Restriction map for
pF1KE0684
(Circular) (Six-base) MAPSORT of: pF1KE0684.seq Check: 9614 from: 1 to: 4310
pF1KE0684 4310 bp
With 182 enzymes: SgfI *
April 24, 2009 13:17 ..
AatII G_ACGT'C
Cuts at: 410 410
Size: 4310
AccI GT'mk_AC
Cuts at: 1348 1348
Size: 4310
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 558 943 2135 2567 3314 3582 167
Size: 391 385 1192 432 747 268 895
Fragments arranged by size:
1192 895 747 432 391 385 268
AflII C'TTAA_G
Cuts at: 552 552
Size: 4310
AflIII A'CryG_T
Cuts at: 671 1265 1662 2766 3743 671
Size: 594 397 1104 977 1238
Fragments arranged by size:
1238 1104 977 594 397
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AlwNI CAG_nnn'CTG
Cuts at: 1615 3182 1615
Size: 1567 2743
ApaLI G'TGCA_C
Cuts at: 3080 3080
Size: 4310
ApoI r'AATT_y
Cuts at: 334 359 481 819 1314 3663 3919 334
Size: 25 122 338 495 2349 256 725
Fragments arranged by size:
2349 725 495 338 256 122 25
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 583 649 1194 1330 583
Size: 66 545 136 3563
Fragments arranged by size:
3563 545 136 66
BamHI G'GATC_C
Cuts at: 345 1335 345
Size: 990 3320
BanI G'GyrC_C
Cuts at: 1326 1740 1988 2023 1326
Size: 414 248 35 3613
Fragments arranged by size:
3613 414 248 35
BanII G_rGCy'C
Cuts at: 1019 1094 1201 1324 2354 1019
Size: 75 107 123 1030 2975
Fragments arranged by size:
2975 1030 123 107 75
BbeI G_GCGC'C
Cuts at: 1744 1992 1744
Size: 248 4062
BbsI GAAGACnn'nnnn_
Cuts at: 122 155 410 648 949 122
Size: 33 255 238 301 3483
Fragments arranged by size:
3483 301 255 238 33
BbvCI CC'TCA_GC
Cuts at: 814 1045 814
Size: 231 4079
BciVI GTATCCnnnnn_n'
Cuts at: 2208 2969 4310 2208
Size: 761 1341 2208
Fragments arranged by size:
2208 1341 761
BclI T'GATC_A
Cuts at: 1830 1830
Size: 4310
BglII A'GATC_T
Cuts at: 1825 3862 1825
Size: 2037 2273
BlpI GC'TnA_GC
Cuts at: 1422 1422
Size: 4310
Bme1580I G_kGCm'C
Cuts at: 1935 2028 3084 1935
Size: 93 1056 3161
Fragments arranged by size:
3161 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 408 1522 1603 1938 4082 408
Size: 1114 81 335 2144 636
Fragments arranged by size:
2144 1114 636 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4310
Bpu10I CC'TnA_GC
Cuts at: 814 1045 814
Size: 231 4079
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 2365 2857 3155 1486
Size: 879 492 298 2641
Fragments arranged by size:
2641 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4014 4014
Size: 4310
BsaAI yAC'GTr
Cuts at: 674 681 1663 674
Size: 7 982 3321
Fragments arranged by size:
3321 982 7
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 407 1741 1989 2691 4187 407
Size: 1334 248 702 1496 530
Fragments arranged by size:
1496 1334 702 530 248
BsaWI w'CCGG_w
Cuts at: 1181 1499 2020 2698 2972 3119 3530 1181
Size: 318 521 678 274 147 411 1961
Fragments arranged by size:
1961 678 521 411 318 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 923 953 923
Size: 30 4280
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 298 820 1219 1228 298
Size: 522 399 9 3380
Fragments arranged by size:
3380 522 399 9
BseYI C'CCAG_C
Cuts at: 927 1102 1735 2493 3070 3566 927
Size: 175 633 758 577 496 1671
Fragments arranged by size:
1671 758 633 577 496 175
BsiEI CG_ry'CG
Cuts at: 104 906 1152 1852 1898 3106 104
Size: 802 246 700 46 1208 1308
Fragments arranged by size:
1308 1208 802 700 246 46
BsiHKAI G_wGCw'C
Cuts at: 1094 1324 2102 2292 3084 1094
Size: 230 778 190 792 2320
Fragments arranged by size:
2320 792 778 230 190
Bsp1286I G_dGCh'C
Cuts at: 1019 1094 1201 1324 1935 2028 2102 2292 2354
Size: 75 107 123 611 93 74 190 62
Cuts at: 2354 3084 1019
Size: 730 2245
Fragments arranged by size:
2245 730 611 190 123 107 93 75
74 62
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 392 1107 1360 1574 1876 2257 392
Size: 715 253 214 302 381 2445
Fragments arranged by size:
2445 715 381 302 253 214
BsrBI CCG'CTC
Cuts at: 50 2602 2656 4140 4305 50
Size: 2552 54 1484 165 55
Fragments arranged by size:
2552 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2222 2222
Size: 4310
BsrFI r'CCGG_y
Cuts at: 956 2308 2489 2698 956
Size: 1352 181 209 2568
Fragments arranged by size:
2568 1352 209 181
BsrGI T'GTAC_A
Cuts at: 543 543
Size: 4310
BssSI C'ACGA_G
Cuts at: 2581 2939 2581
Size: 358 3952
BstAPI GCAn_nnn'nTGC
Cuts at: 1687 1687
Size: 4310
BstBI TT'CG_AA
Cuts at: 2671 2671
Size: 4310
BstYI r'GATC_y
Cuts at: 1 345 854 1184 1251 1335 1802 1825 2160
Size: 344 509 330 67 84 467 23 335
Cuts at: 2160 2406 3418 3504 3516 3862 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 509 467 449 346 344 335 330
246 86 84 67 23 12
Bsu36I CC'TnA_GG
Cuts at: 803 803
Size: 4310
BtgI C'CryG_G
Cuts at: 107 320 746 774 107
Size: 213 426 28 3643
Fragments arranged by size:
3643 426 213 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 747 2260 2441 2604 747
Size: 1513 181 163 2453
Fragments arranged by size:
2453 1513 181 163
BtsI GCAGTG_nn'
Cuts at: 1553 1553
Size: 4310
DraI TTT'AAA
Cuts at: 1309 1309
Size: 4310
DraIII CAC_nnn'GTG
Cuts at: 732 732
Size: 4310
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 1149 1895 2069 2460 2487 3832 1149
Size: 746 174 391 27 1345 1627
Fragments arranged by size:
1627 1345 746 391 174 27
EagI C'GGCC_G
Cuts at: 1149 1895 1149
Size: 746 3564
EarI CTCTTCn'nnn_
Cuts at: 158 2333 2543 158
Size: 2175 210 1925
Fragments arranged by size:
2175 1925 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2518 2828 2974 4120 2518
Size: 310 146 1146 2708
Fragments arranged by size:
2708 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1092 1322 1092
Size: 230 4080
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 558 943 2135 2567 3314 3582 167
Size: 391 385 1192 432 747 268 895
Fragments arranged by size:
1192 895 747 432 391 385 268
EcoNI CCTnn'n_nnAGG
Cuts at: 802 802
Size: 4310
EcoO109I rG'GnC_Cy
Cuts at: 795 1065 1449 795
Size: 270 384 3656
Fragments arranged by size:
3656 384 270
EcoRI G'AATT_C
Cuts at: 359 1314 359
Size: 955 3355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 4310
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 957 1744 1992 3014 3993 957
Size: 787 248 1022 979 1274
Fragments arranged by size:
1274 1022 979 787 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 252 284 2152 2182 2184 2214 252
Size: 32 1868 30 2 30 2348
Fragments arranged by size:
2348 1868 32 30 30 2
HincII GTy'rAC
Cuts at: 1349 1349
Size: 4310
KasI G'GCGC_C
