Restriction map for
pF1KE0768
(Circular) (Six-base) MAPSORT of: pF1KE0768.seq Check: 2903 from: 1 to: 4427
pF1KE0768 4427 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1058 1370 1058
Size: 312 4115
AccI GT'mk_AC
Cuts at: 1465 1465
Size: 4427
Acc65I G'GTAC_C
Cuts at: 1443 1443
Size: 4427
AclI AA'CG_TT
Cuts at: 4267 4267
Size: 4427
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 764 820 2252 2684 3431 3699 307
Size: 457 56 1432 432 747 268 1035
Fragments arranged by size:
1432 1035 747 457 432 268 56
AfeI AGC'GCT
Cuts at: 173 173
Size: 4427
AflIII A'CryG_T
Cuts at: 1223 1779 2883 3860 1223
Size: 556 1104 977 1790
Fragments arranged by size:
1790 1104 977 556
AgeI A'CCGG_T
Cuts at: 2815 2815
Size: 4427
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 599 631 599
Size: 32 4395
AlwNI CAG_nnn'CTG
Cuts at: 429 1732 3299 429
Size: 1303 1567 1557
Fragments arranged by size:
1567 1557 1303
ApaI G_GGCC'C
Cuts at: 827 827
Size: 4427
ApaLI G'TGCA_C
Cuts at: 1227 3197 1227
Size: 1970 2457
ApoI r'AATT_y
Cuts at: 1089 1431 3780 4036 1089
Size: 342 2349 256 1480
Fragments arranged by size:
2349 1480 342 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4427
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4427
AvaI C'yCGr_G
Cuts at: 1447 1447
Size: 4427
BamHI G'GATC_C
Cuts at: 1452 1452
Size: 4427
BanI G'GyrC_C
Cuts at: 1034 1259 1358 1443 1857 2105 2140 1034
Size: 225 99 85 414 248 35 3321
Fragments arranged by size:
3321 414 248 225 99 85 35
BanII G_rGCy'C
Cuts at: 224 827 1177 1441 2471 224
Size: 603 350 264 1030 2180
Fragments arranged by size:
2180 1030 603 350 264
BbeI G_GCGC'C
Cuts at: 1861 2109 1861
Size: 248 4179
BbsI GAAGACnn'nnnn_
Cuts at: 1009 1023 1009
Size: 14 4413
BbvCI CC'TCA_GC
Cuts at: 116 116
Size: 4427
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4393
BciVI GTATCCnnnnn_n'
Cuts at: 687 2325 3086 4427 687
Size: 1638 761 1341 687
Fragments arranged by size:
1638 1341 761 687
BclI T'GATC_A
Cuts at: 1947 1947
Size: 4427
BglII A'GATC_T
Cuts at: 1942 3979 1942
Size: 2037 2390
BlpI GC'TnA_GC
Cuts at: 1539 1539
Size: 4427
Bme1580I G_kGCm'C
Cuts at: 827 1203 1231 1315 2052 2145 3201 827
Size: 376 28 84 737 93 1056 2053
Fragments arranged by size:
2053 1056 737 376 93 84 28
BmrI ACTGGGnnnn_n'
Cuts at: 396 903 977 1301 1639 1720 2055 4199 396
Size: 507 74 324 338 81 335 2144 624
Fragments arranged by size:
2144 624 507 338 335 324 81 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 832 2794 832
Size: 1962 2465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 401 789 301
Size: 100 388 3939
Fragments arranged by size:
3939 388 100
Bpu10I CC'TnA_GC
Cuts at: 116 116
Size: 4427
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 976 1603 2482 2974 3272 976
Size: 627 879 492 298 2131
Fragments arranged by size:
2131 879 627 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 969 4131 969
Size: 3162 1265
BsaAI yAC'GTr
Cuts at: 1224 1780 1224
Size: 556 3871
BsaBI GATnn'nnATC
Cuts at: 6 189 1941 3978 6
Size: 183 1752 2037 455
Fragments arranged by size:
2037 1752 455 183
BsaHI Gr'CG_yC
Cuts at: 1858 2106 2808 4304 1858
Size: 248 702 1496 1981
Fragments arranged by size:
1981 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1616 2137 2815 3089 3236 3647 1616
Size: 521 678 274 147 411 2396
Fragments arranged by size:
2396 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 274 301 304 331 503 533 274
Size: 27 3 27 172 30 4168
Fragments arranged by size:
4168 172 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 322 484 614 652 322
Size: 162 130 38 4097
Fragments arranged by size:
4097 162 130 38
BseYI C'CCAG_C
Cuts at: 174 782 796 1397 1852 2610 3187 3683 174
