Restriction map for
pF1KE0785
(Circular) (Six-base) MAPSORT of: pF1KE0785.seq Check: 6667 from: 1 to: 4172
pF1KE0785 4172 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 201 726 201
Size: 525 3647
AccI GT'mk_AC
Cuts at: 1210 1210
Size: 4172
Acc65I G'GTAC_C
Cuts at: 974 1150 1188 974
Size: 176 38 3958
Fragments arranged by size:
3958 176 38
AclI AA'CG_TT
Cuts at: 4012 4012
Size: 4172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 369 787 993 1997 2429 3176 3444 369
Size: 418 206 1004 432 747 268 1097
Fragments arranged by size:
1097 1004 747 432 418 268 206
AflIII A'CryG_T
Cuts at: 311 1524 2628 3605 311
Size: 1213 1104 977 878
Fragments arranged by size:
1213 1104 977 878
AgeI A'CCGG_T
Cuts at: 2560 2560
Size: 4172
AleI CACnn'nnGTG
Cuts at: 725 725
Size: 4172
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 583 583
Size: 4172
AlwNI CAG_nnn'CTG
Cuts at: 588 891 1477 3044 588
Size: 303 586 1567 1716
Fragments arranged by size:
1716 1567 586 303
ApaLI G'TGCA_C
Cuts at: 2942 2942
Size: 4172
ApoI r'AATT_y
Cuts at: 661 1176 3525 3781 661
Size: 515 2349 256 1052
Fragments arranged by size:
2349 1052 515 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4172
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4172
AvaI C'yCGr_G
Cuts at: 426 640 872 1192 426
Size: 214 232 320 3406
Fragments arranged by size:
3406 320 232 214
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1142 1175 1142
Size: 33 4139
BamHI G'GATC_C
Cuts at: 1197 1197
Size: 4172
BanI G'GyrC_C
Cuts at: 636 962 974 1002 1150 1188 1602 1850 1885
Size: 326 12 28 148 38 414 248 35
Cuts at: 1885 636
Size: 2923
Fragments arranged by size:
2923 414 326 248 148 38 35 28
12
BanII G_rGCy'C
Cuts at: 873 1186 2216 873
Size: 313 1030 2829
Fragments arranged by size:
2829 1030 313
BbeI G_GCGC'C
Cuts at: 640 1606 1854 640
Size: 966 248 2958
Fragments arranged by size:
2958 966 248
BbvCI CC'TCA_GC
Cuts at: 1142 1142
Size: 4172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 760 794 90
Size: 34 636 34 3468
Fragments arranged by size:
3468 636 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2070 2831 4172 2070
Size: 761 1341 2070
Fragments arranged by size:
2070 1341 761
BclI T'GATC_A
Cuts at: 1692 1692
Size: 4172
BglI GCCn_nnn'nGGC
Cuts at: 697 697
Size: 4172
BglII A'GATC_T
Cuts at: 1687 3724 1687
Size: 2037 2135
BlpI GC'TnA_GC
Cuts at: 526 1284 526
Size: 758 3414
Bme1580I G_kGCm'C
Cuts at: 1007 1797 1890 2946 1007
Size: 790 93 1056 2233
Fragments arranged by size:
2233 1056 790 93
BmgBI CAC'GTC
Cuts at: 312 312
Size: 4172
BmrI ACTGGGnnnn_n'
Cuts at: 1384 1465 1800 3944 1384
Size: 81 335 2144 1612
Fragments arranged by size:
2144 1612 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2539 2539
Size: 4172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 193
Size: 4172
Bpu10I CC'TnA_GC
Cuts at: 1142 1142
Size: 4172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 1348 2227 2719 3017 739
Size: 609 879 492 298 1894
Fragments arranged by size:
1894 879 609 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3876 3876
Size: 4172
BsaAI yAC'GTr
Cuts at: 1525 1525
Size: 4172
BsaBI GATnn'nnATC
Cuts at: 6 1686 3723 6
Size: 1680 2037 455
Fragments arranged by size:
2037 1680 455
BsaHI Gr'CG_yC
Cuts at: 637 1603 1851 2553 4049 637
Size: 966 248 702 1496 760
Fragments arranged by size:
1496 966 760 702 248
BsaWI w'CCGG_w
Cuts at: 999 1361 1882 2560 2834 2981 3392 999
Size: 362 521 678 274 147 411 1779
Fragments arranged by size:
1779 678 521 411 362 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 722 752 722
Size: 30 4142
BseYI C'CCAG_C
Cuts at: 251 274 588 622 909 1135 1597 2355 2932
Size: 23 314 34 287 226 462 758 577
Cuts at: 2932 3428 251
Size: 496 995
Fragments arranged by size:
995 758 577 496 462 314 287 226
34 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 229 445 229
Size: 216 3956
BsiEI CG_ry'CG
Cuts at: 104 481 1714 1760 2968 104
Size: 377 1233 46 1208 1308
Fragments arranged by size:
1308 1233 1208 377 46
BsiHKAI G_wGCw'C
Cuts at: 323 1186 1964 2154 2946 323
Size: 863 778 190 792 1549
Fragments arranged by size:
1549 863 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 323 873 1007 1186 1797 1890 1964 2154 2216
Size: 550 134 179 611 93 74 190 62
Cuts at: 2216 2946 323
Size: 730 1549
Fragments arranged by size:
1549 730 611 550 190 179 134 93
74 62
BspEI T'CCGG_A
Cuts at: 3392 3392
Size: 4172
BspHI T'CATG_A
Cuts at: 3348 3348
Size: 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 201 726 960 1138 1222 1436 1738 2119 201
Size: 525 234 178 84 214 302 381 2254
Fragments arranged by size:
2254 525 381 302 234 214 178 84
BsrBI CCG'CTC
Cuts at: 50 2464 2518 4002 4167 50
Size: 2414 54 1484 165 55
Fragments arranged by size:
2414 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2084 2084
Size: 4172
BsrFI r'CCGG_y
Cuts at: 633 2170 2351 2560 633
Size: 1537 181 209 2245
Fragments arranged by size:
2245 1537 209 181
BssHII G'CGCG_C
Cuts at: 437 563 437
Size: 126 4046
BssSI C'ACGA_G
Cuts at: 496 2443 2801 496
Size: 1947 358 1867
Fragments arranged by size:
1947 1867 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1002 1549 1002
Size: 547 3625
BstBI TT'CG_AA
Cuts at: 2533 2533
Size: 4172
BstXI CCAn_nnnn'nTGG
Cuts at: 939 939
Size: 4172
BstYI r'GATC_y
Cuts at: 1 1197 1664 1687 2022 2268 3280 3366 3378
Size: 1196 467 23 335 246 1012 86 12
Cuts at: 3378 3724 1
Size: 346 449
Fragments arranged by size:
1196 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 305 305
Size: 4172
BtgI C'CryG_G
Cuts at: 399 686 1154 399
Size: 287 468 3417
Fragments arranged by size:
3417 468 287
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 435 2122 2303 2466 435
Size: 1687 181 163 2141
Fragments arranged by size:
2141 1687 181 163
BtsI GCAGTG_nn'
Cuts at: 1415 1415
Size: 4172
DraI TTT'AAA
Cuts at: 1171 1171
Size: 4172
DraIII CAC_nnn'GTG
Cuts at: 935 1105 935
Size: 170 4002
DrdI GACnn_nn'nnGTC
Cuts at: 1878 2736 1878
Size: 858 3314
EaeI y'GGCC_r
Cuts at: 478 689 1044 1064 1757 1931 2322 2349 3694
Size: 211 355 20 693 174 391 27 1345
Cuts at: 3694 478
Size: 956
Fragments arranged by size:
1345 956 693 391 355 211 174 27
20
EagI C'GGCC_G
Cuts at: 478 1757 478
Size: 1279 2893
EarI CTCTTCn'nnn_
Cuts at: 2195 2405 2195
Size: 210 3962
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 190 358 2380 2690 2836 3982 190
Size: 168 2022 310 146 1146 380
Fragments arranged by size:
2022 1146 380 310 168 146
EcoICRI GAG'CTC
Cuts at: 1184 1184
Size: 4172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 369 787 993 1997 2429 3176 3444 193
Size: 176 418 206 1004 432 747 268 921
Fragments arranged by size:
1004 921 747 432 418 268 206 176
EcoNI CCTnn'n_nnAGG
Cuts at: 208 208
Size: 4172
EcoO109I rG'GnC_Cy
Cuts at: 592 699 796 949 1311 592
Size: 107 97 153 362 3453
Fragments arranged by size:
3453 362 153 107 97
EcoRI G'AATT_C
Cuts at: 1176 1176
Size: 4172
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2191 2191
Size: 4172
FspI TGC'GCA
Cuts at: 1953 1953
Size: 4172
HaeII r_GCGC'y
Cuts at: 172 640 1606 1854 2876 3855 172
Size: 468 966 248 1022 979 489
Fragments arranged by size:
1022 979 966 489 468 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 135 167 1063 1095 2014 2044 2046 2076 135
Size: 32 896 32 919 30 2 30 2231
Fragments arranged by size:
2231 919 896 32 32 30 30 2
HincII GTy'rAC
Cuts at: 534 1211 534
Size: 677 3495
KasI G'GCGC_C
Cuts at: 636 1602 1850 636
Size: 966 248 2958
Fragments arranged by size:
2958 966 248
KpnI G_GTAC'C
Cuts at: 978 1154 1192 978
Size: 176 38 3958
Fragments arranged by size:
3958 176 38
MluI A'CGCG_T
Cuts at: 3605 3605
Size: 4172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2843 3027 2843
Size: 184 3988
MscI TGG'CCA
Cuts at: 691 1066 1933 691
Size: 375 867 2930
Fragments arranged by size:
2930 867 375
MslI CAynn'nnrTG
Cuts at: 725 2161 2288 725
Size: 1436 127 2609
Fragments arranged by size:
2609 1436 127
MspA1I CmG'CkG
Cuts at: 274 336 389 401 474 492 588 682 1013
Size: 62 53 12 73 18 96 94 331
Cuts at: 1013 1283 1597 1957 2970 3215 274
Size: 270 314 360 1013 245 1231
Fragments arranged by size:
1231 1013 360 331 314 270 245 96
94 73 62 53 18 12
NaeI GCC'GGC
Cuts at: 2353 2353
Size: 4172
NarI GG'CG_CC
Cuts at: 637 1603 1851 637
Size: 966 248 2958
Fragments arranged by size:
2958 966 248
NcoI C'CATG_G
Cuts at: 686 1154 686
Size: 468 3704
NgoMIV G'CCGG_C
Cuts at: 2351 2351
Size: 4172
NspI r_CATG'y
Cuts at: 1225 1725 2632 1225
Size: 500 907 2765
Fragments arranged by size:
2765 907 500
PciI A'CATG_T
Cuts at: 2628 2628
Size: 4172
PflMI CCAn_nnn'nTGG
Cuts at: 348 348
Size: 4172
PmeI GTTT'AAAC
Cuts at: 1171 1171
Size: 4172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3311 3343 3311
Size: 32 4140
PpuMI rG'GwC_Cy
Cuts at: 592 796 949 592
Size: 204 153 3815
Fragments arranged by size:
3815 204 153
PspXI vC'TCGA_Gb
Cuts at: 426 426
Size: 4172
PstI C_TGCA'G
Cuts at: 584 887 1097 1219 1904 584
Size: 303 210 122 685 2852
Fragments arranged by size:
2852 685 303 210 122
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4172
PvuII CAG'CTG
Cuts at: 274 1597 1957 274
Size: 1323 360 2489
Fragments arranged by size:
2489 1323 360
RsrII CG'GwC_CG
Cuts at: 631 2367 631
Size: 1736 2436
SacI G_AGCT'C
Cuts at: 1186 1186
Size: 4172
SacII CC_GC'GG
Cuts at: 402 402
Size: 4172
SalI G'TCGA_C
Cuts at: 1209 1209
Size: 4172
SanDI GG'GwC_CC
Cuts at: 592 796 592
Size: 204 3968
SapI GCTCTTCn'nnn_
Cuts at: 2195 2405 2195
Size: 210 3962
SbfI CC_TGCA'GG
Cuts at: 1219 1219
Size: 4172
ScaI AGT'ACT
Cuts at: 3689 3689
Size: 4172
SexAI A'CCwGG_T
Cuts at: 977 977
Size: 4172
SfcI C'TryA_G
Cuts at: 33 420 580 883 1093 1215 1900 2893 3084
Size: 387 160 303 210 122 685 993 191
Cuts at: 3084 33
Size: 1121
Fragments arranged by size:
1121 993 685 387 303 210 191 160
122
SfiI GGCCn_nnn'nGGCC
Cuts at: 697 697
Size: 4172
SfoI GGC'GCC
Cuts at: 638 1604 1852 638
Size: 966 248 2958
Fragments arranged by size:
2958 966 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4172
SmaI CCC'GGG
Cuts at: 642 1194 642
Size: 552 3620
SmlI C'TyrA_G
Cuts at: 426 754 1327 2242 2734 2996 426
Size: 328 573 915 492 262 1602
Fragments arranged by size:
1602 915 573 492 328 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4172
SphI G_CATG'C
Cuts at: 1225 1725 1225
Size: 500 3672
SrfI GCCC'GGGC
Cuts at: 642 642
Size: 4172
StyI C'CwwG_G
Cuts at: 686 864 1067 1154 1306 1660 686
Size: 178 203 87 152 354 3198
Fragments arranged by size:
3198 354 203 178 152 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 243 1755 1888 2347 2556 243
Size: 1512 133 459 209 1859
Fragments arranged by size:
1859 1512 459 209 133
TatI w'GTAC_w
Cuts at: 3687 3687
Size: 4172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 722 754 3689 3721 722
Size: 32 2935 32 1173
Fragments arranged by size:
2935 1173 32 32
Tth111I GACn'n_nGTC
Cuts at: 1969 1969
Size: 4172
XbaI T'CTAG_A
Cuts at: 1203 1203
Size: 4172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 248 699 1667 248
Size: 451 968 2753
Fragments arranged by size:
2753 968 451
XhoI C'TCGA_G
Cuts at: 426 426
Size: 4172
XmaI C'CCGG_G
Cuts at: 640 1192 640
Size: 552 3620
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PflMI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BbsI BfrBI BmtI BseRI BsiWI BsmI BsmBI BsrGI
BstEII BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PfoI PmlI PshAI PsiI PspOMI PsrI SgrAI
SnaBI SspI StuI SwaI XmnI ZraI