Restriction map for
pF1KE0699
(Circular) (Six-base) MAPSORT of: pF1KE0699.seq Check: 9335 from: 1 to: 16802
pF1KE0699 16802 bp
With 182 enzymes: SgfI *
May 18, 2010 14:02 ..
AatII G_ACGT'C
Cuts at: 9977 9977
Size: 16802
AccI GT'mk_AC
Cuts at: 4193 5270 7157 9516 11774 13840 4193
Size: 1077 1887 2359 2258 2066 7155
Fragments arranged by size:
7155 2359 2258 2066 1887 1077
Acc65I G'GTAC_C
Cuts at: 13818 13818
Size: 16802
AclI AA'CG_TT
Cuts at: 16642 16642
Size: 16802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 403 466 1726 1980 2141 2893 3226 3805 3901
Size: 63 1260 254 161 752 333 579 96
Cuts at: 3901 4493 4926 5160 5584 5720 6424 7101 7517
Size: 592 433 234 424 136 704 677 416
Cuts at: 7517 7903 7912 8215 8599 10676 11359 11385 11504
Size: 386 9 303 384 2077 683 26 119
Cuts at: 11504 11952 11992 12988 13101 13192 14627 15059 15806
Size: 448 40 996 113 91 1435 432 747
Cuts at: 15806 16074 403
Size: 268 1131
Fragments arranged by size:
2077 1435 1260 1131 996 752 747 704
683 677 592 579 448 433 432 424
416 386 384 333 303 268 254 234
161 136 119 113 96 91 63 40
26 9
AflII C'TTAA_G
Cuts at: 5314 6913 13360 5314
Size: 1599 6447 8756
Fragments arranged by size:
8756 6447 1599
AflIII A'CryG_T
Cuts at: 1305 7370 12617 14154 15258 16235 1305
Size: 6065 5247 1537 1104 977 1872
Fragments arranged by size:
6065 5247 1872 1537 1104 977
AgeI A'CCGG_T
Cuts at: 15190 15190
Size: 16802
AleI CACnn'nnGTG
Cuts at: 12988 12988
Size: 16802
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 457 3743 9392 12455 457
Size: 3286 5649 3063 4804
Fragments arranged by size:
5649 4804 3286 3063
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2974 3006 9848 9880 2974
Size: 32 6842 32 9896
Fragments arranged by size:
9896 6842 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1101 1299 1383 1494 1698 2490 5671 7476 8145
Size: 198 84 111 204 792 3181 1805 669
Cuts at: 8145 12298 12558 12570 14107 15674 1101
Size: 4153 260 12 1537 1567 2229
Fragments arranged by size:
4153 3181 2229 1805 1567 1537 792 669
260 204 198 111 84 12
ApaLI G'TGCA_C
Cuts at: 15572 15572
Size: 16802
ApoI r'AATT_y
Cuts at: 1844 2002 3302 3631 4393 4414 4965 5194 5356
Size: 158 1300 329 762 21 551 229 162
Cuts at: 5356 5896 6259 6486 6768 7360 7466 7639 8053
Size: 540 363 227 282 592 106 173 414
Cuts at: 8053 8266 8326 9068 9210 9270 9408 9586 9848
Size: 213 60 742 142 60 138 178 262
Cuts at: 9848 10318 10403 10422 10483 10671 10752 11293 11328
Size: 470 85 19 61 188 81 541 35
Cuts at: 11328 11476 12649 13069 13189 13642 13806 16155 16411
Size: 148 1173 420 120 453 164 2349 256
Cuts at: 16411 1844
Size: 2235
Fragments arranged by size:
2349 2235 1300 1173 762 742 592 551
541 540 470 453 420 414 363 329
282 262 256 229 227 213 188 178
173 164 162 158 148 142 138 120
106 85 81 61 60 60 35 21
19
AseI AT'TA_AT
Cuts at: 20 3796 5015 8564 9997 13085 20
Size: 3776 1219 3549 1433 3088 3737
Fragments arranged by size:
3776 3737 3549 3088 1433 1219
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 16802
AvaI C'yCGr_G
Cuts at: 1363 4103 10625 11467 13822 1363
Size: 2740 6522 842 2355 4343
Fragments arranged by size:
6522 4343 2740 2355 842
AvrII C'CTAG_G
Cuts at: 9963 9963
Size: 16802
BamHI G'GATC_C
Cuts at: 2531 4629 11095 13827 2531
Size: 2098 6466 2732 5506
Fragments arranged by size:
6466 5506 2732 2098
BanI G'GyrC_C
Cuts at: 178 2512 3649 4693 6043 10255 12127 12514 13818
Size: 2334 1137 1044 1350 4212 1872 387 1304
Cuts at: 13818 14232 14480 14515 178
Size: 414 248 35 2465
Fragments arranged by size:
4212 2465 2334 1872 1350 1304 1137 1044
414 387 248 35
BanII G_rGCy'C
Cuts at: 500 1370 2569 3173 3814 4269 5341 6206 9833
Size: 870 1199 604 641 455 1072 865 3627
Cuts at: 9833 12392 13816 14846 500
Size: 2559 1424 1030 2456
Fragments arranged by size:
3627 2559 2456 1424 1199 1072 1030 870
865 641 604 455
BbeI G_GCGC'C
Cuts at: 182 14236 14484 182
Size: 14054 248 2500
Fragments arranged by size:
14054 2500 248
BbsI GAAGACnn'nnnn_
Cuts at: 391 980 1093 1622 3832 5784 7909 10682 11525
Size: 589 113 529 2210 1952 2125 2773 843
Cuts at: 11525 12401 391
Size: 876 4792
Fragments arranged by size:
4792 2773 2210 2125 1952 876 843 589
529 113
BbvCI CC'TCA_GC
Cuts at: 1371 2061 8454 12105 1371
Size: 690 6393 3651 6068
Fragments arranged by size:
6393 6068 3651 690
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 727 761 727
Size: 34 16768
BciVI GTATCCnnnnn_n'
Cuts at: 639 3192 6858 9850 14700 15461 16802 639
Size: 2553 3666 2992 4850 761 1341 639
Fragments arranged by size:
4850 3666 2992 2553 1341 761 639
BclI T'GATC_A
Cuts at: 821 6420 6651 13337 14322 821
Size: 5599 231 6686 985 3301
Fragments arranged by size:
6686 5599 3301 985 231
BfrBI ATG'CAT
Cuts at: 4477 9751 4477
Size: 5274 11528
BglI GCCn_nnn'nGGC
Cuts at: 1693 1693
Size: 16802
BglII A'GATC_T
Cuts at: 1391 1935 4032 11370 14317 16354 1391
Size: 544 2097 7338 2947 2037 1839
Fragments arranged by size:
7338 2947 2097 2037 1839 544
BlpI GC'TnA_GC
Cuts at: 1441 6032 7237 10549 13914 1441
Size: 4591 1205 3312 3365 4329
Fragments arranged by size:
4591 4329 3365 3312 1205
Bme1580I G_kGCm'C
Cuts at: 2517 6018 9354 10258 10711 12332 14427 14520 15576
Size: 3501 3336 904 453 1621 2095 93 1056
Cuts at: 15576 2517
Size: 3743
Fragments arranged by size:
3743 3501 3336 2095 1621 1056 904 453
93
BmgBI CAC'GTC
Cuts at: 12489 12489
Size: 16802
BmrI ACTGGGnnnn_n'
Cuts at: 1553 2277 4441 5245 5888 9170 9341 10922 11571
Size: 724 2164 804 643 3282 171 1581 649
Cuts at: 11571 12384 12530 14014 14095 14430 16574 1553
Size: 813 146 1484 81 335 2144 1781
Fragments arranged by size:
3282 2164 2144 1781 1581 1484 813 804
724 649 643 335 171 146 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1162 2452 11718 11793 15169 1162
Size: 1290 9266 75 3376 2795
Fragments arranged by size:
9266 3376 2795 1290 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1078 1276 1601 2467 2648 2681 2708 2860
Size: 705 198 325 866 181 33 27 152
Cuts at: 2860 3157 3880 4011 4349 5128 6011 8583 8728
Size: 297 723 131 338 779 883 2572 145
Cuts at: 8728 10781 12185 13757 373
Size: 2053 1404 1572 3418
Fragments arranged by size:
3418 2572 2053 1572 1404 883 866 779
723 705 338 325 297 198 181 152
145 131 33 27
Bpu10I CC'TnA_GC
Cuts at: 1371 2061 5678 8454 11681 12105 13526 1371
Size: 690 3617 2776 3227 424 1421 4647
Fragments arranged by size:
4647 3617 3227 2776 1421 690 424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 1609 3203 4999 6040 7069 10030 12592 12629
Size: 1125 1594 1796 1041 1029 2961 2562 37
Cuts at: 12629 12961 13205 13401 13978 14857 15349 15647 484
Size: 332 244 196 577 879 492 298 1639
Fragments arranged by size:
2961 2562 1796 1639 1594 1125 1041 1029
879 577 492 332 298 244 196 37
BsaI GGTCTCn'nnnn_
Cuts at: 722 2892 2946 4385 6325 7661 16506 722
Size: 2170 54 1439 1940 1336 8845 1018
Fragments arranged by size:
8845 2170 1940 1439 1336 1018 54
BsaAI yAC'GTr
Cuts at: 3783 12997 14155 3783
Size: 9214 1158 6430
Fragments arranged by size:
9214 6430 1158
BsaBI GATnn'nnATC
Cuts at: 6 8180 12065 13708 14316 16353 6
Size: 8174 3885 1643 608 2037 455
Fragments arranged by size:
8174 3885 2037 1643 608 455
BsaHI Gr'CG_yC
Cuts at: 179 9974 14233 14481 15183 16679 179
Size: 9795 4259 248 702 1496 302
Fragments arranged by size:
9795 4259 1496 702 302 248
BsaWI w'CCGG_w
Cuts at: 254 2781 3306 11018 13991 14512 15190 15464 15611
Size: 2527 525 7712 2973 521 678 274 147
Cuts at: 15611 16022 254
Size: 411 1034
Fragments arranged by size:
7712 2973 2527 1034 678 525 521 411
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1044 1074 3046 3061 3076 3091 3533 3563 4052
Size: 30 1972 15 15 15 442 30 489
Cuts at: 4052 4082 5935 5965 8714 8744 9525 9555 11447
Size: 30 1853 30 2749 30 781 30 1892
Cuts at: 11447 11477 11793 11823 1044
Size: 30 316 30 6023
Fragments arranged by size:
6023 2749 1972 1892 1853 781 489 442
316 30 30 30 30 30 30 30
30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 481 1821 2467 4668 8396 9530 481
Size: 1340 646 2201 3728 1134 7753
Fragments arranged by size:
7753 3728 2201 1340 1134 646
BseYI C'CCAG_C
Cuts at: 362 2055 2863 5725 9089 9230 9353 12048 12117
Size: 1693 808 2862 3364 141 123 2695 69
Cuts at: 12117 14227 14985 15562 16058 362
Size: 2110 758 577 496 1106
Fragments arranged by size:
3364 2862 2695 2110 1693 1106 808 758
577 496 141 123 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1524 3018 6011 11744 11993 13423 1524
Size: 1494 2993 5733 249 1430 4903
Fragments arranged by size:
5733 4903 2993 1494 1430 249
BsiEI CG_ry'CG
Cuts at: 104 14344 14390 15598 104
Size: 14240 46 1208 1308
Fragments arranged by size:
14240 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 500 1045 1279 3173 3814 5028 5755 6752 9833
Size: 545 234 1894 641 1214 727 997 3081
Cuts at: 9833 10684 10802 13816 14594 14784 15576 500
Size: 851 118 3014 778 190 792 1726
Fragments arranged by size:
3081 3014 1894 1726 1214 997 851 792
778 727 641 545 234 190 118
BsmI GAATG_Cn'
Cuts at: 2613 5127 9657 9913 10980 2613
Size: 2514 4530 256 1067 8435
Fragments arranged by size:
8435 4530 2514 1067 256
Bsp1286I G_dGCh'C
Cuts at: 500 1045 1279 1370 2517 2569 3173 3814 4269
Size: 545 234 91 1147 52 604 641 455
Cuts at: 4269 5028 5341 5755 6018 6206 6752 9354 9833
Size: 759 313 414 263 188 546 2602 479
Cuts at: 9833 10258 10684 10711 10802 12332 12392 13816 14427
Size: 425 426 27 91 1530 60 1424 611
Cuts at: 14427 14520 14594 14784 14846 15576 500
Size: 93 74 190 62 730 1726
Fragments arranged by size:
2602 1726 1530 1424 1147 759 730 641
611 604 546 545 479 455 426 425
414 313 263 234 190 188 93 91
91 74 62 60 52 27
BspEI T'CCGG_A
Cuts at: 254 2781 3306 11018 16022 254
Size: 2527 525 7712 5004 1034
Fragments arranged by size:
7712 5004 2527 1034 525
BspHI T'CATG_A
Cuts at: 5937 13748 15978 5937
Size: 7811 2230 6761
Fragments arranged by size:
7811 6761 2230
BspMI ACCTGCnnnn'nnnn_
Cuts at: 516 4310 8458 8831 11730 13852 14066 14368 14749
Size: 3794 4148 373 2899 2122 214 302 381
Cuts at: 14749 516
Size: 2569
Fragments arranged by size:
4148 3794 2899 2569 2122 381 373 302
214
BsrBI CCG'CTC
Cuts at: 50 15094 15148 16632 16797 50
Size: 15044 54 1484 165 55
Fragments arranged by size:
15044 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 705 1502 2666 3812 5381 5793 5853 7248 11087
Size: 797 1164 1146 1569 412 60 1395 3839
Cuts at: 11087 11609 13550 14714 705
Size: 522 1941 1164 2793
Fragments arranged by size:
3839 2793 1941 1569 1395 1164 1164 1146
797 522 412 60
BsrFI r'CCGG_y
Cuts at: 4643 14800 14981 15190 4643
Size: 10157 181 209 6255
Fragments arranged by size:
10157 6255 209 181
BsrGI T'GTAC_A
Cuts at: 4205 11234 4205
Size: 7029 9773
BssSI C'ACGA_G
Cuts at: 2827 5968 7664 15073 15431 2827
Size: 3141 1696 7409 358 4198
Fragments arranged by size:
7409 4198 3141 1696 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3229 11758 13437 14179 3229
Size: 8529 1679 742 5852
Fragments arranged by size:
8529 5852 1679 742
BstBI TT'CG_AA
Cuts at: 4405 12805 15163 4405
Size: 8400 2358 6044
Fragments arranged by size:
8400 6044 2358
BstEII G'GTnAC_C
Cuts at: 1338 5581 1338
Size: 4243 12559
BstXI CCAn_nnnn'nTGG
Cuts at: 2545 3600 5262 2545
Size: 1055 1662 14085
Fragments arranged by size:
14085 1662 1055
BstYI r'GATC_y
Cuts at: 1 1391 1935 2531 3515 3693 4032 4324 4629
Size: 1390 544 596 984 178 339 292 305
Cuts at: 4629 5526 8190 11095 11370 11622 13235 13827 14294
Size: 897 2664 2905 275 252 1613 592 467
Cuts at: 14294 14317 14652 14898 15910 15996 16008 16354 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
2905 2664 1613 1390 1012 984 897 596
592 544 467 449 346 339 335 305
292 275 252 246 178 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 428 3039 4666 11113 428
Size: 2611 1627 6447 6117
Fragments arranged by size:
6447 6117 2611 1627
BtgI C'CryG_G
Cuts at: 107 1601 1852 4971 10106 10478 10885 12131 107
Size: 1494 251 3119 5135 372 407 1246 4778
Fragments arranged by size:
5135 4778 3119 1494 1246 407 372 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3965 5706 14752 14933 15096 3965
Size: 1741 9046 181 163 5671
Fragments arranged by size:
9046 5671 1741 181 163
BtsI GCAGTG_nn'
Cuts at: 745 2138 4894 5773 7547 8167 8486 10234 13520
Size: 1393 2756 879 1774 620 319 1748 3286
Cuts at: 13520 14045 745
Size: 525 3502
Fragments arranged by size:
3502 3286 2756 1774 1748 1393 879 620
525 319
ClaI AT'CG_AT
Cuts at: 5828 5828
Size: 16802
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3421 3456 8657 8692 11175 11210 3421
Size: 35 5201 35 2483 35 9013
Fragments arranged by size:
9013 5201 2483 35 35 35
DraI TTT'AAA
Cuts at: 4817 5498 7335 9182 10137 12960 13143 13801 4817
Size: 681 1837 1847 955 2823 183 658 7818
Fragments arranged by size:
7818 2823 1847 1837 955 681 658 183
DraIII CAC_nnn'GTG
Cuts at: 11962 11962
Size: 16802
DrdI GACnn_nn'nnGTC
Cuts at: 12789 14508 15366 12789
Size: 1719 858 14225
Fragments arranged by size:
14225 1719 858
EaeI y'GGCC_r
Cuts at: 506 3954 4641 5049 5169 8856 10956 14387 14561
Size: 3448 687 408 120 3687 2100 3431 174
Cuts at: 14561 14952 14979 16324 506
Size: 391 27 1345 984
Fragments arranged by size:
3687 3448 3431 2100 1345 984 687 408
391 174 120 27
EagI C'GGCC_G
Cuts at: 14387 14387
Size: 16802
EarI CTCTTCn'nnn_
Cuts at: 185 910 1153 2045 3614 3943 4590 5412 5640
Size: 725 243 892 1569 329 647 822 228
Cuts at: 5640 7931 10061 10476 12281 14825 15035 185
Size: 2291 2130 415 1805 2544 210 1952
Fragments arranged by size:
2544 2291 2130 1952 1805 1569 892 822
725 647 415 329 243 228 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3063 4206 15010 15320 15466 16612 3063
Size: 1143 10804 310 146 1146 3253
Fragments arranged by size:
10804 3253 1146 1143 310 146
EcoICRI GAG'CTC
Cuts at: 498 3171 3812 9831 13814 498
Size: 2673 641 6019 3983 3486
Fragments arranged by size:
6019 3983 3486 2673 641
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 403 466 1078 1276 1601 1726 1980 2141
Size: 30 63 612 198 325 125 254 161
Cuts at: 2141 2467 2648 2681 2708 2860 2893 3157 3226
Size: 326 181 33 27 152 33 264 69
Cuts at: 3226 3805 3880 3901 4011 4349 4493 4926 5128
Size: 579 75 21 110 338 144 433 202
Cuts at: 5128 5160 5584 5720 6011 6424 7101 7517 7903
Size: 32 424 136 291 413 677 416 386
Cuts at: 7903 7912 8215 8583 8599 8728 10676 10781 11359
Size: 9 303 368 16 129 1948 105 578
Cuts at: 11359 11385 11504 11952 11992 12185 12988 13101 13192
Size: 26 119 448 40 193 803 113 91
Cuts at: 13192 13757 14627 15059 15806 16074 373
Size: 565 870 432 747 268 1101
Fragments arranged by size:
1948 1101 870 803 747 677 612 579
578 565 448 433 432 424 416 413
386 368 338 326 325 303 291 268
264 254 202 198 193 181 161 152
144 136 129 125 119 113 110 105
91 75 69 63 40 33 33 32
30 27 26 21 16 9
EcoNI CCTnn'n_nnAGG
Cuts at: 406 9720 10957 406
Size: 9314 1237 6251
Fragments arranged by size:
9314 6251 1237
EcoO109I rG'GnC_Cy
Cuts at: 1484 8962 9839 10830 12345 12524 13008 13941 1484
Size: 7478 877 991 1515 179 484 933 4345
Fragments arranged by size:
7478 4345 1515 991 933 877 484 179
EcoRI G'AATT_C
Cuts at: 6486 8266 8326 9586 10671 12649 13806 6486
Size: 1780 60 1260 1085 1978 1157 9482
Fragments arranged by size:
9482 1978 1780 1260 1157 1085 60
EcoRV GAT'ATC
Cuts at: 1929 10728 12483 1929
Size: 8799 1755 6248
Fragments arranged by size:
8799 6248 1755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2098 2595 3030 5329 6205 7933 9330 9606 10569
Size: 497 435 2299 876 1728 1397 276 963
Cuts at: 10569 14821 2098
Size: 4252 4079
Fragments arranged by size:
4252 4079 2299 1728 1397 963 876 497
435 276
FspI TGC'GCA
Cuts at: 8827 14583 8827
Size: 5756 11046
HaeII r_GCGC'y
Cuts at: 139 148 182 3047 14236 14484 15506 16485 139
Size: 9 34 2865 11189 248 1022 979 456
Fragments arranged by size:
11189 2865 1022 979 456 248 34 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1128 1160 1794 1826 2044 2076 2281 2313 2389
Size: 32 634 32 218 32 205 32 76
Cuts at: 2389 2421 2540 2572 2955 2987 3061 3093 4912
Size: 32 119 32 383 32 74 32 1819
Cuts at: 4912 4944 5047 5079 5303 5335 6292 6324 7112
Size: 32 103 32 224 32 957 32 788
Cuts at: 7112 7144 8650 8682 8714 8727 8746 8759 11193
Size: 32 1506 32 32 13 19 13 2434
Cuts at: 11193 11225 12411 12443 12889 12921 13317 13349 13609
Size: 32 1186 32 446 32 396 32 260
Cuts at: 13609 13641 14644 14674 14676 14706 1128
Size: 32 1003 30 2 30 3224
Fragments arranged by size:
3224 2434 1819 1506 1186 1003 957 788
634 446 396 383 260 224 218 205
119 103 76 74 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
30 30 19 13 13 2
HincII GTy'rAC
Cuts at: 815 1455 1613 2253 3321 4299 5271 7356 9299
Size: 640 158 640 1068 978 972 2085 1943
Cuts at: 9299 10539 11498 11567 12363 12575 13841 815
Size: 1240 959 69 796 212 1266 3776
Fragments arranged by size:
3776 2085 1943 1266 1240 1068 978 972
959 796 640 640 212 158 69
HindIII A'AGCT_T
Cuts at: 2258 7315 10048 10546 13214 13765 2258
Size: 5057 2733 498 2668 551 5295
Fragments arranged by size:
5295 5057 2733 2668 551 498
HpaI GTT'AAC
Cuts at: 1613 7356 9299 11498 11567 1613
Size: 5743 1943 2199 69 6848
Fragments arranged by size:
6848 5743 2199 1943 69
KasI G'GCGC_C
Cuts at: 178 14232 14480 178
Size: 14054 248 2500
Fragments arranged by size:
14054 2500 248
KpnI G_GTAC'C
Cuts at: 13822 13822
Size: 16802
MfeI C'AATT_G
Cuts at: 6266 6495 7006 7587 8093 8354 6266
Size: 229 511 581 506 261 14714
Fragments arranged by size:
14714 581 511 506 261 229
MluI A'CGCG_T
Cuts at: 16235 16235
Size: 16802
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2005 3077 4685 7269 10800 13053 15473 15657 2005
Size: 1072 1608 2584 3531 2253 2420 184 3150
Fragments arranged by size:
3531 3150 2584 2420 2253 1608 1072 184
MscI TGG'CCA
Cuts at: 508 5051 8858 10958 14563 508
Size: 4543 3807 2100 3605 2747
Fragments arranged by size:
4543 3807 3605 2747 2100
MslI CAynn'nnrTG
Cuts at: 2599 3614 3725 4368 5624 5746 5840 6761 8627
Size: 1015 111 643 1256 122 94 921 1866
Cuts at: 8627 8895 9182 9904 11213 11674 12142 12988 13747
Size: 268 287 722 1309 461 468 846 759
Cuts at: 13747 14791 14918 2599
Size: 1044 127 4483
Fragments arranged by size:
4483 1866 1309 1256 1044 1015 921 846
759 722 643 468 461 287 268 127
122 111 94
MspA1I CmG'CkG
Cuts at: 1215 1383 2161 3090 5886 6031 8571 13056 13913
Size: 168 778 929 2796 145 2540 4485 857
Cuts at: 13913 14227 14587 15600 15845 1215
Size: 314 360 1013 245 2172
Fragments arranged by size:
4485 2796 2540 2172 1013 929 857 778
360 314 245 168 145
NaeI GCC'GGC
Cuts at: 4645 14983 4645
Size: 10338 6464
NarI GG'CG_CC
Cuts at: 179 14233 14481 179
Size: 14054 248 2500
Fragments arranged by size:
14054 2500 248
NcoI C'CATG_G
Cuts at: 107 1601 1852 4971 10106 10478 10885 107
Size: 1494 251 3119 5135 372 407 6024
Fragments arranged by size:
6024 5135 3119 1494 407 372 251
NdeI CA'TA_TG
Cuts at: 3722 4341 4608 5343 12656 13134 3722
Size: 619 267 735 7313 478 7390
Fragments arranged by size:
7390 7313 735 619 478 267
NgoMIV G'CCGG_C
Cuts at: 4643 14981 4643
Size: 10338 6464
NsiI A_TGCA'T
Cuts at: 4479 9753 4479
Size: 5274 11528
NspI r_CATG'y
Cuts at: 874 1315 2124 2715 5575 6520 7057 7374 9097
Size: 441 809 591 2860 945 537 317 1723
Cuts at: 9097 9948 11858 13855 14355 15262 874
Size: 851 1910 1997 500 907 2414
Fragments arranged by size:
2860 2414 1997 1910 1723 945 907 851
809 591 537 500 441 317
PasI CC'CwG_GG
Cuts at: 301 6450 301
Size: 6149 10653
PciI A'CATG_T
Cuts at: 7370 15258 7370
Size: 7888 8914
PflMI CCAn_nnn'nTGG
Cuts at: 1602 2490 3903 6093 9027 9096 10965 1602
Size: 888 1413 2190 2934 69 1869 7439
Fragments arranged by size:
7439 2934 2190 1869 1413 888 69
PfoI T'CCnGG_A
Cuts at: 1094 1767 2417 2673 3740 6847 9852 11843 12242
Size: 673 650 256 1067 3107 3005 1991 399
Cuts at: 12242 13773 1094
Size: 1531 4123
Fragments arranged by size:
4123 3107 3005 1991 1531 1067 673 650
399 256
PmeI GTTT'AAAC
Cuts at: 13801 13801
Size: 16802
PmlI CAC'GTG
Cuts at: 3783 3783
Size: 16802
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7843 7875 15941 15973 7843
Size: 32 8066 32 8672
Fragments arranged by size:
8672 8066 32 32
PpuMI rG'GwC_Cy
Cuts at: 1484 8962 9839 12345 12524 13008 1484
Size: 7478 877 2506 179 484 5278
Fragments arranged by size:
7478 5278 2506 877 484 179
PsiI TTA'TAA
Cuts at: 5447 7072 5447
Size: 1625 15177
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 12166 12198 12166
Size: 32 16770
PstI C_TGCA'G
Cuts at: 373 480 2528 6632 6908 8162 10241 10706 12434
Size: 107 2048 4104 276 1254 2079 465 1728
Cuts at: 12434 13849 14534 373
Size: 1415 685 2641
Fragments arranged by size:
4104 2641 2079 2048 1728 1415 1254 685
465 276 107
PvuI CG_AT'CG
Cuts at: 104 104
Size: 16802
PvuII CAG'CTG
Cuts at: 1215 1383 2161 5886 6031 13056 14227 14587 1215
Size: 168 778 3725 145 7025 1171 360 3430
Fragments arranged by size:
7025 3725 3430 1171 778 360 168 145
RsrII CG'GwC_CG
Cuts at: 14997 14997
Size: 16802
SacI G_AGCT'C
Cuts at: 500 3173 3814 9833 13816 500
Size: 2673 641 6019 3983 3486
Fragments arranged by size:
6019 3983 3486 2673 641
SalI G'TCGA_C
Cuts at: 5269 13839 5269
Size: 8570 8232
SanDI GG'GwC_CC
Cuts at: 1484 1484
Size: 16802
SapI GCTCTTCn'nnn_
Cuts at: 7931 14825 15035 7931
Size: 6894 210 9698
Fragments arranged by size:
9698 6894 210
SbfI CC_TGCA'GG
Cuts at: 13849 13849
Size: 16802
ScaI AGT'ACT
Cuts at: 6840 10344 16319 6840
Size: 3504 5975 7323
Fragments arranged by size:
7323 5975 3504
SexAI A'CCwGG_T
Cuts at: 1346 1346
Size: 16802
SfcI C'TryA_G
Cuts at: 33 369 476 1478 2524 3128 3569 4194 4224
Size: 336 107 1002 1046 604 441 625 30
Cuts at: 4224 5470 6628 6904 7558 8158 10237 10702 12183
Size: 1246 1158 276 654 600 2079 465 1481
Cuts at: 12183 12430 13845 14530 15523 15714 33
Size: 247 1415 685 993 191 1121
Fragments arranged by size:
2079 1481 1415 1246 1158 1121 1046 1002
993 685 654 625 604 600 465 441
336 276 247 191 107 30
SfoI GGC'GCC
Cuts at: 180 14234 14482 180
Size: 14054 248 2500
Fragments arranged by size:
14054 2500 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 16802
SmaI CCC'GGG
Cuts at: 13824 13824
Size: 16802
SmlI C'TyrA_G
Cuts at: 499 1624 3218 4103 4978 5314 6055 6913 7048
Size: 1125 1594 885 875 336 741 858 135
Cuts at: 7048 10045 12607 12644 12976 13220 13360 13416 13957
Size: 2997 2562 37 332 244 140 56 541
Cuts at: 13957 14872 15364 15626 499
Size: 915 492 262 1675
Fragments arranged by size:
2997 2562 1675 1594 1125 915 885 875
858 741 541 492 336 332 262 244
140 135 56 37
SnaBI TAC'GTA
Cuts at: 12997 12997
Size: 16802
SpeI A'CTAG_T
Cuts at: 67 67
Size: 16802
SphI G_CATG'C
Cuts at: 1315 2124 9948 13855 14355 1315
Size: 809 7824 3907 500 3762
Fragments arranged by size:
7824 3907 3762 809 500
SspI AAT'ATT
Cuts at: 4469 6346 6417 6708 6813 7810 10116 10633 11506
Size: 1877 71 291 105 997 2306 517 873
Cuts at: 11506 13270 13306 13449 4469
Size: 1764 36 143 7822
Fragments arranged by size:
7822 2306 1877 1764 997 873 517 291
143 105 71 36
StuI AGG'CCT
Cuts at: 1178 3126 1178
Size: 1948 14854
StyI C'CwwG_G
Cuts at: 107 1250 1601 1852 2681 3191 4689 4971 6521
Size: 1143 351 251 829 510 1498 282 1550
Cuts at: 6521 7526 7887 9963 10106 10478 10885 10953 12332
Size: 1005 361 2076 143 372 407 68 1379
Cuts at: 12332 13936 14290 107
Size: 1604 354 2619
Fragments arranged by size:
2619 2076 1604 1550 1498 1379 1143 1005
829 510 407 372 361 354 351 282
251 143 68
SwaI ATTT'AAAT
Cuts at: 9182 9182
Size: 16802
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2500 4156 5254 7685 14385 14518 14977 15186 2500
Size: 1656 1098 2431 6700 133 459 209 4116
Fragments arranged by size:
6700 4116 2431 1656 1098 459 209 133
TatI w'GTAC_w
Cuts at: 260 845 1654 2264 4205 6378 6838 9398 9803
Size: 585 809 610 1941 2173 460 2560 405
Cuts at: 9803 10241 10342 10514 11234 16317 260
Size: 438 101 172 720 5083 745
Fragments arranged by size:
5083 2560 2173 1941 809 745 720 610
585 460 438 405 172 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1044 1076 1658 1690 3772 3804 4052 4084 8068
Size: 32 582 32 2082 32 248 32 3984
Cuts at: 8068 8100 9525 9557 9643 9675 16319 16351 1044
Size: 32 1425 32 86 32 6644 32 1495
Fragments arranged by size:
6644 3984 2082 1495 1425 582 248 86
32 32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2248 3285 14599 2248
Size: 1037 11314 4451
Fragments arranged by size:
11314 4451 1037
XbaI T'CTAG_A
Cuts at: 4173 4398 7653 13833 4173
Size: 225 3255 6180 7142
Fragments arranged by size:
7142 6180 3255 225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3111 3687 6949 10365 14297 3111
Size: 576 3262 3416 3932 5616
Fragments arranged by size:
5616 3932 3416 3262 576
XhoI C'TCGA_G
Cuts at: 4103 4103
Size: 16802
XmaI C'CCGG_G
Cuts at: 13822 13822
Size: 16802
XmnI GAAnn'nnTTC
Cuts at: 1526 3886 4357 6490 9553 9586 12005 13193 1526
Size: 2360 471 2133 3063 33 2419 1188 5135
Fragments arranged by size:
5135 3063 2419 2360 2133 1188 471 33
ZraI GAC'GTC
Cuts at: 9975 9975
Size: 16802
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI ApaI AscI BaeI BmtI BsiWI
BsmBI BssHII BstZ17I FseI FspAI NheI NotI NruI
PacI PshAI PspOMI PspXI SacII SfiI SgrAI SrfI