Size: 608 14 601 455 758 577 496 918
Fragments arranged by size:
918 758 608 601 577 496 455 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 386 386
Size: 4427
BsiEI CG_ry'CG
Cuts at: 104 1969 2015 3223 104
Size: 1865 46 1208 1308
Fragments arranged by size:
1865 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 287 1075 1231 1441 2219 2409 3201 287
Size: 788 156 210 778 190 792 1513
Fragments arranged by size:
1513 792 788 778 210 190 156
BsmI GAATG_Cn'
Cuts at: 818 818
Size: 4427
Bsp1286I G_dGCh'C
Cuts at: 224 287 827 1075 1177 1203 1231 1315 1441
Size: 63 540 248 102 26 28 84 126
Cuts at: 1441 2052 2145 2219 2409 2471 3201 224
Size: 611 93 74 190 62 730 1450
Fragments arranged by size:
1450 730 611 540 248 190 126 102
93 84 74 63 62 28 26
BspEI T'CCGG_A
Cuts at: 3647 3647
Size: 4427
BspHI T'CATG_A
Cuts at: 3603 3603
Size: 4427
BspMI ACCTGCnnnn'nnnn_
Cuts at: 866 1058 1370 1477 1691 1993 2374 866
Size: 192 312 107 214 302 381 2919
Fragments arranged by size:
2919 381 312 302 214 192 107
BsrBI CCG'CTC
Cuts at: 50 252 2719 2773 4257 4422 50
Size: 202 2467 54 1484 165 55
Fragments arranged by size:
2467 1484 202 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2339 2339
Size: 4427
BsrFI r'CCGG_y
Cuts at: 2425 2606 2815 2425
Size: 181 209 4037
Fragments arranged by size:
4037 209 181
BsrGI T'GTAC_A
Cuts at: 201 201
Size: 4427
BssSI C'ACGA_G
Cuts at: 2698 3056 2698
Size: 358 4069
BstAPI GCAn_nnn'nTGC
Cuts at: 409 1148 1804 409
Size: 739 656 3032
Fragments arranged by size:
3032 739 656
BstBI TT'CG_AA
Cuts at: 2788 2788
Size: 4427
BstEII G'GTnAC_C
Cuts at: 432 432
Size: 4427
BstXI CCAn_nnnn'nTGG
Cuts at: 1165 1165
Size: 4427
BstYI r'GATC_y
Cuts at: 1 1452 1919 1942 2277 2523 3535 3621 3633
Size: 1451 467 23 335 246 1012 86 12
Cuts at: 3633 3979 1
Size: 346 449
Fragments arranged by size:
1451 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 256 256
Size: 4427
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2377 2558 2721 2377
Size: 181 163 4083
Fragments arranged by size:
4083 181 163
BtsI GCAGTG_nn'
Cuts at: 1670 1670
Size: 4427
DraI TTT'AAA
Cuts at: 1426 1426
Size: 4427
DraIII CAC_nnn'GTG
Cuts at: 1269 1269
Size: 4427
DrdI GACnn_nn'nnGTC
Cuts at: 2133 2991 2133
Size: 858 3569
EaeI y'GGCC_r
Cuts at: 253 1112 1251 1335 2012 2186 2577 2604 3949
Size: 859 139 84 677 174 391 27 1345
Cuts at: 3949 253
Size: 731
Fragments arranged by size:
1345 859 731 677 391 174 139 84
27
EagI C'GGCC_G
Cuts at: 2012 2012
Size: 4427
EarI CTCTTCn'nnn_
Cuts at: 242 299 1237 1321 2450 2660 242
Size: 57 938 84 1129 210 2009
Fragments arranged by size:
2009 1129 938 210 84 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 492 2635 2945 3091 4237 492
Size: 2143 310 146 1146 682
Fragments arranged by size:
2143 1146 682 310 146
EcoICRI GAG'CTC
Cuts at: 1439 1439
Size: 4427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 301 307 401 764 789 820 2252 2684 3431
Size: 6 94 363 25 31 1432 432 747
Cuts at: 3431 3699 301
Size: 268 1029
Fragments arranged by size:
1432 1029 747 432 363 268 94 31
25 6
EcoNI CCTnn'n_nnAGG
Cuts at: 304 304
Size: 4427
EcoO109I rG'GnC_Cy
Cuts at: 274 426 615 823 824 1566 274
Size: 152 189 208 1 742 3135
Fragments arranged by size:
3135 742 208 189 152 1
EcoRI G'AATT_C
Cuts at: 1431 1431
Size: 4427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2446 2446
Size: 4427
FspI TGC'GCA
Cuts at: 2208 2208
Size: 4427
HaeII r_GCGC'y
Cuts at: 175 1861 2109 3131 4110 175
Size: 1686 248 1022 979 492
Fragments arranged by size:
1686 1022 979 492 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 109 141 301 333 503 535 835 867 1010
Size: 32 160 32 170 32 300 32 143
Cuts at: 1010 1042 2269 2299 2301 2331 109
Size: 32 1227 30 2 30 2205
Fragments arranged by size:
2205 1227 300 170 160 143 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1466 1466
Size: 4427
KasI G'GCGC_C
Cuts at: 1857 2105 1857
Size: 248 4179
KpnI G_GTAC'C
Cuts at: 1447 1447
Size: 4427
MluI A'CGCG_T
Cuts at: 3860 3860
Size: 4427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1146 3098 3282 1146
Size: 1952 184 2291
Fragments arranged by size:
2291 1952 184
MscI TGG'CCA
Cuts at: 1114 2188 1114
Size: 1074 3353
MslI CAynn'nnrTG
Cuts at: 874 1052 2416 2543 874
Size: 178 1364 127 2758
Fragments arranged by size:
2758 1364 178 127
MspA1I CmG'CkG
Cuts at: 207 400 986 1397 1538 1852 2212 3225 3470
Size: 193 586 411 141 314 360 1013 245
Cuts at: 3470 207
Size: 1164
Fragments arranged by size:
1164 1013 586 411 360 314 245 193
141
NaeI GCC'GGC
Cuts at: 2608 2608
Size: 4427
NarI GG'CG_CC
Cuts at: 1858 2106 1858
Size: 248 4179
NcoI C'CATG_G
Cuts at: 256 256
Size: 4427
NgoMIV G'CCGG_C
Cuts at: 2606 2606
Size: 4427
NspI r_CATG'y
Cuts at: 582 905 1135 1480 1980 2887 582
Size: 323 230 345 500 907 2122
Fragments arranged by size:
2122 907 500 345 323 230
PasI CC'CwG_GG
Cuts at: 347 347
Size: 4427
PciI A'CATG_T
Cuts at: 2883 2883
Size: 4427
PmeI GTTT'AAAC
Cuts at: 1426 1426
Size: 4427
PmlI CAC'GTG
Cuts at: 1224 1224
Size: 4427
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3566 3598 3566
Size: 32 4395
PpuMI rG'GwC_Cy
Cuts at: 274 615 274
Size: 341 4086
PspOMI G'GGCC_C
Cuts at: 823 823
Size: 4427
PstI C_TGCA'G
Cuts at: 144 1474 2159 144
Size: 1330 685 2412
Fragments arranged by size:
2412 1330 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4427
PvuII CAG'CTG
Cuts at: 207 400 986 1397 1852 2212 207
Size: 193 586 411 455 360 2422
Fragments arranged by size:
2422 586 455 411 360 193
RsrII CG'GwC_CG
Cuts at: 2622 2622
Size: 4427
SacI G_AGCT'C
Cuts at: 1441 1441
Size: 4427
SalI G'TCGA_C
Cuts at: 1464 1464
Size: 4427
SanDI GG'GwC_CC
Cuts at: 615 615
Size: 4427
SapI GCTCTTCn'nnn_
Cuts at: 242 1237 1321 2450 2660 242
Size: 995 84 1129 210 2009
Fragments arranged by size:
2009 1129 995 210 84
SbfI CC_TGCA'GG
Cuts at: 1474 1474
Size: 4427
ScaI AGT'ACT
Cuts at: 3944 3944
Size: 4427
SfcI C'TryA_G
Cuts at: 33 140 981 1470 2155 3148 3339 33
Size: 107 841 489 685 993 191 1121
Fragments arranged by size:
1121 993 841 685 489 191 107
SfoI GGC'GCC
Cuts at: 1859 2107 1859
Size: 248 4179
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4427
SmaI CCC'GGG
Cuts at: 1449 1449
Size: 4427
SmlI C'TyrA_G
Cuts at: 991 1582 2497 2989 3251 991
Size: 591 915 492 262 2167
Fragments arranged by size:
2167 915 591 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4427
SphI G_CATG'C
Cuts at: 1135 1480 1980 1135
Size: 345 500 3582
Fragments arranged by size:
3582 500 345
StyI C'CwwG_G
Cuts at: 256 1561 1915 256
Size: 1305 354 2768
Fragments arranged by size:
2768 1305 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1384 2010 2143 2602 2811 1384
Size: 626 133 459 209 3000
Fragments arranged by size:
3000 626 459 209 133
TatI w'GTAC_w
Cuts at: 201 3942 201
Size: 3741 686
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 274 306 3944 3976 274
Size: 32 3638 32 725
Fragments arranged by size:
3638 725 32 32
Tth111I GACn'n_nGTC
Cuts at: 1382 2224 1382
Size: 842 3585
XbaI T'CTAG_A
Cuts at: 1458 1458
Size: 4427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1922 1922
Size: 4427
XmaI C'CCGG_G
Cuts at: 1447 1447
Size: 4427
XmnI GAAnn'nnTTC
Cuts at: 1303 1303
Size: 4427
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PmeI PmlI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PshAI PsiI PspXI PsrI SacII SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI