged by size:
2900 1030 254
BbeI G_GCGC'C
Cuts at: 1618 1866 1618
Size: 248 3936
BbsI GAAGACnn'nnnn_
Cuts at: 163 373 163
Size: 210 3974
BciVI GTATCCnnnnn_n'
Cuts at: 2082 2843 4184 2082
Size: 761 1341 2082
Fragments arranged by size:
2082 1341 761
BclI T'GATC_A
Cuts at: 192 1031 1704 192
Size: 839 673 2672
Fragments arranged by size:
2672 839 673
BglII A'GATC_T
Cuts at: 422 1699 3736 422
Size: 1277 2037 870
Fragments arranged by size:
2037 1277 870
BlpI GC'TnA_GC
Cuts at: 1296 1296
Size: 4184
Bme1580I G_kGCm'C
Cuts at: 1809 1902 2958 1809
Size: 93 1056 3035
Fragments arranged by size:
3035 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1396 1477 1812 3956 1396
Size: 81 335 2144 1624
Fragments arranged by size:
2144 1624 335 81
BmtI G_CTAG'C
Cuts at: 361 610 361
Size: 249 3935
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2551 2551
Size: 4184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1360 2239 2731 3029 1360
Size: 879 492 298 2515
Fragments arranged by size:
2515 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3888 3888
Size: 4184
BsaAI yAC'GTr
Cuts at: 1537 1537
Size: 4184
BsaBI GATnn'nnATC
Cuts at: 6 1698 3735 6
Size: 1692 2037 455
Fragments arranged by size:
2037 1692 455
BsaHI Gr'CG_yC
Cuts at: 1615 1863 2565 4061 1615
Size: 248 702 1496 1738
Fragments arranged by size:
1738 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1373 1894 2572 2846 2993 3404 1373
Size: 521 678 274 147 411 2153
Fragments arranged by size:
2153 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 649 679 649
Size: 30 4154
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 670 670
Size: 4184
BseYI C'CCAG_C
Cuts at: 1609 2367 2944 3440 1609
Size: 758 577 496 2353
Fragments arranged by size:
2353 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1726 1772 2980 104
Size: 1622 46 1208 1308
Fragments arranged by size:
1622 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 944 1198 1976 2166 2958 944
Size: 254 778 190 792 2170
Fragments arranged by size:
2170 792 778 254 190
BsmI GAATG_Cn'
Cuts at: 896 896
Size: 4184
Bsp1286I G_dGCh'C
Cuts at: 944 1198 1809 1902 1976 2166 2228 2958 944
Size: 254 611 93 74 190 62 730 2170
Fragments arranged by size:
2170 730 611 254 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3404 3404
Size: 4184
BspHI T'CATG_A
Cuts at: 1151 3360 1151
Size: 2209 1975
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1234 1448 1750 2131 1234
Size: 214 302 381 3287
Fragments arranged by size:
3287 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2476 2530 4014 4179 50
Size: 2426 54 1484 165 55
Fragments arranged by size:
2426 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2096 2096
Size: 4184
BsrFI r'CCGG_y
Cuts at: 2182 2363 2572 2182
Size: 181 209 3794
Fragments arranged by size:
3794 209 181
BssSI C'ACGA_G
Cuts at: 2455 2813 2455
Size: 358 3826
BstAPI GCAn_nnn'nTGC
Cuts at: 1561 1561
Size: 4184
BstBI TT'CG_AA
Cuts at: 2545 2545
Size: 4184
BstYI r'GATC_y
Cuts at: 1 422 811 1114 1209 1676 1699 2034 2280
Size: 421 389 303 95 467 23 335 246
Cuts at: 2280 3292 3378 3390 3736 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 421 389 346 335 303
246 95 86 23 12
BtgI C'CryG_G
Cuts at: 107 560 107
Size: 453 3731
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2134 2315 2478 2134
Size: 181 163 3840
Fragments arranged by size:
3840 181 163
BtsI GCAGTG_nn'
Cuts at: 1427 1427
Size: 4184
DraI TTT'AAA
Cuts at: 1140 1183 1140
Size: 43 4141
DrdI GACnn_nn'nnGTC
Cuts at: 1890 2748 1890
Size: 858 3326
EaeI y'GGCC_r
Cuts at: 203 1769 1943 2334 2361 3706 203
Size: 1566 174 391 27 1345 681
Fragments arranged by size:
1566 1345 681 391 174 27
EagI C'GGCC_G
Cuts at: 1769 1769
Size: 4184
EarI CTCTTCn'nnn_
Cuts at: 211 2207 2417 211
Size: 1996 210 1978
Fragments arranged by size:
1996 1978 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 263 2392 2702 2848 3994 263
Size: 2129 310 146 1146 453
Fragments arranged by size:
2129 1146 453 310 146
EcoICRI GAG'CTC
Cuts at: 942 1196 942
Size: 254 3930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 513 2009 2441 3188 3456 411
Size: 102 1496 432 747 268 1139
Fragments arranged by size:
1496 1139 747 432 268 102
EcoO109I rG'GnC_Cy
Cuts at: 728 1323 728
Size: 595 3589
EcoRI G'AATT_C
Cuts at: 1188 1188
Size: 4184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 240 2203 240
Size: 1963 2221
FspI TGC'GCA
Cuts at: 1965 1965
Size: 4184
HaeII r_GCGC'y
Cuts at: 1618 1866 2888 3867 1618
Size: 248 1022 979 1935
Fragments arranged by size:
1935 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 940 972 2026 2056 2058 2088 940
Size: 32 1054 30 2 30 3036
Fragments arranged by size:
3036 1054 32 30 30 2
HincII GTy'rAC
Cuts at: 180 1223 180
Size: 1043 3141
HindIII A'AGCT_T
Cuts at: 525 976 525
Size: 451 3733
KasI G'GCGC_C
Cuts at: 1614 1862 1614
Size: 248 3936
KpnI G_GTAC'C
Cuts at: 1204 1204
Size: 4184
MluI A'CGCG_T
Cuts at: 3617 3617
Size: 4184
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 298 2855 3039 298
Size: 2557 184 1443
Fragments arranged by size:
2557 1443 184
MscI TGG'CCA
Cuts at: 1945 1945
Size: 4184
MslI CAynn'nnrTG
Cuts at: 413 806 2173 2300 413
Size: 393 1367 127 2297
Fragments arranged by size:
2297 1367 393 127
MspA1I CmG'CkG
Cuts at: 1295 1609 1969 2982 3227 1295
Size: 314 360 1013 245 2252
Fragments arranged by size:
2252 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2365 2365
Size: 4184
NarI GG'CG_CC
Cuts at: 1615 1863 1615
Size: 248 3936
NcoI C'CATG_G
Cuts at: 107 107
Size: 4184
NdeI CA'TA_TG
Cuts at: 297 297
Size: 4184
NgoMIV G'CCGG_C
Cuts at: 2363 2363
Size: 4184
NheI G'CTAG_C
Cuts at: 357 606 357
Size: 249 3935
NspI r_CATG'y
Cuts at: 379 620 934 964 1046 1237 1737 2644 379
Size: 241 314 30 82 191 500 907 1919
Fragments arranged by size:
1919 907 500 314 241 191 82 30
PciI A'CATG_T
Cuts at: 930 2640 930
Size: 1710 2474
PmeI GTTT'AAAC
Cuts at: 1183 1183
Size: 4184
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3323 3355 3323
Size: 32 4152
PpuMI rG'GwC_Cy
Cuts at: 728 728
Size: 4184
PstI C_TGCA'G
Cuts at: 1231 1916 1231
Size: 685 3499
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4184
PvuII CAG'CTG
Cuts at: 1609 1969 1609
Size: 360 3824
RsrII CG'GwC_CG
Cuts at: 2379 2379
Size: 4184
SacI G_AGCT'C
Cuts at: 944 1198 944
Size: 254 3930
SalI G'TCGA_C
Cuts at: 1221 1221
Size: 4184
SapI GCTCTTCn'nnn_
Cuts at: 211 2207 2417 211
Size: 1996 210 1978
Fragments arranged by size:
1996 1978 210
SbfI CC_TGCA'GG
Cuts at: 1231 1231
Size: 4184
ScaI AGT'ACT
Cuts at: 3701 3701
Size: 4184
SfcI C'TryA_G
Cuts at: 33 694 1227 1912 2905 3096 33
Size: 661 533 685 993 191 1121
Fragments arranged by size:
1121 993 685 661 533 191
SfoI GGC'GCC
Cuts at: 1616 1864 1616
Size: 248 3936
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4184
SmaI CCC'GGG
Cuts at: 1206 1206
Size: 4184
SmlI C'TyrA_G
Cuts at: 1339 2254 2746 3008 1339
Size: 915 492 262 2515
Fragments arranged by size:
2515 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4184
SphI G_CATG'C
Cuts at: 1237 1737 1237
Size: 500 3684
SspI AAT'ATT
Cuts at: 305 305
Size: 4184
StyI C'CwwG_G
Cuts at: 107 284 1318 1672 107
Size: 177 1034 354 2619
Fragments arranged by size:
2619 1034 354 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1767 1900 2359 2568 1767
Size: 133 459 209 3383
Fragments arranged by size:
3383 459 209 133
TatI w'GTAC_w
Cuts at: 3699 3699
Size: 4184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 649 681 3701 3733 649
Size: 32 3020 32 1100
Fragments arranged by size:
3020 1100 32 32
Tth111I GACn'n_nGTC
Cuts at: 1981 1981
Size: 4184
XbaI T'CTAG_A
Cuts at: 480 1215 480
Size: 735 3449
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1679 1679
Size: 4184
XmaI C'CCGG_G
Cuts at: 1204 1204
Size: 4184
XmnI GAAnn'nnTTC
Cuts at: 835 835
Size: 4184
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NspI PciI PmeI PpiI PpuMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BbvCI BcgI BfrBI BglI BmgBI BpmI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI