Restriction map for
pF1KE0693
(Circular) (Six-base) MAPSORT of: pF1KE0693.seq Check: 5236 from: 1 to: 3959
pF1KE0693 3959 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 807 807
Size: 3959
AccI GT'mk_AC
Cuts at: 997 997
Size: 3959
Acc65I G'GTAC_C
Cuts at: 975 975
Size: 3959
AclI AA'CG_TT
Cuts at: 3799 3799
Size: 3959
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1784 2216 2963 3231 1784
Size: 432 747 268 2512
Fragments arranged by size:
2512 747 432 268
AflIII A'CryG_T
Cuts at: 1311 2415 3392 1311
Size: 1104 977 1878
Fragments arranged by size:
1878 1104 977
AgeI A'CCGG_T
Cuts at: 2347 2347
Size: 3959
AlwNI CAG_nnn'CTG
Cuts at: 1264 2831 1264
Size: 1567 2392
ApaLI G'TGCA_C
Cuts at: 2729 2729
Size: 3959
ApoI r'AATT_y
Cuts at: 674 963 3312 3568 674
Size: 289 2349 256 1065
Fragments arranged by size:
2349 1065 289 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3959
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
AvaI C'yCGr_G
Cuts at: 584 979 584
Size: 395 3564
BamHI G'GATC_C
Cuts at: 984 984
Size: 3959
BanI G'GyrC_C
Cuts at: 975 1389 1637 1672 975
Size: 414 248 35 3262
Fragments arranged by size:
3262 414 248 35
BanII G_rGCy'C
Cuts at: 973 2003 973
Size: 1030 2929
BbeI G_GCGC'C
Cuts at: 1393 1641 1393
Size: 248 3711
BbsI GAAGACnn'nnnn_
Cuts at: 432 863 885 432
Size: 431 22 3506
Fragments arranged by size:
3506 431 22
BciVI GTATCCnnnnn_n'
Cuts at: 1857 2618 3959 1857
Size: 761 1341 1857
Fragments arranged by size:
1857 1341 761
BclI T'GATC_A
Cuts at: 564 1479 564
Size: 915 3044
BglII A'GATC_T
Cuts at: 925 1474 3511 925
Size: 549 2037 1373
Fragments arranged by size:
2037 1373 549
BlpI GC'TnA_GC
Cuts at: 1071 1071
Size: 3959
Bme1580I G_kGCm'C
Cuts at: 1584 1677 2733 1584
Size: 93 1056 2810
Fragments arranged by size:
2810 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 584 1171 1252 1587 3731 584
Size: 587 81 335 2144 812
Fragments arranged by size:
2144 812 587 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 168 314 368 2326 168
Size: 146 54 1958 1801
Fragments arranged by size:
1958 1801 146 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1135 2014 2506 2804 1135
Size: 879 492 298 2290
Fragments arranged by size:
2290 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3663 3663
Size: 3959
BsaAI yAC'GTr
Cuts at: 1312 1312
Size: 3959
BsaBI GATnn'nnATC
Cuts at: 6 264 270 321 912 918 924 1473 3510
Size: 258 6 51 591 6 6 549 2037
Cuts at: 3510 6
Size: 455
Fragments arranged by size:
2037 591 549 455 258 51 6 6
6
BsaHI Gr'CG_yC
Cuts at: 804 1390 1638 2340 3836 804
Size: 586 248 702 1496 927
Fragments arranged by size:
1496 927 702 586 248
BsaWI w'CCGG_w
Cuts at: 419 1148 1669 2347 2621 2768 3179 419
Size: 729 521 678 274 147 411 1199
Fragments arranged by size:
1199 729 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 162 190 192 220 162
Size: 28 2 28 3901
Fragments arranged by size:
3901 28 28 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 782 782
Size: 3959
BseYI C'CCAG_C
Cuts at: 1384 2142 2719 3215 1384
Size: 758 577 496 2128
Fragments arranged by size:
2128 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1501 1547 2755 104
Size: 1397 46 1208 1308
Fragments arranged by size:
1397 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 973 1751 1941 2733 973
Size: 778 190 792 2199
Fragments arranged by size:
2199 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 339 339
Size: 3959
Bsp1286I G_dGCh'C
Cuts at: 973 1584 1677 1751 1941 2003 2733 973
Size: 611 93 74 190 62 730 2199
Fragments arranged by size:
2199 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3179 3179
Size: 3959
BspHI T'CATG_A
Cuts at: 3135 3135
Size: 3959
BspMI ACCTGCnnnn'nnnn_
Cuts at: 194 1009 1223 1525 1906 194
Size: 815 214 302 381 2247
Fragments arranged by size:
2247 815 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2251 2305 3789 3954 50
Size: 2201 54 1484 165 55
Fragments arranged by size:
2201 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1871 1871
Size: 3959
BsrFI r'CCGG_y
Cuts at: 380 1957 2138 2347 380
Size: 1577 181 209 1992
Fragments arranged by size:
1992 1577 209 181
BsrGI T'GTAC_A
Cuts at: 491 491
Size: 3959
BssSI C'ACGA_G
Cuts at: 185 2230 2588 185
Size: 2045 358 1556
Fragments arranged by size:
2045 1556 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1336 1336
Size: 3959
BstBI TT'CG_AA
Cuts at: 2320 2320
Size: 3959
BstYI r'GATC_y
Cuts at: 1 211 322 453 507 925 984 1451 1474
Size: 210 111 131 54 418 59 467 23