Cuts at: 1740 1988 1740
Size: 248 4062
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1016 2981 3165 1016
Size: 1965 184 2161
Fragments arranged by size:
2161 1965 184
MscI TGG'CCA
Cuts at: 2071 2071
Size: 4310
MslI CAynn'nnrTG
Cuts at: 700 766 2299 2426 700
Size: 66 1533 127 2584
Fragments arranged by size:
2584 1533 127 66
MspA1I CmG'CkG
Cuts at: 776 1421 1735 2095 3108 3353 776
Size: 645 314 360 1013 245 1733
Fragments arranged by size:
1733 1013 645 360 314 245
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 1741 1989 1741
Size: 248 4062
NcoI C'CATG_G
Cuts at: 107 320 107
Size: 213 4097
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NspI r_CATG'y
Cuts at: 1269 1363 1863 2770 1269
Size: 94 500 907 2809
Fragments arranged by size:
2809 907 500 94
PasI CC'CwG_GG
Cuts at: 1019 1019
Size: 4310
PciI A'CATG_T
Cuts at: 1265 2766 1265
Size: 1501 2809
PfoI T'CCnGG_A
Cuts at: 857 857
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PmlI CAC'GTG
Cuts at: 674 674
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PpuMI rG'GwC_Cy
Cuts at: 795 1065 795
Size: 270 4040
PshAI GACnn'nnGTC
Cuts at: 188 188
Size: 4310
PsiI TTA'TAA
Cuts at: 425 425
Size: 4310
PstI C_TGCA'G
Cuts at: 1357 2042 1357
Size: 685 3625
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 1735 2095 1735
Size: 360 3950
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 1094 1324 1094
Size: 230 4080
SacII CC_GC'GG
Cuts at: 777 777
Size: 4310
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SfcI C'TryA_G
Cuts at: 33 388 1353 2038 3031 3222 33
Size: 355 965 685 993 191 1121
Fragments arranged by size:
1121 993 965 685 355 191
SfoI GGC'GCC
Cuts at: 1742 1990 1742
Size: 248 4062
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SgrAI Cr'CCGG_yG
Cuts at: 956 956
Size: 4310
SmaI CCC'GGG
Cuts at: 585 651 1332 585
Size: 66 681 3563
Fragments arranged by size:
3563 681 66
SmlI C'TyrA_G
Cuts at: 552 1465 2380 2872 3134 552
Size: 913 915 492 262 1728
Fragments arranged by size:
1728 915 913 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 1363 1863 1363
Size: 500 3810
SspI AAT'ATT
Cuts at: 489 489
Size: 4310
StuI AGG'CCT
Cuts at: 437 1129 437
Size: 692 3618
StyI C'CwwG_G
Cuts at: 107 320 1444 1798 107
Size: 213 1124 354 2619
Fragments arranged by size:
2619 1124 354 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 457 988 1893 2026 2485 2694 457
Size: 531 905 133 459 209 2073
Fragments arranged by size:
2073 905 531 459 209 133
TatI w'GTAC_w
Cuts at: 543 3825 543
Size: 3282 1028
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 923 955 3827 3859 923
Size: 32 2872 32 1374
Fragments arranged by size:
2872 1374 32 32
Tth111I GACn'n_nGTC
Cuts at: 2107 2107
Size: 4310
XbaI T'CTAG_A
Cuts at: 180 1341 180
Size: 1161 3149
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1805 1805
Size: 4310
XmaI C'CCGG_G
Cuts at: 583 649 1330 583
Size: 66 681 3563
Fragments arranged by size:
3563 681 66
XmnI GAAnn'nnTTC
Cuts at: 176 176
Size: 4310
ZraI GAC'GTC
Cuts at: 408 408
Size: 4310
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BpmI
BsgI BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PspOMI
PspXI PsrI SanDI SexAI SfiI SnaBI SrfI SwaI
XhoI