Cuts at: 1474 1809 2055 3067 3153 3165 3511 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 418 346 335 246 210
131 111 86 59 54 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1909 2090 2253 1909
Size: 181 163 3615
Fragments arranged by size:
3615 181 163
BtsI GCAGTG_nn'
Cuts at: 1202 1202
Size: 3959
DraI TTT'AAA
Cuts at: 958 958
Size: 3959
DrdI GACnn_nn'nnGTC
Cuts at: 1665 2523 1665
Size: 858 3101
EaeI y'GGCC_r
Cuts at: 1544 1718 2109 2136 3481 1544
Size: 174 391 27 1345 2022
Fragments arranged by size:
2022 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1544 1544
Size: 3959
EarI CTCTTCn'nnn_
Cuts at: 1982 2192 1982
Size: 210 3749
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2167 2477 2623 3769 2167
Size: 310 146 1146 2357
Fragments arranged by size:
2357 1146 310 146
EcoICRI GAG'CTC
Cuts at: 971 971
Size: 3959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1784 2216 2963 3231 1784
Size: 432 747 268 2512
Fragments arranged by size:
2512 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1098 1098
Size: 3959
EcoRI G'AATT_C
Cuts at: 674 963 674
Size: 289 3670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1978 1978
Size: 3959
FspI TGC'GCA
Cuts at: 1740 1740
Size: 3959
HaeII r_GCGC'y
Cuts at: 1393 1641 2663 3642 1393
Size: 248 1022 979 1710
Fragments arranged by size:
1710 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 1801 1831 1833 1863 190
Size: 32 1579 30 2 30 2286
Fragments arranged by size:
2286 1579 32 30 30 2
HincII GTy'rAC
Cuts at: 998 998
Size: 3959
KasI G'GCGC_C
Cuts at: 1389 1637 1389
Size: 248 3711
KpnI G_GTAC'C
Cuts at: 979 979
Size: 3959
MluI A'CGCG_T
Cuts at: 3392 3392
Size: 3959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 193 2630 2814 193
Size: 2437 184 1338
Fragments arranged by size:
2437 1338 184
MscI TGG'CCA
Cuts at: 1720 1720
Size: 3959
MslI CAynn'nnrTG
Cuts at: 730 752 1948 2075 730
Size: 22 1196 127 2614
Fragments arranged by size:
2614 1196 127 22
MspA1I CmG'CkG
Cuts at: 1070 1384 1744 2757 3002 1070
Size: 314 360 1013 245 2027
Fragments arranged by size:
2027 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2140 2140
Size: 3959
NarI GG'CG_CC
Cuts at: 1390 1638 1390
Size: 248 3711
NgoMIV G'CCGG_C
Cuts at: 2138 2138
Size: 3959
NspI r_CATG'y
Cuts at: 1012 1512 2419 1012
Size: 500 907 2552
Fragments arranged by size:
2552 907 500
PciI A'CATG_T
Cuts at: 2415 2415
Size: 3959
PflMI CCAn_nnn'nTGG
Cuts at: 544 544
Size: 3959
PmeI GTTT'AAAC
Cuts at: 958 958
Size: 3959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 168 200 3098 3130 168
Size: 32 2898 32 997
Fragments arranged by size:
2898 997 32 32
PstI C_TGCA'G
Cuts at: 1006 1691 1006
Size: 685 3274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3959
PvuII CAG'CTG
Cuts at: 1384 1744 1384
Size: 360 3599
RsrII CG'GwC_CG
Cuts at: 2154 2154
Size: 3959
SacI G_AGCT'C
Cuts at: 973 973
Size: 3959
SalI G'TCGA_C
Cuts at: 996 996
Size: 3959
SapI GCTCTTCn'nnn_
Cuts at: 1982 2192 1982
Size: 210 3749
SbfI CC_TGCA'GG
Cuts at: 1006 1006
Size: 3959
ScaI AGT'ACT
Cuts at: 3476 3476
Size: 3959
SfcI C'TryA_G
Cuts at: 33 936 1002 1687 2680 2871 33
Size: 903 66 685 993 191 1121
Fragments arranged by size:
1121 993 903 685 191 66
SfoI GGC'GCC
Cuts at: 1391 1639 1391
Size: 248 3711
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3959
SmaI CCC'GGG
Cuts at: 981 981
Size: 3959
SmlI C'TyrA_G
Cuts at: 584 1114 2029 2521 2783 584
Size: 530 915 492 262 1760
Fragments arranged by size:
1760 915 530 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3959
SphI G_CATG'C
Cuts at: 1012 1512 1012
Size: 500 3459
StyI C'CwwG_G
Cuts at: 468 1093 1447 468
Size: 625 354 2980
Fragments arranged by size:
2980 625 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1542 1675 2134 2343 1542
Size: 133 459 209 3158
Fragments arranged by size:
3158 459 209 133
TatI w'GTAC_w
Cuts at: 491 3474 491
Size: 2983 976
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 232 264 3476 3508 232
Size: 32 3212 32 683
Fragments arranged by size:
3212 683 32 32
Tth111I GACn'n_nGTC
Cuts at: 1756 1756
Size: 3959
XbaI T'CTAG_A
Cuts at: 990 990
Size: 3959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1454 114
Size: 1340 2619
XhoI C'TCGA_G
Cuts at: 584 584
Size: 3959
XmaI C'CCGG_G
Cuts at: 979 979
Size: 3959
XmnI GAAnn'nnTTC
Cuts at: 402 402
Size: 3959
ZraI GAC'GTC
Cuts at: 805 805
Size: 3959
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PciI PflMI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BsgI BsiWI BsmI BssHII BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI